Death domains (DDs) mediate assembly of oligomeric complexes for activation of downstream signaling pathways through incompletely understood mechanisms. Here we report structures of complexes formed by the DD of p75 neurotrophin receptor (p75NTR) with RhoGDI, for activation of the RhoA pathway, with caspase recruitment domain (CARD) of RIP2 kinase, for activation of the NF-kB pathway, and with itself, revealing how DD dimerization controls access of intracellular effectors to the receptor. RIP2 CARD and RhoGDI bind to p75NTR DD at partially overlapping epitopes with over 100-fold difference in affinity, revealing the mechanism by which RIP2 recruitment displaces RhoGDI upon ligand binding. The p75NTR DD forms non-covalent, low-affinity symmetric dimers in solution. The dimer interface overlaps with RIP2 CARD but not RhoGDI binding sites, supporting a model of receptor activation triggered by separation of DDs. These structures reveal how competitive protein-protein interactions orchestrate the hierarchical activation of downstream pathways in non-catalytic receptors.https://doi.org/10.7554/eLife.11692.001
Cells have proteins called receptors on their surface that can bind to specific molecules on the outside of the cell. Typically, this binding activates the receptor and the activated receptor then triggers some biochemical changes inside the cell. For many receptors, the portion of the receptor inside the cell is essentially an enzyme that can trigger a biochemical change by itself. Some receptors, however, lack any enzymatic activity, and it is often unclear how these ‘non-catalytic receptors’ trigger changes inside a cell.
A protein called p75 neurotrophin receptor (or p75NTR for short) is a non-catalytic receptor that is expressed when neurons are injured and its activity leads to the death of the neurons and related cells. Inhibiting this non-catalytic receptor is an attractive strategy for limiting the damage caused by diseases of the nervous system. However, the molecular mechanisms behind the activity of p75NTR are not well understood.
Previous biochemical studies set out to answer the question of how p75NTR engages with components of the signaling machinery inside the cell, and found several components that interact with this receptor. Now, Lin et al. have tried to gain a more detailed understanding of those interactions at a molecular level. This involved solving the three-dimensional structures of three protein complexes that involve part of p75NTR (called the “death domain”) and one of two signaling components (called RhoGDI and RIP2).
Two of the protein complexes showed that RIP2 and RhoGDI bind to the receptor’s death domain at partially overlapping sites, although RIP2 binds about 100 times more strongly than RhoGDI.A third protein complex showed an interaction between two copies of the death domain, which involves a surface of the receptor that overlaps with RIP2’s, but not RhoGDI’s, binding site.
These structures, together with the results of other experiments, allowed Lin et al. to propose a model that could explain how p75NTR is activated. First, the two death domains must be separated. Next, RIP2 is recruited to the receptor, and outcompetes and displaces RhoGDI. This change in protein-protein interactions switches the receptor’s signaling from one pathway to the other. Now that these structures are available, they can be used in future experiments to design specific changes in the receptor that would allow researchers to dissect its different activities.https://doi.org/10.7554/eLife.11692.002
The death domain (DD) is a globular protein module of 80–90 amino acid residues with a characteristic six-helix bundle fold (Feinstein et al., 1995; Ferrao and Wu, 2012). DDs are present in a variety of proteins, including several members of the tumor necrosis factor receptor superfamily (TNFRSF) and a range of intracellular signaling components, such as caspases and kinases. The DD family includes four subfamilies of structurally related domains, including the canonical DD, the death effector domain (DED), the caspase recruitment domain (CARD), and the pyrin domain (PYD). DD-containing proteins play central roles in apoptotic and inflammatory signaling through the formation of oligomeric protein complexes, and several disease-causing mutations have been mapped to DD interfaces (Park et al., 2007). All DD complexes described so far involve homotypic interactions between DDs of the same subfamily (e.g., DD with DD, CARD with CARD, etc.). All known DD interactions belong to one of three types (I to III), each mediated by conserved asymmetric interfaces in the interacting DDs (Park, 2011; Park et al., 2007; Weber and Vincenz, 2001). Heterotypic complexes between DDs from different subfamilies have not yet been described and, aside from a few structures of DDs bound to small polypeptides, no complexes of DDs with proteins outside the DD superfamily have been reported. Thus, type I, II, and III interactions between DDs are thought to represent the predominant mechanism of oligomerization and complex formation for DD-containing proteins.
The cytoplasmic domain of the p75 neurotrophin receptor (p75NTR, also known as NGFR and TNFRSF16) contains a C-terminal DD connected to the transmembrane (TM) domain by a 60-residue-long linker region (Liepinsh, 1997). p75NTR is a receptor for members of the neurotrophin family, such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (Dechant and Barde, 2002; Roux and Barker, 2002). In addition to the neurotrophins, a number of other extracellular ligands can also engage or signal through p75NTR, including the beta-amyloid peptide (Knowles et al., 2009; Perini et al., 2002), the rabies virus glycoprotein (Tuffereau et al., 1998), and various myelin-derived polypeptides (Wang et al., 2002; Wong et al., 2002). p75NTR may function alone or in conjunction with other transmembrane proteins to allow ligand binding and intracellular signaling. These proteins include members of the Trk family of receptor tyrosine kinases, members of the Vps10p family of sorting receptors, such as Sortilin, and the Nogo receptor, which promotes binding to myelin-derived ligands (Underwood and Coulson, 2008). p75NTR can engage different intracellular pathways, of which the best characterized are the RhoA pathway, which regulates axon growth, collapse and degeneration (Park et al., 2010; Yamashita et al., 1999; Yamashita and Tohyama, 2003), the NF-kB pathway, which contributes to cell survival (Carter et al., 1996; Khursigara et al., 2001; Vicario et al., 2015), and the c-Jun kinase (JNK) or caspase pathway, which mediates apoptotic cell death (Friedman, 2000; Yoon et al., 1998). p75NTR signaling through any of those three pathways requires a functional DD (Charalampopoulos et al., 2012). Expression of p75NTR increases in a number of neurodegenerative diseases and upon injury or stress conditions, where it contributes to neuronal and glial cell damage, axonal degeneration, and synaptic dysfunction (Ibanez and Simi, 2012). Inhibition of p75NTR signaling has emerged as an attractive strategy for limiting neural damage in neurodegeneration and nerve injury.
