1. Developmental Biology
  2. Plant Biology

Direct ETTIN-auxin interaction controls chromatin states in gynoecium development

  1. André Kuhn
  2. Sigurd Ramans Harborough
  3. Heather M McLaughlin
  4. Bhavani Natarajan
  5. Inge Verstraeten
  6. Jiří Friml
  7. Stefan Kepinski
  8. Lars Østergaard  Is a corresponding author
  1. Department of Crop Genetics, John Innes Centre, Norwich Research Park, United Kingdom
  2. Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, United Kingdom
  3. Institute of Science and Technology, Austria
https://doi.org/10.7554/eLife.51787
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Cite this article
  1. André Kuhn
  2. Sigurd Ramans Harborough
  3. Heather M McLaughlin
  4. Bhavani Natarajan
  5. Inge Verstraeten
  6. Jiří Friml
  7. Stefan Kepinski
  8. Lars Østergaard
(2020)
Direct ETTIN-auxin interaction controls chromatin states in gynoecium development
eLife 9:e51787.
https://doi.org/10.7554/eLife.51787
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Abstract

Hormonal signalling in animals often involves direct transcription factor-hormone interactions that modulate gene expression. In contrast, plant hormone signalling is most commonly based on de-repression via the degradation of transcriptional repressors. Recently, we uncovered a non-canonical signalling mechanism for the plant hormone auxin whereby auxin directly affects the activity of the atypical auxin response factor (ARF), ETTIN towards target genes without the requirement for protein degradation. Here we show that ETTIN directly binds auxin, leading to dissociation from co-repressor proteins of the TOPLESS/TOPLESS-RELATED family followed by histone acetylation and induction of gene expression. This mechanism is reminiscent of animal hormone signalling as it affects the activity towards regulation of target genes and provides the first example of a DNA-bound hormone receptor in plants. Whilst auxin affects canonical ARFs indirectly by facilitating degradation of Aux/IAA repressors, direct ETTIN-auxin interactions allow switching between repressive and de-repressive chromatin states in an instantly-reversible manner.

Introduction

Developmental programmes within multicellular organisms originate from a single cell (i.e. a fertilised oocyte) that proliferates into numerous cells ultimately differentiating to make up specialised tissues and organs. Tight temporal and spatial regulation of the genes involved in these processes is essential for proper development of the organism. Changes in gene expression are often controlled by mobile signals that translate positional information into cell type-specific transcriptional outputs (Hironaka and Morishita, 2012). In plants, this coordination can be facilitated by phytohormones such as auxin, which controls processes throughout plant development (Vanneste and Friml, 2009). In canonical auxin signalling, auxin-responsive genes are repressed when auxin levels are low by Aux/IAA transcriptional repressors that interact with DNA-bound Auxin Response Factors (ARFs). As auxin levels increase, the auxin molecule binds to members of the TIR1/AFB family of auxin co-receptors (Kepinski and Leyser, 2005; Dharmasiri et al., 2005). The main role of this auxin perception mechanism, besides non-transcriptionally inhibiting growth (Fendrych et al., 2018; Gallei et al., 2019), is transcriptional reprogramming. This is realised by interaction with Aux/IAA repressors, leading to their ubiquitination and subsequent degradation by the 26S proteasome, ultimately relieving the repression of ARF-targeted loci (Kelley and Estelle, 2012; Leyser, 2018; Weijers and Wagner, 2016).

We recently identified an alternative auxin-signalling mechanism whereby auxin directly affects the activity of a transcription factor (TF) complex towards its downstream targets (Simonini et al., 2016; Simonini et al., 2017). This mechanism mediates precise polarity switches during organ initiation and patterning and includes the ARF, ETTIN (ETT/ARF3) as a pivotal component. However, ETT is an unusual ARF lacking the Aux/IAA-interacting Phox/Bem1 (PB1) domain (Simonini et al., 2016; Sessions et al., 1997) and is therefore unlikely to mediate auxin signalling via the canonical pathway. Here, we demonstrate that ETT binds auxin directly and that this interaction determines expression of ETT target genes. In conditions of low auxin levels, ETT interacts with co-repressors of the TOPLESS/TOPLESS-RELATED (TPL/TPR) family to keep chromatin at ETT target loci in a repressed state through HDA19-mediated histone deacetylation. Under high auxin conditions, ETT binds auxin leading to dissociation of TPL/TPR-HDA19 and de-repression of ETT targets.

Results

ETT can interact with a diverse set of TFs and these interactions are sensitive to the naturally occurring auxin, indole 3-acetic acid (IAA) (Simonini et al., 2016). To test if this effect is mediated through a direct interaction between ETT and auxin, we took advantage of the drug affinity-responsive target stability (DARTS) assay (Lomenick et al., 2011) and followed the effect of IAA on proteolytic degradation of a FLAG-fused ETT segment conditionally expressed in Arabidopsis (Figure 1a). The concentrations of IAA applied (0.1 µM, 1 µM, 10 µM) were selected to saturate the protein with ligand and to ensure maximal protection from proteolysis. As a negative control, benzoic acid (BA) was applied at the same dose as IAA (10 µM and 100 µM) because of its similar size and pKa as IAA. Treatments with IAA but not BA protected ETT-FLAG from pronase-induced degradation consistent with direct, specific interaction between IAA and ETT in planta.

Figure 1 with 2 supplements see all
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ETT directly binds auxin (IAA).

(a) DARTS assay suggests that ETT binds IAA. Seedlings from an inducible two-component ETT-FLAG line were used for the protein isolation. Samples were treated with DMSO (mock), IAA and BA and digested by different concentrations of Pronase. Samples were further analysed by western blot with an anti-FLAG antibody. (b) HSQC-NMR performed with ES388-594 protein either alone (black), with indole-3-acetic acid (IAA, green) or benzoic acid (BA, pink). ES388-594: ligand, 1:10, 50 µM:500 µM. (c) Zoom-in of the indicated rectangular region in a. (d) Zoom-in of the specific shifts (labelled I-V) in the indicated dotted rectangles in b and c. Changes in chemical shifts are indicated by arrows from control to IAA treatment. (e–g) ITC spectre showing heat exchange between ES388-594 protein and IAA (e), but not in controls (f, g). See Figure 1—figure supplement 1 for parameters used in the HSQC-NMR experiment.

