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PARIS, an optogenetic method for functionally mapping gap junctions

  1. Ling Wu
  2. Ao Dong
  3. Liting Dong
  4. Shi-Qiang Wang
  5. Yulong Li  Is a corresponding author
  1. Peking University School of Life Sciences, China
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Cite this article as: eLife 2019;8:e43366 doi: 10.7554/eLife.43366

Abstract

Cell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools with both cell-type specificity and high spatiotemporal resolution. Here we developed PARIS (pairing actuators and receivers to optically isolate gap junctions), a new fully genetically encoded tool for measuring the cell-specific gap junctional coupling (GJC). PARIS successfully enabled monitoring of GJC in several cultured cell lines under physiologically relevant conditions and in distinct genetically defined neurons in Drosophila brain, with ~10-sec temporal resolution and sub-cellular spatial resolution. These results demonstrate that PARIS is a robust, highly sensitive tool for mapping functional gap junctions and study their regulation in both health and disease.

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Author details

  1. Ling Wu

    State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3921-5626
  2. Ao Dong

    State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2821-9528
  3. Liting Dong

    Peking-Tsinghua Center for Life Sciences, Peking University School of Life Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8396-374X
  4. Shi-Qiang Wang

    State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Yulong Li

    State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China
    For correspondence
    yulongli@pku.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9166-9919

Funding

National Natural Science Foundation of China (Projects 31371442)

  • Yulong Li

National Natural Science Foundation of China (Projects 31671118)

  • Yulong Li

National Natural Science Foundation of China (Projects 31630035)

  • Shi-Qiang Wang

Ministry of Science and Technology of the People's Republic of China (Grant 2015CB856402)

  • Yulong Li

Ministry of Science and Technology of the People's Republic of China (Grant 2016YFA0500401)

  • Shi-Qiang Wang

Beijing Brain Initiation (Z181100001518004)

  • Yulong Li

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Piali Sengupta, Brandeis University, United States

Publication history

  1. Received: November 3, 2018
  2. Accepted: January 12, 2019
  3. Accepted Manuscript published: January 14, 2019 (version 1)

Copyright

© 2019, Wu et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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