The mechanism of activation of p75NTR in response to neurotrophins involves a conformational rearrangement of disulfide-linked receptor dimers, resulting in the separation of intracellular DDs (Vilar et al., 2009). Fluorescence resonance energy transfer (FRET) experiments have shown that the two DDs in the p75NTR dimer are in close proximity to each other (high FRET state) and that NGF binding induces a decrease in FRET signal (Vilar et al., 2009). Disruption of this conformational change through mutation of a conserved cysteine residue in the TM domain prevents p75NTR signaling in response to neurotrophins (Vilar et al., 2009). p75NTR lacks an associated catalytic activity. Similar to other members of the TNFRSF, signaling by p75NTR proceeds via ligand-induced recruitment and release of cytoplasmic effectors to and from its intracellular domain. Ligand-induced separation of p75NTR DDs may allow the recruitment of intracellular components for downstream signal propagation. Although a variety of intracellular proteins have been reported to interact with p75NTR, the molecular mechanisms by which the receptor engages different signaling pathways remain unclear. To begin addressing these questions, our laboratory performed a comprehensive structure–function study of the p75NTR DD that resulted in the identification of three sets of solvent-exposed residues that are critical for p75NTR’s ability to engage the RhoA, NF-kB and JNK/cell death pathways, respectively (Charalampopoulos et al., 2012). Receptor mutants that are selectively deficient in one pathway but not others were generated, demonstrating that different signaling outputs can be genetically separated in p75NTR. Understanding how such interfaces relate to each other and to the mechanism of receptor activation has remained an important challenge.
In this study, we have undertaken a structural biology approach to elucidate the molecular mechanisms underlying downstream signaling mediated by the DD in p75NTR. We have determined the solution structures of the p75NTR DD in complex with RhoGDI (Rho guanine nucleotide dissociation inhibitor), which links the receptor to the RhoA pathway (Yamashita et al., 1999; Yamashita and Tohyama, 2003), or with the CARD domain of RIP2 kinase, which is necessary for p75NTR coupling to the NF-kB pathway (Charalampopoulos et al., 2012; Khursigara et al., 2001). We have also solved the solution structure of the p75NTR DD homodimer, revealing the DD surface that is occluded prior to neurotrophin-mediated receptor activation. These structures uncover novel heterotypic DD interactions, not previously seen in other DD-containing complexes, and reveal the molecular mechanisms underlying the early stages of p75NTR activation and downstream signaling.
RhoGDI interacts constitutively with the DD of unliganded p75NTR, linking the receptor to the RhoA pathway (Yamashita and Tohyama, 2003). Neurotrophin binding induces the release of RhoGDI from p75NTR and decreases RhoA activity (Gehler et al., 2004; Yamashita et al., 1999; Yamashita and Tohyama, 2003). Using biochemical and cell-based assays, we have previously identified solvent-exposed residues in the p75NTR DD that are critical for its interaction with RhoGDI and RhoA activation, including residues in helices H1 and H6 (Charalampopoulos et al., 2012). In order to obtain a molecular understanding of this interaction, we determined the solution structure of the human p75NTR DD:RhoGDI complex by multidimensional nuclear magnetic resonance (NMR) spectroscopy (Figure 1—figure supplement 1 and Table 1). We note that, unless otherwise indicated, all amino acid residue numbering in this study corresponds to the human forms of the respective proteins. Human and rat p75NTR DD share more than 90% sequence identity—including all functionally relevant residues—and an essentially identical three-dimensional structure with an overall RMSD of 1.7 Å ((Liepinsh, 1997) and this study). The ensemble of the 10 lowest-energy conformers of the DD:RhoGDI complex and a representative structure are depicted in Figure 1A,B. p75NTR DD in the complex consists of one 310-helix followed by six α-helices and its global fold is very similar to that of our previously described structure of rat p75NTR DD (Liepinsh, 1997). In the complex, the C-terminal domain of RhoGDI primarily displays an immunoglobulin-like fold similar to previously described structures of this protein (Longenecker et al., 1999). Residues Glu40 to Gly57 in the RhoGDI N-terminal domain fold into a long helix, which is not involved in p75NTR DD binding and remains flexible in the complex (Figure 1A). Removal of this N-terminal domain did not affect RhoGDI binding to p75NTR DD (Figure 1—figure supplement 2). Inspection of the interface in the complex showed that it is mainly formed by α-helices H1 and H6 of the p75NTR DD and β-strands S2, S3, S9 and α-helix H2 of RhoGDI, forming a small hydrophobic core surrounded by polar residues (Figure 1C). Charged residues play an important role in the binding interface and high concentration of salt (i.e., greater than 200 mM NaCl) can almost completely disrupt p75NTR DD:RhoGDI interaction in vitro (Figure 1—figure supplement 1C). It is gratifying to note that all functional DD determinants that we have previously identified by site-directed mutagenesis clustered at the DD:RhoGDI interface of the complex structure (labeled red in Figure 1C,D). The structure of the DD:RhoGDI complex offered an opportunity to address the functional importance of a larger set of residues in the p75NTR DD as well as in RhoGDI. Co-immunoprecipitation experiments were performed in cells transfected with constructs of full-length p75NTR and RhoGDI carrying different point mutations in selected residues. Alanine substitution of individual amino acid residues likely uncovers only those side chains making the most critical contribution to the binding interface. In the p75NTR DD, substitution of either Asp412, Lys343 or Glu420 was found to significantly diminish interaction with RhoGDI (Figure 1D). In RhoGDI, substitution of Lys99 or Lys199 abolished its interaction with p75NTR (Figure 1E). In agreement with this, the structure of the complex shows that these two positively charged side chains make charge interactions with Glu420 and Asp412, respectively, in the p75NTR DD (Figure 1C).
Analysis of the p75NTR DD:RhoGDI complex and a previously described crystal structure of the RhoGDI:RhoA(GDP) complex (Longenecker et al., 1999) indicated that p75NTR DD and RhoA interact with different surfaces in RhoGDI, located at opposite sides of the molecule. The two distant binding sites on RhoGDI suggested that a heterotrimer complex p75NTR DD:RhoGDI:RhoA may be structurally feasible. We investigated this by performing HADDOCK calculations (Dominguez et al., 2003) using our solution structure of p75NTR DD from its complex with RhoGDI and the crystal structure of the RhoGDI:RhoA(GDP) complex. Multiple refinements converged to a mean backbone root mean square deviation (RMSD) of 0.64 ± 0.05 Å (Figure 2A,B and Table 2). Ramachandran plot analysis of the docking model indicated that the trimer structure, including the two intermolecular interfaces, still occupies the energetically preferred conformation. In the tripartite complex, the N-terminal domain of RhoGDI folded into two helices and bound to RhoA(GDP), similar to its conformation in the RhoGDI:RhoA(GDP) complex (Longenecker et al., 1999). The DD binding site on RhoGDI remained nearly identical to that in the DD:RhoGDI complex. This analysis shows that interaction of the three proteins can indeed occur simultaneously and explains previous biochemical studies showing that RhoA can be co-immunoprecipitated with p75NTR in the presence of RhoGDI (Yamashita et al., 1999). Using the purified proteins, we determined the binding affinity of the RhoGDI:RhoA complex by surface plasmon resonance (SPR). Titration of RhoGDI onto immobilized RhoA, yielded a binding Kd of 0.14 ± 0.01 μM, which is in agreement with previous measurements (Tnimov et al., 2012) (Figure 2C). Interestingly, when RhoGDI was precomplexed with purified p75NTR DD, the Kd was 2.2 ± 0.11 μM (Figure 2D), indicating that binding to the p75NTR DD decreases the affinity of the RhoGDI:RhoA interaction by about 15-fold. We note that, in the absence of RhoGDI, no binding between p75NTR DD and RhoA could be detected in these experiments (Figure 2E). These results suggest that RhoGDI binding to the p75NTR DD weakens its interaction with RhoA, a step which may facilitate RhoA activation.