Figure 1—source data 1

Parameters for HSQC NMR.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig1-data1-v2.xlsx

The region responsible for IAA-sensitivity is situated within the C-terminal part of ETT, known as the ETT-Specific (ES) domain. A protein fragment containing 207 amino acids of the ES domain, ES388-594, sufficient for mediating IAA-sensitivity in ETT-protein interactions, was produced recombinantly and shown to be intrinsically disordered (Simonini et al., 2018). The sensitivity of ETT-TF interactions to IAA suggests a direct effect of the IAA molecule on the ETT protein. Therefore, to test whether ETT binds IAA, we carried out heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) experiments using 15N-labelled ES388-594 protein. The HSQC spectrum, recorded at 5 °C, shows a prominent signal-dense region consistent with the ES domain being largely intrinsically disordered. Interestingly, the spectrum also shows dispersed peaks flanking the signal-dense region indicating that there is nevertheless some propensity to form secondary structure, particularly with a helical character (Figure 1b). In addition to this overview of ETT structure, the HSQC NMR probes chemical shifts of protein amide-NH bonds in response to the presence of ligand (Meyer and Peters, 2003). We found that a number of residues shifted their position in the spectrum in response to the addition of IAA, whereas addition of BA had no effect (Figure 1b–d). These shifts show that certain residues are experiencing a changed chemical environment as a consequence of IAA-binding and this may include the conformational change of a structural motif within the ETT protein. The HSQC experiment therefore demonstrates that ETT binds IAA directly. This experiment has not allowed us to assign signals to specific amino acids and hence there is some uncertainty associated with tracking the chemical shifts of some residues. However, a particularly large change is observed for the tryptophan NH cross peak when IAA is added to the ETT fragment (~10 ppm, rectangle I in Figure 1b,d). Since there is only one tryptophan in the ETT fragment used here (W505), this shift can be assigned to this residue and suggests that W505 has a prominent role in the interaction with IAA.

We also used the recombinant ETT fragment in an Isothermal Titration Calorimetry (ITC) assay, which characterises binding of ligands to proteins by determining thermodynamic parameters of the interaction as heat exchange. This experiment revealed interaction between ETT and IAA, while control experiments titrating IAA into buffer without protein and titrating buffer without IAA into the ETT fragment showed no heat exchange (Figure 1e–g). We were unable to obtain a Kd value for the interaction, which may be due to the interaction being weak as well as potential protein aggregation. This could reflect that the ETT-auxin interaction is stabilised by interacting protein partners in planta that are not present in this in vitro experiment.

Together, these three independent biochemical methods demonstrate that ETT binds IAA directly thus revealing a key molecular aspect of the non-canonical auxin-signalling pathway.

Previously, PINOID (PID) (Benjamins et al., 2001) and HECATE1 (HEC1) (Gremski et al., 2007) were identified as ETT target genes (Simonini et al., 2016; Simonini et al., 2017) and both genes are upregulated in gynoecium tissue from the ett-3 mutant compared to wild type (Figure 2—figure supplement 1). We also observed that expression of both genes is induced by IAA, but did not observe any additional induction beyond the constitutive upregulation in the ett-3 mutant background (Figure 2—figure supplement 1). This ETT-dependent regulation does not require a functional TIR1/AFB machinery, since IAA-induction of PID and HEC1 is still observed in tir1/afb mutant combinations, whereas the known TIR1/AFB-mediated auxin induction of the IAA19 gene is completely abolished in these mutants (Figure 2a–c).

Figure 2 with 1 supplement see all
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ETT regulates target gene expression independently of TIR1/AFB auxin receptors.

Expression of the canonical auxin responsive IAA19 gene (a) and the ETT-target genes HEC1 (b) and PID (c) in control-treated (dark grey) or 100 µM IAA-treated (light grey) gynoecia assayed using qRT-PCR. (a) IAA19 expression is up-regulated in response to auxin in wild-type gynoecia (Col-0) but not in tir1/afb double and triple mutants. The ETT-target genes HEC1 and PID are up-regulated in response to auxin in both wild-type and auxin receptor mutants (b c). This suggests a TIR1/AFB independent regulation of these genes. (d) Expression of IAA19, HEC1 and PID in response to treatment with 100 µM IAA and 100 µM cvxIAA in wild-type (Col-0) and pTIR1:ccvTIR1 gynoecia in the tir1 afb2 double mutant (ccvTIR1). The data confirm TIR1/AFB independent regulation of HEC1 and PID in the gynoecium. ***p<0.0001; Shown are mean ± standard deviation of three biological replicates. See Figure 2—source data 2 for statistical analyses.

Figure 2—source data 1

Oligonucleotides used in this study.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig2-data1-v2.xlsx
Figure 2—source data 2

Output of statistical tests.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig2-data2-v2.xlsx

To further assess the TIR1/AFB independence of the ETT-mediated auxin signalling pathway, we exploited a recently-developed synthetic auxin-TIR1 pair (Uchida et al., 2018). In this system, the auxin-binding pocket of TIR1 has been engineered (ccvTIR1) to accommodate an IAA derivative bearing a bulky side chain (cvxIAA). By expressing the ccvTIR1 in a tir1 afb2 mutant background, the canonical pathway will only respond to the addition of cvxIAA and not IAA (Uchida et al., 2018). We performed an expression analysis on ccvTIR1 gynoecia treated ±cvxIAA and ±IAA as well as control plants with the same treatments. In this experiment, IAA19 served as a control gene whose expression is regulated in a TIR1/AFB-dependent manner (Benjamins et al., 2001). Indeed, IAA19 was strongly upregulated by cvxIAA in the ccvTIR1 line, but not by IAA (Figure 2d). In contrast, PID and HEC1 expression was not significantly affected by cvxIAA, whilst still responding to IAA in the ccvTIR1 background (Figure 2d). These data demonstrate that ETT-mediated auxin signalling can occur independently of the canonical TIR1/AFB signalling pathway.

In a phylogenetic analysis of ETT protein sequences across the angiosperm phylum, we identified a number of regions that are highly conserved (Figure 3—figure supplement 1). Unsurprisingly, the DNA-binding domain characteristic to B3-type TFs such as ARF proteins was conserved across all ETT proteins. Towards the C terminus of the ES domain we identified an EAR-like motif with a particularly high level of conservation (Figure 3a, Figure 3—figure supplement 1a). Ethylene-responsive element binding factor-associated Amphiphilic Repression (EAR) motifs are also found in Aux/IAA proteins. Interactions between Aux/IAA and members of the TOPLESS and TOPLESS-RELATED (TPL/TPR) family of co-repressors occur via this motif (Szemenyei et al., 2008). TPL/TPRs mediate their repressive effect by attracting histone deacetylases (HDACs) to promote chromatin condensation (Krogan et al., 2012). It was recently shown that ETT is required to keep Histone H3 at a deacetylated state at the gene locus of the meristem identity gene SHOOT MERISTEMLESS (STM) thereby repressing STM expression (Chung et al., 2019). Since ETT functions independently of the canonical auxin pathway, it is possible that its role in chromatin remodelling occurs via direct interaction with TPL/TPRs through the EAR-like motif. To test this, we carried out Yeast 2-Hybrid (Y2H) assays in which ETT was found to interact with TPL, TPR2 and TPR4 (Figure 3b, Figure 3—figure supplement 1b). Moreover, mutating residues in the EAR-like motif abolished the interactions demonstrating its requirement for the ETT-TPL/TPR interaction (Figure 3b).

