Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring AMR bacterial pneumonia
- University of Bristol, United Kingdom
- North Bristol NHS Trust, United Kingdom
- Bristol Veterinary School in the Faculty of Health Sciences, United Kingdom
- Imperial College London, United Kingdom
- University of Birmingham, United Kingdom
Abstract
Here we describe the case of a COVID-19 patient who developed recurring ventilator-associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed massive and escalating levels of T-cell activation. These were both SARS-CoV-2 and P. aeruginosa specific, and bystander activated, which may have contributed to this patient's persistent symptoms and radiological changes.
Data availability
Human-filtered sequencing data for this study have been deposited in the European Nucleotide Archive (ENA) at EMBL-EBI under accession PRJEB40239.
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Metagenomic analysis of respiratory samples from a COVID-19 ICU patientEuropean Nucleotide Archive, PRJEB40239.
Article and author information
Author details
Michaela Gregorova
Mahableshwar Albur
Funding
Southmead Hospital Charity
- Fergus Hamilton
Wellcome Trust (212258/Z/18/Z)
- Ruth C Massey
Elizabeth Blackwell Institute
- Laura Rivino
UKRI (MR/S019553/1)
- Rosemary J Boyton
UKRI (MR/R02622X/1)
- Daniel M Altmann
Cystic Fibrosis Trust (CF Trust SRC 015)
- David K Butler
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Human subjects: The patient was enrolled onto the DISCOVER study (Diagnostic and Severity markers of COVID-19 to Enable Rapid triage study), a single centre prospective study recruiting consecutive patients admitted with COVID-19, from 30.03.2020 until present (Ethics approval via South Yorkshire REC: 20/YH/0121, CRN approval no: 45469). Blood/serum samples from pre-pandemic healthy controls and asymptomatic healthy controls were obtained under the Bristol Biobank (NHS Research Ethics Committee approval ref 14/WA/1253).
Reviewing Editor
- Anurag Agrawal, CSIR Institute of Genomics and Integrative Biology, India
Publication history
- Received: September 24, 2020
- Accepted: December 16, 2020
- Accepted Manuscript published: December 17, 2020 (version 1)
- Version of Record published: December 31, 2020 (version 2)
Copyright
© 2020, Gregorova et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring AMR bacterial pneumonia
eLife 9:e63430.
https://doi.org/10.7554/eLife.63430
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