7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome
Abstract
Defective 3β-hydroxysterol-Δ7 -reductase (DHCR7) in the developmental disorder, Smith-Lemli-Opitz syndrome (SLOS), results in a deficiency in cholesterol and accumulation of its precursor, 7-dehydrocholesterol (7-DHC). Here, we show that loss of DHCR7 causes accumulation of 7-DHC-derived oxysterol metabolites, premature neurogenesis from murine or human cortical neural precursors, and depletion of the cortical precursor pool, both in vitro and in vivo. We found that a major oxysterol, 3β,5α-dihydroxycholest-7-en-6-one (DHCEO), mediates these effects by initiating crosstalk between glucocorticoid receptor (GR) and neurotrophin receptor kinase TrkB. Either loss of DHCR7 or direct exposure to DHCEO causes hyperactivation of GR and TrkB and their downstream MEK-ERK-C/EBP signaling pathway in cortical neural precursors. Moreover, direct inhibition of GR activation with an antagonist or inhibition of DHCEO accumulation with antioxidants rescues the premature neurogenesis phenotype caused by the loss of DHCR7. These results suggest that GR could be a new therapeutic target against the neurological defects observed in SLOS.
Editor's evaluation
This is an important paper that provides a conceptual framework for understanding how altered lipid metabolism can impact brain development. The authors use mouse models and human iPSCs to provide a convincing mechanistic explanation of how mutations in a key enzyme in cholesterol biosynthesis lead to a neurodevelopmental disorder.
https://doi.org/10.7554/eLife.67141.sa0Introduction
Brain is rich in cholesterol, contributing to 25% of total cholesterol in the human body, and nearly all cholesterol in the brain is synthesized locally (Dietschy and Turley, 2004). Therefore, dysregulation of cholesterol metabolism in CNS can potentially cause significant defects in CNS development and functions. Indeed, enzymatic deficiencies in cholesterol biosynthesis cause a number of inherited diseases with severe neurodevelopmental phenotypes (Porter and Herman, 2011).
Smith-Lemli Opitz syndrome (SLOS) is an autosomal recessive, neurological and developmental disorder characterized by multiple developmental defects, such as distinctive facial features, cleft palate, microcephaly and holoprosencephaly, as well as severe intellectual impairment and behavioral problems (Porter and Herman, 2011; Thurm et al., 2016). Notably, SLOS patients display a high incidence (>50%) of autism spectrum disorders (Bukelis et al., 2007; Sikora et al., 2006; Tierney et al., 2006). SLOS is caused by mutations in the 3β-hydroxysterol-Δ7 -reductase gene (DHCR7), which encodes the enzyme that converts 7-dehydrocholesterol (7-DHC) to cholesterol in the final step of the cholesterol biosynthesis pathway (Fitzky et al., 1998; Tint et al., 1994; Wassif et al., 1998). Defective DHCR7 resulting from the mutations leads to deficiency in cholesterol and accumulation of 7-DHC in tissues and fluids of affected individuals (Tint et al., 1994; Tint et al., 1995). 7-DHC was found to be highly reactive toward free radical oxidation, leading to the formation of its oxidative metabolites, that is oxysterols (Xu et al., 2009; Xu et al., 2010; Xu et al., 2013; Xu et al., 2011). 7-DHC-derived oxysterols can exert cytotoxicity in neuronal cells, induce gene expression changes, and increase formation of dendritic arborization from cortical neurons (Korade et al., 2010; Xu et al., 2012). Interestingly, increased dendrite and axon formation has also been observed in neurons isolated from Dhcr7-KO (Dhcr7-/-) mouse brain (Jiang et al., 2010). These studies suggest that 7-DHC-derived oxysterols may be underlying the alterations in neuronal processes in SLOS.
Cholesterol, 7-DHC, and oxysterols derived from both have been found to play important roles in modulating signaling pathways in developing tissues and organs, such as Hedgehog (Hh) and Wnt signaling pathways (Byrne et al., 2016; Corcoran and Scott, 2006; Huang et al., 2018; Myers et al., 2017; Porter et al., 1996; Raleigh et al., 2018). Related to SLOS, ring-B oxysterols derived from 7-DHC oxidation inhibit Smo in the Hh pathway (Sever et al., 2016). On the other hand, cholesterol was recently found to selectively activate canonical Wnt signaling over non-canonical Wnt signaling (Sheng et al., 2014). However, in human induced pluripotent stem cells (hiPSCs) derived from SLOS patient fibroblasts, accumulation of 7-DHC was found to inhibit Wnt/β-catenin pathway, which contributes to the precocious neuronal specification in SLOS neural progenitors (Francis et al., 2016). Depending on the position of the oxidation, oxysterols have also been shown to bind and activate other signaling molecules and nuclear receptors, including estrogen receptors, liver X receptors, and glucocorticoid receptor (GR), and thus, play important roles in neurodevelopment and diseases (DuSell et al., 2008; Theofilopoulos et al., 2013; Voisin et al., 2017). However, it is unknown how alteration of sterol composition influences neural stem cell/progenitor behaviors during cortical development, and it remains elusive whether neural defects of SLOS are due to deficiency in cholesterol or accumulation of 7-DHC or its oxysterols.
Here, we examine the effects of DHCR7 mutations in developing neural precursors, focusing on the cerebral cortex. We demonstrate that 7-DHC-derived oxysterols start to accumulate at embryonic day 12.5 (E12.5) and continue to increase at E14.5 and E16.5, and that loss of DHCR7 causes decreased proliferation and self-renewal of mouse cortical neural precursors and aberrant premature neurogenesis in both mouse and human neural progenitor cells (NPCs). We then provide evidence that a 7-DHC-derived oxysterol, 3β,5α-dihydroxycholest-7-en-6-one (DHCEO), activates GR and the downstream receptor tyrosine kinases (RTKs)-mediated neurogenic signaling through TrkB, and in doing so, promotes premature NPC differentiation and perturbs neuronal positioning. Either inhibition of GR activation with an antagonist or inhibition of DHCEO accumulation with antioxidants rescues the premature neurogenesis defect.
Results
Dhcr7 is expressed in embryonic cortical precursors and neurons of developing murine cortex
To understand the role of Dhcr7 in neural development, we studied murine developing cortical precursors during embryonic neurogenesis. We first analyzed the expression of Dhcr7 mRNA during mouse cortical development. RT-PCR analysis showed expression of Dhcr7 in embryonic day 11 (E11) through postnatal day 0 (P0) cortex (Figure 1A). Quantitative PCR (qPCR) also suggested the stable expression of Dhcr7 from E11 to E18 (Figure 1B). Western blot analysis further confirmed the presence of Dhcr7 protein in the developing cortex (Figure 1C). Immunostaining showed that Dhcr7 was broadly expressed in the developing cortex and was detectable in precursors in the E12 ventricular and subventricular zones (VZ/SVZ) (Figure 1D). Immunostaining of cortical sections of Dhcr7-/- mouse brain at E12 with this particular Dhcr7 antibody confirmed the antibody specificity against Dhcr7. To further establish the expression of Dhcr7 in neural lineage cells, we analyzed cortical precursor cultures from E12.5, which consists of proliferating radial glial cells that generate neurons in culture (Figure 1E). In the E12.5 cortical precursor cultures, Dhcr7 + cells were co-labeled with the precursor markers, Sox2 and Pax6. Dhcr7 was also co-labeled with the neuronal marker, βIII-tubulin, and the neural lineage marker, Nestin. The results suggested that Dhcr7 was consistently expressed in neural lineage cells, which is consistent with our in vivo analysis.
Loss of Dhcr7 alleles causes decreased proliferation and increased neurogenesis in murine and human NPCs
SLOS is characterized by neurodevelopmental defects, such as microcephaly. To ask if Dhcr7 plays important roles in neural precursor (progenitor) development as seen in human patients with SLOS, we intercrossed Dhcr7+/- mice and prepared single embryo cultures from E12.5 Dhcr7-/- (knockout or KO) or Dhcr7+/+ embryos. Cultures were immunostained for Ki67 and βIII-tubulin 3 days after plating, which revealed that loss of Dhcr7 caused a significant decrease in the proportion of Ki67 + and Pax6+precursors whereas increased the proportion of βIII-tubulin+neurons (Figure 1F–I). Immunostaining for cleaved caspase 3 (CC3) showed that the loss of Dhcr7 alleles did not affect the survival of cortical precursor cells in culture (Figure 1J). These results suggested that loss of Dhcr7 leads to premature neurogenesis and decreased proliferation of cortical precursors.
