1. Evolutionary Biology
  2. Genetics and Genomics
Download icon

Homing Genetic Elements: Mobile DNAs and switching mating types in yeast

  1. Laura N Rusche  Is a corresponding author
  1. Department of Biological Science, University of Buffalo, The State University of New York, United States
Insight
Cite this article as: eLife 2020;9:e58403 doi: 10.7554/eLife.58403
1 figure

Figures

The missing link in the evolution of HO.

(A) The HO gene is structurally similar to two other mobile DNA elements, VDE and WHO. All three genes have an intein-like domain (blue rectangle) that includes an endonuclease domain: in HO this domain cuts DNA at the MAT locus. The WHO and HO genes also have a zinc finger domain (green). The WHO gene is also located next to its target, the FBA1 gene. (B) Coughlan et al. propose that the WHO gene homes into the FBA1 gene as follows. First, the enzyme encoded by the WHO gene (blue and green ovals) cuts a target gene, represented as FBA1’ (dark gray box), which is not flanked by WHO. Next, the break is repaired using a template of the FBA1 gene (medium gray) which is adjacent to the WHO gene and a fragment of FBA1 (A1, light gray). The template sequence (including the WHO gene) is copied and inserted into the cleaved site of the target gene. Recombination between the target FBA1’ gene and the template FBA1 gene creates a new FBA1 gene (dark and medium gray) and FBA1 fragment (light gray and dark gray), which sit on either side of the WHO gene. The newly recombined FBA1 gene is no longer susceptible to cleavage by the enzyme encoded by WHO (represented by the T symbol).

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)