1. Genomics and Evolutionary Biology
  2. Microbiology and Infectious Disease
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Reciprocal virulence and resistance polymorphism in the relationship between Toxoplasma gondii and the house mouse

  1. Jingtao Lilue
  2. Urs Benedikt Müller
  3. Tobias Steinfeldt
  4. Jonathan C Howard Is a corresponding author
  1. University of Cologne, Germany
  2. Fundação Calouste Gulbenkian, Instituto Gulbenkian de Ciência, Portugal
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Cite as: eLife 2013;2:e01298 doi: 10.7554/eLife.01298

Abstract

Virulence in the ubiquitous intracellular protozoon Toxoplasma gondii for its natural intermediate host, the mouse, appears paradoxical from an evolutionary standpoint because death of the mouse before encystment interrupts the parasite life cycle. Virulent T. gondii strains secrete kinases and pseudokinases that inactivate the immunity-related GTPases (IRG proteins) responsible for mouse resistance to avirulent strains. Such considerations stimulated a search for IRG alleles unknown in laboratory mice that might confer resistance to virulent strains of T. gondii. We report that the mouse IRG system shows extraordinary polymorphic complexity in the wild. We describe an IRG haplotype from a wild-derived mouse strain that confers resistance against virulent parasites by interference with the virulent kinase complex. In such hosts virulent strains can encyst, hinting at an explanation for the evolution of virulence polymorphism in T. gondii.

https://doi.org/10.7554/eLife.01298.001

eLife digest

The parasite Toxoplasma gondii is one of the most common parasites worldwide and is known for its unusual life cycle. It reproduces sexually inside its primary host—the cat—and produces eggs that are released in faeces. Other animals, most often rodents, can then become infected when they unknowingly eat the eggs while foraging. Once inside its new host, the parasite reproduces asexually until the rodent’s immune system begins to fight back. It then becomes semi-dormant and forms cysts within the brain and muscle cells of its host. In an added twist, the parasite also causes rodents to lose their fear of cats. This increases their chances of being caught and eaten, thereby helping the parasite to return to its primary host and complete its life cycle.

Previous work has shown that virulent strains of T. gondii can evade the host immune system in mice by secreting enzymes that inactivate immune-related proteins called IRG proteins. This prevents the infection being cleared and leads to death of the host within a few days. The existence of these virulent strains is intriguing because parasites that kill their host, and thus prevent their own reproduction, should be eliminated from the population. The fact that they are fairly common suggests that there must be a hitherto unknown mechanism that allows rodents to survive these virulent strains.

Lilue et al. now report the existence of such a mechanism in strains of mice found in the wild. In contrast to laboratory mice, wild mice produce IRG proteins that inhibit the enzymes secreted by the virulent strains of T. gondii. Moreover, the IRG genes in wild mice are highly variable, whereas laboratory mice all have virtually identical IRG genes.

By uncovering the complexity and variability of IRG genes in wild mice—complexity that has been lost from laboratory strains—Lilue et al. solve the conundrum of how highly virulent T. gondii strains can persist in the mouse population, and offer an explanation for the evolution of parasitic strains with differing levels of virulence.

https://doi.org/10.7554/eLife.01298.002

Introduction

A virulent parasite that overcomes the immune system and kills its host may seem to have won the confrontation, but it is a Pyrrhic victory when the early death of the host reduces the probability of parasite transmission. Indeed it is in the interests of all hosts and most parasites to prolong the encounter. In this sense, virulence is a failure of co-adaptation. Haldane’s conjecture (Haldane, 1949) that intense and fluctuating selection imposed by parasites will generate host protein polymorphism is widely accepted (Woolhouse et al., 2002; Clark et al., 2007; Kosiol et al., 2008; Fumagalli et al., 2011). The presence in a population of multiple host resistance alleles confronting multiple parasite virulence alleles may reflect a dynamic equilibrium permissive for the persistence of both parties. However this equilibrium is achieved only at the expense of individual interactions fatal for either the parasite or the host, as a consequence of confrontations of inappropriate alleles. In mammals, however, the ability of the adaptive immune system to respond within the time scale of an individual infection and to remember for a lifetime, buffers individuals against dangerous genetic novelty arising from parasites. As a result, life or death outcomes for common infectious diseases in mammals are not generally determined by single, highly penetrant, polymorphic genes. We here report such a case, involving infection of the house mouse, Mus musculus, with the ubiquitous intracellular protozoan parasite, Toxoplasma gondii. T. gondii has a complex life cycle (Dubey, 1998) (Figure 1). The sexual process occurs in true cats (Felidae) and intermediate hosts become infected by ingesting oocysts spread in cat faeces. A phase of fast intracellular replication and spread (tachyzoite phase) stimulates immunity in the intermediate host, and this in turn induces parasite encystment in brain and muscle cells and lifelong persistence. Predation of the infected host by a cat completes the life cycle. If immunity fails, tachyzoite replication continues uninterrupted, killing the infected host within a few days (Deckert-Schlüter et al., 1996). Thus the probability that T. gondii completes its life cycle, which is roughly linear with duration of infection of the intermediate host, depends on early immune control.

The life cycle of T. gondii.

All warm blooded animals may serve as intermediate hosts, which are infected by ingestion of food or water contaminated by oocysts. Felids, the definitive hosts, are infected by ingesting tissue cysts from their prey. The intermediate phase of the life cycle may be prolonged by carnivory between intermediate hosts (not shown). Modified from free-license pictures.

https://doi.org/10.7554/eLife.01298.003

Mus musculus is probably the evolutionarily most important intermediate host for T. gondii, because it is very abundant worldwide and sympatric with a uniquely abundant felid, the domestic cat. Early immune control of T. gondii in mice depends on a family of IFNγ-inducible cytoplasmic effector proteins, the 47 kDa immunity-related GTPases (IRG proteins; for nomenclature of IRG genes and proteins see Bekpen et al. (2005); Martens and Howard (2006) and ‘Materials and methods’) (Taylor et al., 2000; Collazo et al., 2001; Liesenfeld et al., 2011). These assemble on the cytosolic face of the parasitophorous vacuole membrane (PVM), causing its rupture and killing the included parasite (Martens et al., 2005; Zhao et al., 2009b). In the C57BL/6 (BL/6) laboratory mouse strain about 20 IRG genes occur in two adjacent clusters on chromosome 11 and one cluster on chromosome 18 (Bekpen et al., 2005) (Figure 2A). The whole 47 kDa sequences of IRG proteins are typically translated from a single exon. Exceptional are certain ‘tandem’ IRGB proteins with a molecular weight of about 94 kDa. These genes are transcribed across two chromosomally adjacent IRG coding units and the intergenic spacer is spliced out as an intron resulting in a single open reading frame (Bekpen et al., 2005. See also ‘Materials and methods’ for a note on nomenclature of the tandem genes and proteins). IRG proteins fall into two major functional and sequence sub-families, the GKS group (IRGA, IRGB and IRGD proteins) that are effector proteins at the PVM, and the GMS group (Irgm1, Irgm2 and Irgm3) that are negative regulators of the GKS proteins (Hunn et al., 2008). Many strains of T. gondii (e.g., the abundant Eurasian strains designated types II and III) are well controlled by the IRG system in laboratory mice, encyst, and are considered avirulent. But others (e.g., type I) are highly virulent (Sibley and Boothroyd, 1992), killing the mouse host during the tachyzoite phase of infection. It has very recently been shown that virulence differences between T. gondii strains are largely due to polymorphic variation in ROP18 and ROP5 (Saeij et al., 2006; Taylor et al., 2006; Khan et al., 2009; Behnke et al., 2011), members of a family of kinases and pseudokinases (El Hajj et al., 2006). These proteins are secreted during parasite entry and accumulate on the cytosolic face of the PVM (Boothroyd and Dubremetz, 2008). Virulent ROP allotypes inactivate IRG effector proteins by phosphorylating essential threonines in the nucleotide-binding domain (Fentress et al., 2010; Steinfeldt et al., 2010). In view of the unique importance of Mus musculus for the transmission of T. gondii, parasite strains acutely lethal for mice should be counterselected. Their presence at a significant frequency therefore demands an explanation. In this paper, we reveal that the IRG resistance system in mice has a scale of polymorphic complexity that rivals the MHC, and that a resistant IRG haplotype in the mouse can counter polymorphic virulence factors in the parasite, generating a host phenotype that is permissive for encystment, and thus for the propagation, of virulent strains.

Figure 2 with 2 supplements see all
IRG protein polymorphism in inbred mouse strains.

(A) Linear order of IRG gene clusters on Chr 11 and Chr 18 of mouse strain BL/6. (B) Polymorphism at the protein level in the IRG genes of Chr 11 and Chr 18. Irga9Irga20 are absent from BL/6 and are inferred from resequencing data. Each colour block represents one IRG open reading frame and shows the number of amino acid substitutions/indels relative to the BL/6 allele. The colours of the blocks indicate their phylogenetic relationship (Figure 2—figure supplement 1). Open blocks in strains Czech II and CIM indicate homologues expected but not yet found. ‘ψ’ indicates probable pseudogenes. (C) Dot plots of the longer IRG gene clusters on Chr 11 in CAST/Ei and MSM/Ms against the BL/6 genomic sequence. Small blue squares show the positions of homologous coding units, blue lines indicate the positions of genes in BL/6 that are absent from the other genomes.

https://doi.org/10.7554/eLife.01298.004

Results

We compared the IRG genes from a number of mouse strains, mostly those re-sequenced in the Mouse Genomes Project (Keane et al., 2011) with the canonical BL/6 sequences (Figure 2B). The re-sequenced mice are largely from established laboratory strains. Others are recently derived from the wild and not admixed with laboratory mice. Mus spretus is a wild species distinct from Mus musculus with a divergent history of 1–3 million years (Suzuki et al., 2004). Within laboratory strains we found relatively little IRG protein polymorphism on Chr 11. In contrast, the wild-derived strains showed variation in IRG gene number and remarkable protein polymorphism. For example Irgb6, a protein of 406 residues, had an allele in the CAST/EiJ strain with 47 amino acid substitutions relative to BL/6. On Chr 18, IRG protein sequence variation between laboratory strains was more apparent, and again, wild-derived strains showed extensive sequence polymorphism and copy number variation. The IRG proteins of the outgroup, M. spretus, showed considerable protein divergence from M. musculus sequences. Pseudogenes occurred in every haplotype and some (e.g. Irga5 and Irgb7) were preserved between M. musculus and M. spretus. Other IRG sequences were pseudogenes in some haplotypes and apparently intact in others, for example Irga3 and Irga8. Even within this limited group of strains we can distinguish eleven distinct IRG gene haplotypes on Chr 11 and thirteen on Chr 18, already yielding a theoretical population diversity of several hundred IRG genotypes. Diagonal dot-plot comparisons between IRG gene clusters of wild-derived CAST/Ei and MSM/Ms with BL/6 showed IRG genes within tracts of duplication and deletion, associated with numerous repeats in both orientations, a genomic configuration that would be expected to be dynamic even on short time-scales, and doubtless responsible for the two very different dot-plots (Figure 2C and Figure 2—figure supplement 2).

