1. Microbiology and Infectious Disease
  2. Physics of Living Systems
Download icon

Mechanics and dynamics of translocating MreB filaments on curved membranes

  1. Felix Wong
  2. Ethan C Garner
  3. Ariel Amir  Is a corresponding author
  1. Harvard University, United States
Research Advance
  • Cited 1
  • Views 1,565
  • Annotations
Cite this article as: eLife 2019;8:e40472 doi: 10.7554/eLife.40472

Abstract

MreB is an actin homolog that is essential for coordinating the cell wall synthesis required for the rod shape of many bacteria. Previously we have shown that filaments of MreB bind to the curved membranes of bacteria and translocate in directions determined by principal membrane curvatures to create and reinforce the rod shape (Hussain et al., 2018). Here, in order to understand how MreB filament dynamics affects their cellular distribution, we model how MreB filaments bind and translocate on membranes with different geometries. We find that it is both energetically favorable and robust for filaments to bind and orient along directions of largest membrane curvature. Furthermore, significant localization to different membrane regions results from processive MreB motion in various geometries. These results demonstrate that the in vivo localization of MreB observed in many different experiments, including those examining negative Gaussian curvature, can arise from translocation dynamics alone.

Article and author information

Author details

  1. Felix Wong

    School of Engineering and Applied Sciences, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2309-8835
  2. Ethan C Garner

    Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0141-3555
  3. Ariel Amir

    School of Engineering and Applied Sciences, Harvard University, Cambridge, United States
    For correspondence
    arielamir@seas.harvard.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2611-0139

Funding

National Science Foundation (DGE1144152)

  • Felix Wong

National Institutes of Health (DP2AI117923-01)

  • Ethan C Garner

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Tâm Mignot, Aix Marseille University-CNRS UMR7283, France

Publication history

  1. Received: August 2, 2018
  2. Accepted: February 15, 2019
  3. Accepted Manuscript published: February 18, 2019 (version 1)
  4. Version of Record published: May 7, 2019 (version 2)

Copyright

© 2019, Wong et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,565
    Page views
  • 345
    Downloads
  • 1
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease
    Johann Mignolet et al.
    Research Article
    1. Microbiology and Infectious Disease
    Ting Pan et al.
    Research Article Updated