Figure 3 with 3 supplements see all
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ETT interacts with members of the TPL/TPR co-repressor family in an auxin-sensitive manner.

(a) Schematic representation of full-length ETT protein highlighting an EAR-like motif and tryptophan in position 505 (W) in the C-terminal ETT-specific domain. DBD, DNA-binding domain. (b) Y2H showing that ETT interacts with TPL, TPR2 and TPR4. These interactions depend on the identified C-terminal RLFGF motif and are auxin-sensitive. DBD, DNA-binding domain. (c) Co-IP revealing that ETT interacts with TPL in an auxin-sensitive manner with increasing IAA concentrations weakening the interaction. (d) Co-IP showing that mutating the tryptophan in position 505 of ETT (ETTW505A) leads to loss of auxin sensitivity in the interaction between ETT and TPL.

Figure 3—source data 1

Output of statistical tests.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig3-data1-v2.xlsx

Given that several ETT-protein interactions are affected by IAA and that part of the ETT transcriptome changes in response to IAA (Simonini et al., 2016; Simonini et al., 2017), we tested the IAA sensitivity of ETT-TPL/TPR interactions. In both Y2H and in co-immunoprecipitation (Co-IP) experiments, we observed that the interactions were reduced with increasing IAA concentrations (Figure 3b,c and Figure 3—figure supplement 2). Moreover, as described previously for other ETT-protein interactions, the sensitivity was specific to IAA as other auxinic compounds tested did not show this effect (Figure 3—figure supplement 2). Henceforth, ‘auxin’ will refer to IAA unless stated otherwise. These data suggest that in conditions with low auxin levels, ETT can interact with TPL/TPR proteins to repress the expression of target genes. An increase in cellular auxin causes ETT to bind auxin thereby undergoing a conformational change that abolishes interaction with TPL/TPR co-repressors.

The HSQC-NMR experiment described above indicate that the tryptophan in position 505 (W505) affects the direct interaction between the ETT protein and auxin. To test the role of W505 in the auxin-sensitive interaction with TPL/TPRs, we constructed a mutated version of ETT (W505A) and assessed the effect in protein interaction assays. Both Y2H and Co-IP experiments revealed that W505 is required for the ETT-TPL/TPR interaction to be sensitive to auxin (Figure 3c,d). The key importance of W505 is furthermore supported by a phylogenetic analysis of ETT protein sequences revealing that this residue is highly conserved in ETT proteins across angiosperms (Figure 3—figure supplement 1a).

TPL was originally identified as a key factor involved in setting up the apical-basal growth axis during embryo development (Long et al., 2006; Smith and Long, 2010). Large-scale interaction studies suggest that the five Arabidopsis TPL/TPRs have roles throughout plant development (Krogan et al., 2012; Causier et al., 2012). Whilst ETT has been implicated in a wide array of developmental processes (Garcia et al., 2006; Marin et al., 2010; Kelley et al., 2012; Pekker et al., 2005), the most dramatic phenotypes of ett loss-of-function mutants are observed during gynoecium development (Sessions et al., 1997; Sessions and Zambryski, 1995; Nemhauser et al., 2000). In accordance with this, ETT is highly expressed in the gynoecium (Figure 4a; Simonini et al., 2016). We produced reporter lines of TPL, TPR2 and TPR4 promoters fused to the GUS gene to test if they overlap with ETT expression in the gynoecium. Both pTPL:GUS and pTPR2:GUS exhibited strong expression in the apical part of the gynoecium where ETT is also expressed, while no pTPR4:GUS expression was observed (Figure 4a–d). Single loss-of-function mutants in TPL and TPR2 do not show any abnormal phenotypes during gynoecium development. However, the tpl tpr2 double mutant has defects in the development of the apical gynoecium similar to ett mutants (Figure 4e–g) demonstrating that TPL and TPR2 function redundantly in gynoecium development. Together with the protein interaction data and the overlapping expression patterns, these results suggest that ETT and TPL/TPR2 cooperate to regulate gynoecium development.

Figure 4 with 1 supplement see all
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ETT, TPL/TPR2 and HDA19 co-operatively regulate gene expression to facilitate gynoecium development.

(a–d) Promoter GUS expression analysis of pETT:GUS (a), pTPL:GUS (b), pTPR2:GUS (c) and pTPR4:GUS (d) revealed that ETT, TPL and TPR2 but not TPR4 are co-expressed in the Arabidopsis style. Scale bar = 300 μm. (e–h) Gynoecium phenotypes of wild-type (e), ett-3 (f) tpl tpr2ge (g) and hda19-4 (h). Scale bar = 100 μm. (i j,) HEC1 (i) and PID (j) are constitutively mis-regulated in ett-3, tpl tpr2ge and hda19-4 gynoecia. This misregulation is unaffected by treatment with 100 µM IAA. ***p-values<0.0001; Shown are mean ± standard deviation of three biological replicates. Differences between untreated and IAA-treated mutants are not significant. See Figure 4—source data 1 for statistical analyses.

Figure 4—source data 1

Output of statistical tests.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig4-data1-v2.xlsx

TPL was shown previously to recruit histone deacetylase, HDA19, during early Arabidopsis flower development to keep chromatin in a repressed state (Krogan et al., 2012). Moreover, HDA19 was also recently shown to participate in the repression of STM (Chung et al., 2019). In that study, ETT was shown to recruit HDA19 to the STM promoter, although not via direct protein interaction. Here, our analysis of gynoecia from the hda19-4 mutant demonstrate that HDA19 is also required for gynoecium development as the hda19-4 mutant has strong style defects (Figure 4h). In agreement with this, the HDA19 gene was highly expressed in gynoecium tissue, whereas another member of the HDA gene family, HDA6, was not (Figure 4—figure supplement 1). Moreover, HDA19 recruitment likely involves ETT, since expression of the ETT target genes, PID and HEC1, is increased in the tpl tpr2 and hda19-4 mutants compared to wild type. Similar to the ett mutant, auxin treatments failed to further induce expression in these mutants (Figure 4i,j). These observations suggest that ETT, TPL/TPR2 and HDA19 function in conjunction to control gene expression during gynoecium development.