We added sequences amplified from genomic DNA of wild mice from several Eurasian sites (Figure 3—figure supplement 1) to the data from inbred strains to generate nearest neighbour phylograms (Figure 3A). We analysed five IRG genes, namely Irgm1, a regulatory IRG protein of the GMS class (Hunn et al., 2008), and Irga6, Irgb2, Irgb6, and Irgb10, all effector GKS proteins localizing to the T. gondii PVM during infection (Khaminets et al., 2010). Irgb2 forms the N-terminal half of the tandem protein, Irgb2-b1. The nearest-neighbour phylograms of Irgm1, Irgb10 and Irga6 are shallow and the M. spretus sequences fall into outgroups. Thus most of the sparse polymorphic variation in these sequences has been acquired since the divergence of M. musculus from M. spretus. This conclusion is supported by a tendency for individual minor variants to be concentrated in one or other of the three recent, geographically separated and partially isolated subspecies, Mus musculus musculus, Mus musculus domesticus and Mus musculus castaneus (Figure 3A, Figure 3—figure supplements 2–4). In contrast, the phylograms for Irgb2 and Irgb6 have a depth similar to the mouse-rat divergence (about 20 million years) (Gibbs et al., 2004), the M. spretus sequences are embedded in the M. musculus trees, and any tendency to correlation with subspecies is seen only in the outermost branches. Thus the polymorphism in these two genes is ancient and has persisted through a number of speciation events. The scale of polymorphism in the IRG system estimated by Tajima’s π from 7 mouse strains resembles that of classical MHC genes (Figure 3B). That there is also considerable local polymorphism within geographic ranges was confirmed by the identification of numerous heterozygotes at all loci examined by PCR among the wild mouse captures.

Figure 3 with 4 supplements see all
Polymorphism of five IRG genes.

(A) Phylogenetic trees of five IRG genes sequenced from DNA of wild mice collected from various sites in Eurasia. Green, blue and red taxa represent M. m. domesticus, M. m. musculus and M. m. castaneus samples respectively. The black taxon represents Mus spretus. Alleles found in heterozygous condition in certain mice are indicated by numbers appended to individual mouse identifiers (some haplotypes contain 2 Irgb6 paralogous genes (Figure 2B), hence potentially up to 4 alleles). Bootstrap values are shown if >90. The sequences are avaliable in (Figure 3—source data 2-6). (B) The nucleotide pairwise diversities (π) of genes across seven laboratory and wild-derived inbred mouse strains (BL/6, AKR/J, MSM/Ms, CAST/Ei, PWK/PhJ, WSB/Ei and Spretus/EiJ). Grey bars indicate the distribution of π from 50 ‘random’ genes (Figure 3—source data 1). The π values of individual IRG and MHC genes are indicated by arrows.

https://doi.org/10.7554/eLife.01298.007

With such striking polymorphic diversity in the sequences of proteins known to be involved in resistance against T. gondii, it was appropriate to assay the ability of different IRG genotypes to resist virulent T. gondii. The wild-derived Indian strain CIM, which has a number of divergent IRG alleles (Figure 2B), proved to be remarkably resistant to the type I virulent T. gondii strain, GT-1 (Figure 4A). All CIM mice survived intraperitoneal infection with GT-1 tachyzoites while all laboratory mice (NMRI strain) died within 15 days. In vitro, tachyzoite proliferation of avirulent strains in mouse cells is inhibited by induction of IRG proteins with IFNγ (Könen-Waisman and Howard, 2007). In contrast, proliferation of type I virulent strains is not inhibited (Zhao et al., 2009c). By this assay, IFNγ-induced CIM diaphragm-derived cells (DDC see ‘Materials and methods’) inhibited proliferation of type I virulent RH-YFP strain tachyzoites as efficiently as they inhibited the proliferation of avirulent strains, while BL/6 DDC inhibited only the avirulent strains (Figure 4B). Cells from two other M. m. castaneus strains, CAST/EiJ and CTP were almost as resistant as CIM. Additionally, IFNγ-induced CIM cells died by reactive cell death after infection with both virulent RH-YFP and avirulent ME49, while BL/6 cells died only after infection with avirulent strains (Figure 4C). Reactive death of T. gondii-infected mouse cells after induction with IFNγ is associated with IRG protein-mediated host resistance, as previously reported (Zhao et al., 2009b).

Resistance of wild-derived mouse strains to virulent T. gondii.

(A) Cumulative mortality of NMRI and CIM mice infected with 100 or 300 (data pooled) tachyzoites of the indicated T. gondii strains. (B) IFNγ-mediated growth inhibition of virulent (type I RH-YFP, BK) and avirulent (type II ME49, type III CTG) T. gondii strains in DDC of laboratory (BL/6) and wild-derived, inbred mice (CAST/Ei, CIM, CTP). Proliferation of parasites was measured by 3H-uracil incorporation and is displayed as percentage of residual T. gondii proliferation, as described in ‘Materials and methods’. Error bars show standard deviations of quadruplicate values. (C) IFNγ-dependent reactive cell death of mouse DDC cell lines infected with T. gondii. DDC were either stimulated with 100 U/ml of IFNγ 24 hr prior to infection or left unstimulated. Cells were infected with type II strain ME49 or type I strain RH-YFP at the indicated MOIs for 8 hr. Cell viabilities were measured as described in ‘Materials and methods’ and expressed as percentages of those recorded for uninfected cells (MOI = 0). Error bars show standard deviations of quadruplicate values.

https://doi.org/10.7554/eLife.01298.018

Resistance of CIM mice to the type I virulent RH-YFP strain in vivo was exploited to test linkage of resistance to the IRG system in a (CIM×BL/6)F1×BL/6 backcross. For typing purposes the IRG gene clusters from CIM and BL/6 were differentiated by RFLPs in Irga1 (Chr 18) and Irgb6 (Chr 11). Fluorescent-tagged tachyzoites (RH-YFP) were injected intraperitoneally into mice and the frequency of infected peritoneal cells measured by FACS after 5 days. In five independent experiments involving a total of 65 typed animals (Figure 5A), resistance by this assay was almost completely dominant and was tightly linked to the IRGCIM gene cluster on Chr 11 (p<<10−6). There was no detectable association of virulence with the IRGCIM cluster on Chr 18. In a similar analysis of 53 F2 progeny, mice typed as homozygous IRGCIM at the Chr 11 gene cluster were as resistant as wild-type CIM mice. Thus the reduced resistance of some mice heterozygous for IRGCIM on Chr 11 may be due to a gene dosage effect at the IRG locus. Assayed directly by survival, 51 backcross and F2 mice infected with RH-YFP followed the same pattern as in the peritoneal cell assay (Figure 5B), with the Chr 11 IRGCIM homozygotes (cc in Figure 5B) showing complete resistance, the heterozygotes (bc) substantial but incomplete resistance and the IRGBL/6 homozygotes (bb) complete and acute susceptibility.

Resistance of CIM mice to virulent T. gondii is dependent on the Chr 11 IRG locus.

(A) Infected CD45+ peritoneal cells in (BL/6×CIM)F1×BL/6 backcross (5 experiments, total 83 mice) and (BL/6×CIM)F2 mice (4 experiments, total 69 mice) 5 days after i.p. injection of 500 RH-YFP tachyzoites. Genotypes at IRG loci and at the Nalp1 locus for backcross and F2 mice are shown (see key) as bb (homozygous BL/6), bc (heterozygous BL/6/CIM) or cc (homozygous CIM). Elimination of infected cells is linked to the CIM haplotype on Chr 11 (n.b. the y-axis is logarithmic below 5%). (B) Cumulative mortality of (BL/6×CIM)F1×BL/6 and (BL/6×CIM)F2 mice infected with 500 RH-YFP tachyzoites. Irgb6 genotypes are shown as in (A). (C) Cysts of type I T. gondii strains in brain homogenates of CIM mice infected 6–8 weeks earlier (quantitation in Table 1). Bar = 20 µm.

https://doi.org/10.7554/eLife.01298.019

Recent results have implicated the inflammasome core component Nlrp1 (NLR family, pyrin domain containing 1) in resistance to T. gondii in human (Witola et al., 2011) and rat (Sergent et al., 2005; Cavailles et al., 2006). Since the Nlrp1 complex locus is about 20 Mb telomeric to the IRG system on Chr 11 it was possible that polymorphism at this locus (Boyden and Dietrich, 2006) was responsible for the differential resistance apparently linked to the IRG complex. We therefore typed all the backcross progeny shown in Figure 5A by PCR designed to distinguish the BL/6 and CIM allotypes of the Nlrp1b locus (see ‘Materials and methods’). We obtained 17% recombinants between Irgb6 and Nlrp1. There was no correlation between Nlrp1 genotype and the ability to clear virulent T. gondii (Figure 5A). It is anyway unlikely that Nlrp1 plays a role in the resistance polymorphism we describe since it is not detectably expressed in the IFNγ-induced DDC transcriptomes (data not shown).

The existence of mouse genotypes resistant to virulent T. gondii strains is consistent with a co-evolutionary explanation for the evolution of virulence. To sustain the argument, however, it would be necessary to show that T. gondii strains that are lethal in laboratory mice, and thereby suffer a major cost, can form functional cysts in resistant CIM mice, permitting their propagation. We therefore searched for brain cysts in CIM mice infected 6–8 weeks earlier with virulent type I or avirulent type III strains of T. gondii. Two type I virulent strains, GT-1 and BK, formed cysts in CIM mice (Figure 5C, Table 1). Thus the resistance of the CIM mouse provides an adaptive niche for highly virulent T. gondii strains. The avirulent type III strain, NED, encysted in the laboratory mice >20× more efficiently than in CIM mice (Table 1).

