The role of the cellular microenvironment in enabling metazoan tissue genesis remains obscure. Ctenophora has recently emerged as one of the earliest-branching extant animal phyla, providing a unique opportunity to explore the evolutionary role of the cellular microenvironment in tissue genesis. Here, we characterized the extracellular matrix (ECM), with a focus on collagen IV and its variant, spongin short-chain collagens, of non-bilaterian animal phyla. We identified basement membrane (BM) and collagen IV in Ctenophora, and show that the structural and genomic features of collagen IV are homologous to those of non-bilaterian animal phyla and Bilateria. Yet, ctenophore features are more diverse and distinct, expressing up to twenty genes compared to six in vertebrates. Moreover, collagen IV is absent in unicellular sister-groups. Collectively, we conclude that collagen IV and its variant, spongin, are primordial components of the extracellular microenvironment, and as a component of BM, collagen IV enabled the assembly of a fundamental architectural unit for multicellular tissue genesis.https://doi.org/10.7554/eLife.24176.001
The emergence of the diversity of multicellular animals involved cells joining together to form tissues and organs. The ‘glue’ that enabled the cells to work together is made of rope-like molecules called collagen, which assemble into scaffolds. These smart scaffolds tether proteins forming basement membranes that connect cells, provide strength to tissues, and transmit information that influences how the cells behave.
How did collagen evolve over millions of years to enable the ever-increasing complexity, size and diversity of animals? To investigate, Fidler, Darris, Chetyrkin et al. explored the tissues of the most ancient of currently living animals – the comb jellies and sponges. This revealed that among all the collagens that make up the human body, a type called collagen IV was a key innovation that enabled single celled organisms to evolve into multicellular animals. Collagen IV, as molecular glue, enabled the formation of a fundamental architectural unit of basement membrane and cells that allowed multicellular tissues and organs to evolve.
The findings presented by Fidler, Darris, Chetyrkin et al. pose questions about how collagen IV glues cells together, and how information is stored in the rope-like scaffolds to influence cell behavior. Understanding these processes could ultimately lead to the development of new treatments for diseases in which the collagen smart scaffolds play a key role, such as in kidney diseases and cancer.https://doi.org/10.7554/eLife.24176.002
A pivotal event in metazoan evolution was the transition from single-cell organisms to multicellular tissues (Figure 1A). The cellular microenvironment is presumed to play an essential role in this transition, yet the mechanism remains obscure. The basement membrane (BM), a specialized form of extracellular matrix (ECM), is a hallmark morphological feature of the microenvironment of epithelial tissues, and its appearance within the non-bilaterian animal phyla suggests it was a prerequisite (Sherwood, 2015; Hynes, 2012; Ozbek et al., 2010). The BM has numerous functions including maintaining tissue architecture and compartmentalization, organizing growth factor signaling gradients, guiding cell migration and adhesion, delineating apical-basal polarity modulating cell differentiation during development, orchestrating cell behavior in tissue repair after injury, and guiding organ regeneration (Hynes, 2009; Yurchenco, 2011; Vracko, 1974; Pöschl et al., 2004; Daley and Yamada, 2013; Wang et al., 2008; Pastor-Pareja and Xu, 2011; Song and Ott, 2011).
The basement membrane is a supramolecular scaffold, comprised of a toolkit of proteins including collagen IV, laminin, perlecan, and nidogen (Hynes, 2012; Fahey and Degnan, 2010). Among these proteins, recent studies reveal collagen IV is an ancient protein with up to six distinct genes (COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, COL4A6), essential for early development, that functions as a smart scaffold providing tensile strength to tissues, influencing cell behavior by tethering diverse macromolecules, including laminin, proteoglycans, growth factors, binding integrins (Gupta et al., 1997; Bhave et al., 2012; McCall et al., 2014; Fidler et al., 2014; Pöschl et al., 2004; Vanacore et al., 2009; Cummings et al., 2016; Wang et al., 2008; Parkin et al., 2011; Emsley et al., 2000). Disrupting collagen IV scaffolds causes BM destabilization and tissue dysfunction in mice, zebrafish, flies, and nematodes (Pöschl et al., 2004; Fidler et al., 2014; Borchiellini et al., 1996; Gupta et al., 1997). Collectively, these findings reveal that collagen IV, a component of the cellular microenvironment, is essential for tissue architecture and function; yet, the origin and molecular evolution of collagen IV remains obscure.
Knowledge of collagen IV evolution may shed light on the fundamental features of the cellular microenvironment that enabled the transition from single-cell organisms to multicellular tissues. Together, the non-bilaterian animal phyla (Ctenophora, Porifera, Placozoa, and Cnidaria) represent this transition. Importantly, Ctenophora has recently emerged as one of the earliest-branching extant phyla (Ryan et al., 2013; Moroz et al., 2014; Whelan et al., 2015; Telford et al., 2016), along with the sponges (Porifera) (Pisani et al., 2015; Jékely et al., 2015; Telford et al., 2016). Here, we sought to identify ECM components in Ctenophora along with the other non-bilaterian animal phyla, and compared the components to Bilateria and the metazoan sister-groups, Choanozoa, Filasterea, Amoebozoa, and Apusozoa. Our findings reveal that collagen IV and its truncated variant, spongin, are associated with the transition to multicellularity, and further that collagen IV, as a component of BM scaffolds, enabled the genesis of multicellular epithelial tissues.