NGF binding to p75NTR elicits the recruitment of RIP2 kinase to the receptor. Recruitment of RIP2 is required for regulation of the NF-kB pathway by p75NTR. Previous biochemical studies established that the interaction between p75NTR and RIP2 is mediated by their DD and CARD domains, respectively (Khursigara et al., 2001). The RIP2 CARD consists of 107 amino acids and is located in the C-terminal of the protein. It connects to the N-terminal kinase domain via a linker of 120 amino acids. We determined the NMR structure of human RIP2 CARD in monomeric form (Figure 3A,B and Table 1). The solution structure of RIP2 CARD comprises an arrangement of six α-helices followed by one short 310-helix, all tightly packed around a hydrophobic core. A C-terminal tail of 17 amino acids (Leu524-Met540) follows the CARD and is unstructured and flexible in solution. A unique segment (Gln518-Ile523) between the C-terminal tail and the 310-helix contains two structural disruptor residues (i.e., Pro519 and Pro520, Figure 3B) and lacks a secondary structure, but its orientation was well-defined in the NMR structure. A number of hydrophobic residues (e.g., Ile523, Figure 3B) in this segment closely interact with the first and the last α-helices in the RIP2 CARD. Structural comparison using the DALI server (Holm and Rosenström, 2010) showed that the most similar structure to RIP2 CARD was the CARD of nucleotide-binding oligomerization domain-containing protein 1 (NOD1), with a Z-score between 9 and 11. NOD1 and RIP2 have been shown to interact with each other through their CARDs to propagate immune signaling (Mayle et al., 2014). The two CARDs share similar structural features, including a similar arrangement of all but the last of the α-helices, which displays different local secondary structures in the two proteins (Figure 3—figure supplement 1A). Despite their folding similarities, the two CARDs exhibit significantly different surface characteristics. Particularly, RIP2 CARD has many more charged residues on its surface than its NOD1 counterpart (Figure 3—figure supplement 1B,C). Different electrostatic surfaces will confer different interaction specificities, a common feature among members of the DD superfamily, including the CARD subfamily.
In order to obtain a molecular understanding of the p75NTR DD:RIP2 CARD interaction, we determined the NMR structure of the complex. Figure 3C,D present the three-dimensional solution structure of this complex obtained from the NMR experimental restraints (Table 1 and Figure 3—figure supplement 2). The core helical structure of the p75NTR DD in the p75NTR DD:RIP2 CARD complex was similar to that in the p75NTR DD:RhoGDI complex, with a pairwise RMSD of ~1.9 Å (Figure 3—figure supplement 3A). The orientation of α-helices H3 and H6 changed by approximately 7°–8°. Similarly, pairwise RMSD between RIP2 CARD in monomeric form and in complex with the p75NTR DD was ~1.5 Å (Figure 3—figure supplement 3B). The main interface in the core structure of the p75NTR DD:RIP2 CARD complex involved α-helices H2, H3, H5, H6, and H5–H6 loop of p75NTR DD and α-helices H1, H3–H4, and H5–H6 loops of RIP2 CARD (Figure 3E,F). Both electrostatic and hydrophobic interactions contribute to the p75NTR DD:RIP2 CARD interface. Interestingly, the C-terminal tail of RIP2 CARD was better defined in its complex with the p75NTR DD compared to its monomeric form, although the last six amino acids still remained flexible. The C-terminal tail bound to α-helices H1, H5, and H6 of p75NTR DD, through both hydrophobic and charged interactions (Figure 3D and Figure 3—figure supplement 3B). In our previous site-directed mutagenesis studies of the p75NTR DD, we had identified residues in helices H2 (Asp357, His361, and Glu365), H3 (Gln369 and Glu371), H5 (Asp399), and H6 (Asp412 and Glu415) as being critical for its interaction with RIP2 (Charalampopoulos et al., 2012). We were pleased to note that all these residues mapped to the DD:CARD binding interface defined in our NMR structure of the complex (labeled red in Figure 3E,F). The NMR structure of the DD:CARD complex offered an opportunity to address the functional importance of residues in the RIP2 CARD. Co-immunoprecipitation experiments were performed in cells transfected with constructs of full-length p75NTR and RIP2, the latter carrying different point mutations in selected residues of the CARD. We found that individual substitution of interface residues Gln437, Asp467, Lys471, Ile496, Glu500, or Arg528, significantly diminished RIP2 interaction with p75NTR (Figure 3G).
Comparison of the DD interfaces used for binding to RhoGDI and RIP2 CARD showed that these shared partially overlapping binding sites on p75NTR DD (Figure 4A), indicating that RIP2 and RhoGDI cannot bind to the p75NTR DD simultaneously due to steric hindrance. This is in agreement with our previous biochemical studies that identified overlapping epitopes required for the interaction of p75NTR DD with both RIP2 and RhoGDI (Charalampopoulos et al., 2012). In order to better understand the hierarchical relationship of these interactions, we determined the binding affinities of the DD:RhoGDI and DD:CARD complexes by SPR. The Kd of RhoGDI binding to the p75NTR DD was 0.82 ± 0.3 μM, while the Kd of CARD binding to the DD was 4.67 ± 0.7 nM (Figure 4B,C), indicating that RIP2 CARD binds with approximately 177-fold higher affinity than RhoGDI to the p75NTR DD. This is in line with the larger buried solvent accessible area in the p75NTR DD:RIP2 CARD complex (~1400 Å2) compared to that in the p75NTR DD:RhoGDI complex (~900 Å2). Kinetic analyses revealed that CARD associates with the p75NTR DD with faster on-rate, and dissociates with slower off-rate, than RhoGDI (Table 3). The RIP2 CARD could still fold into a typical α-helical structure after deletion of the C-terminal tail (Figure 3—figure supplement 4A). However, the binding affinity of this construct to the p75NTR DD was 58.7 ± 8.8 nM, that is, approximately 12-fold lower than with the C-terminal tail (Figure 3—figure supplement 4B), indicating a significant contribution of the C-terminal tail to the association of RIP2 with p75NTR.
