A novel F-actin-binding motif consisting of an α-helix hairpin from a Legionella pneumophila lysine fatty acyltransferase has the potential to be developed as an F-actin probe.
Clinically relevant Ω-loop mutations in PDC-3 reshape active-site dynamics to enhance β-lactamase activity, providing mechanistic insights that can guide the rational design of inhibitors.
Liudmyla Arifova, Brian S MacTavish ... Douglas J Kojetin
Covalent PPARγ inhibitors that better stabilize a repressive conformation are more effective inhibitors, but structural plasticity still allows ligand cobinding by enabling a shift back to an active conformation.
Formation and stability of endocytic receptor-arrestin complexes during clathrin-mediated endocytosis are governed by phosphorylation-dependent arrestin-AP2 interactions.
BK and CaV1.3 channels interact early during biogenesis, assembling intracellularly before membrane localization, indicating coordinated processes for functional coupling.
Modestas Matusevicius, Robin A Corey ... Simone Weyand
Cryo-EM structures of human aquaporin AQP2 bound to the anti-trypanosomal drugs pentamidine and melarsoprol identify the molecular mechanism for drug-resistant sleeping sickness.
Single-molecule tracking of transcription factors in living cells revealed how mutations that make short activation domains stronger increased the fraction of transcription factor molecules bound to chromatin and led to longer residence times on chromatin.
The stability of ribonucleoprotein complexes of SARS-CoV-2 nucleocapsid proteins differs among variants of concern and the viral evolution of nucleocapsid protein can be understood in the framework of fuzzy complexes.
Marta Wojnowska, Takeaki Wajima ... Ulrich Schwarz-Linek
The T-shaped fold of an abundant surface protein ties streptococci to collagens, an interaction that enhances biofilm such as often found in invasive infections.