Table 1

Cyst counts in T. gondii infected mice

https://doi.org/10.7554/eLife.01298.020
MouseUIC*CIMCIMCIMCIMCIMCIMCIMNMRINMRINMRINMRI
T. gondii (# injected)GT-1 500GT-1 1000BK 5000BK 10,000NED 10,000NED 10,000NED 10,000GT-1 500BK 500NED 10,000NED 10,000
Q-PCR (cycle)>3522.924.218.722.026.926.228.8DeadDead22.620.3
Cysts per brain01005013015022090157204800
Antibody test+++++++++
  1. *

    Uninfected control.

  2. Mice were sacrificed 5 weeks (NED) or 6–8 weeks (BK and GT-1) after tachyzoite injection. Infection was verified by serum antibody. Cysts were evaluated by direct counting in homogenised brains and by quantitative PCR of a repeat element of T. gondii (Reischl et al., 2003) in genomic DNA samples isolated from mouse brains.

The IRG resistance mechanism operates cell-autonomously, and in BL/6 cells the loading of IRG proteins onto the parasitophorous vacuole occurs only with avirulent strains even in cells infected simultaneously with virulent and avirulent strains (Zhao et al., 2009a; Khaminets et al., 2010). Consistently, the resistance of the CIM strain against virulent T. gondii was reflected in the behaviour of IRG proteins in IFNγ-induced CIM-derived cells infected with virulent RH-YFP strain T. gondii. In BL/6 cells Irgb6BL/6 loaded onto only about 8% of vacuoles, while in CIM cells the highly divergent Irgb6CIM protein loaded onto more than 50% of vacuoles (Figure 6A). Even more extreme, the tandem protein, Irgb2-b1BL/6, which is poorly expressed in BL/6 cells (Figure 6B), was not detectable on the RH-YFP PVM, while the highly divergent Irgb2-b1CIM was well-expressed in CIM cells and loaded onto over 95% of vacuoles (Figure 6A).

Irgb2-b1CIM protects other IRG members from inactivation.

(A) Immunofluorescent quantitation of loading of Irgb6 and Irgb2-b1 on to RH-YFP vacuoles in IFNγ-induced BL/6 and CIM DDC. (B) Strain dependence of expression levels of Irgb-tandem proteins. (C) Reduced phosphorylation of Irga6CIM on T108 by RH-YFP in IFNγ-induced CIM DDC (chopped western blot for calnexin, phosphorylated Irga6 and total Irga6). n.b. Irga6CIM characteristically runs at a higher apparent molecular weight than Irga6BL/6 (D) Transfected Irga6CIM and Irga6BL/6 are both phosphorylated in IFNγ-induced L929 cells infected with RH-YFP as shown in western blot of detergent lysates. Phosphorylation of Irga6 is indicated by a size-shift (black arrowhead) for both Irga6BL/6 and Irga6CIM in infected cells. The lower band in the two transfected/infected tracks is the endogenous Irga6BL/6. (E) Irgb2-b1CIM (yellow) transfected into IFNγ-induced BL/6 MEFs inhibits phosphorylation of Irga6 (red) by RH-YFP seen in untransfected cells serving as control. Bar = 10 µm. (F) Immunofluorescent quantitation of phosphorylated Irga6 on the PVM of RH-YFP in Irgb2-b1CIM-transfected cells and untransfected cells prepared in (E). (G) Immunofluorescent quantitation of total Irga6 on the PVM of RH-YFP in Irgb2-b1CIM transfected and untransfected cells prepared in (E). (H) Enumeration of Irgb6-positive vacuoles in BL/6 MEFs induced by IFNγ and transfected with Irgb2-b1CIM or Irgb2-b1BL/6.

https://doi.org/10.7554/eLife.01298.021

The loading of the effector IRG protein Irga6 onto the parasitophorous vacuole of virulent T. gondii strains is prevented by a parasite-derived kinase complex (ROP5/ROP18) that phosphorylates two threonines that are essential for IRG protein function (Steinfeldt et al., 2010). Remarkably, in CIM cells infected with RH-YFP, phosphorylated Irga6CIM was barely detectable with an antiserum specific for Irga6BL/6 phosphorylated at T108 (Figure 6C). Irga6CIM differs from Irga6BL/6 at only two residues, both distant from the phosphorylation sites and was phosphorylated normally when transfected into IFNγ-induced L929 cells infected with virulent RH-YFP (Figure 6D). Thus Irga6CIM apparently remains unphosphorylated in CIM cells as a result of active inhibition of the parasite kinase complex. The following experiment showed that the highly polymorphic tandem protein, Irgb2-b1CIM, is largely responsible. Irgb2-b1CIM was transfected into IFNγ-induced BL/6 mouse embryonic fibroblasts (MEFs) infected with RH-YFP virulent strain T. gondii. Phosphorylated Irga6 was measured at the PVs in transfected cells expressing Irgb2-b1CIM and in untransfected cells. Figure 6E,F show that the amount of phosphorylated Irga6 was strikingly reduced on vacuoles loaded with Irgb2-b1CIM while the amount of total Irga6 loaded onto Irgb2-b1CIM positive vacuoles was increased (Figure 6G). Thus the decreased signal of phosphorylated Irga6 on the PVM was not caused by competition for loading between Irga6 and Irgb2-b1, but rather by inhibition of phosphorylation. Transfected Irgb2-b1CIM also stimulated the loading of endogenous Irgb6BL/6 onto RH-YFP vacuoles (Figure 6H). Transfected Irgb2-b1BL/6, which is well expressed unlike the endogenous protein, had little or no effect on the loading of Irgb6BL/6 (Figure 6H). Thus the essential difference between Irgb2-b1BL/6 and Irgb2-b1CIM in determining resistance lies in the amino acid sequence polymorphism rather than in the protein expression level.

Preliminary results suggest that Irgb2-b1CIM may bind directly to the protein product of the virulent allele of ROP5. Thus loading of Irgb2-b1CIM onto the PVM of ROP5-deficient RH strain parasites in CIM cells was greatly reduced, and consistently, the amount of loading on to different RH-related strains correlated with strain-specific variation in the amount of ROP5 (Figure 7A). Irgb2-b1CIM itself becomes phosphorylated during infection with virulent T. gondii (Figure 7B), thus is also a target for the active ROP5-ROP18 kinase complex, suggesting that Irgb2-b1CIM may block phosphorylation of Irga6 by binding ROP5 pseudokinase at the vacuole, thereby distracting rather than inhibiting ROP18 kinase. ROP5 binds to Irga6 via helix 4 (H4) of the Irga6 nucleotide binding domain (Fleckenstein et al., 2012), so a homologous structure on Irgb2-b1 may be involved in ROP5 interaction. Indeed the putative H4 and αD structural domains of the Irgb2 subunit are highly polymorphic and show recent divergent selection (Figure 7C), indicating possible co-evolution with ROP kinases and pseudokinases. The high polymorphism of H4 in Irgb2-b1 is also consistent with a direct interaction with a polymorphic component of the pathogen. If Irgb2-b1 interacts with a host protein to bridge to ROP5 the interaction surface between the two host proteins would not be expected to evolve rapidly under divergent selection.

IRG-tandem proteins interact with virulence factors.

(A) Loading of Irgb2-b1CIM on to vacuoles of RH variants in IFNγ-induced CIM DDC. Irgb2-b1CIM loading (dot plots, upper image) is positively correlated with ROP5 expression level in the parasite (western blot, lower image). (B) Autoradiogram (33P) of immunoprecipitated IRG proteins in DDC infected with T. gondii. Irga6 was phosphorylated (open arrow head) by virulence factors of T. gondii in BL/6 DDC, but not in CIM DDC. Irgb-tandem proteins were phosphorylated only in CIM DDC (filled arrow head) in a ROP5/ROP18-dependent manner. UIC = uninfected control. Asterisk (*) indicates non-specific phosphorylated proteins (C) Ribbon model of Irgb2 predicted based on the structure of Irga6, showing diversifying selection associated with H4 and αD of the G-domain. The colours of the ribbons are based on the π and πa/πs values among 40 alleles sequenced from mouse strains and wild mice with a 120 bp slide window and 10 bp step. The colours indicate the πa/πs value, from purifying selection (blue) to significantly diversifying selection (red). The saturations of colours are defined by overall π value in the sliding window, indicate conserved regions of the protein (low saturation) to highly polymorphic regions (high saturation).

https://doi.org/10.7554/eLife.01298.022

Discussion

We have shown that the IRG protein system essential for resistance against T. gondii infection in the mouse has a complex polymorphism on the scale of the MHC, and that at least one IRG haplotype, found in the wild-derived CIM strain mouse, is strikingly resistant to T. gondii strains that are highly virulent for laboratory mice. We also provide a mechanistic explanation for the resistance of the CIM mouse against type I virulent T. gondii strains. Resistance is determined by the presence of the polymorphic tandem IRG protein, Irgb2-b1CIM encoded on Chr 11, which blocks the ROP5/ROP18 kinase complex of the virulent parasite, preventing phosphorylation and consequent inactivation of IRG effector proteins. Taken together, our results suggest a selective explanation for the evolution of T. gondii strains that are highly virulent for certain mice. If the mouse is an evolutionarily significant host for T. gondii the parasite must balance its virulence against mouse resistance, in order to allow encystment. To minimise the cost of infection, selection on the mouse favours the evolution of strong resistance alleles. This in turn leads to the selection of parasite strains able to counteract heightened resistance sufficiently to allow encystment in these mouse genotypes, as type I T. gondii strains in CIM mice. However such virulent T. gondii strains are counterselected by acute lethality in less resistant mice, while less resistant mice are better hosts for less virulent T. gondii strains.