The differential binding of RhoGDI and RIP2 CARD to the p75NTR DD was further tested through analysis of 2D NMR spectra of RhoGDI binding to p75NTR DD in competition with RIP2 CARD. Figure 4D shows details of the RhoGDI spectra focusing on representative residues located in and/or close to the DD:RhoGDI interface. Addition of p75NTR DD produced a shift in the cross-peaks of these residues (red in Figure 4D), indicating binding of RhoGDI to the DD. Addition of RIP2 CARD to the RhoGDI:DD complex shifted these cross-peaks back to their initial positions (arrows in Figure 4D), indicating dissociation of RhoGDI from the p75NTR DD. These data demonstrate that RhoGDI and RIP2 CARD compete for binding to the p75NTR DD and that RIP2 CARD can displace RhoGDI from the receptor. In order to test the functional significance of the antagonism between RIP2 and RhoGDI, we assessed the levels of RhoA-GTP, a measure of RhoA activation, in cells transfected with a p75NTR expression construct in the absence or presence of increasing concentrations of a RIP2 construct (Figure 4E). While expression of p75NTR increased RhoA-GTP levels in transfected cells, coexpression of RIP2 decreased RhoA-GTP levels in a concentration-dependent manner (Figure 4E), in agreement with an inhibitory role of RIP2 in p75NTR-mediated activation of the RhoA pathway. In line with this, we found elevated levels of RhoA-GTP in brain extracts from Rip2 knockout mice compared to wild type littermates (Figure 4F), suggesting that RIP2 can also restrict the activation of the RhoA pathway in vivo.
The current model of p75NTR activation by neurotrophins predicts that the DDs should be in close proximity to each other to account for the high FRET state of the unliganded receptor. Purified rat p75NTR DD has been shown to exist in either monomeric form or equilibrium between monomeric and dimeric forms depending on pH and counterion (Vilar et al., 2014). However, the complete assignment of the DD homodimer was not reported in that study. We also found that human p75NTR DD exists mainly in monomeric form in TRIS or HEPES buffer at pH 6.0–7.0, which were the buffer conditions used for structure determination of DD:RhoGDI and DD:CARD complexes. In phosphate buffer, however, we observed a new form of p75NTR DD as revealed by the appearance of a new set of cross peaks in the [1H-15N] HSQC spectrum (Figure 5—figure supplement 1A,B). The set of cross peaks corresponding to monomeric DD was still visible, with nearly identical chemical shift but weaker intensity (Figure 5—figure supplement 1A), suggesting the coexistence of dimeric and monomeric DDs under these conditions. Dynamic lighter scattering (DLS) also indicated the formation of dimeric p75NTR DDs in the presence of phosphate ions (Figure 5—figure supplement 1C,D). EGFP-tagged p75NTR DDs showed anisotropic changes due to homodimerization at different DD concentrations. The apparent Kd of dimerization derived from anisotropic change was 49 ± 15 µM (Figure 5—figure supplement 1E). This relatively low-affinity interaction may facilitate DD separation (low FRET state) upon receptor activation by neurotrophins.
In order to identify the dimerization interface, we determined the NMR structure of the p75NTR DD homodimer. The p75NTR DD homodimer adopted a C2 symmetry (Figure 5A,B). A short C-terminal tail of 7 amino acids (Ser421-Val427) in each monomer remained disordered, similar to the DD:RhoGDI and DD:CARD complexes. The helical bundle, including the 310 helix, did not undergo significant structural change with an RMSD lower than 1.5 Å compared to the other complexes. The dimerization interface consisted of α-helices H2 and H3 as well as residues in the H1–H2 and H3–H4 loops. Dimerization involved both charge and hydrophobic interactions. The key residues in the dimer interface included Asp357, Arg360, Thr377, His378, Glu379, and Ala380 (corresponding to Asp354, Arg358, Thr375, His376, Glu377, and Ala378 in rat p75NTR DD). This is in agreement with previous site-directed mutagenesis and NMR titration studies of the rat p75NTR DD homodimer (Vilar et al., 2014) (red-labeled residues in Figure 5C). Cys381 (homologous to Cys379 in rat p75NTR DD) was also located in the dimerization interface but appeared in reduced form with a Cβ chemical shift of 26.5 ppm. The distance between Cys381Sγ from each monomer was 6.7 ± 0.1 Å, that is, too long for the formation of a disulfide bond. The buried solvent accessible area in the p75NTR DD homodimer was 573 Å2, in line with the low affinity of the DD:DD interaction. We conclude that the two p75NTR DD protomers form a low-affinity, noncovalent homodimer in our structure.
The p75NTR DD homodimer interface defined in our NMR studies did not overlap with the RhoGDI binding site (Figure 6A,B). This is in agreement with the constitutive interaction of RhoGDI with the closed conformation of the receptor. On the other hand, the DD homodimer partially occluded the RIP2 CARD interaction surface (Figure 6C), indicating that interaction of RIP2 with p75NTR requires dissociation of the DD homodimer. We investigated whether the recruitment of RIP2 contributes to the separation of DDs triggered after neurotrophin binding to the receptor. This was done by taking real-time homo-FRET anisotropy measurements of DD:DD interaction in response to NGF in cells transfected with EGFP-tagged constructs of full length wild type p75NTR and a DD mutant deficient in RIP2 binding (Charalampopoulos et al., 2012) as previously described (Vilar et al., 2009). Application of NGF to cells expressing wild type p75NTR produced large oscillations of increased anisotropy at the cell membrane (Figure 6D), resulting in a positive net change averaged over a 15-min treatment compared to vehicle (Figure 6E). As anisotropy is inversely related to FRET, this behavior indicates ligand-triggered separation of receptor intracellular domains, as proposed earlier (Vilar et al., 2009). We note that the oscillations observed here are unlikely to represent the synchronous behavior of ensembles of receptors, as their average period (2–3 min) would seem too slow to reflect real molecular dynamics. Importantly, the p75NTR construct carrying mutations in the CARD binding site (CBS mutant) produced very similar anisotropy changes after NGF treatment (Figure 6D,E), indicating that recruitment of RIP2 is not required for DD separation in response to ligand binding to p75NTR. Finally, we note that the DD homodimer interface also overlapped with several residues involved in neurotrophin-mediated activation of JNK, caspase-3 and the apoptosis pathway (Figure 6F) as identified in our previous studies (Charalampopoulos et al., 2012). Thus, while the structure and biochemical properties of the p75NTR DD homodimer support the ligand-independent interaction of RhoGDI with the receptor, they also demonstrate that dissociation of the p75NTR DD homodimer is required for recruitment of RIP2 and for activation of the JNK/caspase-3 pathway in response to neurotrophins.