To determine if Dhcr7 is important for the proliferation and self-renewal of cortical precursors, we performed neurosphere assays, which assess if sphere-forming precursors can self-renew and generate new spheres. E13.5 cortical precursors from Dhcr7-/- and Dhcr7+/+ embryos were cultured in the presence of FGF2 and EGF. The number and diameter of spheres were measured 7 days post-plating (Figure 1K–O). Significantly fewer number of and smaller neurospheres were generated from Dhcr7-/- embryonic cortices compared to its Dhcr7+/+ littermates (Figure 1L–M). These results were consistent with the reduced proportion of Ki67 + precursors in the adherent cultures. Following the formation of the primary neurospheres, these spheres were triturated and re-plated at an equal density to form the secondary neurospheres. The results showed that there was an approximately twofold decrease in the number and diameter of secondary spheres from Dhcr7-/- cortical precursors relative to Dhcr7+/+ (Figure 1N–O). Thus, the loss of Dhcr7 alleles disrupts the proliferation and self-renewal of cortical precursors.
We then asked whether DHCR7 was also necessary for the neurogenesis of human stem cell-derived NPCs. To examine the function of DHCR7, we generated human induced pluripotent stem cells (hiPSCs) from two lines of SLOS patient fibroblasts and one line of unaffected (Control) human fibroblasts and verified their pluripotency and stemness as described in Methods (Figure 1—figure supplement 1; Okita et al., 2011; Yu et al., 2007). The SLOS hiPSCs were then differentiated into hNPCs as described previously. Immunoreactivity of DHCR7 was detected in almost all Control hNPCs in these cultures with Sox2 + and Nestin + cells (Figure 1P). To examine neural differentiation, the SLOS and Control NPCs were differentiated in cultures and immunostained 4 days later for Ki67 and βIII-tubulin for proliferating precursors and newborn neurons, respectively (Figure 1). Notably, the proportion of Ki67 + precursors was significantly decreased whereas the proportion of βIII-tubulin+neurons were increased in SLOS NPC cultures, similar to the phenotype seen in murine cortical precursors from Dhcr7-/- embryos. Taken together, DHCR7 is involved in the proliferation and differentiation of human NPCs.
Cholesterol precursor 7-DHC and 7-DHC-derived Oxysterols are accumulated in Dhcr7-/- developing cortex and human NPCs
7-DHC is highly susceptible to free radical oxidation (Xu et al., 2009), which leads to the formation of numerous oxysterols in cells and tissues (Xu et al., 2013; Xu et al., 2011; Figure 2A). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed on cortices from Dhcr7-/- and Dhcr7+/+ embryos during cortical development. These analyses revealed significant accumulation of 7-DHC and reduction of cholesterol in the embryonic cortices from Dhcr7-/- embryos throughout cortical development from E12.5 to E16.5 (Figure 2B). Increased levels of 7-dehydrodesmosterol (7-DHD), the precursor to desmosterol via Dhcr7, and 8-dehydrocholesterol (8-DHC) and decreased levels of desmosterol in Dhcr7-/- cortices are also consistent with the loss of the function of Dhcr7. Furthermore, 7-DHC oxysterols, such as DHCEO, 4α-hydroxy-7-DHC (4α-OH-7DHC), and 4β-hydroxy-7-DHC (4β-OH-7DHC) (Xu et al., 2013; Xu et al., 2011) showed substantial accumulation in the Dhcr7-/- cortices, reaching up to 4–8 ng/mg of tissue, throughout the development (Figure 2A and C, and Figure 2—figure supplement 1). Note that 4 ng/mg of tissue would translate into approximately 10 μM assuming the density of the brain is 1 g/mL. On the other hand, cholesterol-derived oxysterols, such as 24-keto-Chol, 24,25-epoxy-Chol, 24- or 25-OH-Chol, 7-OH-Chol, and 7-keto-Chol decreased or did not change significantly in Dhcr7-/- cortices relative to Dhcr7+/+ cortices.
Furthermore, significant accumulation of 7-DHC and 7-DHC-derived oxysterols and reduction of cholesterol were also found in both SLOS patient-derived hiPSCs and NPCs relative to Controls (Figure 2—figure supplement 2). Taken together, DHCR7 mutations led to the accumulation of 7-DHC and 7-DHC-derived oxysterols in murine and human NPCs.
Knockdown (KD) of Dhcr7 causes increased neurogenesis and depletion of cycling precursors in murine and human NPC cultures
To examine the potential role of Dhcr7, we generated three Dhcr7 short hairpin RNAs (shRNAs)-EGFP reporters and transfected them into 293T cells along with murine Dhcr7 cDNA-expressing plasmids. We found that Dhcr7 shRNA2 was the most effective among those shRNAs and was chosen to examine Dhcr7 function during neurogenesis from E12.5 cortical precursors in culture (Figure 3A). When cortical precursors were transfected with Dhcr7 shRNA2-EGFP, the shRNA significantly decreased the percentage of transfected cells expressing Dhcr7 (Figure 3B–C). Dhcr7 KD led to a significant decrease in the proliferation of cortical precursors as measured by Ki67 immunoreactivity 3 days post-transfection or by adding 5-ethynyl-2-deoxyuridine (EdU) to culture 1 day post-transfection and immunostaining for EdU 2 days later (Figure 3D–F). The significant decrease in precursor proliferation was caused by a decrease in Pax6 +radial precursors (Figure 3G). Furthermore, Dhcr7 KD led to a significant increase of βIII-tubulin+neurons 3 days post-transfection, but it did not affect cell survival as examined by immunostaining for CC3 (Figure 3D, H and I).
Dhcr7 KD also decreased self-renewal of the radial precursor as demonstrated by clonal analysis with piggyBac (PB) transposon, which permanently labels precursors and their progeny. Cortical precursors were transfected with PB transposase and PB-Dhcr7 shRNA2-EGFP or control shRNA-EGFP, and cultures were immunostained 3 days post-transfection for EGFP, the precursor markers, Sox2, and βIII-tubulin (Figure 3J–M). KD of Dhcr7 reduced EGFP + multicellular clones (Figure 3K) whereas neuron-only (βIII-tubulin+) clones were increased (Figure 3L). Furthermore, the number of precursors in clones containing at least one Sox2 + cell is decreased (Figure 3M).
To ensure that these changes were Dhcr7 shRNA-dependent, we performed rescue experiments using human DHCR7 cDNA that is resistant to the murine Dhcr7 shRNA (Figure 3—figure supplement 1). The murine shRNA did not affect the expression of human DHCR7 cDNA as confirmed by co-transfection of the murine shRNA and human DHCR7 cDNA in 293T cells and western blot analysis 2 days later (Figure 3—figure supplement 1A). Furthermore, precursors were co-transfected with murine Dhcr7 shRNA-EGFP or control shRNA-EGFP +/- human DHCR7 cDNA and immunostained 3 days later for Ki67 or βIII-tubulin (Figure 3—figure supplement 1B, C). The human DHCR7 cDNA showed significant rescue of the murine Dhcr7 shRNA KD phenotypes, confirming that the neurogenesis and proliferation phenotype is Dhcr7 shRNA-dependent.
Finally, we asked whether DHCR7 also plays a role in human iPSC-derived NPCs (Figure 3N–R). To examine the function of DHCR7, we generated a human-targeted DHCR7 shRNA-EGFP and confirmed its efficiency by transfecting it into 293T cells along with human DHCR7 expressing vectors (Figure 3N). Human NPCs were transfected with this shRNA-EGFP and analyzed by immunostaining 4 days later (Figure 3O), which showed that the KD of human DHCR7 reduced EGFP + and Ki67 + proliferating precursors whereas increased EGFP + and βIII-tubulin+newborn neurons (Figure 3P–R), similar to those observed in murine cortical precursors and SLOS hNPCs.