Why, however, are not all mice highly resistant? Loss of the entire IRG system in several vertebrate groups, for example higher primates and birds (Bekpen et al., 2005), suggests that possession of the IRG system may be costly. Highly resistant genotypes may be more costly than less resistant ones. Alternatively, molecular specificity in interactions between polymorphic parasite virulence factors and mouse IRG proteins may favour IRG genotypes that are highly resistant to some T. gondii genotypes but more susceptible to others. Such an allele-specific system is familiar from polymorphic plant disease resistance (R) genes and strain-specific pathogen virulence and avirulence (Flor 1971; Dodds et al., 2006), where exact molecular matches or mismatches determine the outcome of a given infection. These alternative models can now be analysed formally and tested experimentally.

Much of the argument in this paper has focused on the importance of the house mouse as an intermediate host and vector for T. gondii, resting on the global abundance of the species and its sympatry with the cat, and obviously supported by the intimate antagonistic relationship between T. gondii virulence factors and mouse resistance factors. However, although many bacteria and protozoal parasites are not resisted by the IRG system, T. gondii is not the only organism that is. At least two members of the Chlamydia species complex are resisted by the IRG system in mice, and polymorphic variation on Chr 11, possibly associated with Irgb10, affects the level of resistance (Bernstein-Hanley et al., 2006; Miyairi et al., 2007). There will surely be other organisms that may contribute to the genomic complexity and polymorphism of the IRG system. For example, the massive and ancient polymorphism of Irgb6 is not directly accounted for by our experiments. In in vitro experiments in BL/6 cells transfected Irgb2-b1CIM protects Irga6 from phosphorylation in the absence of the CIM allotypes of the other IRG proteins including Irgb6 (Figure 6) and in the CAST/Ei strain, which is almost as resistant as CIM against T. gondii, Irgb6 is barely expressed (unpublished results). It may be that Irgb6 polymorphism reflects a pattern of resistance against another abundant mouse pathogen. However it is also not excluded that Irgb6 polymorphisms relate to further virulence polymorphism in T. gondii not expressed in the limited range of strains used in the present study. Likewise, mice are not the only intermediate hosts for T. gondii. The parasite infects all known mammals and birds, and many other species than M. musculus are prey for domestic cats. Furthermore, individuals of other species, for example rats (Jacobs and Jones, 1950) and American deer mice (Peromyscus) (Frenkel, 1953) have been shown to resist infection with strains virulent for laboratory mice. Thus some of the genomic complexity of the ROP system and other virulence-associated T. gondii secretory proteins may be relevant to immunity modification in other host species. Nevertheless, despite these distractions, mice and T. gondii have clearly had a large selective impact on each other.

The close association of cat and mouse with humans has led to large-scale transformations in the ecology of the parasite over the last 10,000 years. The impact of the dramatic narrowing of the parasite’s effective host range on the genetics of the virulence-resistance relationship may help to understand better the co-evolutionary forces at work in this system. The patterns of genetic resistance against T. gondii in its most important intermediate hosts will determine the relative abundance of strains contaminating the environment, and thereby the strains available for human infection.

Materials and methods

Nomenclature of IRG genes and proteins

The systematic, phylogenetically-based nomenclature of IRG genes and proteins used in this paper was published in Bekpen et al. (2005) and replaced the non-systematic naming of some of the first-discovered members of the family. A partial synonymy relating the new nomenclature to other names that have appeared in the literature is given in Martens and Howard (2006). The root name for members of the IRG family is IRG. This may followed by single letters designating phylogenetically distinct sequence sub-families, as IRGA, IRGB, IRGC, IRGD, IRGM. Individual gene or protein names are given in the standard mouse nomenclatural form, as Irga6 (protein) and Irga6 (gene) or Irgb6 (protein) and Irgb6 (gene). A subset of IRGB genes consist of two adjacent IRG coding units that are transcribed together from single promoters and spliced to form ‘tandem’ IRG proteins with a molecular weight twice that of the individual IRG coding units. In the original study it was not clear whether the individual coding units could also be expressed separately and they were therefore given separate names (as Irgb1 and Irgb2) (Bekpen et al., 2005). It is now clear that these coding units are expressed only as tandems. In the present study we have therefore named the tandem genes and proteins with the names of their constituent coding units in N-C order, thus Irgb2-b1 is a tandem IRG protein with the Irgb2 coding unit N-terminal to the Irgb1 coding unit.

Sequencing and assembly of IRG genes of inbred mouse strains and wild mice

The Mus musculus genomes sequenced by the Mouse Genomes Project (MGP) (Keane et al., 2011), including 129P2/OlaHsd, 129S1/SvImJ, 129S5/SvEvBrd, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/N, LP/J, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, WSB/EiJ and the Mus spretus genome, SPRET/EiJ, were accessed by LookSeq (http://www.sanger.ac.uk/resources/mouse/genomes/), and the sequences of IRG genes were assembled (for details see below, ‘Bioinformatics’). Three 129 strains (129P2/OlaHsd, 129S1/SvImJ and 129S5/SvEvBrd) were identical in all IRG coding units, so they were combined into one virtual strain, 129. Mouse strain C57BL/6NJ was confirmed identical for all IRG genes with C57BL/6J, so the results were combined into one strain, C57BL/6. The sequences of IRG genes of mouse strains Czech II, NMRI and JYG were acquired from the NCBI database and are listed in Table 2. The CAST/Ei BAC library CHORI-26 was screened with 40bp synthetic probes for Irga1, Irga3, Irga4, Irga6, Irgm1, Irgm2, Irgb1, Irgb6, Irgb8 and Irgb10. Eight out of 61 positive BAC clones were sent to the Wellcome Trust Sanger Institute for shotgun sequencing as follows: 226N16 (NCBI accession number CU695224), 243M20 (CU695226), 445J9 (CU695230), 332A4 (CU695228), 333C17 (CU695229), 240F21 (CU695225), 316B17 (CU695227) and 76B7 (CU695231). The sequences of IRG genes were extracted from these results and cross-checked with the data from the MGP. Clones containing IRG genes from the mouse strain MSM/Ms (Abe et al., 2004) were chosen based on the BAC end sequences from RIKEN BRC (http://www.brc.riken.jp/lab/dna/en/MSMBACen.html). Seven clones were sent to the Beijing Genomics Institute (BGI) for Illumina sequencing: 329H21, 362F04, 544P17, 494M12, 419B05, 148E20 and 355D01. The sequencing results were assembled and uploaded to the NCBI GenBank with accession number KF705682, KF705684, KF705686, KF705680, KF705685, KF705681, KF705683. IRG genes of the CIM mouse strain were sequenced via full transcriptome Illumina sequencing of IFNγ-induced diaphragm-derived cells (DDC, see below) in the Cologne Centre of Genomics (CCG). For wild mouse samples, genomic DNA was acquired from a variety of sources. From this material 5 key IRG members were amplified by PCR with appropriate primers (Table 3). The full ORFs of Irgm1, Irgb2, Irgb6 and Irgb10, and a partial sequence of Irga6 (964 bp in length) were amplified. PCR products were cloned into the pGEM-T vector and insertions in individual positive clones were sequenced. The sequences are attached as supplementary files in FASTA format.

Table 2

IRG sequences from NCBI database

https://doi.org/10.7554/eLife.01298.023
GeneStrainTypeAccess number
Irgm1Czech IIESTsBI150356, BF161711, BF168437, BF164781, BE367794
Irgb2-b1Czech IImRNABC022776
JYGmRNAAK145236
Irgb6Czech IImRNABC093522
Czech IImRNABC034256
JYGmRNAAK166353
NMRImRNABC085259
C.D2mRNAU15636
IrgdCzech IImRNABC001986, BC009131
Irgm2Czech IIESTsBG518498, BF137080, BE284209, BF168033, BI149246, BI414397, BE283352, BE306442
Irgm3Czech IIESTsBI414397, BI149246, BF163420, BE283352, BF168033, BI153387, BE281683, BE306442, BI106672, BI150745, BF225799, BF168273
Irga6Czech IIESTsBF143764, BI150692, BF163277, BE369870, BI152144, BF168743, BF022265, BI105027, BE306549, BF140175
NMRIESTsBG862486, BI654967, BI854263, BI654186, BG974278, BI662561, BI853679, BG864306, BI658908
Irga8Czech IImRNABC023105
Irga9Czech IImRNABC040796
Irga10Czech IImRNABC020118
Table 3

Primer list

https://doi.org/10.7554/eLife.01298.024
NameSequence 5′ to 3′Function
Irga6_56B_fwCTACTATGAATGGTATATGTAGCATTGTGIrga6 amplification
Irga6_56B_bwCAGGACTTCAGCTTAATTAGAAGGCIrga6 amplification
Irgb2_66F_fCTGGACTCTGCGCTTTTATTGGIrgb2 amplification
Irgb2_66F_bCTGGAAACACTTTGCCCACGIrgb2 amplification
Irgb6_67Y_fwCCTCTCTTCTCCATTCAGCTTCIrgb6 amplification
Irgb6_67Y_bwCCAAGGTGAAGCTAAGAGTGAACIrgb6 amplification
Irgb10_682_fwCTCCAGTGTCCTGTGTGCCCIrgb10 amplification
Irgb10_682_bwCAGGAATGCCCTCAGTCGTCIrgb10 amplification
Irgm1_655_fwCTGCCGATTCGATTCATAAACIrgm1 amplification
Irgm1_655_bwCCTCTCAGAGAATCTAAAACCCIrgm1 amplification
Irgm1_66F_bwGAGACAGGGGAGATGAGTGATIrgm1 amplification
Irga1_221_fwATCGATAGTTCCCTTGTCAATGTGGbackcross and F2 mice genotyping, Chr 18
Irga1_221_bwTTTGTAGAGTTTGGCTAGGGCCTGbackcross and F2 mice genotyping, Chr 18
Irgb6_21D_fwATGGCTTGGGCCTCCAGCTTbackcross and F2 mice genotyping, Chr 11
Irgb6_614_bwCCACCATTCCACTTGGTGGbackcross and F2 mice genotyping, Chr 11
Tox-9AGGAGAGATATCAGGACTGTAGT. gondii qPCR primer
Tox-11GCGTCGTCTCGTCTAGATCGT. gondii qPCR primer
Nlrp1_FWAACTTATCTCAGGTCTCTGTGATTNlrp1b genotyping, forward
Nlrp1_BL6GATATAGGTCAGGACCAATGCNlrp1b backward, BL/6 specific
Nlrp1_CIMGATATAGGTCAGGACCATCAANlrp1b backward, CIM specific