To test the direct interaction of ETT, TPL and HDA19 on chromatin, we performed Chromatin-Immunoprecipitation (ChIP) using reporter lines expressing GFP fusion protein. Although only ETT is expected to bind DNA, ChIP followed by qPCR revealed that all three proteins associate with DNA elements in the same regions of the promoters of PID and HEC1 (Figure 5a–c). Moreover, while ETT association with these promoter regions was largely unaffected in the presence of IAA, TPL and HDA19 interactions are reduced upon auxin treatment (Figure 5a–c). This supports a model in which ETT recruits TPL/TPR2 and HDA19 to ETT target loci to keep chromatin in a condensed state through histone deacetylation. When auxin levels increase, the ETT-TPL/TPR2 interaction is broken and TPL/TPR2 and HDA19 dissociate from the loci, presumably preventing HDA19 from deacetylating histones. To test this, we assayed for H3K27 acetylation, which is a substrate for HDA19. H3K27 acetylation increased in the absence of ETT and upon treatment with auxin. This occurred in the same regions of the PID and HEC1 promoters where the proteins were found to associate (Figure 5d,e). In agreement with ETT mediating the association of TPL/TPR and HDA19 with these regions, there was no further increase of acetylation in the ett-3 mutant upon treatment with auxin (Figure 5d,e).

Figure 5
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ETT, TPL and HDA19 co-operatively regulate HEC1 and PID by modulating chromatin acetylation.

(a–c) Chromatin immunoprecipitation (ChIP) shows ETT (a), TPL (b) and HDA19 (c) binding to conserved regions of HEC1 and PID loci in the absence of IAA (dark grey columns). In the presence of IAA (light grey columns), ETT association is unchanged, while both TPL and HDA19 interactions are reduced. A WUS gene fragment served as negative control (NC). (d e,) H3K27ac accumulation (from ChIP analysis) along the HEC1 (d) and PID (e) loci in wild-type (Col-0) and ett-3 plants ± treatment with 100 µM IAA. Numbers on the x axes are distances to the Transcription Start Site (TSS). The schematic of the loci is shown below each panel. Dashed boxes represent ETT binding regions. (f) Qualitative schematic illustration of alternative TIR1/AFB independent auxin signaling. Under low auxin conditions an ETT-TPL-HDA19 complex binds to ETT-target genes keeping their chromatin environments repressed, through de-acetylation. High nuclear auxin concentrations abolish the ETT-TPL-HDA19 complex through direct ETT-auxin interaction. This leads to an accumulation of histone acetylation and up-regulation of ETT-target genes. Values in a–e) are means ± standard deviation of three biological replicates. See Figure 5—source data 1 for statistical analyses.

Figure 5—source data 1

Output of statistical tests.

https://cdn.elifesciences.org/articles/51787/elife-51787-fig5-data1-v2.xlsx

Discussion

The data presented in this paper provide molecular insight into how auxin levels are translated into changes in gene expression of ETT target genes. Our data lead to a model in which low levels of auxin maintain ETT associations with TPL/TPR2 to repress gene expression via H3K27 deacetylation. As auxin levels increase, TPL/TPR2 (and hence HDA19) disassociate from ETT, promoting H3K27 acetylation (Figure 5f). This model molecularly underpins the published association between auxin dynamics and PID expression at the gynoecium apex where PID is repressed at early stages of development to allow symmetry transition, but subsequently de-repressed as auxin levels rise to facilitate polar auxin transport (Simonini et al., 2016; Moubayidin and Ostergaard, 2014). Whilst this model explains the data on ETT-mediated auxin signalling during gynoecium development, it is possible that the effect of auxin on ETT varies depending on the developmental context. Moreover, the concentrations of auxin required to mediate its effect on ETT is likely to differ between diverse ETT-containing complexes.

The direct binding of auxin allows ETT to switch the chromatin locally between repressive and de-repressive states. The recent report of ETT negatively regulating STM expression (Chung et al., 2019) provides molecular evidence that ETT can function as a repressor. Whether ETT also has a role in recruiting activating components is yet to be clarified. Indeed, different modes of actions are possible that could depend on other interacting partners or auxin levels and even be target-gene specific. Nevertheless, the effect of auxin on ETT-mediated regulation of the genes studied here is instantly reversible, making it possible to switch between states, immediately responding to changes in auxin levels. This feature, which is reminiscent of animal hormonal signalling pathways such as the Thyroid Hormone and Wnt/ß-catenin pathways (Tsai and O'Malley, 1994; Gammons and Bienz, 2018), may be particularly important in controlling changes in tissue polarity during plant organogenesis as observed in the Arabidopsis gynoecium (Moubayidin and Ostergaard, 2014).

The identification of a direct auxin-ETT interaction to control gene expression adds an additional layer of complexity to auxin action, which contributes towards explaining how auxin imparts its effect on highly diverse processes throughout plant development. In a broader context, this work also opens for the exciting possibility that direct transcription factor-ligand interactions is a general feature in the control of gene expression in plants as found in animals.

