• Figure 6.
    Download figureOpen in new tabFigure 6. Visualization of impaired whole-body coordination in pcd.

    (A) Ribbon plots showing average vertical (z) trajectories for nose, paw and select tail (2, 5, 8, 11, 14) segments for size-matched control (Left, N = 11)) and pcd (Right, N = 3) mice walking at 0.20–0.25 m/s. Data are presented relative to 100% of the stride cycle of the FR paw (x-axis). Nose and paw trajectories are z position relative to floor; tail is z relative to floor with mean vertical position of the of base of the tail subtracted for clarity. (B) Matrix of correlation coefficients computed for average vertical trajectories of control (Left) and pcd (Right). Color bar is value of correlation coefficient.

    DOI: http://dx.doi.org/10.7554/eLife.07892.019

  • Video 1. Automated, high-resolution locomotion tracking in freely walking mice.

    High-speed (400 fps) video of a mouse crossing the LocoMouse corridor, displayed at 30 fps. Side and bottom (via mirror reflection) views of the mouse are captured in a single camera. Top: Raw video of a wild-type mouse. Bottom: Same video with the output of the machine learning tracking overlaid: nose (orange circle), paws (red: front right; blue: front left; magenta: hind right; cyan: hind left) and tail segments (green-to-yellow gradient circles).

    DOI: http://dx.doi.org/10.7554/eLife.07892.005

  • Video 2. Purkinje cell degeneration mice are visibly ataxic.

    Purkinje cell degeneration (pcd) mouse crossing the LocoMouse corridor. Pcd mice are smaller and walk more slowly than controls. They lift their paws higher and have altered patterns of interlimb coordination. The nose and tail oscillate laterally and vertically.

    DOI: http://dx.doi.org/10.7554/eLife.07892.009

  • Video 3. Passive nose and tail model.

    Passive model of nose and tail for a mouse walking at 0.2 m/s. The forward movements of the paws were modeled according to the data described in Figures 3 and 4. The lateral movements of the nose and tail segments are predicted from a model in which an orthogonal projection transforms the forward movements of the hind paws (solid white line) into lateral movements of the tail (dashed white line) and nose with a fixed time-delay for each element. As a result of this orthogonal coupling, the nose and tail oscillate laterally as a passive consequence of the forward motion of the hind paws.

    DOI: http://dx.doi.org/10.7554/eLife.07892.018