Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions
- Utrecht University, Netherlands
- University of Kent, United Kingdom
- University of Turku, Finland
- University of Utrecht, Netherlands
Abstract
The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. Here, we show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5β and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. We propose that the talin-KANK1 interaction links the two macromolecular assemblies that control cortical attachment of actin fibers and microtubules.
Article and author information
Author details
Reviewing Editor
- W James Nelson, Stanford University, United States
Version history
- Received: May 24, 2016
- Accepted: July 12, 2016
- Accepted Manuscript published: July 13, 2016 (version 1)
- Version of Record published: August 23, 2016 (version 2)
- Version of Record updated: September 27, 2016 (version 3)
Copyright
© 2016, Bouchet et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions
eLife 5:e18124.
https://doi.org/10.7554/eLife.18124
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