Materials and methods

Key resources table
Reagent type
(species) or resource		DesignationSource or referenceIdentifiersAdditional
information
Gene (Arabidopsis thaliana)ETTIN (ETT)The Arabidopsis Information ResourceAT2G33860
Gene (Arabidopsis thaliana)TOPLESS
(TPL)		The Arabidopsis Information ResourceAT1G15750
Gene (Arabidopsis thaliana)TOPLESS- RELATED 1 (TPR1)The Arabidopsis Information ResourceAT1G80490
Gene
(Arabidopsis thaliana)		TOPLESS-RELATED 2 (TPR2)The Arabidopsis Information ResourceAT3G16830
Gene (Arabidopsis thaliana)TOPLESS-RELATED 3 (TPR3)The Arabidopsis Information ResourceAT5G27030
Gene (Arabidopsis thaliana)TOPLESS-RELATED 4 (TPR4)The Arabidopsis Information ResourceAT3G15880
Gene (Arabidopsis thaliana)HISTONE DEACETYLASE 6
(HDA6)		The Arabidopsis Information ResourceAT5G63110
Gene (Arabidopsis thaliana)HISTONE DEACETYLASE 19
(HDA19)		The Arabidopsis Information ResourceAT4G38130
Gene (Arabidopsis thaliana)HECATE 1
(HEC1)		The Arabidopsis Information ResourceAT5G67060
Gene (Arabidopsis thaliana)PINOID (PID)The Arabidopsis Information ResourceAT2G34650
Genetic reagent Arabidopsis thalianaCol-0widely distributed
Genetic reagent Arabidopsis thalianahda19-4Kim et al., 2008SALK_13944
Genetic reagent Arabidopsis thalianatir1-1 afb2-3 afb3-4Parry et al., 2009
Genetic reagent Arabidopsis thalianatplEuropean Arabidopsis Stock CentreSALK_034518C
Genetic reagent Arabidopsis thalianaett-3Simonini et al., 2017; Sessions et al., 1997AT2G33860
Genetic reagent Arabidopsis thalianapETT:GUSNg et al., 2009AT2G33860
Genetic reagent Arabidopsis thalianapETT:ETT-GFPSimonini et al., 2016AT2G33860
Genetic reagent Arabidopsis thaliana pTPL:TPL-GFPPi et al., 2015AT1G15750
Genetic reagent Arabidopsis thalianap35S:HDA19-GFPPi et al., 2015AT4G38130
Genetic reagent Arabidopsis thalianapTIR1:ccvTIR1Szemenyei et al., 2008
Genetic reagent Arabidopsis thaliana tpl tpr2gethis paperAT1G15750, AT3G16830Further details in the Materials and methods section
Genetic reagent Arabidopsis thalianapTPL:GUSthis paperAT1G15750Further details in the Materials and methods section
Genetic reagent Arabidopsis thalianapTPR2:GUSthis paperAT3G16830Further details in the Materials and methods section
Genetic reagent Arabidopsis thalianapTPR4:GUSthis paperAT3G15880Further details in the Materials and methods section
Genetic reagent Arabidopsis thalianap35S:CDGVG p6xGAL4UAS:ETT-FLAGthis paperFurther details in the Materials and methods section
AntibodyMouse anti-FLAGAbcamab49763monoclonal, conjugated with HRP
AntibodyMouse anti-HAAbcamab173826monoclonal, conjugated with HRP
AntibodyMouse anti-GFPRoche11814460001monoclonal
AntibodyRabbit anti-H3K27acAbcamab4729polyclonal
AntibodyRabbit anti-H3Abcamab1791polyclonal
Recombinant DNA reagentpGWB14; p35s:HA-TPLEspinosa-Ruiz et al., 2017
Recombinant DNA reagentpICH47732; p35s:ETT-3xFLAGthis paperBackbone Addgene
#48000
Recombinant DNA reagentpICH47732; p35s:
ETTW505A-3xFLAG		this paperBackbone Addgene
#48000
Recombinant DNA reagentpGADT7; ETTthis paperFurther details in the Materials and methods section
Recombinant DNA reagentpGADT7; ETTW505Athis paperFurther details in the Materials and methods section
Recombinant DNA reagentpGADT7; ETTL552S; F553Sthis paperFurther details in the Materials and methods section
Recombinant DNA reagentpGBKT7; TPLCausier et al., 2012
Recombinant DNA reagentpGBKT7; TPR1Causier et al., 2012
Recombinant DNA reagentpGBKT7; TPR2Causier et al., 2012
Recombinant DNA reagentpGBKT7; TPR3Causier et al., 2012
Recombinant DNA reagentpGBKT7; TPR4Causier et al., 2012
Recombinant DNA reagentpESPRIT; ES388-594Simonini et al., 2018

Plant materials and treatments

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Plants were grown in soil at 22°C in long day conditions (16 hrs day/8 hr dark). All mutations were in the Col-0 background. Mutant alleles described before include ett-3 (Sessions et al., 1997; Simonini et al., 2016), hda19-4 (SALK_139443) (Kim et al., 2008), pETT:GUS (Ng et al., 2009), pETT:ETT-GFP in ett-3 (Simonini et al., 2016), pTPL:TPL:GFP (Pi et al., 2015), p35S:HDA19:GFP (Pi et al., 2015), pTIR1:ccvTIR1 in tir1-1 afb2-3 (Szemenyei et al., 2008) and tir1-1 afb2-3 afb3-4(Parry et al., 2009). The tpl mutant (SALK_034518C) was obtained from the European Arabidopsis Stock Centre.

For both expression and ChIP analysis, auxin treatments were applied by spraying bolting Col-0 and ett-3 inflorescences with a solution containing 100 µM IAA (Sigma) or cvxIAA and 0.015% Silwet L-77 (De Sangosse Ltd.). Treated samples were returned to the growth room and incubated for two hours.

Expression analysis

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Quantitative Real time PCR (qRT-PCR) was used for expression analysis. RNA was extracted from floral buds using the RNeasy mini kit (Qiagen). Using the SuperScript IV First-Strand Synthesis kit (ThermoFisher), cDNA was synthesised from 1 µg of total RNA. Subsequently, qRT-PCR was carried out using SYBR Green JumpStart Taq ReadyMix (Sigma) using the appropriate primers (Figure 2—source data 1). Relative expression values were determined using the 2-ΔΔCt method (Livak and Schmittgen, 2001). Data were normalised to POLYUBIQUITIN 10 (UBQ10/AT4G05320) expression.

ETT protein analysis by alignment

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Published ETT sequences of 22 Angiosperm species were retrieved from Phytozome version 12 (Goodstein et al., 2012). Nucleotide sequences were translated and aligned using MUSCLE in Geneious version 6.1.8 (Kearse et al., 2012). The EAR-like motif was extracted as a sequence logo (Figure 3a; Figure 3—figure supplement 1).

Generation of the tpl tpr2 CRISPR mutant

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The tpl tpr2ge mutant was generated using CRISPR/Cas9 technology by a method previously described (Castel et al., 2019). Briefly, for the construction of the RNA-guided genome-editing plasmid, DNA sequences encoding the gRNA adjacent to the PAM sequences were designed to target two specific sites in TPR2 (AT3G16830). DNA-oligonucleotides (Figure 2—source data 1) containing the specific gRNA sequence were synthesised and used to amplify the full gRNA from a template plasmid (AddGene #46966). Using Golden Gate cloning (Engler et al., 2014) each gRNA was then recombined in a L1 vector downstream of U6 promoter (Goodstein et al., 2012). Finally, the resulting gRNA plasmids were then recombined with a L1 construct containing pYAO:Cas9_3:E9t (Castel et al., 2019) (kindly provided by Jonathan Jones) and a L1 construct containing Fast-Red selection marker (AddGene #117499) into a L2 binary vector (AddGene #112207).