The main paradigm in signal transduction by DD-containing proteins is oligomerization via homotypic DD interactions. Although p75NTR contains a DD, which is required for downstream signaling, no intracellular p75NTR effectors containing canonical DDs have been identified. The study of DD signaling in p75NTR therefore addresses an unexplored dimension of the repertoire of interactions and activities in the DD superfamily. Homotypic interactions between DDs have been classified as type I, II, and III according to the interfaces involved (Park, 2011; Park et al., 2007b; Weber and Vincenz, 2001). Despite what might have been expected of homotypic interactions, all known interactions in the DD superfamily are asymmetric, that is, the interaction is mediated by different interfaces in each of the two interacting domains (e.g., Ia and Ib for type I). Remarkably, none of the binding surfaces in the p75NTR DD (or in RIP2 CARD) identified in this study show a close match to any of the six conserved surfaces that characterize classical type I, II, and III interactions in the DD superfamily. The p75NTR DD surface that binds RhoGDI is formed by residues in helices H1 and H6. A previous study had proposed helix 5 as a binding site to RhoGDI based on serial deletion analysis of the p75NTR DD (Yamashita and Tohyama, 2003). This conclusion is not supported by our solution structure of the DD:RhoGDI complex, in which H5 appears at the opposite side of the interface (Figure 2B), nor by previous structure–function studies (Charalampopoulos et al., 2012). This discrepancy highlights some of the pitfalls in serial deletion studies that disregard the three-dimensional structures of proteins. The surface in the p75NTR DD that interacts with the CARD of RIP2 includes residues in helices H2, H3, and H6 plus residues in the H5–H6 loop. On the other side of this interaction, residues in helix H1 as well as the H3–H4 and H5–H6 loops form the binding surface on the CARD of RIP2. To the best of our knowledge, this p75NTR DD:RIP2 CARD complex represents the first structural characterization of an heterotypic interaction in the DD superfamily. Our solution structure of this complex also revealed an additional interaction between residues in the C-terminal half of helix H5 of the p75NTR DD and the C-terminal tail of RIP2, which extends beyond the RIP2 CARD. This additional contact confers approximately fivefold increased binding affinity between the two proteins. Finally, the interface that mediates the p75NTR DD homodimer involves residues located in helix H3 as well as the H1–H2 and H3–H4 loops. This surface is similar, but not identical, to the type IIIb surface, like the one identified in the DD of PIDD for interaction with RAIDD (Park et al., 2007b). Unlike the classical type IIIb surface, however, the DD:DD interaction in p75NTR makes extensive use of residues in the H3 helix, and the same surface in the two interacting DDs is used to form a symmetric dimer. Further studies will be required to determine whether the interactions identified here for the p75NTR DD are exceptions or else represent new types of interactions that are yet to be identified in other DD-containing proteins.
The solution structure of the p75NTR DD homodimer shows it is a symmetric, noncovalent dimer held together by low-affinity interactions involving residues in helix H3 and the H1–H2 and H3–H4 loops. The p75NTR DD dimer interface is in agreement with sites of interaction with downstream effectors identified by the structures reported here and in our previous site-directed mutagenesis studies (Figure 6). This p75NTR DD dimer structure is also in accordance with a recent mutagenesis study that identified residues important for dimerization of rat p75NTR DD (Vilar et al., 2014), many of which are also implicated in our structure. On the other hand, our results do not support two crystallographic structures reported for the rat p75NTR DD homodimer that suggested this to be either a covalent symmetric dimer, held by a disulfide bond between Cys379 residues, or a noncovalent asymmetric dimer (Qu et al., 2013). None of the currently available evidence derived from structural, mutagenesis, or functional studies appears to support those crystal structures. Nevertheless, we cannot at present rule out the possibility that p75NTR DDs may under certain circumstances form alternative oligomeric complexes through different interfaces. A recent study has suggested that p75NTR can form trimers in transfected cells based on the apparent molecular weights of p75NTR species in SDS/PAGE (Anastasia et al., 2015). Our NMR studies of the p75NTR DD do not support such conclusion as we have not found any evidence for the existence of DD trimers in any of the conditions tested. Another recent study has used solution NMR spectroscopy to investigate the mobility of the transmembrane and intracellular domains of p75NTR incorporated into lipid–protein nanodisks (Mineev et al., 2015). These authors found a high level of flexibility in the juxtamembrane domain of p75NTR, an observation that we also reported in our earlier NMR studies of this domain (Liepinsh, 1997), but they could not detect self-association of intracellular domains. However, it is unclear whether the lipid detergent used to form the lipid–protein nanodisks interacted with the p75NTR DD and prevented its dimerization. A few detergent molecules, too few to affect DD rotational correlation time, would be sufficient to hinder DD:DD interactions.
The mechanism underlying ligand-induced dissociation of RhoGDI from p75NTR has remained unclear. As neurotrophins induce the release of RhoGDI and the recruitment of RIP2, we have speculated that RIP2 may displace RhoGDI from binding sites in the p75NTR DD (Charalampopoulos et al., 2012). Our solution structures of the p75NTR DD in complex with RhoGDI and the RIP2 CARD lend experimental support to this notion by showing how steric clashes between the two effector proteins preclude their simultaneous binding to the p75NTR DD. SPR experiments indicated that RIP2 CARD binds with over 100-fold higher affinity to the p75NTR DD than RhoGDI, and 2D-NMR competition studies demonstrated that RIP2 CARD can in fact displace RhoGDI from the receptor. The functional significance of this relationship was evidenced by the ability of RIP2 to decrease p75NTR-mediated RhoA activation in a dose-dependent manner. Furthermore, the enhanced activation of the RhoA pathway observed in brain extracts of Rip2 knockout mice suggests that RIP2 may also restrict activation of this pathway in vivo. These results demonstrate how a hierarchy of binding affinities dictates the differential interaction of downstream effectors with p75NTR and ultimately controls the logic of p75NTR signaling.
