1. Plant Biology
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CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1

  1. Andreas Kolbeck
  2. Peter Marhavý
  3. Damien De Bellis
  4. Baohai Li
  5. Takehiro Kamiya
  6. Toru Fujiwara
  7. Lothar Kalmbach
  8. Niko Geldner  Is a corresponding author
  1. University of Lausanne, Switzerland
  2. University of Tokyo, Japan
Research Article
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Cite this article as: eLife 2022;11:e69602 doi: 10.7554/eLife.69602

Abstract

Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Andreas Kolbeck

    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  2. Peter Marhavý

    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  3. Damien De Bellis

    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  4. Baohai Li

    Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan
    Competing interests
    The authors declare that no competing interests exist.
  5. Takehiro Kamiya

    Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan
    Competing interests
    The authors declare that no competing interests exist.
  6. Toru Fujiwara

    Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5363-6040
  7. Lothar Kalmbach

    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  8. Niko Geldner

    Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
    For correspondence
    niko.geldner@unil.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2300-9644

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (31003A_156261)

  • Niko Geldner

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (310030B_176399)

  • Niko Geldner

Federation of European Biochemical Societies

  • Peter Marhavý

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Yoselin Benitez-Alfonso, University of Leeds, United Kingdom

Publication history

  1. Received: April 20, 2021
  2. Accepted: January 13, 2022
  3. Accepted Manuscript published: January 14, 2022 (version 1)

Copyright

© 2022, Kolbeck et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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