The construct was transformed into Agrobacterium tumefaciens strain GV3101 by electroporation, followed by plant transformation by floral dip into the tpl single mutant (Clough and Bent, 1998). Transgenic T1 seeds appear red under UV light and were selected under a Leica M205FA stereo microscope. T1 plants were genotyped using PCR and the TPR2 locus sequenced (Oligonucleotides in Figure 2—source data 1). Genome-edited plants were selected and the next generation grown (T2). Seeds of this generation were segregating in a 3:1 ratio for the transgene. Transgene negative plants were selected and grown on soil. To identify homozygous mutations T2 plants were genotyped. The T3 generation was again checked for the absence of the transgene.

Protein interaction

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For Yeast-two-Hybrid (Y2H) assays coding sequences were cloned into pDONR207 and recombined into the pGDAT7 and pGBKT7 (Clontech). Using the co-transformation techniques (Engler et al., 2014) these constructs were transformed into the AH109 strain (Clontech). Transformations were selected on Yeast Selection Medium (YSD) lacking Tryptophan (W) and Leucine (L) at 28°C for 3–4 days. Transformed yeast cells were serially diluted (100, 10-1, 10-2 and 10-3) and dotted on YSD medium lacking Tryptophan (W), Leucin (L), adenine (A) and Histidine (H) to test for interaction. To examine interaction strength 3-amino-1,2,4-triazole (3-AT) was supplemented to the YSD (-W-L-A-H) medium with different concentrations (0, 5, 10 mM). To determine the effect of auxinic compounds on the protein-protein interactions benzoic acid (BA), IAA, NAA and 2,4D (all Sigma) were dissolved in ethanol and added directly to the medium at the desired concentrations. Pictures were taken after 3 days of growth at 28°C.

For the β-Galactosidase assay, transgenic yeast was grown in liquid YSD (-W-L) medium supplemented with /-out 100 µM IAA or NAA, to an OD600 of 0.5. The cells were then harvested and lysed using 150 µL Buffer Z with β-mercaptoethanol (100 mM Phosphate buffer pH 7, 10 mM KCl, 1 mM Mg2SO4, β-mercaptoethanol 50 mM), 50 µL chloroform and 20 µL of 0.1% SDS. After lysis, the sample was incubated with 700 µL pre-warmed ONPG solution (1 mg/mL ONPG (o-Nitrophenyl-β-D-Galactopyranoside, Sigma) prepared in Buffer Z without β-mercaptoethanol at 37°C until a yellow colour developed in the samples without auxin treatment. After stopping all reactions (using 500 µL Na2CO3) the supernatant was collected and OD405 determined. The β-Galactosidase activity was calculated as follows: (A405*1000)/(A600*min*mL).

For co-immunoprecipitation, ETT-FLAG was generated using Golden Gate cloning (Kearse et al., 2012) by recombining a previously described L0 clone for ETT (Simonini et al., 2016) with a 35S promoter (AddGene #50266), a C-terminal 3xFLAG epitope (AddGene #50308) and a Nos-terminator (AddGene #50266) into a L1 vector (AddGene #48000). The pGWB14 TPL-HA construct was provided by Salomé Prat and has been used in previous studies (Espinosa-Ruiz et al., 2017). The epitope-tagged proteins were transiently expressed in four-week-old N. benthamiana leaves for two days. Co-immunoprecipitation was performed as described previously (Egea-Cortines et al., 1999). After harvest, 1 g of fresh leaf tissue was ground in liquid nitrogen. The powder was homogenised for 30 min in two volumes of extraction buffer (10% glycerol, 25 mM Tris-HCl pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.15% NP-40, 1 mM PMSF, 10 mM DTT, 2% Polyvinylporrolidone, 1x cOmplete Mini tablets EDTA-free Protease Inhibitor Cocktail (Roche). The homogenised samples were cleared by centrifugation at 14,000 x g for 10 min and cleared lysates were incubated for 2 hr with 20 µl anti-FLAG M2 magnetic beads (SIGMA-ALDRICH, M8823; lot: SLB2419). The beads were washed five times with IP buffer (10% glycerol, 25 mM Tris-HCl pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.15% NP-40, 1 mM PMSF, 1 mM DTT, 1x cOmplete Mini tablets EDTA-free Protease Inhibitor Cocktail (Roche)) and proteins were eluted by adding 80 µl 2x SDS loading buffer followed by an incubation at 95°C for 10 min. To examine auxin sensitivity, 4 g of fresh leaf tissue was collected, ground in liquid nitrogen and protein was extracted. The lysate was then divided according to the number of treatments. The desired concentration of IAA or NAA was added to each of the cleared lysates before the anti-FLAG M2 magnetic beads were added. IAA or NAA at the desired concentration was also supplemented to the IP buffer during the washes. The eluates were analysed by western blot using an anti-FLAG antibody (M2, Abcam, ab49763, Lot: GR3207401-3) or an anti-HA antibody (Abcam, ab173826, Lot: GR3255539-1). Both antibodies were used as 1:10000 dilutions. The antibodies were validated by the manufacturer.

Scanning electron microscopy

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Whole inflorescences of Col-0, ett-3, tpl tpr2ge and hda19-4 were fixed overnight in FAA (3.7% formaldehyde, 5% glacial acetic acid, 50% ethanol) and dehydrated through an ethanol series (70% to 100%) as described previously (Moubayidin and Ostergaard, 2014). The samples were then critical point-dried, gynoecia dissected and mounted. After gold coating, samples were examined with a Zeiss Supra 55VP Field Emission Scanning electron microscope using an acceleration voltage of 3 kV.

TPL, TPR2 and TPR4 reporter lines

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For the construction of the promoter:GUS reporter plasmids of TPL, TPR2 and TPR4, 2.5 kb of promoter sequences were isolated from genomic DNA and inserted upstream of the ß-glucoronidase gene of pCambia1301 vectors using the In-Fusion Cloning Recombinase kit (Clontech). The constructs were transformed into Agrobacterium tumefaciens strain GV3101 by electroporation, followed by plant transformation by floral dip into Col-0 (Clough and Bent, 1998).