p75NTR has been postulated to function as a “displacement factor” that releases RhoA from RhoGDI leading to RhoA activation (Yamashita and Tohyama, 2003). This model has led to the idea that the p75NTR DD and RhoA may compete for binding to RhoGDI. On the other hand, biochemical experiments have shown that RhoA can associate with p75NTR through RhoGDI and the three proteins can be recovered together in co-immunoprecipitation assays (Yamashita et al., 1999; Yamashita and Tohyama, 2003), a result that would be incompatible with the displacement concept. Our structural studies show that the p75NTR DD and RhoA bind on opposites sides of the RhoGDI molecule, allowing the formation of a tripartite DD:RhoGDI:RhoA complex. Using a model of this complex and our solution structure of the p75NTR DD homodimer, we have constructed a model of the hexameric complex of these proteins (Video 1). This model retains the two fold symmetry of the DD homodimer, and accommodates all six components without any steric clashes. How can these interactions lead to RhoA activation? Our SPR experiments showed that association of RhoGDI with the p75NTR DD reduced its affinity for RhoA by 15-fold. Close comparison of RhoGDI structures in the complexes with either p75NTR DD or RhoA revealed local structural perturbations in RhoGDI (Figure 2—figure supplement 1), suggesting a potential allosteric mechanism underlying the release and activation of RhoA upon RhoGDI biding to the receptor. Based on the present studies, we propose a model for the early stages of p75NTR engagement with the RhoGDI/RhoA and RIP2/NF-kB pathways based on differential binding affinities and competitive protein–protein interactions (Figure 7). At the plasma membrane, the p75NTR forms a dimer held together by interactions between its DD and TM domains (Figure 7A). Recruitment of the RhoGDI:RhoA complex brings RhoA close to the plasma membrane (Figure 7B). RhoGDI binding to the p75NTR DD weakens its interaction with RhoA, a lipid-modified protein, allowing it to equilibrate with the plasma membrane where it can be activated by membrane-associated guanine nucleotide exchange factors (GEFs) (Garcia-Mata et al., 2011). RhoA may thus be in equilibrium between the cell membrane and the RhoGDI:p75NTR complex, and the action of GEFs and GTPase-activating proteins (GAPs) may further contribute to the dynamics of this exchange (Figure 7B). Upon dissociation from p75NTR, for example, as a consequence of RIP2 recruitment in response to NGF binding, RhoGDI regains high affinity for RhoA, extracting it from the membrane and holding it back in the cytosol in an inactive state (Figure 7C). This new view of the p75NTR DD in the activation of RhoA is in better agreement with the emerging role of RhoGDI as a general facilitator of small GTPase activity cycles. Recruitment of RIP2 to p75NTR ultimately leads to increased NF-kB activity by as yet unknown mechanisms. Another p75NTR interactor, TRAF6, is also a known regulator of the NF-kB pathway (Khursigara et al., 1999; Ye et al., 1999). As TRAF6 has been shown to interact with the juxtamembrane region of p75NTR, but not with the DD, RIP2 and TRAF6 may be able to bind simultaneously to the receptor and together contribute to enhance NF-kB activity in response to neurotrophins.
The structural studies of DD signaling in p75NTR presented here uncovered novel heterotypic interactions not previously seen in other DD-containing complexes. They represent new ways by which DDs regulate intracellular signaling. NMR, biochemical, and functional studies defined competitive interactions between RhoGDI and RIP2 CARD and between RIP2 CARD and the p75NTR DD homodimer. These interactions give us unique insights into the molecular mechanisms underlying p75NTR activation and signaling, and reveal how overlapping interfaces and differential binding affinities cooperate to orchestrate the hierarchical activation of downstream pathways in noncatalytic receptors.
The cDNAs of human p75NTR DD (330–427), RhoGDI (2–204), RhoA (2–190), and RIP2 CARD (434–539) were amplified from total human embryonic stem (ES) cell cDNA and subcloned into pET32-derived expression vectors between BamH I and Xho I restriction sites. Each recombinant protein contains 16 additional residues (MHHHHHHSSGLVPRGS) at the N-terminal, including one 6×His tag. Unlabeled proteins were expressed in E. coli strain SoluBL21 (DE3) in LB or M9 medium. Protein samples were purified using Ni-NTA affinity chromatography, FPLC gel filtration (Superdex 75), and/or ionic exchange (MonoQ or MonoS). Isotopic labeling was carried out by expressing the proteins in M9 minimal medium containing 15N-NH4Cl and/or 13C-labeled glucose as the sole source of nitrogen and carbon. Protein complexes were prepared by mixing individual purified domains. Due to the weak binding affinities of DD:RhoGDI and DD homodimer complexes, as well as solubility problems of the DD:CARD complex in salt-containing buffers, gel filtration chromatography could not be used to purify these protein complexes. For the p75NTR DD:RhoGDI complex, two double-labeled samples were prepared in 10 mM D18-HEPES, 10 mM D10-DTT, 1 mM EDTA, and 0.01% sodium azide at pH 6.9: (1) 0.5 mM 13C,15N-labeled p75NTR DD mixed with 2 mM unlabeled RhoGDI; (2) 0.5 mM 13C, 15N-labeled RhoGDI mixed with 2 mM unlabeled p75NTR DD. For the p75NTR DD:RIP2 CARD complex, two double-labeled samples were made in water with 10 mM D10-DTT: (1) 0.5 mM 13C, 15N-labeled p75NTR DD mixed with 1 mM unlabeled RIP2 CARD; (2) 0.5 mM 13C, 15N-labeled RIP2 CARD mixed with 1 mM unlabeled p75NTR DD. For the RIP2 CARD on its own, 0.7 mM 13C, 15N-labeled RIP2 CARD was prepared in 50 mM D10-DTT in water. For the p75NTR DD homodimer, 1 mM 13C, 15N-labeled p75NTR DD was mixed with 1 mM unlabeled p75NTR DD in 50 mM phosphate buffer, 10 mM D10-DTT, 1 mM EDTA and 0.01% sodium azide at pH 6.9.
NMR experiments were performed on a Bruker 800 MHz NMR spectrometer with a cryogenic probe at 28°C. All spectra were processed with NMRPipe (Delaglio et al., 1995) and analyzed with NMRView supported by a NOE assignment plugin (Johnson and Blevins, 1994). Resonance assignments of backbone, aliphatic, and aromatic side chains were obtained using previously described methods (Lin et al., 2006; Xu et al., 2006). Intramolecular NOE restraints were obtained from 4D time-shared 13C, 15N-edited NOESY spectra (Xu et al., 2007). Intermolecular NOEs were identified from 13C,15N-filtered 3D experiments (Zwahlen et al., 1997). Ambiguous NOEs were assigned with iterated structure calculations by DYANA (Herrmann et al., 2002). Final structure calculation was started from 100 conformers. Energy minimization of the 10 conformers with the lowest final target function values was performed in AMBER force field (Case et al., 2002). The mean structure was obtained from the 10 energy-minimized conformers for each domain. PROCHECK-NMR (Laskowski et al., 1996) was used to assess the quality of the structures. All the structural figures were made using MOLMOL (Koradi et al., 1996) or Chimera (Pettersen et al., 2004). The coordinates of p75NTR DD:RhoGDI, RIP2 CARD, p75NTR DD:RIP2 CARD, and p75NTR DD homodimer have been deposited with the Protein Data Bank with PDB IDs 2n80, 2n7z, 2n83, and 2n97, respectively.
The structure of DD:RhoGDI:RhoA was modeled using HADDOCK 2.2 (Dominguez et al., 2003). The starting structures for the trimer were the DD monomeric structure from the lowest-energy structure of p75NTR DD:RhoGDI complex and the crystal structure of human RhoGDI:RhoA(GDP) (PDB ID: 1CC0). The starting structures used to build the hexameric model were the lowest-energy structure of the p75NTR DD homodimer and the crystal structure of human RhoGDI:RhoA(GDP). NOE data between p75NTR DD and RhoGDI were employed to create interaction restraints for both trimer and hexamer. Totally, 1000 rigid-body docking solutions were first generated by energy minimization. The best 100 structures according to intermolecular energies were subjected to semi-flexible simulated annealing in torsion angle space followed by a final refinement in explicit water. Pairwise structure-based alignment and comparison were carried out using a sequential structure alignment program (SSAP) available at the SSAP server (http://www.cathdb.info/cgi-bin/cath/SsapServer.pl).