Bioinformatics and sequence analysis

Neighbor-joining trees of IRG genes and proteins were built with MEGA5 (Tamura et al., 2011). The MSM/Ms BAC Illumina data were assembled de novo with the assistance of Geneious Pro 5.5.6 (Biomatters Ltd.). Dot plots of genomic IRG gene clusters of BL/6 vs CIM and BL/6 vs MSM/Ms were calculated by LBDOT 1.0 (Lynnon Corporation) with a sliding window of 20 bp and a maximum mismatch of 2 bp. The raw Illumina reads from inbred mouse strains were accessed by LookSeq and all reads were grouped based on SNPs and manually aligned to their BL/6 homologues. Individual IRG gene sequences are available through Genbank. For analysis of the average diversity between house mouse strains, the sequences of 50 random functional genes were acquired from MGP and the National Institute of Genetics (NIG), Japan (http://molossinus.lab.nig.ac.jp/msmdb/). The analysis covered seven mouse strains: two laboratory inbred strains, BL/6 (MHC haplotype b) and AKR/J (MHC haplotype k); four wild-derived inbred house mouse strains, MSM/Ms, CAST/EiJ, PWK/PhJ and WSB/EiJ; the M. spretus strain Spretus/EiJ. As shown in Figure 3—source data 1, genes were arbitrarily selected based on numerical position in the NCBI reference assembly build 37. Potentially functional genes closest to the designated genome position with EST evidence on the NCBI database were chosen. If the ORF of the gene was longer than 1500 bp, only 1500 bp were considered. Tajima’s π and πa/πs values were calculated with DnaSP5 (Librado and Rozas, 2009). The secondary structure of Irgb2-b1 was predicted by PSIpred (UCL department of computer science, http://bioinf.cs.ucl.ac.uk/psipred/).

Culture of T. gondii strains

T. gondii strains (Table 4) were maintained by serial passage in confluent monolayers of Hs27 cells. When Hs27 cells were lysed by T. gondii tachyzoites, parasites were harvested from the supernatant and purified from host cell debris by differential centrifugation (5 min at 100×g, 15 min at 500×g). The pelleted parasites were resuspended in IMDM, 5% FCS supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin (PAA, Pasching, Austria), counted and immediately used for infection of mice, cells or lysed for subsequent immunoblot.

Table 4

T. gondii strains used in this study

https://doi.org/10.7554/eLife.01298.025
TypeStrain nameReferenceNote
I (virulent)RH(Albert and Sabin, 1941)
RH-YFP(Gubbels et al., 2003)transgenic RH strain expressing YFP
RHΔrop5(Behnke et al., 2011)transgenic RH strain, the ROP5 locus has been deleted
RHΔrop18(Reese et al., 2011)transgenic RH strain, the ROP18 locus has been deleted
BK(Winsser et al., 1948)
GT-1(Dubey 1980)canonical type I strain, full sequence in ToxoDB Database
II (avirulent)ME49(Lunde and Jacobs, 1983)
III (avirulent)NED(Darde et al., 1992)

Rationale for assay of specific IRG proteins in these experiments

The IRG proteins all have distinctive properties. Irga6 and Irgb6 are the most highly expressed (in lab mice) and there are excellent serological reagents available for them. We therefore used Irga6 and Irgb6 for experiments that monitor loading at the vacuole. In addition, we have antisera against the specific phosphoserines on Irga6 so we can measure phosphorylation directly. Irgb6 is however more drastically affected by the genetic difference between avirulent types II and III T. gondii strains and virulent type I strains, dropping from up to 90% loading of vacuoles to around 10%. Irga6 is also greatly affected but the effect is seen more conspicuously as a reduction in the intensity of loading rather than in a reduction in the percent of vacuoles detectably loaded. These and other distinctive properties, are described elsewhere (Khaminets et al., 2010; Steinfeldt et al., 2010; Fleckenstein et al., 2012). The tandem IRG protein, Irgb2-b1, became a focus of attention because of its high expression in resistant CIM mice, its large polymorphic variation, and evidence for being under recent divergent selection (see below).

Preparation of tissue culture lines from mouse diaphragm cells

The origins of mice used in this study are listed in Table 5. Cells were prepared from diaphragm tissue by a modification of the technique described by Antony et al., (1989). Diaphragm-derived cells are easy to prepare and have the advantage over MEFs that they can be prepared from a single adult mouse, enabling individual genetically different animals to be studied genetically and functionally at the cellular level. One mouse from each of BL/6, CAST/EiJ, CTP and CIM strains was sacrificed and the diaphragm removed under sterile conditions. The diaphragm was washed with PBS, chopped up, incubated with collagenase/dispase (1 mg/ml, Roche, Mannheim, Germany) for 1 hr at 37°C and then centrifuged for 15 s at 100×g. The supernatant was collected, centrifuged for 5 min at 500×g and the pellet plated in DMEM, 10% FCS supplemented with 4 mM L-glutamine, 2 mM non-essential amino acids, 1 mM sodium pyruvate, 1× MEM non-essential amino acids, 100 U/ml penicillin, 100 μg/ml streptomycin (all PAA, Pasching, Austria). The remaining cell debris after collagenase/dispase-incubation was further incubated in 1× trypsin (Gibco, Grand Island, New York, USA) for 1 hr at 37°C, and then centrifuged for 15 s at 100×g. The supernatant was collected, centrifuged for 5 min at 500×g and the pellet plated (see above). Primary diaphragm-derived cells (DDC) were grown until they had reached ∼50% confluence and then transfected with 2 µg of psv3-neo (Southern and Berg, 1982) using the FuGENE HD transfection reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol. Cells were put under selection with G418 (Geneticin, PAA, Pasching, Austria) at a concentration of 150 μg/ml until immortalised clones had overgrown the culture. DDC isolated from mice and the mouse cell line L929 (from mouse strain C3H) were maintained in supplemented DMEM (see above) without G418.

Table 5

Origin of mouse samples and mouse genomic DNA

https://doi.org/10.7554/eLife.01298.026
Sample nameSubspeciesOriginLocation of collectionProvided by
D9, D18, D12, D22, D31, D34M. m. domesticusGermany50°50′N 6°45′EGenomic DNA provided by B Harr Max Planck Institute for Evolutionary Biology, Germany
MC8, MC4, MC6, MC52, MC13, MC27, MC58M. m. domesticusFrance44°20′N 3°0′E
W1.1, W3.1, W3.2, W4.1, W7.1M. m. musculusAustria48°12′N 16°22′E
AL12, AL21, AL24, AL30, AL32, AL41M. m. musculusKazakhstan43°N 77°E
MW2, MW4M. m. musculusInner Mongolia China41°5′N 108°9′ECaught by J Lilue for this study. Institute for Genetics, University of Cologne, Germany
MT1, MT240°47′N 111°1′E
JH4, JH6, JH11, JH12M. m. musculus or Hybrid zoneHebei Province China37°37′N 115°19′E
YX3, YX5, YX11M. m. castaneusHenan Province China32°4′N 115°3′E
MIB3, MIB4, MIB6,M. m. castaneusIndia13°3′N 77°34′ECaught by UB Müller for this study. Institute for Genetics, University of Cologne, Germany
MIB23, MIB24, MIB2513°6′N 77°34′E
MIB35, MIB3612°54′N 77°29′E
CTP (living mice)M. m. castaneusThailandMouse strainF Bonhomme, Institut de Science de l’Evolution, Montpellier, France
CIM (living mice)M. m. castaneusIndia
CAST/Ei (living mice)M. m. castaneusThailandInbred strainThe Jackson Laboratory, Bar Harbor, Maine, USA
C57BL/6 (living mice)M. m. domesticusLab mouseInbred strainCentre for Mouse Genetics, University of Cologne, Germany
NMRI (living mice)M. m. domesticusLab mouseInbred strainCharles River Laboratories, Sulzfeld, Germany

Cell induction, transfection and infection

Cells were induced for 24 hr with 200 U/ml of IFNγ (Peprotech, Rocky Hill, New York, USA) unless indicated otherwise. Irga6BL/6 and Irga6CIM were cloned into the pGW1H vector with C-terminal ctag1 tags (Martens et al., 2005); full length Irgb2-b1BL/6 and Irgb2-b1CIM were cloned into the pGW1H vector with C-terminal Flag tags. Constructs were transfected using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol. The multiplicities of infection (MOI) were 1 for the 3H-uracil incorporation assay, 2–5 for immunofluorescence microscopy, 2.5, 5 and 10 for the cell viability assay, and ∼10 for in-cell phosphorylation experiments. Cells were either fixed for immunofluorescence or lysed for western blot 2 hr after infection.

Immunofluorescence microscopy and analysis

Cells were fixed with PBS/3% paraformaldehyde (PFA) for 20 min at room temperature (RT), washed three times with PBS and then permeabilized with 100% methanol on ice (for stainings including serum 87,558, below) or PBS/0.1% saponin at RT (all other antibodies) for 10 min followed by blocking with PBS/3% bovine serum albumin (BSA) for 1 hr. Cells were incubated with primary antibodies diluted in PBS/3% BSA for 1 hr and subsequently incubated with secondary antibodies for 30 min at RT. Antibodies against Irgb6 (141/1) and against the conserved Irgb-tandem C-terminal peptide CLSDLPEYWETGMEL (954/1-C15A) shared by Irgb-tandem proteins of both BL/6 and CIM mice raised at Innovagen AB (Lund, Sweden). Rabbit polyclonal anti-Irga6 phosphorylated at T108 has been described (serum 87,558) (Steinfeldt et al., 2010). Other primary immunoreagents were anti-recombinant Irga6 antiserum 165/3 (Martens et al., 2004), mouse anti-FLAG (M2, Sigma-Aldrich, St. Louis, Missouri, USA) and rabbit anti-calnexin (Calbiochem, Darmstadt, Germany). Second-stage antibodies were: Alexa 488 and Alexa 555 labelled donkey anti-mouse and anti-rabbit sera (Molecular Probes, Eugene, Oregon, USA). Images were taken with a Zeiss Axioplan II fluorescence microscope equipped with an AxioCam MRm camera (Zeiss, Jena, Germany). Images were processed with Axiovision 4.7 (Zeiss, Jena, Germany). Quantification of IRG protein signal intensity at the T. gondii PVM was performed as described before (Khaminets et al., 2010). All quantification of microscopical images was performed double blind. Error bars in Figure 6A,G represent standard deviations of repeated measurements.

