Figure 1—figure supplement 1. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 1—figure supplement 1.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 1—figure supplement 1.
Download figureOpen in new tabFigure 1—figure supplement 1. RNA-seq validation of control genes in chd4 and p53 RNAi animals.

(A) Colorimetric WISH was performed on control, chd4 and p53 RNAi animals for smedwi-1 (stem cell marker), prog-1 (early progeny) and AGAT-1 (late progeny) from the same cohort of worms used for RNA-seq analysis. Representative images of 3Fd15 RNAi worms are shown. Scale bar: 200 μm. (B) Plot of log2 ratios of control genes (smedwi-1, prog-1, AGAT-1) and targeted RNAi genes (chd4 and p53) in chd4(RNAi) (top panel) and p53(RNAi) (bottom panel) time course. For each time point, reads mapping to gene models were counted in chd4(RNAi) and p53(RNAi) data sets and were then compared to the unc22(RNAi) control, and log2 ratios were generated. (C-D) Volcano plots illustrating genes significantly down-regulated (green dots; adjusted p-value <1e-13, log2 <-1) and up-regulated (red dots; adjusted p-value <1e-13, log2 >1) at 3Fd15 in chd4(RNAi) data set (C) and at d18 in p53(RNAi) data set (D). Control genes are highlighted in yellow. Blue dots represent genes from the 587 gene overlap data set (Figure 1D) that are also significantly down-regulated in the plotted time points. See also Supplementary file 1. See Supplementary file 2 for up-regulated gene list.

DOI: http://dx.doi.org/10.7554/eLife.10501.004