Browse our latest Structural Biology and Molecular Biophysics articles

Page 28 of 177
    1. Structural Biology and Molecular Biophysics

    Structural insights into regulation of CNNM-TRPM7 divalent cation uptake by the small GTPase ARL15

    Luba Mahbub, Guennadi Kozlov ... Kalle Gehring
    ADP-ribosylation factor-like GTPase 15, an atypical small GTPase, binds to cystathionine-β-synthase-pair domain divalent metal cation transport mediator (CNNM) membrane proteins to inhibit divalent cation efflux by CNNM proteins and influx by transient receptor potential ion channel subfamily M member 7.
    1. Structural Biology and Molecular Biophysics

    Sterol derivative binding to the orthosteric site causes conformational changes in an invertebrate Cys-loop receptor

    Steven De Gieter, Casey I Gallagher ... Rouslan G Efremov
    Cryo-EM structures of Cys-loop receptor Alpo4 from the thermophilic worm Alvinella pompejana show that a sterol derivative CHAPS binds in and outside of its orthosteric binding site and induces a quaternary twist.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    A novel single alpha-helix DNA-binding domain in CAF-1 promotes gene silencing and DNA damage survival through tetrasome-length DNA selectivity and spacer function

    Ruben Rosas, Rhiannon R Aguilar ... Mair EA Churchill
    The lysine/glutamic acid/arginine region in chromatin assembly factor (CAF-1) forms a long single alpha-helix DNA-binding domain, facilitating the recognition of tetrasome-length DNA and linking functional domains within the CAF-1 architecture for efficient tetrasome assembly after DNA synthesis.
    1. Structural Biology and Molecular Biophysics

    Mechanistic insights into robust cardiac IKs potassium channel activation by aromatic polyunsaturated fatty acid analogues

    Briana M Bohannon, Jessica J Jowais ... H Peter Larsson
    Changing substituents on the aromatic rings of polyunsaturated fatty acid analogues tailors their activation effect on the IKs channel and thus their therapeutic potential for long QT syndrome.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Allosteric activation or inhibition of PI3Kγ mediated through conformational changes in the p110γ helical domain

    Noah J Harris, Meredith L Jenkins ... John E Burke
    Regulation of phosphoinositide 3 kinase (PI3Kγ) is essential in immune function, and stimuli that modulate the dynamics of the PI3Kγ helical domain can activate or inhibit kinase activity.
    1. Structural Biology and Molecular Biophysics

    Reconstructing the transport cycle in the sugar porter superfamily using coevolution-powered machine learning

    Darko Mitrovic, Sarah E McComas ... Lucie Delemotte
    Using state-specific contacts inferred from coevolving residue pairs enables us to build models of the various conformational states along the sugar porter functional cycle and reconstruct the free-energy landscape of the process.
    1. Structural Biology and Molecular Biophysics

    Free energy landscapes of KcsA inactivation

    Sergio Pérez-Conesa, Lucie Delemotte
    Not revised
    Reviewed Preprint v1
    • Valuable
    • Solid
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    Systematic analysis of the molecular and biophysical properties of key DNA damage response factors

    Joshua R Heyza, Mariia Mikhova ... Jens C Schmidt
    Live-cell single-molecule imaging of DNA repair factors can be used to analyze their localization to DNA lesions and dissect the hierarchy of their recruitment.
    1. Structural Biology and Molecular Biophysics

    Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels

    Ahmad Elbahnsi, John Cowgill ... Lucie Delemotte
    The HCN1 channel gates thanks to a coupling mechanism involving the reorganization of the interfaces between the voltage-sensor domains and pore helices, subtly shifting the balance between hydrophobic and hydrophilic interactions in a 'domino effect'.
    1. Structural Biology and Molecular Biophysics

    Agonist efficiency links binding and gating in a nicotinic receptor

    Dinesh C Indurthi, Anthony Auerbach
    Efficiency measures the fundamental link between agonist binding and protein conformational change, and in nicotinic receptors has 5 values that calibrate energy changes in the induced fit that triggers activation.