A tunable refractive index matching medium for live imaging cells, tissues and model organisms

Abstract
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Article and author information
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Abstract

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.

Data availability

The following data sets were generated

1. Boothe T
2. Hilbert L
3. Heide M
4. Berninger L
5. Zaburdaev V
6. Vastenhouw N
7. Huttner W
8. Myers G
9. Drechsel D
10. Rink J.
(2017) Data from: A tunable refractive index matching medium for live imaging cells, tissues and model organisms
Available at Dryad Digital Repository under a CC0 Public Domain Dedication.

https://doi.org/10.5061/dryad.83gp7

Article and author information

Author details

Tobias Boothe

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Lennart Hilbert

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Michael Heide

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Lea Berninger

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Wieland B Huttner

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-4143-7201
Vasily Zaburdaev

Max Planck Institute for the Physics of Complex Systems, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Nadine L Vastenhouw

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-8782-9775
Eugene W Myers

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
David N Drechsel

Research Institute of Molecular Pathology, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Jochen C Rink

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

For correspondence
rink@mpi-cbg.de

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-6381-6742

Funding

Max-Planck-Gesellschaft (Individual research support programs)

Tobias Boothe

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.