A tunable refractive index matching medium for live imaging cells, tissues and model organisms

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Cite this article as: eLife 2017;6:e27240 doi: 10.7554/eLife.27240

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Abstract

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.

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Author details

Tobias Boothe

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Lennart Hilbert

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Michael Heide

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Lea Berninger

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Wieland B Huttner

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-4143-7201
Vasily Zaburdaev

Max Planck Institute for the Physics of Complex Systems, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Nadine L Vastenhouw

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-8782-9775
Eugene W Myers

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
David N Drechsel

Research Institute of Molecular Pathology, Dresden, Germany

Competing interests
The authors declare that no competing interests exist.
Jochen C Rink

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

For correspondence
rink@mpi-cbg.de

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-6381-6742

Funding

Max-Planck-Gesellschaft (Individual research support programs)

Tobias Boothe

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

Clare M Waterman, National Institutes of Health, United States

Publication history

Received: March 28, 2017
Accepted: July 13, 2017
Accepted Manuscript published: July 14, 2017 (version 1)
Version of Record published: September 4, 2017 (version 2)
Version of Record updated: September 14, 2017 (version 3)

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.