Western blot analysis

4×105 cells were seeded to individual wells of a six-well plate and induced with IFNγ for 24 hr. Cell lysis and western blot analysis was performed essentially as described elsewhere (Steinfeldt et al., 2010).

Immunoprecipitation

6×105 BL/6 and CIM DDC were seeded in 6-cm dishes and induced with IFNγ for 24 hr. Metabolic labelling with 33P-phosphoric acid (Hartmann Analytic, Braunschweig, Germany), cell lysis and immunoprecipitation was performed essentially as described elsewhere (Steinfeldt et al., 2010).

In vitro 3H-uracil incorporation assay for measuring T. gondii proliferation

T. gondii proliferation was measured using the 3H-uracil incorporation assay (Pfefferkorn and Guyre, 1984). DDC were seeded on 96-well plates (6500 cells/well) and induced with IFNγ (100 U/ml) or left untreated. 24 hr after induction cells were infected for a further 24 hr with specified T. gondii strains at different multiplicities of infection, or left uninfected. The cultures were labeled with 0.3 μCi/well of 3H-uracil (3HU, Hartmann Analytic, Braunschweig, Germany) for 24 hr and then frozen at −20°C. The amount of radioactivity incorporated into proliferating parasites was determined by a MatrixTM 9600 β-counter (Packard, Meriden, Connecticut, USA). Data are shown for MOI = 1 and presented as the percentage of residual parasite proliferation under IFNγ treatment (Figure 4B). Residual parasite proliferation was defined as follows: 100—([3HU counts—background in infected, IFNγ-treated culture/mean 3HU counts—background in infected, non-treated cultures] ×100) where background is 3HU (mean) counts of uninfected, non-induced cultures.

Cell viability assay

DDC were seeded and induced as described for the 3H-uracil incorporation assay (see above). Cells were infected with type I RH-YFP or type II ME49 strains of T. gondii with indicated MOIs for 8 hr. Viable cells were quantified by the CellTiter 96 AQueous non-radioactive cell proliferation assay (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol. The absorption of a bioreduced formazan of the tetrazolium compound MTS, which is generated by metabolically active cells during incubation at 37°C for 2–4 hr, was measured in an ELISA reader (Molecular Devices, Menlo Park, California, USA) at 490 nm. The quantity of formazan product is proportional to the number of living cells in the culture.

In vivo survival assay and genotyping

Mice were infected i.p. with 500 RH-YFP tachyzoites in 200 µl of PBS and tail samples were taken when animals succumbed during the acute phase of infection. Survivors were sacrificed 60 days post infection, tested for sero-conversion using the Toxocell Latex Kit (biokit, Barcelona, Spain) and tail biopsies taken. Biopsies were digested in 500 µl of buffer (100 mM Tris-HCl [pH 8.5], 5 mM EDTA, 200 mM NaCl, 0.2% SDS, 150 µg/ml proteinase K) and genomic DNA precipitated with isopropanol. An 804 bp fragment of Irga1 and an 857 bp fragment of Irgb6 were amplified from genomic DNA using the primers listed in Table 3. PCR products were digested with restriction enzymes AccI (Irga1) or FokI (Irga6, both New England BioLabs, Ipswich, Massachusetts, USA) for 45 min at 37°C, followed by 20 min at 60°C. DNA fragments were separated on a 2% agarose gel. Nlrp1b fragments were amplified with a universal forward primer and strain-specific backward primers (BL/6 or CIM, see Table 3).

Flow cytometry to assay infected peritoneal cells

Mice were infected i.p. with 500 RH-YFP tachyzoites, sacrificed on day 5 post infection and subsequently subjected to peritoneal lavage with 6 ml of PBS. Lavage suspension (1 ml) was centrifuged in microtubes for 5 min at 500×g, the supernatant was discarded, the cell pellet resuspended with 30 µl of PBS/0.5% BSA containing PE-conjugated rat anti-mouse CD45 antibody (BD Biosciences, San Jose, California, USA) and subsequently incubated for 20 min on 4°C in the dark. The microtubes were then filled with PBS/0.5% BSA, centrifuged for 5 min at 500×g, the supernatant discarded and stained cells resuspended in 300 µl of PBS/0.5% BSA. Cells were analysed using a BD FACS Calibur flow cytometer (BD Biosciences, San Jose, California, USA) and the percentage of infected CD45-positive cells was calculated as percentage of events positive for YFP over 5×104 CD45-positive cells using WinMDI 2.9.

Quantitation of T. gondii in brains of infected mice

Mice were infected with T. gondii tachyzoites in 200 µl of PBS as indicated in Table 1. 6–8 weeks (GT-1 and BK) or 5 weeks (NED) post infection mice were sacrificed, the brains removed and triturated in 1 ml of PBS. Cysts were counted in 15–20 drops of 10 µl per brain homogenate to estimate the total number of cysts per brain. Additionally, homogenised mouse brains were digested with proteinase K (final concentration 100 μg/ml) overnight, and total genomic DNA was isolated with DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The presence of T. gondii DNA was detected with a Taqman qPCR method (7900fast; Applied Biosystems, Foster City, California, USA) by primer Tox-9 and Tox-11 as described before (Reischl et al., 2003).

Statistical analysis

Differences were tested for statistical significance using the unpaired two-tailed Student’s t test.

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Decision letter

  1. Detlef Weigel
    Reviewing Editor; Max Planck Institute for Developmental Biology, Germany

eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.

Thank you for sending your work entitled “Toxoplasma and the mouse: reciprocal virulence and resistance polymorphism” for consideration at eLife. Your article has been favorably peer reviewed by Detlef Weigel, eLife Deputy editor, and two other reviewers.

The Deputy editor and the other reviewers discussed their comments before we reached this decision, and the Deputy editor has assembled the following comments to help you prepare a revised submission.

This manuscript reports on the role of different mouse IRG genotypes in host defense against Toxoplasma gondii. The complexity and level of polymorphism in the IRG system are comparable to that at the MHC, and in a wider collection of mouse strains IRG genotypes can be found that confer resistance to strains of T. gondii that are highly virulent in laboratory mouse strains. In addition, the authors provide evidence that specific IRGs from resistant mouse strains function by dysregulating the activity of parasite virulence factors. The experiments are well designed and conducted, and the results are properly interpreted. The authors go on to speculate that the IRG polymorphism in mice (and loss of the entire IRG system in several vertebrate groups) reflects a fitness trade-off. In addition, the authors acknowledge that the mouse–T. gondii interaction is probably only a part of the evolutionary picture, and that IRG evolution is likely to be driven by other pathogens as well, such as Chlamydia.

The reviewers, however, felt that some of the evolutionary inferences are overstated. For example, the abstract begins with “Virulence in T. gondii for its natural intermediate hosts, the mouse, is an evolutionary paradox…” This would only be the case if one takes laboratory mice, known to be highly derived, as representative of wild mice. In fact, prior studies have shown that deer mice (Peromyscus) are resistant to virulent strains of T. gondii (Am. J. Trop. Med. Hyg. 2, 390-415 (1953), as is the case for other common hosts such as chickens and rats. It is therefore reasonable to assume that wild house mice might express resistance mechanisms not found in laboratory mice, given the extremely narrow genetic diversity of the latter. The authors find such a mechanism and this is certainly interesting, but perhaps not as surprising as claimed. Hence, one could conclude that the virulence mechanisms of the parasite have evolved for “resistant hosts” in general and not wild mice per se. Although the system seems to resemble the R gene-avirulence gene systems for plants and their pathogens, the co-evolution arguments should be toned down considerably, as the work stands on its own.

Major comments:

1) There is a concern about the mechanism proposed for Irgb2-b1 function. In cells from CIM mice infected with RH parasites, the authors demonstrate a substantial increase in Irgb6 and Irgb2-b1 loading onto the parasite vacuole, compared to cells from B6 mice. In addition, although there is undetectable p-Irga6CIM in CIM cells infected with RH parasites, Irga6CIM can be phosphorylated if transfected into other cells (L929 cells), suggesting that Irga6 phosphorylation is actively inhibited in CIM cells. The authors state that Irgb2-b1CIM inhibits the phosphorylation of endogenous Irga6 in CIM mice. This statement is based on experiments in which they transfect Irgb2-b1CIM into B6 cells stimulated with IFN-γ and observe a reduction in p-Irga6 localization to the parasite vacuole membrane, which they quantify. However, they do not directly show that Irgb2-b1CIM inhibits Irga6 phosphorylation. It seems that an alternate interpretation of these data (from Figure 6E,F) is that Irgb2-b1CIM may inhibit the localization of Irga6 to the vacuole, where it is then phosphorylated. This point should be addressed/clarified.

Overall, the data implicating ROP5 in the loading of Irgb2-b1CIM onto the vacuole surrounding virulent parasites is very indirect. Granted it is decreased in Δrop5 parasites, but this does not equate to a model where Irgb2-b1 binds to ROP5 or inhibits its activity. Other indirect mechanisms are possible. This portion of the manuscript is too speculative. Either concrete data need to be provided or this should be extensively modified to indicate it might result from direct or indirect binding. Does Irgb2-b1 have a direct effect on ROP18? It would look the same phenotypically (Δrop5 parasites would phenocopy this). The model promoted here suggests that ROP5 binds to IRGs. If this interaction is robust, it should be straightforward to demonstrate this for Irgb2-b1. If such data are elusive, the authors should probably rethink their model. Figure 7 is somewhat speculative, and is also not well explained. For example, what is the significance of the IIa/IIs ratios in the graphic?

In the last paragraph of the Results when the authors describe Figure 7, they state, “Preliminary results suggest that Irgb2-b1CIM may bind directly to the protein product of the virulent allele of ROP5.” What are these preliminary results? Including this interaction data would significantly strengthen the authors’ model that Irgb2-b1CIM binding to ROP5 may contribute to blocking Irga6 phosphorylation in CIM mice.

2) The authors rely at least in part on Illumina resequencing data for their phylogenetic studies; such data are notoriously problematic for highly polymorphic and complex, duplicated regions of the genome. It would be appropriate for the authors to discuss how the BAC sequences they generated (which are only mentioned in the Materials and methods) as well as their extensive PCR data compared with the Illumina resequencing data.