We characterized the extracellular matrix in Ctenophora (comb jellies) and the other non-bilaterian animal phyla through a combination of immunohistochemistry (IHC), electron microscopy (EM), RNA sequencing, and genomic and transcriptomic analyses. Three ctenophore species, Mnemiopsis leidyi, Beroe ovata, and Pleurobrachia pileus, were used for EM and IHC experiments. Systematic assessment by EM of a number of sections from similar areas in Mnemiopsis was conducted, and no organized basement membrane was encountered. Furthermore, tight junctions between cells and cellular polarization were not observed, both hallmarks of epithelial basement membrane tissue structure (Figure 1B). IHC was congruent with this finding, indicating that collagen IV was dispersed throughout the tissue, surrounding and encompassing cells. In Beroe and Pleurobrachia, however, EM indicated an electron dense layer underlying cells along with cell polarization, and lateral tight junctions between cells, and IHC similarly showed a dense collagen IV layer underlying cells (Figure 1B). Together, these features are congruent with basement membrane architecture and epithelial tissue. Basement membrane structures are prevalent throughout metazoa, from Cnidaria to vertebrates, and we sought to compare the basement membrane architecture in Ctenophora to that of other non-bilaterian animal phyla and Bilateria. Nematostella, along with other cnidarians, have a bilayer body structure composed of endoderm and ectoderm layer with an intervening mesoglea; however, the general BM structure is congruent with that of bilaterian organisms, including mammals. Nematostella demonstrated presence of basement membrane, characterized by polarized cells apical to an electron dense layer by EM, and a concentrated region of collagen IV underlying cell nuclei by IHC (Figure 1C).
We then characterized the ECM composition through analysis of transcriptomic and genomic data across the non-bilaterian animal phyla in comparison with Bilateria and unicellular sister-groups. Ctenophore genomic and transcriptomic data were publicly available from the Pleurobrachia Genome Browser on Neurobase (http://neurobase.rc.ufl.edu/Pleurobrachia) and the Mnemiopsis Genome Project Portal on the National Human Genome Research Institute site (https://kona.nhgri.nih.gov/mnemiopsis/). The ECM components in Nematostella are very similar to that of most bilaterian species BMs (Hynes, 2012), including human, mouse, zebrafish, Drosophila, and C. elegans, consisting of collagen IV, laminin, peroxidasin, collagen XV and XVIII, perlecan, nidogen, fibronectin, as well as spongin (Figure 1C and Figure 2). Ctenophora, however, revealed a simplified set of ECM proteins, with collagen IV and laminin as the only components identified across Beroe, Pleurobrachia, and Mnemiopsis (Figure 1B and Figure 2). Importantly, despite lacking the full gamut of bilaterian ECM proteins, ctenophore cells can still construct a prototypical basement membrane in Pleurobrachia and Beroe. Mnemiopsis, however, does not form a BM despite exhibiting the very same toolkit proteins and this may be a result of secondary loss event.
Across Porifera and Placozoa, basement membranes are uncommon. Placozoa and the poriferan classes of calcareous and demosponges lack basement membranes (Figure 1C) (Ozbek et al., 2010; Leys et al., 2009; Ruthmann et al., 1986; Srivastava et al., 2010), suggesting the BM may have been secondarily lost in these lineages (Cock, 2010) or that it is present only at specific stages during their life cycle (Hynes, 2012). Alternatively, basement membranes may have independently evolved in Ctenophora, Porifera, and Bilateria, a phenomenon that could have occurred because of shared inheritance of ECM proteins and domains from the last common ancestor of the non-bilaterian animal phyla. Investigation of the non-bilaterian animal phyla for components of the BM toolkit suggests that many of the components are present (Srivastava et al., 2010, 2008). Specifically, Placozoa contains the necessary components for a BM, including collagen IV, laminin, perlecan, and nidogen (Srivastava et al., 2008) and also exhibits spongin (Figure 1C and Figure 2) (vide infra). In Porifera, the ECM of homoscleromorph sponges contains basement membranes (Boute et al., 1996), with collagen IV and laminin, but no spongin (Figure 1C and Figure 2). Demosponges, on the other hand, lack any detectable collagen IV and only contain laminin-related domains and spongin. In contrast, the Demosponge and Hexactinellidae classes of Porifera, which both lack collagen IV, do not have basement membranes (Figure 1C) (Adams et al., 2010).
