Figure 2—figure supplement 1. | RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

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RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

Figure 2—figure supplement 1.

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University of California, San Francisco, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Rev interactions in the free and RNA-bound structures.

(A) Model illustrating the 70° rotation that one subunit of the unbound Rev undergoes in order to contact the RNA at the junction site, pivoting around Ile55 (yellow) (B) View of the dimer interface from the top (RNA-binding side) showing that the second Rev molecule in the RNA-bound dimer is situated ∼3.2 Å further away from the first Rev subunit than in the RNA-free arrangement (C) Gel-shift assay using 32P-labeled full-length RRE (234 nucleotides) and full-length Rev illustrating the modest loss of binding affinity upon mutating Gln51 to Ala. Apparent Kd and n are: wild-type Rev Kd = 145 pM, n = 2.1; Q51A Rev Kd = 280 pM, n = 1.7.

DOI: http://dx.doi.org/10.7554/eLife.04120.009