In Vitro Evolution: Rethinking the tools of the RNA world

An artificially evolved ribozyme can catalyse the synthesis of RNA by using trinucleotide triphosphates as building blocks.
1 figure

Figures

Comparing monomer and triplet polymerisation in RNA-templated primer extension.

(A) An RNA polymerase ribozyme (RPR; black circle) adds a mononucleotide triphosphate (PPPN) building block to the primer (brown line) in a primer/template duplex (left). This process can be repeated (right). (B) The triplet polymerase ribozyme developed by Attwater et al. (blue circle) adds trinucleotide triphosphate (PPPNNN) building blocks and has several advantages: for example, it does not always require a primer and can copy RNA sequences in both directions. (C) The chemical structure of a trinucleotide triphosphates building block, showing the three nucleotide bases.

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  1. Antony Crisp
  2. Thomas Carell
(2018)
In Vitro Evolution: Rethinking the tools of the RNA world
eLife 7:e38297.
https://doi.org/10.7554/eLife.38297