7-DHC derived oxysterols lead to similar neurogenic defects as loss of Dhcr7 in murine cortical precursors in vitro
Oxysterols have been found to influence biological processes, including proliferation, differentiation, and cell survival (DuSell et al., 2008; Theofilopoulos et al., 2013; Voisin et al., 2017). Thus, we asked whether 7-DHC-derived oxysterols play regulatory roles in cortical precursor biology. To assess the potential effect of these 7-DHC-derived oxysterols on cortical precursor behaviors, cortical precursors from E12.5 Dhcr7+/+ embryos were cultured and treated with different concentrations of individual oxysterols for 3 days, followed by immunostaining for Ki67, βIII-tubulin, and CC3 (Figure 4A). Notably, DHCEO-treated cortical precursors showed a significant increase in the proportion of βIII-tubulin+newborn neurons and a significant decrease in the proportion of Ki67 + precursors (Figure 4B–C). This effect of DHCEO was observed in a dose-dependent manner up to 3.5 µM, which is roughly the concentration of DHCEO in the brains of P0 Dhcr7-/- mice (Xu et al., 2011). There was no significant change in cell death/survival with DHCEO treatment (Figure 4D). The other oxysterols showed a notable toxic effect at a concentration of 5 µM as indicated by increased CC3 + cells (Figure 4G, J and M). Note that the level of 7k-DHC is the lowest among the oxysterols, only reaching 0.9 ng/mg of tissue (equivalent to 2.3 µM), so we do not expect the highest concentration examined, 5 µM, for this oxysterol is relevant to the neurogenic phenotype. Interestingly, 4α-OH-7DHC treatment at a concentration of 2 µM also displayed significant increases in βIII-tubulin+neurons and decreases in Ki67 + precursors without significant changes in cell death/survival (Figure 4H–J). However, at 5 µM, CC3 + cells were significantly elevated at the expense of βIII-tubulin+neurons (Figure 4J). To summarize, treatment with DHCEO at physiologically relevant concentrations completely replicated the premature neurogenic phenotype observed in murine and human SLOS NPCs while 4α-OH-7DHC may also contribute to the phenotype despite its toxicity at high concentrations.
Loss of Dhcr7 causes premature differentiation and reduced thickness of cortical layers in vivo
We asked whether loss of Dhcr7 disrupted cortical development in vivo as was observed in vitro. To examine this, cortical sections from Dhcr7-/- mice at E18.5 were immunostained for the expression of well-defined cortical layer markers: the layer 2–4 marker Brn2, the layer 5 marker CTIP2, and the layer 6 marker Tbr1 (Molyneaux et al., 2007; Figure 5A). The analyses showed loss of Dhcr7 led to a reduction of all cortical layers, resulting in the reduction of the overall size of the neocortex (Figure 5B, C). To further evaluate the effects of Dhcr7-/- in the fate decision of cortical precursors during corticogenesis, we immunostained cortical sections from Dhcr7-/- mice at E14.5 and E15.5 for Tbr1 and Satb2, which label early-born and late-born cortical neurons, respectively (Figure 5D–E). The analyses showed a significant increase of Tbr1 + neurons and Satb2 + neurons in the cortical section from Dhcr7-/- mice at E14.5 and E15.5, respectively (Figure 5F–G). This aberrant increased production of neurons with loss of Dhcr7 potentially alters the number and localization of developing neurons in the cortex. To test this hypothesis, we injected pregnant dams with EdU at E12.5 to label proliferating radial precursors and analyzed Dhcr7+/+ and Dhcr7-/- littermates 3 days later at E15.5 to identify the locations of EdU-labeled cells in the cortex (Figure 5H–I). The majority of EdU-labeled cells migrated into the cortical plate in the KO mice, whereas EdU-labeled cells were scattered throughout the intermediate zone (IZ) and the cortical plates in the Dhcr7+/+ littermates. These observations suggest premature increases in precursor differentiation, which raises the possibility of activating major neurogenic signaling pathways during corticogenesis. RTKs and their downstream targets are known to play important roles in precursor proliferation and differentiation in the developing cortex, and TrkB, one of the major RTKs expressed in cortical precursors, regulates proliferation and differentiation into neurons by activating the MEK-ERK-C/EBP pathway (Barnabé-Heider and Miller, 2003; Bartkowska et al., 2007; Ménard et al., 2002). Thus, we examined the activity of TrkB-MEK-ERK-C/EBP pathway by western blot with antibodies for phosphorylated activated MEK1 and C/EBPβ at E15.5 of Dhcr7-/- cortices (Figure 5J–K). This analysis showed that relative to total TrkB, MEK1, and C/EBPβ levels, their phosphorylated forms increased in Dhcr7-/- cortices (Figure 5K). This observation suggests that loss of Dhcr7 can dysregulate an RTK-dependent signaling pathway that is critical for neural precursor proliferation and differentiation.
The above observations indicate premature precursor differentiation at the expense of depleting the proliferative precursor pool. To examine this hypothesis, pregnant dams were injected with Edu at different time points of cortical development, and Dhcr7+/+ and Dhcr7-/- littermates were analyzed 18 hr later to quantify the number of cycling progenitors that have exited the cell cycles (Figure 6A, B). Cells that had left the cell cycle were stained as Edu + and Ki67-, whereas cells that re-entered the cell cycle were stained as Edu + and Ki67+. The index of the cell cycle exit is defined by the percentage of Edu+/Ki67- cells in total Edu + cells. The index showed that the number of cells exiting the cell cycle in Dhcr7-/- cortices was significantly increased at E13.5 and E14.5 compared with Dhcr7+/+ cortices, indicating that loss of Dhcr7 increased cell cycle exit. Increased cell cycle exit of cortical precursors can potentially arise from changes in proliferation. To test this possibility, pregnant dams were pulsed with Edu for 2 hr to label all cells in S-phase and the fraction of cortical precursors in the S-phase, a proliferating index from E13.5 to E15.5 in Dhcr7-/- and Dhcr7+/+ cortices, was quantified (Figure 6C–D). The proliferating index was determined by the percentage of Edu + and Ki67 + cells out of total Ki67 + cells, which provides an estimate of cell cycle length because the length of the S phase remains relatively constant in mammalian cells, whereas the length of the G1 phase regulates proliferation (DiSalvo et al., 1995). The analyses showed a significant decrease in the proliferating index in Dhcr7-/- cortices compared to Dhcr7+/+ cortices from E13.5 to E15.5 (Figure 6C–D), indicating slower cycle progression and longer cycle length in Dhcr7-/- cortices (Chenn and Walsh, 2002). Indeed, this accelerated depletion of the progenitor pool in Dhcr7-/- cortices results in decreases in the size of VZ/SVZ compared to Dhcr7+/+ cortices from E13.5 to E15.5 (Figure 6E–F). Taken together, these results suggest that loss of Dhcr7 leads to a decrease in the overall number of cortical precursors with increased cell cycle exit and decreased proliferation index via activation of the RTK-mediated MEK-ERK-C/EBP pathway.
Perturbation in DHCR7 causes gene expression changes in neurogenic pathways in human NPCs
To ask whether DHCR7 mutations influence the NPC biology, we aim to compare gene expression profiles between hNPCs with DHCR7 mutations versus hNPCs from healthy individuals. To do so, we expanded the hNPCs derived from hiPSCs in the presence of essential growth factors and carried out RNA sequencing (RNAseq) on these cells. Relative to Control hNPCs, 2357 genes were significantly differentially expressed in SLOS hNPCs (1072 upregulated and 1285 downregulated genes with adjusted p-value < 0.05), distinguishing the SLOS and Control precursors (Figure 7A; Supplementary file 6 for RNAseq data). Gene ontology and pathway analysis found that the PI3K-Akt signaling pathway, MAPK signaling, and Ras signaling pathways are among those with the highest p-values (Figure 7B–C; Supplementary file 5). Notably, MAP2K1(also known as MEK1) was found to be upregulated in SLOS patient-derived hNPCs. MAP2K1 is a key signaling molecule in MAPK and Ras signaling pathways, which are known to play important roles in precursor proliferation and differentiation (Bonni et al., 1999; Ménard et al., 2002; Yang et al., 2013), consistent with the phenotypes that were observed in Dhcr7-/- mice and SLOS patient-derived hNPCs in vitro.
7-DHC-derived oxysterol, DHCEO, activates cortical neurogenesis via activation of the glucocorticoid receptor (GR) and inhibition of GR or inhibition of the formation of DHCEO rescues the neurogenic defects in SLOS NPCs
As shown in Figure 4, treatment of WT mouse NPCs with 7-DHC-derived oxysterols, particularly DHCEO, can replicate the same aberrant premature neurogenesis observed in SLOS NPCs. However, the mechanism of action of DHCEO remains unknown. Interestingly, 6-oxo-cholestan-3β,5α-diol (OCDO), a cholesterol-derived oxysterol that is structurally similar to DHCEO, is shown to bind and activate glucocorticoid receptor (GR) (Voisin et al., 2017; Figure 8—figure supplement 1A). Additionally, GR activation has been shown to activate TrkB (Jeanneteau et al., 2008), which leads to further activation of the RTK-mediated MEK-ERK pathway (Barnabé-Heider and Miller, 2003; Bartkowska et al., 2007; Ménard et al., 2002). TrkB and RTK-mediated MEK-ERK pathway are necessary for neurogenesis during embryonic cortical development. Thus, we asked whether DHCEO can activate the GR and further lead to the activation of the MEK-ERK neurogenic pathway. To investigate this, we first examined if GR was phosphorylated and activated in the embryonic cortices from Dhcr7-/- and Dhcr7+/+ embryos at E15.5 in addition to TrkB, MEK, and C/EBPβ shown in Figure 5J. Western blots demonstrated that phosphorylation of GR was indeed increased in Dhcr7-/- cortices compared to Dhcr7+/+ cortices (Figure 8A).