The apparent hydrodynamic radii of p75NTR DD domain in HEPES or phosphate buffer at pH 7.0 were examined by DLS (DynaPro, Protein Solutions Inc., Lakewood, NJ) at 22°C. The data were analyzed using Dynamics 5.0 software. The CD spectra of all samples were recorded on a Jasco J-810 spectropolarimeter equipped with a thermal controller at 22°C.
All sensorgrams were recorded on a BIAcore T200 at 22°C. For experiments with captured p75NTR DD (ligand), purified p75NTR DD-His was captured onto NTA sensor chips via Ni2+/NTA chelation. Protein samples of purified RIP2 CARD or RhoGDI (analytes) were sequentially diluted in running buffer (10 mM HEPES, 50 mM NaCl, 0.005% Surfactant P20, 0.02% protease-free BSA at pH 7.0) and injected over the surfaces at different concentrations post capture. Regeneration of the NTA surface was performed using 350 mM EDTA. For experiments involving immobilized RhoA, unprenylated RhoA:GDP:Mg2+ was immobilized via amine coupling onto CM5 sensor chips. Unreacted carboxymethyl sites were capped by ethanolamine. Protein samples of analytes were sequentially diluted in running buffer (10 mM HEPES, 50 mM NaCl, 0.005% Surfactant P20, pH 7.0) and injected over the surfaces at different concentrations. To measure the binding of RhoGDI to RhoA, 1 mM MgCl2 and 100 µM GDP were also included in the running buffer. Binding affinities were expressed as equilibrium dissociation constants (Kd) determined by steady state (Figure 2C,D) or kinetic analyses (Figures 4B,C and Figure 3—figure supplement 4B) using the BIA evaluation software. One binding site model was used for fitting of SPR data.
For anisotropy measurements of DD homodimerization, a cDNA encoding Enhanced Green Fluorescent Protein (EGFP) carrying the A206K mutation (to prevent its dimerization) was linked to the C-terminal of the human p75NTR cDNA via DNA ligation and the chimera protein (p75NTR DD-EGFP) was expressed in E. coli BL21(DE3) and purified by FPLC. p75NTR DD-EGFP was sequentially diluted in 50 mM phosphate buffer at pH 7.0. The anisotropy value was obtained from the measurements of fluorescence intensity in both parallel and perpendicular emission modes using a BioTek Cytation Imaging Reader at room temperature. Dimer dissociation constants were obtained by nonlinear fitting of anisotropy measurements to an equation describing a monomer–dimer equilibrium (Martin and Martin, 1996).
Full-length cDNAs encoding human p75NTR, RhoGDI and RIP2 were amplified from human embryonic stem (ES) cell cDNA and subcloned in the pCDNA3 vector backbone (Invitrogen) for protein expression in mammalian cells. Mutations and epitope tags were introduced using QuickChange Site-Directed Mutagenesis Kit (Stratagene, United Kingdom) and verified by DNA sequencing. Normal expression of all constructs was verified by immunoblotting. The origin of antibodies was as follows: ANT-007 anti-p75NTR (for immunoprecipitation) from Alomone Labs; ab52987 anti-p75NTR (for immunoblotting) and anti-RhoGDI from Abcam; anti-Myc from Cell Signaling Technologies; anti-RIP2 from Enzo Life Sciences; anti-β-actin and anti-βIII-tubulin from Sigma-Aldrich. Rip2 knockout mice were obtained from Koichi Kobayashi and Richard Flavell (Kobayashi et al., 2002).
HEK293 and COS-7 cells were obtained from ATCC and cultured under standard conditions in DMEM supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 mg/ml streptomycin, and 2.5 mM glutamine. HEK293 cells were transfected with the polyethylenimine (PEI) method. Briefly, cells were plated in a 10 cm tissue culture dish at a confluency of 3 × 106 cells/dish in normal growth media. Twenty-four hours after plating, the media was changed to growth media containing 1% v/v FBS. Transfection mix was prepared by mixing 1 μg of plasmid with 3 μg of PEI (1 mg/ml) in DMEM. The transfection mix was left to stand at room temperature followed by addition dropwise into culture plates. 24 hrours after transfection, the transfected cells were returned to normal growth media. After a further 24 hr, cells were placed in sera-free media for 16 hr prior to harvest and lysis in 50 mM Tris/HCl pH 7.5, 1 mM EDTA, 270 mM Sucrose, 1% (v/v) Triton X-100, 1 mM benzamidine, 1 mM PMSF, 0.1% (v/v) 2-mercaptoethanol, and in the presence of phosSTOP (Roche) phosphatase inhibitor cocktail mix as per manufacturer instructions. The cellular extracts were then centrifuged at 4°C top speed on a benchtop centrifuge for 15 min. The supernatant was collected and filtered using a 0.2 μM syringe filter. Protein concentration was determined by Bradford Assay. For anisotropy microscopy, COS-7 cells were transfected with Fugene6 (Promega) according to manufacturer’s instructions. For immunoprecipitation, cell extracts (0.5 mg protein) was incubated for 16 hr at 4°C on a rotating wheel with 0.5 μg of anti-p75 antibody (ANT-007, Alomone) attached to Protein G–Sepharose (7.5 μl packed beads). The beads were collected by brief centrifugation (2 min,780× g, 4°C), washed three times with 0.5 ml of Wash Buffer (50 mM Tris/HCl pH 7.5, 1% (v/v) Triton X-100, 0.05% (v/v) 2-mercaptoethanol, and 0.2 M NaCl). After the last wash, pelleted beads were aspirated off the wash buffer followed by addition of Laemmli sample buffer and analysis by SDS-PAGE and Western Blot. Immunoblots were developed using the ECL Western Blotting Kit (Thermo Scientific) and exposed to Kodak X-Omat AR films. Image analysis and quantification of band intensities were done with ImageJ software (NIH). For RhoA activation assays, mouse cerebella were dissected from postnatal day (P) 7 pups. RhoA activity was evaluated in total cerebellar extracts or in lysates of transfected HEK293 cells using the RhoA G-Lisa kit (Cytoskeleton) following the manufacturer’s instructions. Equal amount of protein was used from each sample as determined by Bradford Assay.
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David D GintyReviewing Editor; Howard Hughes Medical Institute, Harvard Medical School, United States
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Thank you for submitting your work entitled "Structural basis of death domain signaling in the p75 neurotrophin receptor" for consideration by eLife. Your article has been favorably evaluated by John Kuriyan (Senior editor) and three reviewers, one of whom, David Ginty, is a member of our Board of Reviewing Editors. The other two referees involved in the review process of your submission have agreed to reveal their identity: Mark Bothwell and Savvas Savvides.
