Random access parallel microscopy
- McGill University, Canada
- University of Exeter, United Kingdom
- Université Laval, Canada
Abstract
We introduce a random access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and Caenorhabditis elegans (C. elegans) preparations.
Data availability
All data generated during this study are included in the manuscript and supporting files
Article and author information
Author details
Alexander D. Corbett
Funding
National Science and Engineering Research Council of Canada (RGPIN-2018-05346)
- Gil Bub
National Science and Engineering Research Council of Canada (RGPIN-2016-05962)
- Simon Thibault
Funders support enabled acquisition of equipment used in this study and also funded summer student stipends for undergraduate authors.
Reviewing Editor
- Jonathan Ewbank, Aix Marseille Université, INSERM, CNRS, France
Version history
- Received: February 27, 2020
- Accepted: January 11, 2021
- Accepted Manuscript published: January 12, 2021 (version 1)
- Accepted Manuscript updated: January 15, 2021 (version 2)
- Version of Record published: January 28, 2021 (version 3)
Copyright
© 2021, Ashraf et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Random access parallel microscopy
eLife 10:e56426.
https://doi.org/10.7554/eLife.56426
Further reading
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