-
Figure 8—source data 1
- https://cdn.elifesciences.org/articles/67141/elife-67141-fig8-data1-v2.pdf
-
Figure 8—source data 2
- https://cdn.elifesciences.org/articles/67141/elife-67141-fig8-data2-v2.pdf
-
Figure 8—source data 3
- https://cdn.elifesciences.org/articles/67141/elife-67141-fig8-data3-v2.pdf
-
Figure 8—source data 4
- https://cdn.elifesciences.org/articles/67141/elife-67141-fig8-data4-v2.pdf
To ask if DHCEO could physically interact with GR, we performed a molecular docking simulation between DHCEO and the ligand binding domain of GR. Molecular docking is an effective computational approach to understand protein-ligand interaction between small molecules and receptor proteins both energetically and geometrically (Ferreira et al., 2015). As seen in Figure 8B, DHCEO docks in the binding pocket of human GR favorably with a docking score of –9 (a negative value suggests favorable interactions), comparable to the docking score of OCDO at –9.5. Additional docking positions show that many of the predicted positions for OCDO can be recapitulated with DHCEO (Figure 8—figure supplement 1).
We further examined whether DHCEO could initiate GR and TrkB activation in Control hNPCs in vitro. Cultured hNPCs were exposed to DHCEO (3.5 μM), a physiologically relevant concentration, harvested at the different time points (3, 6, 10, and 24 hr), and evaluated for GR and TrkB activation by western blots (Figure 8C), which revealed that DHCEO-treated hNPCs showed a gradual increase of phosphorylated GR starting at 3 hr and peaking at 10 hr of exposure. As GR phosphorylation increased, DHCEO-treated hNPCs also showed the phosphorylation of TrkB from 6 hrs to 10 hr exposure, which dissipated at 24 hr exposure. As no exogenous GR ligands and neurotrophins were added in these experiments, these results indicated that DHCEO caused the increased phosphorylation of GR, which led to the activation of TrkB phosphorylation in human NPCs in vitro.
To confirm that GR activation could cause aberrant premature neurogenesis, we cultured human cortical precursors and transfected them with hDHCR7 shRNA-EGFP and control shRNA-EGFP. Twelve hr post-transfection, a glucocorticoid receptor antagonist, RU38486, or vehicle control was added and cultured for 3 days. Cultures were immunostained for EGFP and βIII-tubulin to evaluate the effects on neurogenesis. The analysis showed that inhibition of GR by RU38486 rescued the DHCR7-KD-mediated increase in neurogenesis down to the level observed in the control shRNA (Figure 8D).
These data suggest that premature cortical neurogenesis is promoted by GR activation by DHCEO and subsequent increased activation of the RTK-mediated MEK-ERK pathway. To directly evaluate this hypothesis, we inhibited MEK in cultured NPCs using two well-characterized MEK inhibitors, PD98059 and trametinib. The efficiency of these compounds in murine NPCs has been demonstrated previously (Tomita et al., 2020; Barnabé-Heider and Miller, 2003). Indeed, western blots demonstrated that both PD98059 and trametinib efficiently inhibited MEK activation in hNPCs (Figure 8M). Having confirmed the efficacy of these two compounds, we cultured SLOS hNPCs (SLOS 3044 and 5844) and wildtype hNPCs as well as murine Dhcr7-/- NPCs and wildtype NPCs, and treated them with either PD58059 or trametinib. The cultures were immunostained for βIII-tubulin 3 days later to evaluate the effect of the inhibitors on neurogenesis. As expected, both SLOS hNPCs and murine Dhcr7-/- NPCs increased the proportion of βIII+ newborn neurons, and the MEK inhibition by either PD98058 or trametinib reversed increased neurogenesis with DHCR7 mutations back to the level seen in the wildtype NPCs (Figure 8N, Figure 8—figure supplement 2C, F).
Chemical inhibitors may have off-target effects. Therefore, we performed rescue experiments by genetically knockdown either TrkB or MEK. We evaluated the efficiency of shRNA targeting TrkB and MEK by transfecting them independently into 293T cells and performed western blots (Figure 8O). These analyses showed that TrkB or MEK shRNA decreased TrkB or MEK protein whereas control shRNA did not. Either TrkB or MEK shRNA was transfected to cultured human neural progenitors with EGFP-DHCR7 shRNA. Quantification of EGFP+, βIII-tubulin + cells 3 days later demonstrated that coincident TrkB knockdown rescued the increased neurogenesis phenotype observed following shRNA-mediated DHCR7 knockdown (Figure 8K). Similarly, MEK knockdown also reversed the premature neurogenesis with DHCR7 shRNA knockdown in human NPCs (Figure 8L). Thus, DHCR7 mutations disrupt the normal cortical neurogenesis by hyperactivating RTKs, such as TrkB, through GR activation and thus activating the downstream MEK-ERK1/2 pathway.
Finally, we assessed whether aberrant premature neurogenesis was due to DHCEO, as it is a major oxysterol derived from free radical (non-enzymatic) oxidation of 7-DHC (Xu et al., 2011; Figure 2). Antioxidants can prevent or slow down the formation of oxysterols via the free radical mechanism and protect cells from the deleterious effects of oxysterols (Yin et al., 2011). In particular, vitamin E (VE), but not vitamin C (VC), has been shown to effectively inhibit the formation of 7-DHC-derived oxysterols in SLOS patient-derived fibroblasts (Korade et al., 2014). Combination with VC, which recycle oxidized VE, enhances the antioxidant activity of VE against lipid peroxidation (Doba et al., 1985). Furthermore, glutathione (GSH) is an important detoxifying molecule that is abundant (in mM) in cells and can potentially inhibit the formation of DHCEO by reacting with its electrophilic precursor, 7-DHC 5α,6α-epoxide (Porter et al., 2020; Xu et al., 2011). To test this possibility, we treated hDHCR7 KD human NPCs with antioxidants (VE, VC, VE/VC, or GSH) and then immunostained for EGFP and βIII-tubulin to evaluate the effects on neurogenesis (Figure 8E–F). The analysis indicated that VE, VE/VC, or GSH, but not VC alone, effectively reversed increased neurogenesis caused by hDHCR7 KD to the level observed in the control. Potential detoxifying effects of these antioxidants were further tested in human SLOS patient-derived NPCs as well as cortical precursors from Dhcr7-/- mouse embryos in vitro (Figure 8H–I and Figure 8—figure supplement 2A, B, D, E). Treatments with VE, VE/ VC and GSH were again found to effectively reduce the increased neurogenesis in both human and murine NPCs with Dhcr7 mutations down to the level observed in WT or Control samples. LC-MS/MS analysis confirmed that VE, VE/VC, or GSH indeed inhibited the levels of DHCEO in SLOS hiPSCs (Figure 8J). These results suggest that free radical chain-breaking antioxidants, such as VE, and nucleophilic antioxidants, such as GSH, can effectively rescue the neurogenesis phenotype observed in SLOS NPCs by inhibiting the free radical oxidation of 7-DHC at different stages (Figure 8—figure supplement 2G).
In addition to antioxidant treatment, we evaluated whether cholesterol plays significant roles in normal neurogenesis in human and murine NPCs. We tested whether cholesterol depletion could phenocopy the premature neurogenesis observed in both human and murine DHCR7 mutations. Human and murine NPCs were treated with of β-cyclodextrin, a cholesterol-depleting agent, and the changes in the proportion of βIII-tubulin+neurons were quantified 3 days after plating (Figure 8—figure supplement 3B). LC-MS/MS analysis demonstrated that β-cyclodextrin treatment reduced cellular cholesterol by more than 50% (Figure 8—figure supplement 3A); however, it did not change the proportion of βIII-tubulin+neurons and Ki67 + progenitors either in human NPCs or murine NPCs (Figure 8—figure supplement 3C, D). Furthermore, we tested whether restoration of cholesterol could potentially rescue aberrant premature neurogenesis observed in both human and murine SLOS NPCs (Figure 8H–I, Figure 8—figure supplement 2B, E). Low-density lipoprotein was added to cultures to examine the effects of cholesterol on neurogenesis. The results revealed that addition of cholesterol did not prevent increased neurogenesis in SLOS NPCs (Figure 8H–I, Figure 8—figure supplement 2B, E). Because DHCEO has been shown to inhibit Smo in the Hh signaling pathway (Sever et al., 2016), we also tested whether supplementation of SAG (a Smo agonist) can rescue the phenotype with or without cholesterol supplementation in DHCR7-KD hNPCs (Figure 8G). However, none rescued the neurogenesis phenotype. Thus, the lack of cholesterol and inhibition of Hh signaling do not contribute to the neurogenesis phenotype observed in human and murine NPCs with DHCR7 mutations.
