3,419 results found
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain

    Tianyu Wang, Chunyan Wu ... Chris Xu
    Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    Identification of distinct pH- and zeaxanthin-dependent quenching in LHCSR3 from Chlamydomonas reinhardtii

    Julianne M Troiano, Federico Perozeni ... Gabriela S Schlau-Cohen
    Light-harvesting complex stress-related is a protein from photosynthetic green algae that prevents damage from sunlight via two distinct conformational processes, which protect against different timescales of solar fluctuations.
    1. Structural Biology and Molecular Biophysics

    Inhibition of the proton-activated chloride channel PAC by PIP2

    Ljubica Mihaljević, Zheng Ruan ... Zhaozhu Qiu
    PIP2 inhibits the proton-activated chloride (PAC) channel by selectively stabilizing its desensitized state.
    1. Neuroscience

    Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle

    Max Jameson Aragon, Aaron T Mok ... Nilay Yapici
    Development of imaging methods to capture neural activity and structure through the intact cuticle using 2-photon and 3-photon excitation in the genetic model organism, Drosophila melanogaster.
    1. Neuroscience

    Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses

    Bernard Bloem, Rafiq Huda ... Ann M Graybiel
    Simultaneous 2-photon imaging of striosomes and matrix in mice shows that striosomes preferentially encode reward-predicting cues whereas both striatal compartments demonstrate reward-related activity.
    1. Structural Biology and Molecular Biophysics

    Structural dynamics determine voltage and pH gating in human voltage-gated proton channel

    Shuo Han, Sophia Peng ... Shizhen Wang
    Visualization of the real-time conformational transitions of the human voltage-gated proton channel hHv1 provided novel insights into how voltage and pH gradients modify the dynamic behaviors of channel structures to control proton flow across membrane.
    1. Neuroscience

    Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

    Andrea Antonini, Andrea Sattin ... Tommaso Fellin
    Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae

    Yann Gambin, Nicholas Ariotti ... Robert G Parton
    New insights into the assembly and membrane interactions of the caveolar coat complex reveal the reversible association/dissociation of distinct subcomplexes onto the membrane, which differs from the assembly/disassembly of clathrin-coated pits.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    pH- and sodium-induced changes in a sodium/proton antiporter

    Cristina Paulino, Werner Kühlbrandt
    Sodium ions but not protons induce defined helix movements in a sodium/proton antiporter and suggest a mechanism for Na + binding and transport.
    1. Neuroscience

    Network instability dynamics drive a transient bursting period in the developing hippocampus in vivo

    Jürgen Graf, Vahid Rahmati ... Knut Kirmse
    Two-photon Ca2+ imaging and computational modeling reveal major developmental trajectories of spontaneous activity in developing CA1 and identify important roles of network bi-stability and synaptic input characteristics for hippocampal burstiness before eye opening.

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