Table 3. | Genomic DNA transposition induced by human PGBD5

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Genomic DNA transposition induced by human PGBD5

Table 3.

Affiliation details

Memorial Sloan Kettering Cancer Center, United States; Weill Cornell Medical College, United States; Harvard Medical School, United States; University of Utah School of Medicine, United States; Cornell University, United States
Table 3.

Analysis of transposon integration sequences in human genomes induced by PGBD5

DOI: http://dx.doi.org/10.7554/eLife.10565.018

Intact transposonMutant transposon
TTAA ITRNon-ITRTTAA ITRNon-ITR
Transposase
 GFP-PGBD582% (65)18% (14)11% (4)89% (33)
 GFP Control17% (2)83% (10)40% (27)60% (40)
  • Cells expressing GFP-PGBD5 and intact transposons exhibit significantly higher frequency of genomic integration as compared to either GFP control, or GFP-PGBD5 with mutant transposons, with 82% (65 out of 79) of sequences demonstrating DNA transposition of ITR transposons into TTAA sites (†p = 1.8 × 10-5). Mutation of the transposon ITR significantly reduces ITR-mediated integration, with only 11% (4 out of 37) of sequences (‡p = 0.0016). Numbers in parentheses denote absolute numbers of identified insertion sites.

  • GFP, green fluorescent protein; ITR, inverted terminal repeat.