Taken together, the abnormal premature neurogenesis in cortical precursor with Dhcr7 mutations was caused by DHCEO activation of GR and further activation of Trk-mediated neurogenic signaling pathway, but not by the deficiency in cholesterol, and inhibition of the formation of DHCEO or GR rescues the neurogenic defects.
Discussion
Whether deficiency in cholesterol, accumulation of 7-DHC, or accumulation of 7-DHC oxysterols is more important for the neurological and developmental phenotypes observed in SLOS patients remains unknown. Here, we characterized the neurogenic phenotype of SLOS cortical precursors in vitro and in vivo and showed that the accumulated 7-DHC-derived oxysterols, particularly DHCEO, disrupt normal embryonic neurogenesis by accelerating progressive depletion of cortical precursors through neurogenesis, ultimately resulting in greatly reduced cerebral cortex thickness with abnormal cortical layering.
The data presented here support four major conclusions. First, LC-MS/MS analyses indicate that DHCR7 mutations promote a significant accumulation of 7-DHC-derived oxysterols in murine and human embryonic cortical NPCs during cortical development. Second, our studies with KD or KO of DHCR7 indicate that DHCR7 is necessary for the normal proliferation of embryonic NPCs and genesis of newborn neurons in culture and within the environment of the embryonic cortex. KD or KO of DHCR7 leads to premature differentiation of cortical precursors to neurons and ultimately a reduction in the thickness of cortical layers. This occurs through the activation of the neurogenic TrkB-MEK-C/EBP pathway. Third, we show that 7-DHC-derived oxysterols, especially DHCEO, display detrimental effects on normal neural development, and that DHCEO can activate GR, which is responsible for abnormal cortical precursor differentiation, since DHCR7 KD/KO phenotype can be rescued by concurrent inhibition of glucocorticoid receptor. Fourth, we show that premature differentiation of cortical precursors with DHCR7 mutations is cholesterol-independent since inhibition of 7-DHC-derived oxysterols via antioxidant treatment can rescue DHCR7 KD/KO phenotype, whereas cholesterol supplementation cannot.
Based on these data, we propose a model where DHCEO binds and activates GR, and in doing so, controls the RTK-mediated neurogenic pathway, TrkB-MEK-C/EBP, and thus fate-decision of developing precursors and neurons. Although docking studies only approximate enthalpic energies and are an incomplete approximation of affinity, they provide a structural rationale for this activity. This model is consistent with previous studies showing that an oxysterol structurally similar to DHCEO, OCDO, can bind and regulate GR and its transcriptional activity (Voisin et al., 2017) and ligand-bound GR promoted TrkB phosphorylation, leading to activation of its downstream effectors, MEK and ERK (Jeanneteau et al., 2008). Our model of DHCEO exerting its effect on neurogenesis through GR does not contradict the previous finding by Francis et al., which suggests that Wnt/β-catenin inhibition contributes to precocious neurogenesis (Francis et al., 2016), because activation of GR has been shown to inhibit Wnt/β-catenin signaling in several systems (Olkku and Mahonen, 2009; Zhou et al., 2020).
This work represents the first comprehensive characterization of the neurogenic phenotype in the SLOS mouse model and SLOS patient-derived NPCs. We demonstrate that the aberrant neural development observed in mice and human NPCs with DHCR7 mutations occurs in a cholesterol-independent and 7-DHC-derived oxysterol-dependent manner. Successful rescue of the neurogenic phenotype by inhibiting GR or inhibiting the formation of oxysterols with antioxidants pave the wave for potential therapies for the neurological defects observed in SLOS patients.
Materials and methods
Animals
All animal experiments were performed in accordance with the Guideline ‘Guide for the Care and Use of Laboratory Animals’ of the National Institutes of Health and were approved by the University of Washington Institutional Animal Care and Use Committee. Dhcr7 mice (B6.129P2(Cg)-Dhcr7tm1Gst/J) were maintained as heterozygous and were genotyped as described previously (Fitzky et al., 2001). The primers used are the following: Dhcr7-WT-F: 5’-GGATCTTCTGAGGGCAGCTT-3’; Dhcr7-WT-R: 5’-TCTGAACCCTTGGCTGATC-3’; Delta-Mut: 5’-CTAGACCGCGGCTAGAGAAT-3’. For in vitro transfection, C57BL/6 J E12.5 pregnant mice were obtained by time-mating. Mating pairs of wild-type (Dhcr7+/+) C57BL/6 J mice were purchased from Jackson Laboratories (Bar Harbor, ME). All mice had free access to rodent chow and water in a 12 hr dark-light cycle room.
Cells
Primary human fibroblasts isolated from SLOS patients (GM03044 and GM05788) were purchased from Coriell Institute. These lines have been sequenced by Coriell Institute to confirm their mutations that affect the function of DHCR7, and the biochemical defects have been confirmed by measuring the 7-dehydrocholesterol/cholesterol ratio. The cultures were also tested to be free of mycoplasma, bacteria, and fungi contamination by the vendor. Human embryonic kidney 293T cells were purchased from ATCC (catalog #: CRL-3216), which carried out authentication using STR profiling and tested to be mycoplasma free.
Plasmids
The target sequence for murine Dhcr7 shRNA was cloned into pLKO-UBI-GFP digested with EcoRI and PacI. pLKO-UBI-GFP was generated by digesting out hPGK promoter from pLKO.3G and ligating ubiquitin promoter (UBI) from pCLX-UBI-VenusN with PacI and BamHI. pLKO.3G was a gift from Christophe Benoist & Diane Mathis (Addgene plasmid # 14748; http://n2t.net/addgene:14748; RRID:Addgene_14748) and pCLX-UBI-VenusN was a gift from Patrick Salmon (Addgene plasmid # 27247; http://n2t.net/addgene:27247; RRID:Addgene_27247). cDNAs encoding murine (pCMV-mDHCR7-Myc/DDK) and human DHCR7 (pCMV-hDHCR7-Myc/DDK) were purchased from Origene (MR223420 and RC228922 for murine and human cDNA clone respectively). PB-EF1α-GreenPuro-H1MCS and Super PiggyBac transposase expression vector from System Biosciences (Cat#PBS506A-1 and Cat# PB210PA-1 respectively) were used for clonal analysis.
Cortical precursor cell cultures
Request a detailed protocolMurine cortical precursor cells were cultured as described previously (Barnabé-Heider and Miller, 2003; Tomita et al., 2020). Briefly, mouse cortical precursor cells from cortices were dissected from E12.5 Dhcr7+/+ or Dhcr7-/- (KO) mouse embryos in ice-cold HBSS (Invitrogen) and transferred into cortical precursor medium. The cortical precursor medium consisted of Neurobasal medium (Invitrogen) with 500 μM L-glutamine (Invitrogen), 2% B27 supplement (Invitrogen), 1% penicillin-streptomycin (Invitrogen) and 40 ng/ml FGF2 (BD Biosciences). The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich). Plating density of the cortical precursors was 150,000 cells/well in 24-well plates for single embryo cultures and plasmid transfections.
Human induced pluripotent stem cells (hiPSCs)
Request a detailed protocolHuman fibroblasts (GM03044 and GM05788; see above) isolated from patients with SLOS were reprogrammed as described elsewhere (Okita et al., 2011; Yu et al., 2007). Briefly, 1x106 cells of human fibroblast cells at early passages were trypsinized (0.25% Trypsin/0.5 mM EDTA, Gibco) and electroporated with indicated episomal plasmids using Amaxa Basic Nucleofector Kit for primary mammalian fibroblasts, program A-24 (Lonza). 1.0 μg pSIN4-EF2-O2S and pSIN4-EF2-N2L were used for each electroporation. The electroporated human fibroblasts were seeded onto a gelatin-coated tissue culture dish and cultured with fibroblast medium (DMEM with 10% FBS, 2 mM L-glutamine, 1% non-essential amino acids, 1% sodium pyruvate, and 1% penicillin and streptomycin) for 7 days and re-seeded onto feeder layer cells (irradiated SNL 76/7 feeder cells; a gift from Dr. Allan Bradley, Sanger Institute, UK) with hiPSC medium (DMEM/F12 medium with 20% knockout serum replacer, 1% sodium pyruvate, 1% non-essential amino acids, 0.007% 2-mercaptoethanol,1% penicillin and streptomycin, 0.1 mM sodium butyrate, 50 nM suberoylanilide hydroxamic acid, and 4 ng/ml of bFGF) in 100 mm tissue culture dishes and continued to be cultured until hiPSC colonies were visible (28 days from electroporation). The hiPSC colonies were then picked and further cultured for expansion.
