1. Cell Biology
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Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth

  1. Lara Katharina Krüger  Is a corresponding author
  2. Matthieu Gélin
  3. Liang Ji
  4. Carlos Kikuti
  5. Anne Houdusse
  6. Manuel Théry
  7. Laurent Blanchoin
  8. Phong T Tran  Is a corresponding author
  1. Institut Curie, France
  2. Institut de Recherche Saint Louis, France
  3. Institut Curie, Centre National de la Recherche Scientifique, France
  4. CEA - Hopital Saint Louis, France
  5. Interdisciplinary Research Institute Grenoble, France
Research Article
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Cite this article as: eLife 2021;10:e67489 doi: 10.7554/eLife.67489

Abstract

Mitotic spindle function depends on the precise regulation of microtubule dynamics and microtubule sliding. Throughout mitosis, both processes have to be orchestrated to establish and maintain spindle stability. We show that during anaphase B spindle elongation in S. pombe, the sliding motor Klp9 (kinesin-6) also promotes microtubule growth in vivo. In vitro, Klp9 can enhance and dampen microtubule growth, depending on the tubulin concentration. This indicates that the motor is able to promote and block tubulin subunit incorporation into the microtubule lattice in order to set a well-defined microtubule growth velocity. Moreover, Klp9 recruitment to spindle microtubules is dependent on its dephosphorylation mediated by XMAP215/Dis1, a microtubule polymerase, creating a link between the regulation of spindle length and spindle elongation velocity. Collectively, we unravel the mechanism of anaphase B, from Klp9 recruitment to the motors dual-function in regulating microtubule sliding and microtubule growth, allowing an inherent coordination of both processes.

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A source data file for all data sets used in Figure 1- 7 has been provided.

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Author details

  1. Lara Katharina Krüger

    UMR144, Institut Curie, Paris, France
    For correspondence
    lara-katharina.kruger@curie.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0439-951X
  2. Matthieu Gélin

    Human Immunology Pathophysiology Immunotherapy, Institut de Recherche Saint Louis, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Liang Ji

    UMR144, Institut Curie, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Carlos Kikuti

    UMR144, Institut Curie, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Anne Houdusse

    Structural Motility, Institut Curie, Centre National de la Recherche Scientifique, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8566-0336
  6. Manuel Théry

    CEA - Hopital Saint Louis, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  7. Laurent Blanchoin

    Laboratoire de Physiologie Cellulaire & Végétale, Interdisciplinary Research Institute Grenoble, Grenoble, France
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8146-9254
  8. Phong T Tran

    UMR144, Institut Curie, Paris, France
    For correspondence
    phong.tran@curie.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2410-2277

Funding

French Ministry of Science and Education

  • Lara Katharina Krüger

Fondation ARC pour la Recherche sur le Cancer

  • Lara Katharina Krüger

La Ligue Contre le Cancer

  • Phong T Tran

Fondation ARC pour la Recherche sur le Cancer

  • Phong T Tran

INCa

  • Phong T Tran

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Thomas Surrey, Centre for Genomic Regulation (CRG), Spain

Publication history

  1. Received: February 12, 2021
  2. Accepted: June 2, 2021
  3. Accepted Manuscript published: June 3, 2021 (version 1)

Copyright

© 2021, Krüger et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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