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The contribution by Lin et al. uses 3D NMR to elucidate structures and death domain binding interfaces of components of p75NTR signaling, including the DD domain of p75NTR itself, RhoGDI coupling p75NTR to RhoA activation, and RIP2 coupling p75NTR to NF-kB activation. The structures are consistent with this lab's prior mutational work identifying residues that are important for these binding interactions, and are combined with biophysical and cellular studies to propose a model for p75NTR signaling. Taken together with prior work from this lab, the present study offers, for the first time, a detailed and compelling model for p75NTR signaling. There is substantial additional novelty in the characterization of heterotypic complexes of death domains of different families.
A major concern is that some key mechanistic conclusions in the manuscript are derived from a comparative study of binding affinities of the protein domains, and the mechanistic insights are derived from an SPR-based experimental approach that needs to be revised or at least validated in part by an orthogonal approach.
The strategy to immobilize p75NTR-DD and RhoA via amine coupling onto CM5 sensor chips for the binding studies via SPR introduces uncertainties about the reliability of the data obtained and the ensuing comparison of affinities. This is because the two proteins have lysine residues at or near the interaction interfaces involved in their complexes as revealed by the structural studies. Given that the coupling to CM5 sensor chips would largely occur via surface exposed lysine residues, any ensuing SPR data generated using such an approach is inherently unreliable as partial steric obstruction of available binding sites would compromise the kinetics and affinities of the interaction under study.
The authors appear to possess the necessary tools for readdressing their binding studies, given that they produced the relevant proteins for NMR studies. The Methods section implies, although it does not explicitly state, that recombinant p75NTR-DD and RhoA were produced as His-tagged proteins. If that is indeed the case immobilization approaches described in Wear and Wilkinshaw Analytical Biochemistry 371 (2007) 250-252 might provide a convenient alternative for at least repeating some key measurements. Otherwise, isothermal titration calorimetry might provide yet another tool for generating the necessary orthogonal information on affinities, at least for the strongest interactions in the study. Another approach, albeit experimentally more laborious, would be to produce N- or C-terminally biotinylated versions of the proteins of interest and use streptavidin biosensor chips to immobilize the proteins for SPR measurements.
Minor points (which need to be addressed in the text):
1) Why was RhoA-GDP-Mg2+ used for the binding studies? What are the biological extrapolations from a complex involving RhoA bound by the product of GTP hydrolysis?
2) In the first paragraph of the subsection “Solution structure of the complex between the p75NTR DD and RIP2 CARD”, the authors cite a previous study reporting that the interaction between p75NTR and RIP2 is mediated by their DD and CARD domains, yet, in the first paragraph of the Discussion, the authors indicate that the present study is the first heterotypic interaction described in the DD superfamily. These statements are seemingly in contradiction. Certainly the present statement is the first structural characterization of such a complex.
3) p75NTR is somewhat unusual in that ligand binding terminates a constitutively active signaling pathway (RhoA). The first sentence of the Results indicates that the receptor signals through RhoA constitutively, but fails to make it clear that neurotrophin binding terminates this signaling (although they make this point later). To avoid confusing readers who lack familiarity with the p75NTR system, this point needs to be introduced earlier and more fully (in the Introduction and/or beginning of the Results).
4) The authors mention the wildly oscillatory FRET signal in Figure 6D, but they fail to comment on this further. The authors don't make it clear whether they think reflects a real biological phenomenon, or reflects a technical limitation of measurement of weak FRET signals. Synchronous oscillation of an ensemble of receptors seems implausible, so some comment is necessary.
5) The paper does not mention the other proteins that are recruited to the p75NTR intracellular domain in a neurotrophin-dependent manner (NRIF, TRAF6, etc.). We realize that little can be said at present about how those proteins might influence the death domain interactions described and a full discussion is beyond the scope of the paper, but we would appreciate a discussion of the implication of TRAF6 binding, at least, because of its importance for NF-kB signaling.
6) Data presented in Figure 4B and Figure 4C: What were the concentrations used for each binding curve? It would be convenient for the reader to annotate each SPR binding curve with the corresponding concentrations of the flowed binding partner.
7) Data presented in Figure 2C: Why was this SPR experiment not done in kinetic mode? Please discuss this point.
8) The association 'on-rate' is actually ka and the so-called 'off-rate' should be denoted by kd, with k being a lower case letter and a and d as subscripts. The equilibrium dissociation constant Kd is denoted by a capital K and lower case d in the subscript.
9) Data presented in Figure 3—figure supplement 4 (panel B). What was the concentration series used to obtain the reported kinetic binding data? What binding models did the authors use in SPR data fitting? Please state in each relevant figure legend.
10) Please replace 'sensogram' with 'sensorgram' throughout the manuscript (including the figure legends).
11) It would help the reader tremendously if the authors would come up with a uniform way of annotating the figures presenting binding data to illustrate schematically the geometry of the experiment. For instance, to show which binding partner was immobilized versus the one that was flowed.
12) The authors did not provide (structural) insights as to how the interaction of p75NTR-DD with RhoGDI would lower the affinity between RhoGDI and RhoA. Please discuss this point.
13) A general comment on all figures showing structural models: Please label the N- and C-termini of all models shown.
14) The methods pertaining to the preparation of the recombinant proteins used are not described in sufficient detail. Information on construct design and domain delineation needs to be provided as well as chromatographic elution profiles for the protein complexes used in the structural studies.https://doi.org/10.7554/eLife.11692.022
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
- Carlos F Ibanez
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We thank Chian Ming Low and Yoke Ping Cheong for assistance with FPLC; Jingsong Fan for NMR data collection; Liming Yang for help with SPR measurements; Pin Li for human ES cell cDNA; Koichi Kobayashi and Richard Flavell for Rip2 knockout mice; Peter Verveer, Georgia Xouri, and Philippe Bastiaens for advice on homo-FRET anisotropy; Lilian Kisiswa for preliminary experiments of RhoGDI phosphorylation; and Ket Yin Goh for technical assistance. Support for this research was provided by grants from the National Medical Research Council of Singapore (CBRG13nov012), Ministry of Education of Singapore (MOE2014-T2-1-120), National University of Singapore (Start-Up and Strategic ODPRT Awards), European Research Council (339237-p75ntr), Swedish Research Council (K2012-63X-10908-19-5), Swedish Cancer Society (13-0676) and Knut and Alice Wallenberg Foundation (KAW 2012.0270).
Animal experimentation: Work on Rip2 knock-out mice has been approved by the ethical council of Sweden, ethical protocol number N242/13.
- David D Ginty, Reviewing Editor, Howard Hughes Medical Institute, Harvard Medical School, United States
© 2015, Lin et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.