Maintenance and differentiation of hiPSC
Request a detailed protocolUndifferentiated hiPSCs were cultured and maintained in mTeSR Plus medium (StemCell Technologies) on Matrigel (BD Biosciences)-coated plates prior to the generation of human neural progenitor cells (hNPCs). hNPCs were generated using the STEMdiff SMADi Neural Induction kit (StemCell Technologies) according to the manufacturer’s protocol. Briefly, neuralized embryoid bodies (EBs) were generated by culturing small aggregates of hESCs in ultra-low attachment plates (Corning) in STEMdiff SMADi Neural induction medium. EBs were replated and cultured in poly-D-lysine/Laminin-coated plates with the neural induction medium until EBs formed neural rosette formations. Neural rosettes were collected and replated in poly-D-lysine/Laminin-coated plates for hNPCs outgrowth in neural induction medium until hNPCs were ready for the first passage. hNPCs were maintained in STEMdiff Neural Progenitor medium. For neurogenesis of hNPCs, hNPCs were cultured in Neurobasal A medium supplemented with B27 minus vitamin A, 1% penicillin-streptomycin and glutamax (all from Invitrogen) in poly-D-lysine/Laminin-coated plates (Konopka et al., 2012; Usui et al., 2017). For quantification, an average of 700 cells was counted per condition in 5–6 random fields per independent experiment.
In vitro differentiation of iPSCs
Request a detailed protocolFor in vitro differentiation of human iPSCs to the three germ layers, STEMdiff Trilineage Differentiation Kit (StemCell Technologies) was used as described by the manufacturer. Briefly, cells were harvested by Gentle Cell Dissociation Reagent (StemCell Technologies) and plated onto Matrigel-coated 24-well plates with mTeSR1 medium (StemCell Technologies). Cell density and viability were determined using trypan blue exclusion. Cells were seeded at a clonal density of 200,000 cells/cm2 for ectoderm and endoderm differentiation and 50,000 cells/cm2 for mesoderm differentiation. Twenty-four hr after plating, cells were switched to the appropriate STEMdiff Trilineage medium for ectoderm, mesoderm and endoderm differentiation. Cells were cultured in the lineage-specific medium for 5 days (mesoderm and endoderm lineages) and 7 days (ectoderm lineage) and were harvested and/or fixed for analyses of lineage-specific markers for the three germ layers (Figure 1—figure supplement 1).
Transfection and quantification
Request a detailed protocolFor plasmid transfection of mouse cortical precursors, Lipofectamine LTX and Plus Reagent (Invitrogen) were used as described by the manufacturer. Briefly, 1 μg of DNA and 1 μl of Lipofectamine LTX and Plus Regent in 100 μl of Opti-MEM (both from Invitrogen) were mixed, incubated for 20 min, and added to precursors three hours after plating. For clonal analysis, 1.5 μg DNA, at 1:3 ratio of Super PiggyBac transposase expression vector to shRNA or control plasmids, were incubated as described above and added to cortical precursors three hours after plating. For plasmid transfection of human cortical precursors, Lipofectamine Stem Transfection Reagent (Invitrogen) was used as described by the manufacturer. Briefly, 1 μg of DNA and 1 μl of Lipofectamine Stem Transfection Reagent were prepared separately in 25 μl of Opti-MEM medium (Invitrogen), mixed, incubated for 30 min and added to precursors one day after plating. The target sequences for murine and human Dhcr7 shRNA were 5’- GGAAGGTGCTTCTTGTTTA-3’ and 5’- GGAAGTGGTTTGACTTCAA-3’ respectively. The target sequence for the control shRNA was 5’-TCCCAACTGTCACGTTCTC-3’. For quantification, immunostaining and image acquisition were performed, and >100 cells per condition per experiment were counted and analyzed, and experiments were performed with 3 embryos per plasmid transfected and analyzed individually. For inhibition of MEK/ERK, PD98059 at 50 μM or Trametinib at 100 nM were added to precursors 24 hours after plating or transfection, and cultures were analyzed 3 days later. For depletion of cholesterol, β-cyclodextrin (at 0.5 mM, 2.5 mM and 5 mM) was added to the precursors for 30 min at 37 °C, washed with PBS, cultured with neural precursor medium and analyzed 3 days later. Quantification, immunostaining, and image acquisition were performed and >100 cells per condition per experiment were counted and analyzed, and experiments were performed with three embryos per genotype or per plasmid transfected individually.
Neurosphere cultures
Request a detailed protocolE13.5 cortices from Dhcr7+/+ or Dhcr7-/- embryos were dissected and mechanically dissociated into a single-cell suspension by a fire-polished glass pipette as previously described (Capecchi and Pozner, 2015; Tomita et al., 2020). Cell density and viability were determined using trypan blue exclusion. Cells were seeded in triplicate at a clonal density of 10 cells/μl in 6 well (2 ml/well) ultra-low attachment culture plates (Coster) in serum-free medium supplemented with 20 ng/ml EFG (Sigma), 20 ng/ml FGF2 (Sigma), 2% B27 supplement (Invitrogen) and 2 μg/ml heparin (Sigma). Neurospheres were cultured for 6 days at 37 °C. To evaluate self-renewal potential, neurospheres were mechanically dissociated into single cell suspensions by fire-polished glass pipette, passed through a 45 μm nylon screen cell strainer, and cultured at a clonal density of 2 cells/μl for an additional 6 days.
Immunocytochemistry and histological analysis
Request a detailed protocolFor morphometric analysis, immunostaining of tissue sections was performed as described (Capecchi and Pozner, 2015). Briefly, brain sections were permeabilized and blocked in PBST (1 X PBS, 0.5% (v/v) Triton X-100) containing 10% NGS for 1 hr. Brain slices were incubated with primary antibodies in PBST with 5% NGS at 4 °C overnight. The sections were incubated with secondary antibodies in PBST with 5% NGS for 1–2 hr at room temperature. Sections were counterstained with DAPI (Thermo Fisher Scientific). Slides were mounted in Fluoromount-G anti-fade reagent (Southern Biotech). Digital image acquisition was performed with EVO-FL Imagining System (Thermo Fisher Scientific). For quantification of precursor and neuron numbers, we analyzed sections at the medial-lateral level, counting all marker-positive cells in a 200 µm wide strip of the cortex, extending from the meninges to the ventricle. In all cases, we analyzed at least 3 similar cortical sections/embryo or pup from three different embryos or pups per genotype (for a total of at least 9 sections per genotype).
