1. Genes and Chromosomes
  2. Plant Biology
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A quantitative hypermorphic CNGC allele confers ectopic calcium flux and impairs cellular development

  1. David M Chiasson
  2. Kristina Haage
  3. Katharina Sollweck
  4. Andreas Brachmann
  5. Petra Dietrich
  6. Martin Parniske Is a corresponding author
  1. Ludwig Maximilian University of Munich, Germany
  2. Friedrich-Alexander Universität Erlangen-Nürnberg, Germany
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Cite as: eLife 2017;6:e25012 doi: 10.7554/eLife.25012

Abstract

The coordinated control of Ca2+ signaling is essential for development in eukaryotes. Cyclic nucleotide-gated channel (CNGC) family members mediate Ca2+ influx from cellular stores in plants1-4. Here we report the unusual genetic behavior of a quantitative gain-of-function CNGC mutation (brush) in Lotus japonicus resulting in a leaky tetrameric channel. brush resides in a cluster of redundant CNGCs encoding subunits which resemble metazoan voltage-gated potassium (Kv1-Kv4) channels in assembly and gating properties. Plants homozygous for brush are impaired in root development and infection by nitrogen-fixing rhizobia which segregated as a recessive monogenic trait. The brush allele exhibited quantitative behavior since overexpression of the cluster subunits was required to suppress the brush phenotype. The results reveal a mechanism by which quantitative competition between channel subunits for tetramer assembly can impact the phenotype of the mutation carrier.

Article and author information

Author details

  1. David M Chiasson

    Faculty of Biology, Genetics, Ludwig Maximilian University of Munich, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-0770-2684
  2. Kristina Haage

    Faculty of Biology, Genetics, Ludwig Maximilian University of Munich, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Katharina Sollweck

    Faculty of Biology, Genetics, Ludwig Maximilian University of Munich, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
  4. Andreas Brachmann

    Faculty of Biology, Genetics, Ludwig Maximilian University of Munich, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
  5. Petra Dietrich

    Division of Molecular Plant Physiology, Department of Biology, Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany
    Competing interests
    The authors declare that no competing interests exist.
  6. Martin Parniske

    Faculty of Biology, Genetics, Ludwig Maximilian University of Munich, Martinsried, Germany
    For correspondence
    parniske@lmu.de
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0001-8561-747X

Funding

Deutsche Forschungsgemeinschaft (FOR964 (Calcium))

  • Kristina Haage
  • Petra Dietrich
  • Martin Parniske

H2020 European Research Council (340904 (EvolvingNodules))

  • Martin Parniske

Alexander von Humboldt-Stiftung

  • David M Chiasson

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Maria J Harrison, Reviewing Editor, Boyce Thompson Institute for Plant Research, United States

Publication history

  1. Received: January 10, 2017
  2. Accepted: September 20, 2017
  3. Accepted Manuscript published: September 21, 2017 (version 1)
  4. Version of Record published: December 5, 2017 (version 2)

Copyright

© 2017, Chiasson et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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