Laminin architecture appears to be present in choanoflagellates (Fahey and Degnan, 2012), and additionally laminin-related genes appear in all other unicellular species including Capsaspora owczarzaki, Dictyostelium discoideum, and Thecamonas trahens (Figure 2). Although no other complete ECM components exist in unicellular choanoflagellates (King, 2005), several domains and fragments of ECM components have been identified, including collagenous repeats, laminin G (globular) domains and LN (N-terminal) domains, and fibronectin type II and III domains (Figure 1D) (King et al., 2008). Furthermore, no other complete ECM components exist in other unicellular organisms, including Salpingoeca rosetta (Choanozoa), Capsaspora owczarzaki (Filasterea), Dictyostelium discoideum (Amoebozoa), or Thecamonas trahens (Apusozoa); however, collagenous GXY repeats were identified in Monosiga, Salpingoeca, and Dictyostelium. Receptors for collagen binding, including integrin, dystrophin, and Discoidin domain receptors (DDRs) were analyzed across metazoa and unicellular protists (Figure 2). Integrins are highly conserved across metazoa and are found in the unicellular Capsaspora and Thecamonas. Dystrophin was identified in humans, fruit fly, Nematostella, Amphimedon, as well as in unicellular protists but is absent in Trichoplax, Oscarella, and ctenophores. DDR 1 and 2 was identified only in humans and fruit fly. Collectively, the ECM composition across the non-bilaterian animal phyla points to laminin and collagen IV as highly conserved components, and importantly, that they are associated with BM and epithelial tissue architecture.
We characterized the gene and protein structure of collagen IV in Ctenophora and the other non-bilaterian animal phyla. In Mnemiopsis, 11 full-length collagen IV genes were discovered, which contrasts with the two genes typically present throughout invertebrates and the six genes typically found in vertebrates (Khoshnoodi et al., 2008). The head-to-head orientation is a distinguishing characteristic of collagen IV genes among other collagens of Bilateria (Kaytes et al., 1988; Hudson et al., 1993). In Mnemiopsis, four genes occur on the same scaffold and exhibit a head-to-head orientation (ML17501a and ML17504a, ML17502a and ML17503a) (designated as Group I) (Figure 3A). Furthermore, ML17502a and ML17503a genes are oriented in opposite directions, separated by ~2879 bases, and do not share the same promoter. The other seven Mnemiopsis genes: ML166441a, ML18175a, ML18197a, ML18198a, ML034334a, ML0343336a, and ML0343337a (designated as Group II), are aligned individually on separate scaffolds or in a unidirectional tandem array. Transcriptomic analysis of adult Mnemiopsis reveals differential expression within each group of collagen IV genes. In Group I, the expression of ML17501a and ML17502a is significantly higher than other Group I genes. Similarly, ML034334a, ML0343336a, and ML0343337a show considerable increase in expression levels compared to the other Group II genes (Figure 3B).
Mnemiopsis collagen IV genes are shorter in sequence length and typically have fewer exons and shorter intronic regions compared to their Bilaterian counterparts (Figure 3C). Whereas bilaterian fibrillar collagen genes are composed of multiple exons with a length of 54 base pairs (Yamada et al., 1980), exons of Mnemiopsis collagen IV genes range in size from 40 to 4867 bp. Among the Mnemiopsis genes, exons of 108 bp in length were found in several genes, including ML17503a, ML17504a, and ML16441a, but no increased frequency of 54 bp exons in length or any variation was detected) (Figure 3—figure supplement 1). The presence of split glycine codons coding for the collagenous domain and codons on junctional exons (e.g. collagenous domain/NC1 domain encoding exons) are defining features of vertebrate collagen IV genes (Quinones et al., 1992). Multiple genes from each group (Group I: ML17501a, ML17502a, and ML17503a; Group II: ML034334a, ML034336a, and ML034337a) possess split glycine codons on the collagenous domain/NC1 domain junction exon (Figure 3—figure supplement 2 and Supplementary file 1).
We then determined the number of collagen IV genes in 10 other species from the Ctenophora phylum. Transcriptome analysis was conducted using in-house generated libraries for Mnemiopsis leidyi and Pleurobrachia pileus and publicly available libraries for Pleurobrachia bachei, Beroe ovata, Beroe abyssicola, Euplokamis sp., Dryodora sp., Vallicula sp., Coeloplana sp., and Bolinopsis sp. Across these ten species, a total of 118 unique collagen IV genes were detected. Each species contained a variable number of collagen IV genes, ranging between 4 and 20 genes each, as compared to the 2 to 6 genes in the other non-bilaterian animal phyla and Bilateria (Figure 4A and B). All species, apart from the two Beroe species surveyed, contain a combination of both Group I and II chains, with the two Beroe species exhibiting only Group II chains. In addition to full-length collagen IV genes, two genes, with signal peptides, encoding only the NC1 domain were identified across ten species of Ctenophora. Expression of NC1 domains without collagenous tails is novel (Figure 4C). Together, these findings show that the ECM of ctenophores contain both collagen IV and standalone NC1 genes, and that the number and diversity collagen IV genes exceeds that of any other metazoan group.
We also determined the number, structure, and orientation of collagen IV genes in Nematostella vectensis (Cnidaria) and Trichoplax adhaerens (Placozoa). We found two genes in both species, and that they are homologous with Bilateria, with each demonstrating a ‘head-to-head’ orientation and homologous coding regions for both collagenous and non-collagenous domains (Figure 3D). Complete genomic data was unavailable to determine the orientation of the two collagen IV genes in homoscleromorph sponges. In contrast, unicellular protists (Choanozoa, Filasterea, Amoebozoa, Apusozoa) do not contain collagen IV as determined by genomic analyses (Figure 2). Together, our findings show that head-to-head orientation of collagen IV is conserved across Bilateria, Cnidaria, Placozoa, and Ctenophora, whereas Ctenophora exhibits both head-to-head and tandem orientations (Figure 3A).