Antibodies
The primary antibodies used for immunostaining were chicken anti-GFP (1:1000; Abcam), rabbit anti-Dhcr7 (1:100; Thermo Fisher Scientific), rabbit anti-Sox2 (1:200; Cell Signaling Technology), rabbit anti-Pax6 (1:1000; Covance), mouse anti-Ki67 (1:200; BD Biosciences), mouse anti-βIII-tubulin (1:1000; Covance), rabbit anti-βIII-tubulin (1:1000; Covance), rabbit anti-Tbr1 (1:500; Abcam), mouse anti-Satb2 (1:400; Abcam), rat anti-Ctip2 (1:500; Abcam), and rabbit anti-cleaved caspase 3 (1:400; Cell Signaling Technology), mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200; R&D Systems), goat anti-NCAM (1:200; R&D Systems), goat anti-Sox17 (1:200; R&D Systems), rabbit anti-Foxa2 (1:1000; Abcam). The secondary antibodies used for immunostaining were Rhodamine (TRITC)-conjugated goat anti-mouse and anti-rabbit IgG (1:500; Jackson ImmunoResearch Laboratories) and Alexa Fluor 488-conjugated goat anti-mouse, anti-rat and anti-rabbit IgG (1:800; Jackson ImmunoResearch Laboratories), Alexa Flour 488-conjugated donkey anti-goat IgG (1:800; Jackson ImmunoResearch Laboratories), Rhodamine (TRITC)-conjugated donkey anti-goat (1:200; Jaclson ImmunoResearch Laboratories). The primary antibodies used for immunoblotting were rabbit anti-Dhcr7 (1:1000; Abcam), rabbit anti-GAPDH (1:5000; Santa Cruz Biotechnology), rabbit anti Erk1/2 (1:5000; Santa Cruz Biotechnology), rabbit anti-TrkB (1:1000; Cell Signaling Technology), rabbit anti-phospho-TrkB (1:1000; Cell Signaling Technology), rabbit anti-glucocorticoid receptor (1:1000; Cell Signaling Technology), rabbit anti-phospho-glucocorticoid receptor (1:1000; Cell Signaling Technology), rabbit anti-MEK (1:1000; Cell Signaling Technology), rabbit anti-phospho-MEK (1:1000; Cell Signaling), rabbit anti-phospho-cebpβ (1:1000; Cell signaling Technology) and rabbit anti-cebpβ (1:1000; Cell Signaling Technology), rabbit anti-Pax6 (1:1000; Covance), mouse anti-Nestin (1:1000; StemCell Technologies), rabbit anti-Brachyury (1:200; R&D Systems), rabbit anti-Tbr2 (1:500; Abcam), goat anti-Sox17 (1:200; R&D Systems), rabbit anti-Foxa2 (1:1000; Abcam). The secondary antibodies used for immunoblotting were HRP-conjugated goat anti-mouse IgG (1:5000; Jackson ImmunoResearch Laboratories) and anti-rabbit IgG (1:10,000; Jackson ImmunoResearch Laboratories), HRP-conjugated donkey anti-goat IgG (1:1000; Jackson ImmunoResearch Laboratories).
RT-PCR
Request a detailed protocolTotal RNA was isolated with Trizol and cDNA was prepared using the SuperScript III Reverse Transcriptase kit (Invitrogen) according to the manufacturer’s protocols. Primer sequences are the following: Dhcr7-F: 5’-TATGAGGTGAATGGGCTGCA-3’; Dhcr7-R: 5’-GGTTAATGAGGGTCCAGGCT –3’; β-actin-F: 5’-GATGACGATATCGCTGCGCTG-3’; β-actin-R: 5’- GTACGACCAGAGGCATACAGG-3’. All PCR products were single bands with predicted molecular weights and confirmed by DNA sequencing.
Quantitative PCR
Request a detailed protocolTotal RNA was extracted with Tri-Reagent (Sigma) treated with DNAse I (Fermentas, Thermo Scientific, Waltham, MA, USA) and cDNA was synthesized from 1 μg of RNA using the SuperScript IV Reverse Transcriptase Kit (Invitrogen) according to the manufacturer’s protocols. Quantitative PCR was performed using Taqman Fast Advance Master Mix (Thermo Fisher Scientific) and Taqman probes targeted against either Dhcr7 (Mm01164321_m1) or β-Actin (Mm00607939_s1). β-Actin mRNA was used as an endogenous control for all reactions, and all reactions were performed in triplicate. Quantitative PCR was performed and analyzed using StepOne Plus Real-Time PCR system (ThermoFisher Scientific).
Western blotting
Request a detailed protocolEmbryonic cortices or neurosphere cultures were lysed in RIPA buffer (50 mM Tri pH8, 150 mM NaCl, 1% NP-40, 0.1% SDS, 1 mM EDTA) containing 1 mM PMSF (phenylmethanesulfonyl fluoride), 1 mM sodium vanadate, 20 mM sodium fluoride 10 μg/ml aprotinin and 10 μg/ml leupeptin. A total of 10–20 μg of protein lysate was electrophoresed, and western blots were performed as described previously (Barnabé-Heider and Miller, 2003).
RNA sequencing and data analysis
Request a detailed protocolRaw RNA sequencing reads in FASTQ format were mapped to the human genome using HISAT (https://ccb.jhu.edu/software/hisat/; Last accessed June 19, 2022), and format conversions were performed using Samtools. Cufflinks (http://cole-trapnell-lab.github.io/cufflinks/; Last accessed June 19, 2022) was used to estimate the relative abundances of transcripts from each RNA sample. Cuffdiff, a module of Cufflinks, was then used to determine differentially expressed genes (DEGs) between Control and DHCR7-KO hNPCs. DEGs met the following criteria: adjusted <i>P-value < .05 (corresponding to the allowed false discovery rate of 5%) and fold-change >1.5 between genotypes. A two-way hierarchical clustering dendrogram (complete-linkage method, Euclidean distance scale) of DEGs was used to visualize biological variability among samples, generated by R software using the ‘pheatmap’ package (https://cran.r-project.org/web/packages/pheatmap/). To elucidate the biological functions of DEGs, the Core Analysis feature of Ingenuity Pathway Analysis (IPA, Qiagen) was used to identify significantly enriched Diseases and Biological Functions related to the nervous system. Network interactions among DEGs involved in the Biological Function ‘development of the central nervous system’ were assessed using STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) analysis, set at the highest confidence interaction score and only connected nodes displayed (https://string-db.org/cgi/input.pl?sessionId=xCahIfrzvltC; Last accessed June 19, 2022). Enriched KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were identified among DEGs in the STRING network. Finally, a Venn diagram was generated to demonstrate the overlap between genes dysregulated in KO hNPCs and genes in the SFARI database, a collection of genes implicated in autism susceptibility (https://gene.sfari.org/; Last accessed June 19, 2022). Raw data of the RNA sequencing has been deposited at Dryad (https://doi.org/10.5061/dryad.zw3r2287f). The list of differentially expressed genes can be found in Supplementary file 2 (Excel).
Oxysterol and sterol analysis
Request a detailed protocolCell pellets were resuspended in 300 μL of 1 X PBS and lysed by sonication in an ice bath for 30 min. Protein determination was performed using the BioRad DC protein mass assay (BioRad, Hercules, CA). Internal standard mixtures for sterols and oxysterols analysis were added to each sample (see Supplementary files 1–4 for a list of standards and their concentrations used). Lipid extraction was performed using the Folch method as described previously (Fliesler et al., 2018; Folch et al., 1957; Herron et al., 2018). The dried lipid extract was reconstituted with 200 μL of methylene chloride and stored at –80 °C until analysis. Prior to analysis, 50 μL of extract was transferred to into glass LC vials, dried under argon, and reconstituted with 50 μL of 90% methanol in water with 0.1% formic acid. For tissue samples, sterol and oxysterol internal standard mixtures were added to whole tissues, which were subsequently homogenized in 4 mL Folch solution with 1 mL 0.9% NaCl. The dried lipid extract was reconstituted with 0.5 mL (for tissues < 50 mg), 1.0 mL (tissues > 50 < 100 mg) or 1.5 mL (>100 mg) of methylene chloride. Prior to analysis, 50 μL of lipid extract (30 μL for tissues > 100 mg) was transferred into glass LC vials, dried under argon, and reconstituted with 50 μL 90% methanol in water with 0.1% formic acid. Determination of oxysterol and sterol concentrations in cells and tissues was performed by ultra-performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) on a SCIEX 6500 triple quadrupole mass spectrometer (for oxysterols) or a SCIEX 4000 QTRAP (for sterols) mass spectrometer with atmospheric pressure chemical ionization (APCI) coupled to a Waters Acquity UPLC system, as described previously (Fliesler et al., 2018; Herron et al., 2018). Briefly, sterols and oxysterols were separated by reverse-phase chromatography on a C18 column (1.7 mm, 2.1x100 mm, Phenomenex Kinetex) using a 15 min isocratic gradient of 90% methanol with 0.1% formic acid at a flow of 0.4 mL/min. Selective reaction monitoring (SRM) was used to monitor the dehydration of the sterol and oxysterol [M+H]+ ions to generate [M+H-H2O]+ ions (See Supplementary files 1-4 for a list of retention times and MS/MS transitions for the standards used). The APCI parameters were as follows: nebulizer current, 3 mA; temperature, 350 °C; curtain gas, 20 psi; ion source gas, 55 psi. The MS conditions for SRM analysis were as follows: declustering potential, 80 V; entrance potential, 10 V; collision energy, 25 V; collision cell exit potential, 20 V. Data analysis was performed with Analyst (v. 1.6.2) Quantitation Wizard. Analyte concentrations in cells and tissues were determined relative to the internal standard levels and the relative response factor (RRF) of each analyte was calculated from a mixture of sterol and oxysterol standards and internal standards. Concentrations were normalized to cell protein weight or tissue weight.