3) It might be worth pointing out that type I strains are relatively rare, yet they share the “acute lab mouse” phenotype with many South American strains that not coincidentally share a ROP18 allele (and also ROP5 although this is less well characterized). If it were only clonal type I strains at play, the evolutionary argument that natural hosts must somehow be resistant to such strains would be much less meaningful (type 1 strains are less than 5% of all strains encountered in the wild).

4) The extreme polymorphism of the IRG family of proteins suggests that other pathogens might also impinge on this pathway. This is only briefly alluded to in the Discussion, but in fact it seems relevant from the opening and should be mentioned in the Introduction already. The present work reveals the role of one component of the IRG family in combating Tg. And yet, the role of many other components remains undefined. Again, how important co-evolution with T. gondii is for IRG diversification is still unknown. Along these lines, the observation that the virulent strain GT1 can form cysts in resistant CIM mice is interesting, but not a very strong argument for co-evolution. Virulent strains of T. gondii can form cysts in any resistant species. In this regard, there does not seem anything particularly different about the wild mouse. It is the lab mouse that is the exception. It remains an interesting historical artefact that the extreme susceptibility of the lab mouse led to ROP kinase discovery. Along these lines, little is said about the coincidence between different IRG genotypes and T. gondii strains in the wild, which is obviously important to determine the significance of the laboratory observations.

5) The life cycle cartoon contains several errors. Oocysts contain two sporozoites each with four sporozoites, not two as shown. Tachyzoites are shown outside cells but should be replicating within them. Asexual stages also occur in the cat.

https://doi.org/10.7554/eLife.01298.027

Author response

The reviewers […] felt that some of the evolutionary inferences are overstated. For example, the abstract begins with “Virulence in T. gondii for its natural intermediate hosts, the mouse, is an evolutionary paradox…

Well, paradoxes are there to be resolved. While there is a literature with which we are familiar, and now cited, showing that individuals of other species (including man, of course) are resistant to many virulent T. gondii strains, the belief is general and has been reiterated for years, that mice are peculiarly vulnerable to the parasite. This generalisation has however been based on a limited database: until our own study, which is now pretty well known in the community, everybody has used classical laboratory mouse strains to examine this question. Yes, it's pretty obvious, but as said, (A) it can't be found in the published literature and (B) paradoxes are there to be resolved: this paper presents experimental data that resolve the paradox. Incidentally it is far from obvious that the laboratory strains of mouse should be so homogeneous in the IRG gene clusters. The MHCs of the same strains are strikingly polymorphic. Their shared vulnerability to type I Toxoplasma strains is the case simply because they have homogenised specific haplotypes of the IRG system. One may wonder whether this is due to an undiscovered fitness cost of certain IRG haplotypes rather than to a limited genetic basis among the lab strains.

This would only be the case if one takes laboratory mice, known to be highly derived, as representative of wild mice. In fact, prior studies have shown that deer mice (Peromyscus) are resistant to virulent strains of T. gondii (Am. J. Trop. Med. Hyg. 2, 390-415 (1953), as is the case for other common hosts such as chickens and rats. It is therefore reasonable to assume that wild house mice might express resistance mechanisms not found in laboratory mice, given the extremely narrow genetic diversity of the latter. The authors find such a mechanism and this is certainly interesting, but perhaps not as surprising as claimed.

We do not say anywhere that it is surprising: indeed it was the original apparently general “susceptibility” that was surprising, that was the “paradox”.

Hence, one could conclude that the virulence mechanisms of the parasite have evolved for “resistant hosts” in general and not wild mice per se.

Not sure we understand this statement. Considering what virulence and resistance consist of, i.e. specific interactions between polymorphic molecules of host and pathogen, we do not really grasp what is meant by “‘resistant hosts’ in general”. As we show in Figure 2, there are multiple alleles for relevant resistance molecules: we have explored the resistance properties of a minute sample of the available allelic variation against a minute sample of virulence alleles. It is plausible that further resistance variants may confer protection against further virulence variants.

Although the system seems to resemble the R gene-avirulence gene systems for plants and their pathogens, the co-evolution arguments should be toned down considerably, as the work stands on its own.

We have lowered the prominence of our remarks on plant R-genes. Still, it is a conjecture based on properties the two systems have in common, but not implausible and good to think about, we find. To instance some of the similarities: both are encoded in short, uninterrupted clusters; both are deeply polymorphic; both show evidence of dynamic genomic behaviour within clusters; both show simple resistance/susceptibility behaviour confronted with strain-specific pathogen variants. The MHC shows these properties too, but MHC-related associations with infectious disease resistance and susceptibility are usually much less clear-cut, for a number of reasons.

In summary, we appreciate the various reservations raised above and have made some modifications to the text. Nevertheless the views are ours and, unlike the experimental results themselves (hopefully), can be strengthened or weakened by future experiments. We feel that we should be entitled to express our views clearly, so long as they are not evidently wrong, even if a referee is not of the same opinion.

1) There is a concern about the mechanism proposed for Irgb2-b1 function. In cells from CIM mice infected with RH parasites, the authors demonstrate a substantial increase in Irgb6 and Irgb2-b1 loading onto the parasite vacuole, compared to cells from B6 mice. In addition, although there is undetectable p-Irga6CIM in CIM cells infected with RH parasites, Irga6CIM can be phosphorylated if transfected into other cells (L929 cells), suggesting that Irga6 phosphorylation is actively inhibited in CIM cells. The authors state that Irgb2-b1CIM inhibits the phosphorylation of endogenous Irga6 in CIM mice. This statement is based on experiments in which they transfect Irgb2-b1CIM into B6 cells stimulated with IFN-γ and observe a reduction in p-Irga6 localization to the parasite vacuole membrane, which they quantify. However, they do not directly show that Irgb2-b1CIM inhibits Irga6 phosphorylation. It seems that an alternate interpretation of these data (from Figure 6E,F) is that Irgb2-b1CIM may inhibit the localization of Irga6 to the vacuole, where it is then phosphorylated. This point should be addressed/clarified.

The referee is correct, the data presented is open to another interpretation; thanks for pointing it out. Indeed we already have the data, which we now present as an additional image in Figure 6G, that tests the alternative hypothesis suggested by the referee. In the images originally presented we showed only the deficit of p-Irga6 at vacuoles loaded with Irgb2-b1CIM. However using an antibody against total Irga6 we also measured the loading of total Irga6 at vacuoles loaded with Irgb2-b1CIM. New Figure 6G shows that, so far from being reduced as predicted by the referee's hypothesis, the loading of un-phosphorylated, and thus active, Irga6 is significantly increased by the presence of Irgb2-b1CIM. This result is expected if the absence of p-Irga6 shown in Figure 6F is because the Irgb2-b1CIM acts, as proposed, as a decoy for the parasite kinase complex, drawing it away from its intended target, rather than as an inhibitor of Irga6 binding. Note that binding of Irgb6 at the vacuole is also increased by the presence of Irgb2-b1CIM (Figure 6H).

Overall, the data implicating ROP5 in the loading of Irgb2-b1CIM onto the vacuole surrounding virulent parasites is very indirect. Granted it is decreased in Δrop5 parasites, but this does not equate to a model where Irgb2-b1 binds to ROP5 or inhibits its activity. Other indirect mechanisms are possible. This portion of the manuscript is too speculative. Either concrete data need to be provided or this should be extensively modified to indicate it might result from direct or indirect binding. Does Irgb2-b1 have a direct effect on ROP18? It would look the same phenotypically (Δrop5 parasites would phenocopy this). The model promoted here suggests that ROP5 binds to IRGs. If this interaction is robust, it should be straightforward to demonstrate this for Irgb2-b1.

The referee is correct that the evidence we present is indirect, though it is internally consistent, and externally consistent with the already published evidence for the direct interaction between virulent ROP5 and Irga6, with a precise structural basis, and shortly to be published as a crystal structure of the binary complex (from the Boothroyd lab and shortly to be published as a crystal structure of the binary complex [from Michael Reese and John Boothroyd's lab, PDB codes 4LV5 (ROP5Bi:Irga6) and 4LV8 (ROP5Ci:Irga6) to be released 25/09/2013]). It is important to stress that the IRG proteins are a homologous family and there is every reason to expect that they have homologous behaviour at a structural level. The participation of helix 4 of Irga6 in this interaction is thus also consistent with the evidence we present that helix 4 of Irgb2-b1 is under divergent selection (Figure 7C), if Irgb2-b1 acts as a competitor of Irga6 for ROP5 and interacts directly with the virulence factor. The analysis of the mechanism by which Irgb-2b1 inhibits phosphorylation of Irga6 is not the main theme of this paper. On the basis of our results we consider that a direct interaction is the likeliest hypothesis, but a fuller analysis is beyond the scope of this paper. While these results are suggestive, further experimental analysis is needed to establish whether Irgb2-b1 indeed interacts directly with ROP5.

If such data are elusive, the authors should probably rethink their model.

To be clear, this is not yet a model; it is our present interpretation of the data and we consider it to be the most parsimonious. Certainly other interpretations are possible. The detail of the inhibitory mechanism is not central to the main argument of our paper, though of course is of great interest in itself.

Figure 7 is somewhat speculative, and is also not well explained. For example, what is the significance of the IIa/IIs ratios in the graphic?

The legend of Figure 7C has been modified to explain this data more clearly. Here is a more detailed account:

The πa/πs ratio gives an indication of the frequency with which coding variants occur in a sequence compared with the frequency of non-coding variants. It is related to the dN/dS parameter often used. The analysis is based on a moving window along the sequence and the colour coding records the regions with different frequencies of coding substitutions. The extended “red” domain on and adjacent to helix 4 identifies a region that appears to be under recent positive selection for a divergent sequence. This would occur, e.g., if this region of the molecule was under pressure to interact with new variants of a pathogen protein. Normally one would not expect to see this effect if Irgb2-b1 interacts with a host protein to “bridge” to ROP5 because the interaction surface between two host proteins would not be expected to evolve rapidly under divergent selection. We have added an additional comment on this to the text.