Several prominent structural domains characterize Bilaterian collagen IV chains (Figure 5). These include an N-terminal non-collagenous domain rich in cysteine and lysine residues (NC3) (Figure 5—figure supplement 1), a large collagenous domain of Gly-Xaa-Yaa (GXY) repeats of ~1400 residues with interruptions in the GXY repeats (Figure 6), followed by a non-collagenous (NC1) domain at the C-terminus of approximately ~230 residues (Figure 5—figure supplement 2). NC1 domains are comprised of two C4 domains, each containing a short, highly conserved HSQ residue motif proximal to the N-terminal side. We sought to determine whether these structural domains are characteristic of Ctenophora and the other non-bilaterian animal phyla. Indeed, these domains are conserved across Cnidaria, Placozoa, Porifera, and Ctenophora (Figure 5). The NC1 domain also contains a chloride-binding motif, which functions in binding extracellular chloride to signal the assembly of collagen IV networks and is conserved from vertebrates to Cnidaria and Placozoa (Cummings et al., 2016). The chloride-binding motif was identified in both Ctenophora (group II chains) as well as in the two Homoscleromorph sponges analyzed, suggesting the chloride signaling function of NC1 domains is also conserved in Ctenophora and Porifera (Figure 5—figure supplement 3). Together, these findings reveal that the conserved structural features of bilaterian collagen IV extend across the non-bilaterian animal phyla, including Ctenophora.
The NC1 domain is the molecular recognition module that directs the assembly of collagen IV protomers and networks (Cummings et al., 2016). NC1 modules function in selecting collagen IV chains for trimerization, forming triple-helical protomers, and for oligomerization of protomers into networks (Figure 5) (Cummings et al., 2016; Khoshnoodi et al., 2008). The NC1 modules are stabilized by sulfilimine cross-links, which connect methionine-93 (Met-93) and lysine/hydroxylysine-211 (Lys/Hyl-211) between adjoining protomers (Fidler et al., 2014; Vanacore et al., 2009). Sulfilimine cross-links are conserved throughout Bilateria, from Humans to C. elegans, and in Cnidaria, with the exception of Hydra (Fidler et al., 2014). In contrast, this cross-link is absent in Ctenophora, owing to the absence of Met-93 and Lys/Hyl-211 residues (Figure 5—figure supplement 4). Thus, we sought to characterize the biochemical properties of ctenophore NC1 domains to ascertain whether they are stabilized by an alternative cross-linking mechanism. Uniquely, ctenophore collagen IV is distinguished from all other metazoans by the presence of two additional domains. One is a non-collagenous domain (NC2 domain) that is approximately 38–44 residues in length within the collagenous domain (Figure 5—figure supplement 5). The other domain is 11–13 residues in length and consists of 3–4 conserved cysteine residues, designated as the cysteine-loop, which is an extension of the canonical NC1 domain, and a candidate for an alternative cross-linking mechanism (Figure 5—figure supplement 6). As we previously established for bilaterian collagen IV, the presence of NC1 dimers after reduction of with mercaptoethanol, indicates cross-links (Fidler et al., 2014; Vanacore et al., 2009). Analysis in the three ctenophore species, Mnemiopsis, Beroe and Pleurobrachia, by SDS-PAGE and gel filtration chromatography, revealed the presence of NC1 hexamers, which upon reduction dissociated into dimers (Figure 7A–D). Since the dimers lack Met-93 and Lys/Hyl-211, the results indicate that ctenophore dimers are stabilized by the cysteine-loop (Figure 7E). Hence, the cross-linking mechanism of ctenophores (cysteine-loop) is distinguished from that of Cnidaria and Bilateria (sulfilimine cross-links).
NC1 domains are distinguishing domains of collagen IV that function as recognition modules in the assembly of collagen IV networks (Cummings et al., 2016). Uniquely, all 10 ctenophore species possess a gene encoding only the NC1 domain, a feature not found in any other phyla. We performed phylogenetic analysis to compare the NC1 domains of non-bilaterian animal phyla with that of Bilateria (Figure 8). The results placed the NC1 domain of ctenophore collagen IV into two major groups, which are consistent with genomic orientation of Group I and Group II (vida supra). We conducted additional phylogenetic analysis of the NC1 domain using RAxML to select the best tree from eleven models of evolution (DAYHOFF, DCMUT, JTT, MTREV, WAG, RTREV, CPREV, VT, BLOSUM62, MTMAM, and LG). Among these, the VT model yielded the tree with the best-fit (Figure 8—figure supplement 1). The distinction of the two groups coincides with the presence of the novel structural domains, NC2 and cysteine-loop, found exclusively in ctenophore Group II chains. Furthermore, these groups can be further subdivided into subgroups Group I (A-E) and Group II (A-D) based on phylogenetic affinity (Figure 8—figure supplement 1). RAxML phylogenetic analysis revealed a closer affinity between the ctenophore NC1 genes and collagen IV NC1 domain from the non-bilaterian animal phyla and Bilateria, as compared to ctenophore Group I and II chains (Figure 8). Furthermore, Group I and Group II ctenophore chains showed a much higher rate of divergence both within the phylum and as compared to the other non-bilaterian animal phyla and bilaterian collagen IV sequences. The unrooted tree topology illustrates the high phylogenetic affinity between ctenophore NC1 proteins and bilaterian NC1 domain that cluster closely.
