In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.
- Tobias Boothe
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Clare M Waterman, Reviewing Editor, National Institutes of Health, United States
- Received: March 28, 2017
- Accepted: July 13, 2017
- Accepted Manuscript published: July 14, 2017 (version 1)
© 2017, Boothe et al.
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