Figure 6—figure supplement 1. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 6—figure supplement 1.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 6—figure supplement 1.
Download figureOpen in new tabFigure 6—figure supplement 1. Analysis of multiple dividing tissue progenitors in egr-5(RNAi) animals.

(A) Representative images of egr-5(RNAi) animals from Figure 6C–G fixed and stained for H3P (red), neoblast marker (blue: smedwi-1) and progenitor marker (green). Progenitor markers: zeta-class/epidermal: zfp-1, egr-1, fgfr-1; protonephridia: pou2/3; gut: hnf4; brain: pax6A. Images represent whole-animal maximum intensity projections and zoomed image highlights an example of a positive dividing progenitor cell (i.e. H3P+/smedwi-1+/progenitor marker+). Scale bars: 200 μm. (B-D) Expansion of multiple differentiated tissue markers in egr-5(RNAi) animals. Representative colorimetric WISH images of control and egr-5(RNAi) intact animals (6Fd25) monitoring various lineages/tissues: protonephridia (pou2/3), epidermal progenitors and early/late progeny cells (p53), and gut morphology/gut progenitors (hnf4). Scale bars: 200 μm.

DOI: http://dx.doi.org/10.7554/eLife.10501.022