1. Biochemistry and Chemical Biology
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Intracellular Organization: Getting ready for DNA duplication

  1. Nina Y Yao
  2. Michael E O'Donnell  Is a corresponding author
  1. The Rockefeller University, United States
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Cite this article as: eLife 2019;8:e51291 doi: 10.7554/eLife.51291
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The molecules involved in the initiator biomolecular condensate during the G1 and S phases of the cell cycle.

(A) Parker et al. studied how three proteins – ORC (green), Cdt1 (purple) and Cdc6 (blue) – work together to help two Mcm2-7 hexamers (yellow) form a pre-replicative complex with DNA (red). (B) During the G1 phase of the cell cycle intrinsically disordered regions (IDR) in the origin of replication complex (ORC), Cdc6 and Cdt1 bind a DNA molecule to form a biomolecular condensate. (C) The biomolecular condensate forms a scaffold that the Mcm2-7 hexamer can enter, and the pre-replicative complex is formed. (D) During the S phase of the cell cycle, the IDRs of the scaffold proteins are phosphorylated (represented by the letter P), so they can no longer form condensates. Mcm2-7 hexamers loaded on the DNA can then interact with other proteins (orange and yellow) to form helicases that unwind the DNA during replication.

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