Spongins are a family of collagen IV-related proteins composed of a short collagenous domain attached to an NC1 domain. This protein family was first detected in the exoskeleton of demosponges and has been subsequently identified in cnidarians, across invertebrates (with the exception of ecdysozoans, e.g., C. elegans, Drosophila), and in basal chordates (Aouacheria et al., 2006; Exposito et al., 1991). Interestingly, spongins do not occur in vertebrates (Aouacheria et al., 2006), and we did not detect them in Ctenophora. We examined the phylogenetic relationship of the NC1 domain of spongins to that of collagen IV NC1 domains (Figure 8 and Figure 8—figure supplement 1). Multiple sequence alignment showed conservation of seven cysteine residues between spongins and collagen IV, while the HSQ motif was absent in spongin sequences (Figure 9). Comparison of collagen IV sequences revealed four cysteine residues that are absent in spongin sequences. The spongin variants, however, do show conservation of three cysteines that are absent in collagen IV sequences. Collectively, the presence of collagenous domains and the conservation of collagen IV NC1 domain features within spongin NC1 domains, reveal that they are homologous to collagen IV protein domain structure, as previously noted (Exposito et al., 1991).
Within Ctenophora, collagen IV underwent numerous gene duplication events resulting in an unprecedented diversity in both gene sequence and organization in comparison to all other metazoans. Ctenophora contains between 4 and 20 collagen IV chains across species, and exhibits both head-to-head orientation and genes aligned individually on separate scaffolds or in a unidirectional tandem array. Interestingly, Ctenophora has both collagen IV genes and a standalone NC1 gene. In both Nematostella and Trichoplax, there are two collagen IV genes exhibiting head-to-head gene orientation, similar to that of Bilateria. In Porifera, the ECM of homoscleromorphs is composed of two collagen IV genes, while in demosponges, it is composed of spongin, a collagen IV variant. Spongin is absent in Ctenophora but is present in non-bilaterian animal phyla, invertebrates, and lower chordates along with collagen IV throughout invertebrates and lower chordates, with the exception of Drosophila and C. elegans. Structural and phylogenetic analysis of spongin shows it is homologous to collagen IV (Figures 8 and 9). Collectively, collagen IV genes are highly conserved across the non-bilaterian animal phyla and Bilateria; yet, in Ctenophora, these genes are more diverse and distinct including a novel cross-linking mechanism, with up to 20 distinct genes compared with six in vertebrates. Moreover, the collagen IV gene is absent in the unicellular sister-groups (choanoflagellates, filastereans, amoebozoans, and apusozoans), suggesting it was an early metazoan innovation.
To address the evolutionary origin of the collagen IV gene, we compared two scenarios, Ctenophora-first versus Porifera-first (Ryan et al., 2013; Moroz et al., 2014; Whelan et al., 2015; Telford et al., 2016). In both scenarios, collagen IV appeared in an early metazoan ancestor or a unicellular ancestor but was secondarily lost in demosponges and hexactinellid sponges (Figure 10A and B). It is noteworthy that the NC1 gene is present only in Ctenophora (Figure 10C and D), suggesting that this gene is a remnant from an early metazoan ancestor, and a forerunner to the NC1 domain of the ancestral collagen IV gene. This NC1 gene encodes a key recognition module that directs the assembly of collagen IV suprastructures (Cummings et al., 2016). In comparison, spongin appeared after the divergence of the Ctenophora phylum in the Ctenophora-first hypothesis, yet in the Porifera-first hypothesis it would have appeared alongside collagen IV in the early metazoan ancestor or unicellular ancestor. With either hypothesis, the collagen IV gene coincided with the appearance of multicellular animals. Although laminin genes appear to have arisen prior to the metazoan lineage, with laminin-related genes appearing in unicellular choanoflagellates (Fahey and Degnan, 2012) (Figure 10A and B).
Collectively, we propose a model for collagen IV gene evolution that incorporates both Ctenophora-first and Porifera-first hypotheses (Figure 11). The presence of Gly-X-Y collagenous repeats, in the absence of a collagen IV gene, in choanoflagellates and amoebozoa, and the presence of a NC1 gene in the Ctenophora phylum suggests that Gly-X-Y repeats combined with an NC1 domain gene in an early metazoan ancestor, or possibly in a unicellular ancestor, forming an ancestral collagen IV gene. This combination of domains is analogous to the domain shuffling events that gave rise to the developmental protein, hedgehog (Adamska et al., 2007). Within Ctenophora, collagen IV genes underwent unprecedented experimentation with several duplication events resulting in up to twenty distinct genes with both tandem and head-to-head organization. Within Porifera, the collagen IV gene was duplicated with a head-to-head orientation. This head-to-head feature was conserved in both sequence and gene structure throughout non-bilaterian animals and Bilateria (Figure 11), with the known exception of C. elegans (Guo and Kramer, 1989). Two additional rounds of genome duplication resulted in six collagen IV genes in the vertebrate subphylum. Spongin, a collagen IV variant, first appeared in Porifera and is conserved throughout invertebrates, with the exception of Ecdysozoa and is found in cephalochordates (Branchiostoma floridae) and tunicates (Ciona intestinalis). The spongin gene arose either by domain shuffling of Gly-X-Y repeats from a unicellular ancestor and the NC1 gene, analogous to the assembly of the ancestral collagen IV gene, or diverged from an ancestral collagen IV gene (Figure 11).
