1. Developmental Biology and Stem Cells
Download icon

Boundary Dpp promotes growth of medial and lateral regions of the Drosophila wing

  1. Lara Barrio
  2. Marco Milan Is a corresponding author
  1. Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Spain
Research Article
Cited
0
Views
686
Comments
0
Cite as: eLife 2017;6:e22013 doi: 10.7554/eLife.22013

Abstract

The gradient of Decapentaplegic (Dpp) in the Drosophila wing has served as a paradigm to characterize the role of morphogens in regulating patterning. However, the role of this gradient in regulating tissue size is a topic of intense debate as proliferative growth is homogenous. Here we combined the Gal4/UAS system and a temperature-sensitive Gal80 molecule to induce RNAi-mediated depletion of dpp and characterise the spatial and temporal requirement of Dpp in promoting growth. We show that Dpp emanating from the AP compartment boundary is required throughout development to promote growth by regulating cell proliferation and tissue size. Dpp regulates growth and proliferation rates equally in central and lateral regions of the developing wing appendage and reduced levels of Dpp affects similarly the width and length of the resulting wing. We also present evidence supporting the proposal that graded activity of Dpp is not an absolute requirement for wing growth.

Article and author information

Author details

  1. Lara Barrio

    Cell and Developmental Biology, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain
    Competing interests
    The authors declare that no competing interests exist.
  2. Marco Milan

    Cell and Developmental Biology, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain
    For correspondence
    marco.milan@irbbarcelona.org
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-7111-6444

Funding

Ministerio de Economía y Competitividad (BFU2013-44485)

  • Marco Milan

Ministerio de Economía y Competitividad (BFU2016-77587-P)

  • Marco Milan

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Utpal Banerjee, Reviewing Editor, University of California, Los Angeles, United States

Publication history

  1. Received: October 1, 2016
  2. Accepted: June 4, 2017
  3. Accepted Manuscript published: July 4, 2017 (version 1)

Copyright

© 2017, Barrio & Milan

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 686
    Page views
  • 209
    Downloads
  • 0
    Citations

Article citation count generated by polling the highest count across the following sources: PubMed Central, Scopus, Crossref.

Comments

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Computational and Systems Biology
    2. Developmental Biology and Stem Cells
    Ksenia Gnedeva et al.
    Research Article
    1. Neuroscience
    Giles L Colclough et al.
    Research Article