Structural and computational approaches uncover the molecular basis for dimerization, open-closed conformational transitions, and the dynamic behavior of the human angiotensin-I converting enzyme dimer.
Piezo proteins induce a curved protein-membrane nanodome with an excess area of about 40 nm2 in tensionless membranes and resist tension-induced flattening with a force constant of about 60 pN/nm.
Membrane geometry switches protein condensate organization of postsynaptic density proteins by reducing crowding effects while maintaining specific binding, allowing targeted interactions to dominate over size-based exclusion.
Grafting surface loops containing antibody epitopes of a poorly expressed flu neuraminidase protein onto a high-expressing, stable scaffold improves its production and stability for vaccine development.
Ten-microsecond atomistic simulations directly capture the voltage activation of the Core-MT big potassium channel, revealing novel voltage sensing and sensor-pore coupling mechanisms that have largely eluded the community.
The crystal structure of Sld3CBD-Cdc45 shows its intermolecular interactions, and subsequent Sld3CBD-CMG model construction and binding assays enable a proposal for the mechanism of Cdc45 recruitment and Sld3 release.
