Resident and recruited macrophages differentially contribute to cardiac healing after myocardial ischemia

  1. Tobias Weinberger
  2. Messerer Denise
  3. Markus Joppich
  4. Maximilian Fischer
  5. Clarisabel Garcia Rodriguez
  6. Konda Kumaraswami
  7. Vanessa Wimmler
  8. Sonja Ablinger
  9. Saskia Räuber
  10. Jiahui Fang
  11. Lulu Liu
  12. Wing Han Liu
  13. Julia Winterhalter
  14. Johannes Lichti
  15. Lukas Thomas
  16. Dena Esfandyari
  17. Guelce Percin
  18. Sandra Matin
  19. Andrés Hidalgo
  20. Claudia Waskow
  21. Stefan Engelhardt
  22. Andrei Todica
  23. Ralf Zimmer
  24. Clare Pridans
  25. Elisa Gomez Perdiguero
  26. Christian Schulz  Is a corresponding author
  1. Medical Clinic I., Department of Cardiology, University Hospital, Ludwig Maximilian University, Germany
  2. Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine University, Germany
  3. DZHK (German Centre for Cardiovascular Research), Partner site Munich Heart Alliance, Germany
  4. Institut Pasteur, Unité Macrophages et Développement de l'Immunité, Département de Biologie du Développement et Cellules Souches, France
  5. LFE Bioinformatik, Department of Informatics, Ludwig Maximilian University, Germany
  6. Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Germany
  7. Institute of Pharmacology and Toxicology, Technical University Munich, Germany
  8. Immunology of Aging, Leibniz-Institute on Aging - Fritz-Lipmann-Institute (FLI), Germany
  9. Area of Cell & Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Spain
  10. Vascular Biology and Therapeutics Program and Department of Immunobiology, Yale University School of Medicine, United States
  11. Faculty of Biological Sciences, Friedrich-Schiller-University, Germany
  12. Department of Nuclear Medicine, Ludwig Maximilian University, Germany
  13. Simons Initiative for the Developing Brain, Centre for Discovery Brain Sciences, University of Edinburgh, United Kingdom
  14. University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, United Kingdom
  15. Department of Immunopharmacology, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Germany

Peer review process

Version of Record: This is the final version of the article.

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Editors

Senior Editor
  1. Olujimi A Ajijola
  2. University of California, Los Angeles, United States
Reviewing Editor
  1. Florent Ginhoux
  2. Singapore Immunology Network, Singapore

Reviewer #1 (Public Review):

Weinberger et al. use different fate-mapping models, the FIRE model and PLX-diet to follow and target different macrophage populations and combine them with single-cell data to understand their contribution to heart regeneration after I/R injury. This question has already been addressed by other groups in the field using different models. However, the major strength of this manuscript is the usage of the FIRE mouse model that, for the first time, allows specific targeting of only fetal-derived macrophages.

The data show that the absence of resident macrophages is not influencing infarct size but instead is altering the immune cell crosstalk in response to injury, which is in line with the current idea in the field that macrophages of different origins have distinct functions in tissues, especially after an injury.

To fully support the claims of the study, specific targeting of monocyte-derived macrophages or the inhibition of their influx at different stages after injury would be of high interest.

In summary, the study is well done and important for the field of cardiac injury. But it also provides a novel model (FIRE mice + RANK-Cre fate-mapping) for other tissues to study the function of fetal-derived macrophages while monocyte-derived macrophages remain intact.

https://doi.org/10.7554/eLife.89377.4.sa1

Reviewer #2 (Public Review):

In this study Weinberger et al. investigated cardiac macrophage subsets after ischemia/reperfusion (I/R) injury in mice. The authors studied a ∆FIRE mouse model (deletion of a regulatory element in the Csf1r locus), in which only tissue resident macrophages might be ablated. The authors showed a reduction of resident macrophages in ∆FIRE mice and characterized its macrophages populations via scRNAseq at baseline conditions and after I/R injury. 2 days after I/R protocol ∆FIRE mice showed an enhanced pro inflammatory phenotype in the RNAseq data and differential effects on echocardiographic function 6 and 30 days after I/R injury. Via flow cytometry and histology the authors confirmed existing evidence of increased bone marrow-derived macrophage infiltration to the heart, specifically to the ischemic myocardium. Macrophage population in ∆FIRE mice after I/R injury were only changed in the remote zone. Further RNAseq data on resident or recruited macrophages showed transcriptional differences between both cell types in terms of homeostasis-related genes and inflammation. Depleting all macrophage using a Csf1r inhibitor resulted in a reduced cardiac function and increased fibrosis.

Strengths:

(1) The authors utilized robust methodology encompassing state of the art immunological methods, different genetic mouse models and transcriptomics.

(2) The topic of this work is important given the emerging role of tissue resident macrophages in cardiac homeostasis and disease.

Comments on revised version:

The authors have responded to all questions. I have no further comments and congratulate the authors on their work.

https://doi.org/10.7554/eLife.89377.4.sa2

Author response

The following is the authors’ response to the previous reviews.

Recommendations for the authors:

Reviewer #1 (Recommendations For The Authors):

The authors have addressed most of the points that were made. However, despite some things that may well be beyond the scope, I would like to insist on a few small points:

Point 1: If the authors have conducted a gross analysis of cardiac morphology by histology already, they should include this data in the manuscript and comment with 1-2 sentences that "cardiac healing"..."is unlikely influenced by developmental defects".

We agree with the reviewer that this analysis is important. Therefore, we are currently conducting an in-depth analysis of the cardiac phenotype of different mouse strains lacking distinct subpopulations of cardiac macrophages in development and non-stimulated (baseline) conditions, including functional, metabolic and even electrophysiological aspects. These analysis will also include FIRE mice. While a gross analysis in this mouse strain did not show pathologic aspects, we look forward to the very detailed tissue characterization before publishing any data from a first basic analysis.

Point 7: There is still no legend in Figure 6: what is read? What is blue?

We added the respective legend in the figure.

Point 8: Please add the information on the background of mice used for the different FIRE mice into the methods part of the paper

We added the information in the Methods Part (lines 344-347).

Reviewer #2 (Recommendations For The Authors):

The authors have responded to all questions. I have no further comments and congratulate the authors on their work.

We thank the reviewer for their important questions and the constructive feedback.

https://doi.org/10.7554/eLife.89377.4.sa3

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  1. Tobias Weinberger
  2. Messerer Denise
  3. Markus Joppich
  4. Maximilian Fischer
  5. Clarisabel Garcia Rodriguez
  6. Konda Kumaraswami
  7. Vanessa Wimmler
  8. Sonja Ablinger
  9. Saskia Räuber
  10. Jiahui Fang
  11. Lulu Liu
  12. Wing Han Liu
  13. Julia Winterhalter
  14. Johannes Lichti
  15. Lukas Thomas
  16. Dena Esfandyari
  17. Guelce Percin
  18. Sandra Matin
  19. Andrés Hidalgo
  20. Claudia Waskow
  21. Stefan Engelhardt
  22. Andrei Todica
  23. Ralf Zimmer
  24. Clare Pridans
  25. Elisa Gomez Perdiguero
  26. Christian Schulz
(2024)
Resident and recruited macrophages differentially contribute to cardiac healing after myocardial ischemia
eLife 12:RP89377.
https://doi.org/10.7554/eLife.89377.4

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https://doi.org/10.7554/eLife.89377