1. Biochemistry and Chemical Biology
  2. Computational and Systems Biology
Download icon

Synthetic Biology: How to make an oscillator

  1. Bas JHM Rosier
  2. Tom FA de Greef  Is a corresponding author
  1. Eindhoven University of Technology, The Netherlands
Insight
Cite this article as: eLife 2015;4:e12260 doi: 10.7554/eLife.12260
1 figure

Figures

Synthetic gene networks containing three, four and five genes.

The genes in each circuit (top) are translated into protein products, with each protein product repressing the activity of another gene in the network (as indicated by the arrows). Theory predicts that cyclic networks of genes display oscillatory behavior when the number of nodes in the network is odd. Niederholtmeyer et al. found that a circuit consisting of three genes gave rise to well-defined oscillations with a period of up to 8 hr, and that a circuit containing five genes oscillated with a period of 19 hours. In contrast, and in line with theoretical predictions, a network consisting of four nodes did not oscillate: instead it reached a steady state where the activity of all the genes was constant over time.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)