Collagen IV protein, or its spongin variant, is a required ECM component for all extant multicellular animals, considering that all animals investigated contain either collagen IV or spongin, and that the essentiality of collagen IV during development has been established in several studies (Gupta et al., 1997; Pöschl et al., 2004; Gotenstein et al., 2010; Bhave et al., 2012). The collagen IV protein is associated with two distinct organizations of cells; one in which the ECM contains collagen IV broadly dispersed between communities of cells (Mnemiopsis) and the other in which collagen IV is a component of a well-defined BM underlying a layer of cells (Beroe, Pleurobrachia, Homoscleromorph sponges, and Nematostella), a hallmark feature of epithelial bilaterian tissues (Figure 12). The absence of BMs in Trichoplax and Mnemiopsis suggests there is an unknown component that facilitates the assembly of collagen IV and laminin into a basement membrane.
Collectively, we conclude that collagen IV and its spongin variant are primordial components of the extracellular microenvironment, and collagen IV, as a component of BM, enabled the assembly of a fundamental architectural unit for the genesis and evolution of multicellular tissues (Figure 12). This unit is characterized by a layer of apical/basal-polarized cells that are laterally connected by tight junctions between plasma membranes, which are basally anchored via integrin receptors embedded in plasma membranes to a basement membrane supra-scaffold. In turn, this architectural unit served as the building block that enabled the formation and evolution of epithelial tissues, the ever-increasing complexity and size of organisms, and for the expansion and diversity of the animal kingdom.
Transcriptomes used in this study were sequenced at the Vanderbilt Technologies for Advanced Genomics Core Facility (VANTAGE, Nashville, TN). The Illumina TruSeq mRNA Sample Preparation Kit was used to convert the mRNA in 100 ng of total RNA into a library of template molecules suitable for subsequent cluster generation and sequencing on the Illumina HiSeq 2500 using the rapid run setting. The pipeline established in VANTAGE was followed and is briefly described below. The first step was a quality check of the input total RNA by running an aliquot on the Agilent Bioanalyzer to confirm RNA integrity. The Qubit RNA fluorometry assay was used to measure sample concentrations. The input-to-library prep was 100 ng of total RNA (2 ng/ul). The poly-A containing mRNA molecules were concentrated using poly-T oligo-attached magnetic beads. Following purification, the eluted poly(A) RNA was cleaved into small fragments of 120–210 base pair (bp) using divalent cations under elevated temperature. The cleaved RNA fragments were copied into first strand cDNA using SuperScript II reverse transcriptase and random primers. This step was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H treatment. The cDNA fragments then went through an end repair process, the addition of a single ‘A’ base, and then ligation of the Illumina multiplexing adapters. The products were then purified and enriched with PCR to create the final cDNA sequencing library. The cDNA library then undergoes quality control by running on the Agilent Bioanalyzer HS DNA assay to confirm the final library size and on the Agilent Mx3005P qPCR machine using the KAPA Illumina library quantification kit to determine concentration. A 2 nM stock was created and samples pooled by molarity for multiplexing. From the pool, 12 pmoles were loaded into each well for the flow cell on the Illumina cBot for cluster generation. The flow cell was then loaded onto the Illumina HiSeq 2500 utilizing v3 chemistry and HTA 1.8. The raw sequencing reads were processed through CASAVA-1.8.2 for FASTQ conversion and demultiplexing. The Illumina chastity filter was used and only the PF (passfilter) reads are retained for further analysis. Assembly of transcriptomes was performed using both Velvet/Oases and Trinity software packages with default settings (see list of commands subsection below).
List of commands used in sequence search:
velveth $outDir $hash_length -fastq -shortPaired $in_shuffled_ sequence_file
velvetg $outDir -read_trkg yes
oases $outDir -ins_length 150
Trinity.pl –output $outDir –seqType fq –JM 90G –left $file1 –right $file2 –CPU 16
Animals were initially fixed whole in cold 2.5% glutaraldehyde in 0.1M cacodylate buffer, pH7.4 overnight in the refrigerator. After this initial fixation, the samples were stable enough so that small portions of selected areas could be dissected out and fixed for a further 24 hr at 4°C in 2.5% glutaraldehyde in 0.1M cacodylate. Following fixation, the samples were washed in 0.1M cacodylate buffer, incubated 1 hr in 1% osmium tetroxide at RT then washed with 0.1M cacodylate buffer, dehydrated through a graded ethanol series and embedded in epoxy resin. Semi-thin sections (0.5 microns) were cut, stained with toluidine blue and viewed by light microscopy to choose appropriate areas for study. Thin sections (70–80 nm) were cut from these selected areas and contrasted using 2% uranyl acetate and Reynold’s lead citrate, and imaged on an FEI Tecnai T12 electron microscope.