Docking simulation
Request a detailed protocolDocking simulations were performed with Autodock Vina 1.1.2. The model ligands and 3D models were built and generated by Openbabel 2.3.2. The crystal structure of the glucococorticoid receptor used for docking simulations was crystal structure 4p6w available from Protein Data Bank (http://www.rcsb.org/structure/4p6w). The mometasone furoate molecule present in that crystal structure was used to establish the center of the search area defining the binding pocket, and ligand docking calculations were performed within that search area.
Statistical analysis
Request a detailed protocolIn general, three biological replicates were used for each experiment based on the strong phenotype observed in the Dhcr7-/- SLOS model and our previous publication showing the large differences in oxysterols and sterol levels in WT and KO brains at birth (Xu et al., 2011). For culturing of mouse cortical precursors or neurospheres from single embryos, one litter is considered a biological replicate. For hNPC culture, each separate preparation of hNPC from hiPSC is considered a biological replicate. For in vivo study, one litter is considered a biological replicate. Statistical analyses were performed using two-tailed Student’s t-test assuming unequal variance when comparing two different groups unless otherwise indicated in the text. For immunostaining of cell culture or tissue sections, at least three technical replicates per biological replicate were performed. To analyze the multi-group neuroanatomical studies, we used one-way ANOVA unless otherwise indicated in the text. Significant interactions or main effects were further analyzed using Newman-Keuls post-hoc tests. All statistical tests were performed using Microsoft Excel or Prism 8 (GraphPad). In all cases, error bars indicate the standard error of the mean.
Data availability
Raw RNA sequencing data has been deposited at Dryad at https://doi.org/10.5061/dryad.zw3r2287f. This data was used to generate Figure 7 and Table S5.
-
Dryad Digital Repository7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome.https://doi.org/10.5061/dryad.zw3r2287f
References
-
Smith-Lemli-Opitz syndrome and autism spectrum disorderThe American Journal of Psychiatry 164:1655–1661.https://doi.org/10.1176/appi.ajp.2007.07020315
-
Aspm regulates symmetric stem cell division by tuning cyclin E ubiquitinationNature Communications 6:8763.https://doi.org/10.1038/ncomms9763
-
Antioxidant and co-antioxidant activity of vitamin C. the effect of vitamin C, either alone or in the presence of vitamin E or a water-soluble vitamin E analogue, upon the peroxidation of aqueous multilamellar phospholipid liposomesBiochimica et Biophysica Acta 835:298–303.https://doi.org/10.1016/0005-2760(85)90285-1
-
27-Hydroxycholesterol is an endogenous selective estrogen receptor modulatorMolecular Endocrinology 22:65–77.https://doi.org/10.1210/me.2007-0383
-
7-dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of smith-lemli-opitz/RSH syndromeThe Journal of Clinical Investigation 108:905–915.https://doi.org/10.1172/JCI12103
-
A simple method for the isolation and purification of total lipides from animal tissuesThe Journal of Biological Chemistry 226:497–509.https://doi.org/10.1016/S0021-9258(18)64849-5
-
Activation of Rho GTPases in Smith-Lemli-Opitz syndrome: pathophysiological and clinical implicationsHuman Molecular Genetics 19:1347–1357.https://doi.org/10.1093/hmg/ddq011
-
Modeling the functional genomics of autism using human neuronsMolecular Psychiatry 17:202–214.https://doi.org/10.1038/mp.2011.60
-
Biological activities of 7-dehydrocholesterol-derived oxysterols: implications for Smith-Lemli-Opitz syndromeJournal of Lipid Research 51:3259–3269.https://doi.org/10.1194/jlr.M009365
-
Neuronal subtype specification in the cerebral cortexNature Reviews. Neuroscience 8:427–437.https://doi.org/10.1038/nrn2151
-
Malformation syndromes caused by disorders of cholesterol synthesisJournal of Lipid Research 52:6–34.https://doi.org/10.1194/jlr.R009548
-
Cilia-Associated oxysterols activate smoothenedMolecular Cell 72:316–327.https://doi.org/10.1016/j.molcel.2018.08.034
-
The near universal presence of autism spectrum disorders in children with smith–lemli–opitz syndromeAmerican Journal of Medical Genetics Part A 140A:1511–1518.https://doi.org/10.1002/ajmg.a.31294
-
Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesisNature Chemical Biology 9:126–133.https://doi.org/10.1038/nchembio.1156
-
Development, behavior, and biomarker characterization of Smith-Lemli-Opitz syndrome: an updateJournal of Neurodevelopmental Disorders 8:12.https://doi.org/10.1186/s11689-016-9145-x
-
Abnormalities of cholesterol metabolism in autism spectrum disordersAmerican Journal of Medical Genetics. Part B, Neuropsychiatric Genetics 141B:666–668.https://doi.org/10.1002/ajmg.b.30368
-
Defective cholesterol biosynthesis associated with the Smith-Lemli-Opitz syndromeThe New England Journal of Medicine 330:107–113.https://doi.org/10.1056/NEJM199401133300205
-
Markedly increased tissue concentrations of 7-dehydrocholesterol combined with low levels of cholesterol are characteristic of the Smith-Lemli-Opitz syndromeJournal of Lipid Research 36:89–95.
-
Foxp1 regulation of neonatal vocalizations via cortical developmentGenes & Development 31:2039–2055.https://doi.org/10.1101/gad.305037.117
-
Mutations in the human sterol delta7-reductase gene at 11q12-13 cause Smith-Lemli-Opitz syndromeAmerican Journal of Human Genetics 63:55–62.https://doi.org/10.1086/301936
-
Rate constants for peroxidation of polyunsaturated fatty acids and sterols in solution and in liposomesJournal of the American Chemical Society 131:13037–13044.https://doi.org/10.1021/ja9029076
-
Oxysterols from free radical chain oxidation of 7-dehydrocholesterol: product and mechanistic studiesJournal of the American Chemical Society 132:2222–2232.https://doi.org/10.1021/ja9080265
-
An oxysterol biomarker for 7-dehydrocholesterol oxidation in cell/mouse models for Smith-Lemli-Opitz syndromeJournal of Lipid Research 52:1222–1233.https://doi.org/10.1194/jlr.M014498
-
Metabolism of oxysterols derived from nonenzymatic oxidation of 7-dehydrocholesterol in cellsJournal of Lipid Research 54:1135–1143.https://doi.org/10.1194/jlr.M035733
-
Free radical lipid peroxidation: mechanisms and analysisChemical Reviews 111:5944–5972.https://doi.org/10.1021/cr200084z
Article and author information
Author details
Funding
National Institutes of Health (R01 HD092659)
- Libin Xu
National Institutes of Health (T32 ES007032)
- Josi M Herron
National Institutes of Health (T32 GM007750)
- Amy Li
National Institutes of Health (TL1 TR002318)
- Amy Li
Smith-Lemli-Opitz/RSH Foundation (Research grant)
- Libin Xu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
This work was supported by a National Institutes of Health (NIH) grant R01HD092659 (LX) and a grant from the Smith-Lemli-Opitz/RSH Foundation. JMH was supported by the University of Washington (UW) Environmental Pathology/ Toxicology Training Program (NIH T32 ES007032) and AL was supported by the UW Pharmacological Sciences Training Program (NIH T32 GM007750) and the Institute of Translational Health Sciences TL1 Program (NIH TL1 TR002318). We also thank Dr. Carol Ware and Christopher Cavanaugh for thier technical advice on hiPSC culture.
Ethics
This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (#4350-01) of the University of Washington.
Copyright
© 2022, Tomita et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,184
- views
-
- 216
- downloads
-
- 12
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Neuroscience
Combining electrophysiological, anatomical and functional brain maps reveals networks of beta neural activity that align with dopamine uptake.
-
- Neuroscience
During rest and sleep, memory traces replay in the brain. The dialogue between brain regions during replay is thought to stabilize labile memory traces for long-term storage. However, because replay is an internally-driven, spontaneous phenomenon, it does not have a ground truth - an external reference that can validate whether a memory has truly been replayed. Instead, replay detection is based on the similarity between the sequential neural activity comprising the replay event and the corresponding template of neural activity generated during active locomotion. If the statistical likelihood of observing such a match by chance is sufficiently low, the candidate replay event is inferred to be replaying that specific memory. However, without the ability to evaluate whether replay detection methods are successfully detecting true events and correctly rejecting non-events, the evaluation and comparison of different replay methods is challenging. To circumvent this problem, we present a new framework for evaluating replay, tested using hippocampal neural recordings from rats exploring two novel linear tracks. Using this two-track paradigm, our framework selects replay events based on their temporal fidelity (sequence-based detection), and evaluates the detection performance using each event's track discriminability, where sequenceless decoding across both tracks is used to quantify whether the track replaying is also the most likely track being reactivated.