The GUS histochemical assay was performed in at least three individual lines per construct. Inflorescences of each GUS line were pre-treated with ice cold acetone for 1 hr at −20°C and washed two times for 5 min with 100 mM sodium phosphate buffer followed by one wash with sodium phosphate buffer containing 1 mM K3Fe(CN)6 and 1 mM K4Fe(CN)6 (both Sigma) at room temperature. Subsequently, samples were vacuum infiltrated for 5 min with X-Gluc solution (100 mM sodium phosphate buffer, 10 mM EDTA, 0.5 mM K3Fe(CN)6, 3 mM K4Fe(CN)6, 0.1% Triton X100) containing 1 mg/ml of ß-glucoronidase substrate X-gluc (5-bromo-4-chloro-3-indolylglucuronide, Melford) and incubated at 37°C. pTPL:GUS samples were incubated for 20 min and pTPR2:GUS lines for 45 min to prevent overstaining. pTPR4:GUS lines were incubated for 16 hr. After staining, the samples were washed in 70% ethanol until chlorophyll was completely removed. Gynoecia were dissected and mounted in chloral hydrate (Sigma). Samples were analysed using a Leica DM6000 light microscope.

Chromatin immunoprecipitation

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Transcription factor ChIP was performed in biological triplicates using the pETT:ETT:GFP, pTPL:TPL:GFP and p35S:HDA19-GFP lines and data analysed as described previously (Chung et al., 2019). Additionally, a WUS gene fragment (WUSp4 amplicon) was used as a negative control for ETT binding (Liu et al., 2014). IP was conducted using the anti-GFP antibody (Roche, 11814460001, Lot: 19958500) and Pierce Protein G magnetic beads (ThermoFisher, 88847, Lot: SI253639) were used for IP.

Histone acetylation ChIP was carried out and data were analysed as described previously (Qüesta et al., 2016). The experiment was carried out in triplicate using 3 g auxin-treated or untreated Col-0 or ett-3 inflorescent tissue. The antibodies used for IP were anti-H3K27ac antibodies (Abcam, ab4729, Lot: GR3231937-1) and anti-H3 (Abcam, ab1791, Lot: GR310541-1). All antibodies were validated by the manufacturers.

In all ChIP experiments, DNA enrichment was quantified using quantitative PCR (qPCR) with the appropriate primers (Figure 2—source data 1). In case of H3K27ac, H3 was used as an internal control and the data represented as ratio of (H3K27ac at HEC1 or PID divided by H3 at HEC1 or PID).

Statistical analyses and replication

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In all graphs error bars represent the standard deviation of the mean for all numerical values. QRT-PCR and ChIP experiments have been carried out at least in triplicate. The data presented here show an average of three replicates. For qRT-PCR data were analysed using one-way ANOVA with post-hoc Tukey multiple comparison test. ChIPqPCR data were analysed using two-way ANOVA with post hoc Bonferroni multiple comparison test. All output of statistical tests can be found in the source data files. All statistical tests were carried out using GraphPad Prism Version 5.04 (La Jolla California USA, www.graphpad.com).

Drug Affinity Responsive Target Stability (DARTS) assay

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The DARTS assay to evaluate the stability of ETTIN-FLAG in the presence of IAA was performed as described previously (Lomenick et al., 2011; Kania et al., 2018; Tan et al., 2020) with plant material of a two-component inducible ETTIN-FLAG line. This ETT-FLAG line was generated using Golden Gate cloning (Engler et al., 2014) by recombining a previously described L0 clone for ETT (Simonini et al., 2016) with a 6xGAL4UAS promoter, a C-terminal 3xFLAG epitope (AddGene #50308) and a Nos-terminator (AddGene #50266) into an L1 vector (AddGene #48000). This inducible ETTIN-FLAG cassette was combined with a dexamethasone inducer cassette (containing CD-GVG under a 35S promoter) and a phosphinothricin-resistance cassette in an L2 binary vector (AddGene #48015). The construct was transformed into Agrobacterium tumefaciens strain GV3101 by electroporation, followed by floral dip-mediated plant transformation into Arabidopsis Col-0. For the DARTS assay, seeds were surface sterilised using chlorine gas and germinated in 25 mL liquid ½ MS medium containing 1% sucrose in 100 mL Erlenmeyer flasks under gently shaking. ETTIN-FLAG expression was induced 7 days post germination by adding 10 µM dexamethasone to the growth medium and seedlings were harvested 48 hr post induction. Total plant material was ground in liquid nitrogen and resuspended in protein extraction buffer (25 mM Tris-HCl, pH 7.5; 150 mM NaCl; 0.1% IGEPAL CA-630 and Roche cOmplete protease inhibitor cocktail, EDTA-free) in a 1:1 (w/v) ratio, followed by a centrifuging step to discard the plant debris. Protein concentration was determined with Quick Start Bradford 1x reagent (Bio-Rad) and adjusted to 5 mg/mL by adding extraction buffer. The lysate was then split into different reaction tubes and incubated with the respective chemical (DMSO mock, IAA or BA) at the indicated concentrations for 30 min at room temperature with slow mixing. The treated aliquots were further aliquoted and mixed with different dilutions of Pronase (Roche), prepared in Pronase buffer (25 mM Tris-HCl, pH 7.5 and 150 mM NaCl) to achieve the aimed for ratio of total enzyme to total protein. After incubation for 30 min at room temperature, the proteolytic digestion was terminated by adding protease inhibitor cocktail (cOmplete, Roche) and the samples were kept on ice for 10 min. The protein samples were then mixed with 4x NuPAGE LDS sample buffer (Invitrogen) and heated at 80°C for 10 min. The protein samples were analysed by Western blot (TGX gels Bio-Rad, TurboTransfer Bio-Rad PVDF membranes), visualized using an anti-FLAG-HRP antibody (1:1000 – Sigma A8592). HRP activity was detected using the Supersignal Western Detection Reagents (Thermo Scientific) and imaged with a GE Healthcare Amersham 600 RGB system.

Protein production

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The ES domain, ES388-594, protein was isotopically labelled in preparation for NMR analysis. The ES domain was expressed for as a fusion protein with a 6x Histidine tag in minimal media with 15N ammonium chloride. The 15N isotope labelling of the expressed protein involved a 125-fold dilution of cell culture in enriched growth media into minimal media with 15N ammonium chloride and grown for 16 hr (37 °C / 200 rpm); followed by a further 40-fold dilution into minimal media for the final period of cell growth and protein expression (induced with L-arabinose 0.2 % w/v / 18 °C / 200 rpm and grown for a further 12 hr). The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0, NaCl 500 mM and either no-imidazole or 500 mM imidazole for wash and elution buffers respectively. The majority of the non-specifically bound protein was removed by passing 20 mL of the wash buffer through the columns. The protein eluted on a gradient of increasing imidazole concentration of up to 30% elution buffer over 20 mL.