Whole ctenophore tissues were frozen in liquid nitrogen, pulverized in a mortar and pestle and then homogenized in 2.0 ml g−1 digestion buffer and 0.1 mg ml−1 Worthington Biochemical bacterial collagenase and allowed to digest at 37°C, with spinning for 24 hr. Liquid chromatography purification of solubilized NC1 varied by species based on protein yield. All ctenophore NC1s were purified by gel-exclusion chromatography (GE Superdex 200 10/300 GL). For reduction and alkylation of collagen IV NC1 hexamers, fractions containing high-molecular-weight complex from size-exclusion chromatography were concentrated by ultrafiltration and reduced in TBS buffer with various concentrations of DTT. After incubation for 30 min at 37°C, samples were alkylated with twofold molar excess of iodoacetamide for 30 min at room temperature in the dark. After mixing with SDS loading buffer, samples were heated for 5 min in boiling water bath and analyzed by non-reducing SDS-PAGE. Collagenase-solubilized NC1 hexamers were analyzed by SDS-PAGE in 12% bis-acrylamide mini-cells with Tris-Glycine-SDS running buffer. Sample buffer was 62.5 mM Tris-HCl, pH 6.8, 2% SDS (w/v), 25% glycerol (w/v), 0.01% bromophenol blue (w/v). Western blotting of SDS-dissociated NC1 hexamer was developed with JK-2, rat monoclonal antibody (kindly provided by Dr. Yoshikazu Sado, Shigei Medical Research Institute, Okayama, Japan). All Western blotting in Figure 6 was done with Thermo-Scientific SuperSignal West Femto chemiluminescent substrate and digitally imaged with a Bio-Rad GelDoc.
Whole ctenophore tissues were placed in 150 mL beaker and as much liquid was removed as possible. Each tube with tissue was filled with 100 mL ice-cold ctenophore fixation buffer 1 [80ul Glutaraldehyde (25%), 0.02% final concentration; 25 mL Paraformaldehyde (16%), 4.0% final concentration; 75 mL 0.2um-filtered seawater (Red Sea Coral Pro Salt)], inverted a few times gently, and left at 4 degrees Celsius for 5 min. Buffer 1 was then removed, and 100 mL of ctenophore fixation buffer 2 was added [25 mL Paraformaldehyde (16%), 4.0% final concentration; 75 mL 0.2um-filtered seawater (Red Sea Coral Pro Salt)]. Buffer 2 was then removed and tissues were gently washed five times with cold 1X PBS. Fixation protocol adopted from Pang and Martindale, Ctenophore Whole-Mount Antibody Staining (Pang and Martindale, 2008). Tissues were then embedded in parafilm and sectioned onto individual slides for IHC staining. After deparaffinization and rehydration, tissues underwent heat-induced epitope retrieval with DAKO and microwaved for 15 min. Cold tap water was then run over tissues for 10 min, followed by two washed with 1X PBS and stored in 1X PBS. Immunostaining occurred at room temperature, with blocking by a 5% serum blocking buffer (1X PBS pH 7.4/5% normal goat serum/0.1% Triton X-100) for 60 min. All IHC for collagen IV was conducted with the rat monoclonal antibody (mAb) JK-2, and antibody dilution was done in 5% serum blocking buffer accordingly. Alexa488 tagged anti-rat secondary was used for the fluorochrome-conjugated secondary antibody (RRID:AB_10893331), and dilution was also done in 5% serum blocking buffer. IHC images were taken on a Zeiss Axioplan microscope. Lenses used were a 20X lens (Plan-APOCHROMAT 20X/0,75; ∞/0,17) and a 40X lens (Plan-NEOFLUAR 40X/0,75; ∞/0,17). Images were taken at room temperature, approximately 20 degrees Celsius. All images were done in an imaging medium of air. Fluorochromes used were Alexa488 (green) for collagen IV, and Hoescht stain (blue) was used for nuclei staining. The Camera for imaging was a Photometrics CoolSnap HQ, using Metamorph 22.214.171.124 software (RRID:SCR_002368). Slight gamma correction of (< ± 0.2) after acquisition to adjust contrast. Images captured were merged with ImageJ64, 1.48v (RRID:SCR_003070).
The evolutionary relationship between collagen IV and spongins was analyzed using the NC1 domains of each of the 139 sequences in our dataset. NC1 domains were aligned using the Geneious alignment tool within Geneious software package (RRID:SCR_010519), version 8.1.9 with default settings (Silvestro and Michalak, 2012). The resulting sequence alignment, which was 881 amino acid sites in length, was used to reconstruct the phylogeny of NC1 domains under the maximum likelihood optimality criterion as implemented in the RAxML software (RRID:SCR_006086), version 8.2.3 (Stamatakis, 2014). The phylogenetic analysis was performed using the PROTGAMMAAUTO option, which selects the substitution model with the best fit to the alignment among a set of among a set of 11 models (these were: DAYHOFF, DCMUT, JTT, MTREV, WAG, RTREV, CPREV, VT, BLOSUM62, MTMAM, and LG). In the case of the NC1 domain phylogeny, the model with the best fit was the VT model (Müller and Vingron, 2000). Robustness in phylogeny inference was assessed with 100 bootstrap replicates.