In the last paragraph of the Results when the authors describe Figure 7, they state, “Preliminary results suggest that Irgb2-b1CIM may bind directly to the protein product of the virulent allele of ROP5.” What are these preliminary results? Including this interaction data would significantly strengthen the authors’ model that Irgb2-b1CIM binding to ROP5 may contribute to blocking Irga6 phosphorylation in CIM mice.

The “preliminary results” are those specified in the paper and shown in Figure 7. We are working on this issue at the biochemical level as part of a further study on the details of the mechanism by which Irgb2-b1 interferes with Irga6 phosphorylation. As stated above, we feel we have made a good indirect case for a direct interaction with the ROP5 & ROP18 kinase complex, but a conclusive test is not straightforward. We consider it outside the scope of our paper and hope that the editors are prepared to leave this as it is.

2) The authors rely at least in part on Illumina resequencing data for their phylogenetic studies; such data are notoriously problematic for highly polymorphic and complex, duplicated regions of the genome. It would be appropriate for the authors to discuss how the BAC sequences they generated (which are only mentioned in the Materials and methods) as well as their extensive PCR data compared with the Illumina resequencing data.

To answer the referees in short, the Illumina resequencing is exceptionally reliable. To be clear, however, the only Illumina-derived transcriptome data of our own (at CCG Cologne) that we present here is the CIM transcriptome from IFNγ-induced DDC. The MSM BAC was sequenced by Illumina at Beijing Genomics Institute, de novo assembled then aligned to the C57BL/6 canonical genome. To test the validity of using Illumina resequencing of complete transcriptomes as a general approach for the rapid determination of unknown IRG haplotypes, we made an Illumina run on RNA of IFNγ-induced C57BL/6 fibroblasts to compare with the canonical genomic sequence of this strain that was established by extended Sanger sequencing on shotgun genomic fragments and from BACs. The C57BL/6 haplotype on Chr 11 is peculiarly difficult because it contains 4 very recently divergent tandem Irgb genes and additionally a duplicated Irgb6 gene, the two isoforms differing by only 3 non-coding nucleotides in the ORF. The resequencing results were not only easy to align against the canonical sequence, but also acted as a welcome confirmation of the sequences of the C57BL/6 genome: it was unclear for earlier releases of the C57BL/6 genome whether indeed there was an additional duplication in the IRGB region of Chr 11. The Illumina results show beyond doubt that both the extra copy of Irgb6 and the two extra tandem Irgb genes are real. All the intact IRG genes were recovered in up to 12,000 fold coverage; only degraded pseudogenes were not found. A second comparison was made with the CAST/Ei sequence. In that case, the data from the Sanger mouse genome project was based on Illumina resequencing while our data was based on Sanger sequencing of shotgun genomic fragments from BACs. Again there is perfect conformity between the two sequence sets.

Inevitably, Illumina resequencing of a transcriptome is unable to establish haplotypic linkage structure in heterozygotes carrying two IRG haplotypes, as is common in wild mice. The Sanger genomic Illumina data presented in this paper, however, is all obtained from inbred mice, whether of wild origin or laboratory strains.

Our resequencing reads and alignments are available if the referees would like to confirm their quality for themselves. The strength of the data is not only its consistency, but also the extreme depth of coverage, enabling us to identify and fully sequence IRG genes with expression levels over a 50x range or more. This favourable sensitivity is presumably achieved by the massive induction of IRG genes by IFNγ: we collect RNA from cells stimulated for 24 hr with high concentrations of IFNγ. At this time, IRG gene sequences may reach 1.0% of the total transcriptome.

3) It might be worth pointing out that type I strains are relatively rare, yet they share the “acute lab mouse” phenotype with many South American strains that not coincidentally share a ROP18 allele (and also ROP5 although this is less well characterized). If it were only clonal type I strains at play, the evolutionary argument that natural hosts must somehow be resistant to such strains would be much less meaningful (type 1 strains are less than 5% of all strains encountered in the wild).

The referees are correct that Type I strains are rather uncommon in much of the Eurasian domain; however, they appear to be relatively abundant in some parts of the Far East, e.g., (Puvanesuaran, et al. 2013) Malaysia, 100% type I; (Kyan, et al. 2012) Japan, 6/14 type I; (Zakimi, et al. 2006) Japan 22/49 type I. It is perhaps relevant that the highly resistant and very similar IRG haplotypes of the CIM and CAST/Ei strains are both from the Mm castaneus subspecies local to Southeast Asia. We feel, however, that to stress such correlations on such limited data may encourage various speculations when what is needed is a detailed ecological analysis from the wild environment that we are presently undertaking, coupled with direct experimental support.

4) The extreme polymorphism of the IRG family of proteins suggests that other pathogens might also impinge on this pathway. This is only briefly alluded to in the Discussion, but in fact it seems relevant from the opening and should be mentioned in the Introduction already.

With all respect for the referees’ view, we feel we deal with this important point quite comprehensively, devoting more than a third of the entire Discussion to it. It is a very interesting issue, although it does not bear directly on the results that we present in this paper. It is clear from the data that polymorphism in at least one of the IRG proteins on chromosome 11 is key to control of Toxoplasma virulence. In our view it is premature to speculate intensively on what might be the adaptive meaning of the rest of the polymorphism. Maybe it has to do with other Toxoplasma virulence genotypes; maybe with resistance properties of other pathogen species. Certainly the complete set of mouse pathogens under IRG control is not yet known, but equally certainly it is a surprisingly small subset of the entire range.

Unless the editors insist, we would rather not raise this very interesting but in our view secondary point in the Introduction when we devote a considerable amount of space to it in the Discussion.

The present work reveals the role of one component of the IRG family in combating Tg. And yet, the role of many other components remains undefined. Again, how important co-evolution with T. gondii is for IRG diversification is still unknown. Along these lines, the observation that the virulent strain GT1 can form cysts in resistant CIM mice is interesting, but not a very strong argument for co-evolution.

Agreed. Still, if only certain wild haplotypes are permissive for encystment by “virulent” strains, this does have, let us say, co-evolutionary potential.

Virulent strains of T. gondii can form cysts in any resistant species. In this regard, there does not seem anything particularly different about the wild mouse. It is the lab mouse that is the exception. It remains an interesting historical artefact that the extreme susceptibility of the lab mouse led to ROP kinase discovery.

A wild mouse homozygous for lab mouse chromosome 11 haplotypes would almost certainly be susceptible to virulent strains of T. gondii (though perhaps there are some resistant haplotypes on chromosome 18, even if not in CIM). Note that no other locus in the (CIM x BL/6) x BL/6 cross was able to compensate for the homozygous susceptible Chr 11 locus. Yet these mice are mongrels for the rest of the genome. We haven't found a perfect lab haplotype in a wild mouse yet although some are pretty close, but we haven't looked at very many yet either. Are the referees proposing that the haplotype itself is a laboratory artefact?

Presumably mice with these haplotypes are out there somewhere. We would rather say that the lab mouse a haplotypes, which are highly susceptible to virulent strains, constitute an accidental outcome of the breeding history of the mouse rather than an artefact (unless, as mentioned above, other haplotypes carry a cost under lab conditions).

Along these lines, little is said about the coincidence between different IRG genotypes and T. gondii strains in the wild, which is obviously important to determine the significance of the laboratory observations.

To our knowledge, nothing is known about this. The relevance and importance of the IRG system in controlling T. gondii is a recent discovery; its genetics is the content of this paper and has not been reported before. The referees raise what is obviously an important development from the present study, a development in which the present authors are presently fully engaged.

https://doi.org/10.7554/eLife.01298.028

Article and author information

Author details

  1. Jingtao Lilue

    Institute for Genetics, University of Cologne, Cologne, Germany
    Contribution
    JL, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Urs Benedikt Müller
    Competing interests
    The authors declare that no competing interests exist.
  2. Urs Benedikt Müller

    Institute for Genetics, University of Cologne, Cologne, Germany
    Contribution
    UBM, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Jingtao Lilue
    Competing interests
    The authors declare that no competing interests exist.
  3. Tobias Steinfeldt

    Institute for Genetics, University of Cologne, Cologne, Germany
    Contribution
    TS, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.
  4. Jonathan C Howard

    1. Institute for Genetics, University of Cologne, Cologne, Germany
    2. Fundação Calouste Gulbenkian, Instituto Gulbenkian de Ciência, Oeiras, Portugal
    Contribution
    JCH, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    jhoward@igc.gulbenkian.pt
    Competing interests
    The authors declare that no competing interests exist.

Funding

Deutsche Forschungsgemeinschaft (SFB 680, SFB 635, SFB 670 and SPP 1399)

  • Jingtao Lilue
  • Urs Benedikt Müller
  • Tobias Steinfeldt
  • Jonathan C Howard

International Graduate School in Development Health and Disease

  • Urs Benedikt Müller

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Claudia Poschner for outstanding technical support. Stephanie Könen-Waisman helped with double-blind evaluation of IRG protein loading onto T. gondii vacuoles. We thank Miriam Linnenbrink and Christine Pfeifle (MPI Evolutionary Biology, Plön) for expertise in handling wild mice, and Christine Pfeifle for maintaining the CIM strain in Plön. Annie Orth and François Bonhomme (U Montpellier) provided the CIM breeding colony. Uma Ramakrishnan and colleagues at the NCBI, Bangalore provided the facilities to prepare cells from locally caught wild mice. Olaf Utermoehlen (U Cologne) provided facilities for the infection of wild mice with T. gondii. Carsten Lüder (U Göttingen) and Ildiko Dunay (U Magdeburg) advised on identification and analysis of T. gondii cysts in vivo. Aurélien Tellier (LMU, Munich) and Stephan Schiffels (U Cologne) contributed to analysis of the sequence alignments. We are grateful to Paul Schulze-Lefert (Cologne), Paul Schmid-Hempel (Zurich) and Isabel Gordo (Oeiras) for comments on the manuscript.

Ethics

Animal experimentation: All animal experiments were conducted under the regulations and protocols for animal experimentation by the local government authorities (Bezirksregierung Köln, Germany), LANOV Nordrhein-Westfalen Permit No. 44.07.189.

Reviewing Editor

  1. Detlef Weigel, Reviewing Editor, Max Planck Institute for Developmental Biology, Germany

Publication history

  1. Received: July 31, 2013
  2. Accepted: September 13, 2013
  3. Version of Record published: October 29, 2013 (version 1)

Copyright

© 2013, Lilue et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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