For ITC, the ES388-594 protein was grown in LB medium, cells harvested and lysed as described above. After centrifugation of the sonicated cell suspension, the pellet was resuspended and washed in Wash Buffer (50 mM Tris-HCl, pH 8.0; 0.1 mM EDTA; 5% (v/v) Glycerol; 0.1 mM DTT; 50 mM NaCl; 2% (w/v) NaDOC) for 1 hour at 4°C followed by centrifugation (11,000 g for 20 min at 4 °C). The resulting pellet was again washed in Wash Buffer and centrifuged as described previously. The pellet was resuspended in 20mL of Solubilisation Buffer (50 mM Tris-HCl, pH 8.0; 0.1 mM EDTA; 5% (v/v) Glycerol; 0.1 mM DTT; 50 mM NaCl; 0.25% Sarkosyl (N-Lauroyl sarcosine) and stirred at 4°C for 1h. Subsequently the resuspension was dialysed overnight in 2L Dialysis Buffer (50 mM Tris-HCl, pH 8.0; 0.1 mM EDTA; 5% (v/v) Glycerol; 0.1 mM DTT; 50 mM NaCl). The Dialysis Buffer was exchanged for fresh Dialysis Buffer after at least 2 hours of dialysis. After dialysis the lysate was centrifuged (11,000 g for 20 min at 4 °C). The resulting supernatant contained the required protein which was concentrated to 2 mL using Amicon Ultra-15 centrifugal filters with a 10kDa cut-off (Millipore (UK) Ltd.) and further purified using size exclusion chromatography. The protein was eluted in Chromatography Buffer and concentrated to 2 mL as previously described. Protein concentration was determined with Quick Start Bradford 1x reagent (Bio-Rad) and adjusted using Chromatography Buffer.

HSQC NMR

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The ES domain, ES388-594, protein was analysed by NMR at 5°C under reducing conditions (DTT 10 mM), buffered at pH 8.0 (Tris 20 mM). 1H-15N HSQC was performed at 950 MHz, TCI probe, Bruker following the parameters described in Figure 1—figure supplement 1. Concentrations used were 50 µM ES388-594 and 500 µM IAA ligand (i.e. 1:10).

Isothermal titration calorimetry (ITC)

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ITC was carried out on a MicroCal PEAQ-ITC (Malvern) at 25°C in a Buffer A (sodium phosphate 20 mM, pH 8.0; NaCl 500 mM). Ligand (2 mM IAA) was injected (19 × 4.0 μl) at 150 s intervals into the stirred (500 rpm) calorimeter cell (volume 270 μl) containing 50 µM ES388-594 protein. Titration of Buffer A into 50 µM ES388-594 protein and IAA (2 mM) into Buffer A served as negative controls. Measurements of the binding affinity of all the titration data were analysed using the MicroCal Software (Malvern).

Accessions

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ETT, AT2G33860; TPL, AT1G15750; TPR1, AT1G80490, TPR2, AT3G16830; TPR3, AT5G27030; TPR4, AT3G15880; HDA6, AT5G63110; HDA19, AT4G38130; HEC1, AT5G67060; PID, AT2G34650; WUS, AT2G17950.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 1, 2, 3, 4 and 5.

References

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    2. Feldman LJ
    3. Zambryski PC
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    Auxin and ETTIN in Arabidopsis gynoecium morphogenesis
    Development 127:3877–3888.
    1. Sessions A
    2. Nemhauser JL
    3. McColl A
    4. Roe JL
    5. Feldmann KA
    6. Zambryski PC
    (1997)
    ETTIN patterns the Arabidopsis floral meristem and reproductive organs
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    Arabidopsis gynoecium structure in the wild and in ettin mutants
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Article and author information

Author details

  1. André Kuhn

    Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
    Contribution
    Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2144-8413

  2. Sigurd Ramans Harborough

    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
    Contribution
    Data curation, Formal analysis, Validation, Investigation, Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0361-5647

  3. Heather M McLaughlin

    Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
    Contribution
    Data curation, Formal analysis, Validation, Investigation, Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3020-7964

  4. Bhavani Natarajan

    Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
    Contribution
    Data curation, Investigation, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6852-7130

  5. Inge Verstraeten

    Institute of Science and Technology, Klosterneuburg, Austria
    Contribution
    Data curation, Investigation, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7241-2328

  6. Jiří Friml

    Institute of Science and Technology, Klosterneuburg, Austria
    Contribution
    Formal analysis, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8302-7596

  7. Stefan Kepinski

    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
    Contribution
    Formal analysis, Funding acquisition
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9819-5034

  8. Lars Østergaard

    Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, United Kingdom
    Contribution
    Conceptualization, Formal analysis, Supervision, Funding acquisition, Project administration
    For correspondence
    lars.ostergaard@jic.ac.uk
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8497-7657

Funding

Biotechnology and Biological Sciences Research Council (BB/S002901/1)

  • Lars Østergaard

Biotechnology and Biological Sciences Research Council (BB/L010623/1)

  • Stefan Kepinski

Biotechnology and Biological Sciences Research Council (BB/M011216/1)

  • André Kuhn

Biotechnology and Biological Sciences Research Council (BB/P013511/1)

  • Lars Østergaard

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We are grateful to Yuli Ding, Yang Dong, Emilie Knight, Mikhaela Neequaye, Nicola Stacey, Sophia Stavnstrup, Billy Tasker-Brown for critical comments on the manuscript, to Keiko Torii and Shinya Hagihara for the ccvTIR1 line and cvxIAA ligand, to Rebecca Mosher for assistance with the phylogenetic analysis of ETT protein sequences, to Thomas Laux for TPL::TPL-GFP and 35S::HDA19-GFP lines and to Salomé Prat for 35S::TPL-HA construct. Our gratitude also goes to Felix Ramos-León, Kelley Gallagher and Mark Buttner for assistance with protein production, Clare Stevenson for ITC support, the Norwich Research Park Bioimaging for skillful assistance and the NMR facility in the Astbury Centre, Faculty of Biological Sciences for access to the 950 MHz and 600 MHz spectrometers funded by the University of Leeds. We thank Arnout Kalverda for assistance with analysing the NMR data.

Copyright

© 2020, Kuhn et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. André Kuhn
  2. Sigurd Ramans Harborough
  3. Heather M McLaughlin
  4. Bhavani Natarajan
  5. Inge Verstraeten
  6. Jiří Friml
  7. Stefan Kepinski
  8. Lars Østergaard
(2020)
Direct ETTIN-auxin interaction controls chromatin states in gynoecium development
eLife 9:e51787.
https://doi.org/10.7554/eLife.51787

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https://doi.org/10.7554/eLife.51787

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