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A tunable refractive index matching medium for live imaging cells, tissues and model organisms

  1. Tobias Boothe
  2. Lennart Hilbert
  3. Michael Heide
  4. Lea Berninger
  5. Wieland B Huttner
  6. Vasily Zaburdaev
  7. Nadine L Vastenhouw
  8. Eugene W Myers
  9. David N Drechsel
  10. Jochen C Rink Is a corresponding author
  1. Max Planck Institute of Molecular Cell Biology and Genetics, Germany
  2. Max Planck Institute for the Physics of Complex Systems, Germany
  3. Research Institute of Molecular Pathology, Germany
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Cite as: eLife 2017;6:e27240 doi: 10.7554/eLife.27240

Abstract

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.

Article and author information

Author details

  1. Tobias Boothe

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  2. Lennart Hilbert

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Michael Heide

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  4. Lea Berninger

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  5. Wieland B Huttner

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0003-4143-7201
  6. Vasily Zaburdaev

    1. Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  7. Nadine L Vastenhouw

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0001-8782-9775
  8. Eugene W Myers

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  9. David N Drechsel

    1. Research Institute of Molecular Pathology, Dresden, Germany
    Competing interests
    The authors declare that no competing interests exist.
  10. Jochen C Rink

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    For correspondence
    1. rink@mpi-cbg.de
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0001-6381-6742

Funding

Max-Planck-Gesellschaft (Individual research support programs)

  • Tobias Boothe

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Clare M Waterman, Reviewing Editor, National Institutes of Health, United States

Publication history

  1. Received: March 28, 2017
  2. Accepted: July 13, 2017
  3. Accepted Manuscript published: July 14, 2017 (version 1)

Copyright

© 2017, Boothe et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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