Insights into early extracellular matrix evolution: spongin short chain collagen-related proteins are homologous to basement membrane type IV collagens and form a novel family widely distributed in invertebratesMolecular Biology and Evolution 23:2288–2302.https://doi.org/10.1093/molbev/msl100
Peroxidasin forms sulfilimine chemical bonds using hypohalous acids in tissue genesisNature Chemical Biology 8:784–790.https://doi.org/10.1038/nchembio.1038
The function of type IV collagen during Drosophila muscle developmentMechanisms of Development 58:179–191.https://doi.org/10.1016/S0925-4773(96)00574-6
Extracellular chloride signals collagen IV network assembly during basement membrane formationThe Journal of Cell Biology 213:479–494.https://doi.org/10.1083/jcb.201510065
ECM-modulated cellular dynamics as a driving force for tissue morphogenesisCurrent Opinion in Genetics & Development 23:408–414.https://doi.org/10.1016/j.gde.2013.05.005
Origin of animal epithelia: insights from the sponge genomeEvolution & Development 12:601–617.https://doi.org/10.1111/j.1525-142X.2010.00445.x
Origin and evolution of laminin gene family diversityMolecular Biology and Evolution 29:1823–1836.https://doi.org/10.1093/molbev/mss060
Mapping structural landmarks, ligand binding sites, and missense mutations to the collagen IV heterotrimers predicts major functional domains, novel interactions, and variation in phenotypes in inherited diseases affecting basement membranesHuman Mutation 32:127–143.https://doi.org/10.1002/humu.21401
A developmental biologist's "outside-the-cell" thinkingThe Journal of Cell Biology 210:369–372.https://doi.org/10.1083/jcb.201501083
Organ engineering based on decellularized matrix scaffoldsTrends in Molecular Medicine 17:424–432.https://doi.org/10.1016/j.molmed.2011.03.005
Basement membranes: cell scaffoldings and signaling platformsCold Spring Harbor Perspectives in Biology 3:a004911.https://doi.org/10.1101/cshperspect.a004911
Harry C DietzReviewing Editor; Howard Hughes Medical Institute and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, United States
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your article "Collagen IV and the evolutionary dawn of metazoan tissues" for consideration by eLife. Your manuscript has now been reviewed by two reviewers and a Senior Editor. The following individuals involved in review of your submission have agreed to reveal their identity: Kevin P Campbell (Reviewer #2).
While there was general enthusiasm about this study, there are a number of issues that require attention, with the need for submission of a fully responsive revised manuscript that will again be evaluated by the reviewers.
One shared opinion relates to the choice and limited number of organisms that were assessed for collagen IV. Were other eukaryotes analyzed? Why was only one choanoflagellate checked? There are two genomes available: Monosiga brevicollis and Salpingoeca rosetta that should be included. The list could extend to opisthokonts (such as Capsaspora owczarzaki), as well as Amoebozoa and Apusomonadida (Thecamonas trahens). Along similar lines, it is interesting that you did not see an organized basement membrane in Mnemiopsis even though collagen IV and laminin are expressed. One possible explanation for this result is that Mnemiopsis lacks a critical collagen IV or other basement membrane receptor. If there are any data on the expression of various basement membrane receptors in Mnemiopsis, Beroe and Pleurobrachia it would be good to include this information in the manuscript.
It would be useful to provide a table with the gene content related to ECM in each of the taxa analyzed. There was also a request for greater explanation of phylogenetic methods that were employed in the study. How were sequences aligned and how many amino acid positions were included at the end? The bootstrap is explained, but how did you select the best, final tree? Which model of evolution was used? Was the LG model of evolution assessed? The trees shown in the figures are without branch lengths without explanation?
There was also an opinion that you should consider alternative evolutionary scenarios for the origin of collagen IV and the possibility that sponges diverged prior to Ctenophora and Bilateria. What would that imply?https://doi.org/10.7554/eLife.24176.028
- Billy G Hudson
- Antonis Rokas
- Antonis Rokas
- Julie K Hudson
- Billy G Hudson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
The technical work of Neonila Danylevych is greatly appreciated. We thank Carl Luer and the Mote Marine Laboratory for assistance with Mnemiopsis field collections. We acknowledge the Vanderbilt Technologies for Advanced Genomics (VANTAGE) for technical work in transcriptome assemblies. Electron microscopy was carried out in part through the use of the VUMC Cell Imaging Shared Resource (supported by NIH grants CA68485, DK20593, DK58404, DK59637 and EY08126). We would like to thank Dr. Yoshikazu Sado (Shigei Medical Research Institute, Okayama, Japan) for kindly providing the collagen IV JK-2 monoclonal antibody. This work counts in part towards the doctoral dissertation of ALF. at Tennessee State University. The authors declare no competing financial interests.
- Harry C Dietz, Reviewing Editor, Howard Hughes Medical Institute and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, United States
- Received: December 13, 2016
- Accepted: March 23, 2017
- Version of Record published: April 18, 2017 (version 1)
© 2017, Fidler et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.