The Foxa2 lineage-based organ generative strategy allows us to establish a unique paradigm for the simultaneous generation of functional whole lungs and thymus, which can be applied to future transplantation therapy.

A combination of unbiased scRNA-sequencing and functional approaches in organoid cultures and injury-repair models in vivo reveals major heterogeneity in adult murine airway basal cells, which is established prenatally and conserved in humans.

Live imaging, transcriptional profiling and mouse models demonstrated that the mesenchyme controls the growth pace but not the mode of branching of the mammary epithelium and unveiled a unique function for Igf1/Igfr1 in embryonic development mammary gland.

Inhibition of a single fibroblast-associated transcription factor, namely Paired-related Homeobox Protein 1 (PRRX1), is sufficient to dampen lung fibrogenesis.

Lung stem cell niche aging is associated with altered bioenergetics, metabolic dysfunction, and senescence of mesenchymal stromal cells that restricts 3D organoid, or alveolosphere formation, potentially accounting for reduced regenerative capacity with aging.

Embryonic macrophages encourage early kidney development, interact with developing renal blood vessels, are enriched for mRNAs linked to vascular development, and promote endothelial cross-connections.

Cell-cell interaction between neural crest-derived cells and mesoderm-derived cells determine cell fate of mesoderm-derived cell in tongue development through Hh signaling.

Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.

Multimodal technologies and the experimental tractability of sea urchin embryos reveal diverse regulatory mechanisms and unique genes for distinct pigment cell states.