Structural insight into the dual function of LbpB in mediating Neisserial pathogenesis
Abstract
Lactoferrin-binding protein B (LbpB) is a lipoprotein present on the surface of Neisseria that has been postulated to serve dual functions during pathogenesis in both iron acquisition from lactoferrin (Lf), and in providing protection against the cationic antimicrobial peptide lactoferricin (Lfcn). While previous studies support a dual role for LbpB, exactly how these ligands interact with LbpB has remained unknown. Here, we present the structures of LbpB from N. meningitidis and N. gonorrhoeae in complex with human holo-Lf, forming a 1:1 complex and confirmed by size-exclusion chromatography small-angle X-ray scattering. LbpB consists of N- and C-lobes with the N-lobe interacting extensively with the C-lobe of Lf. Our structures provide insight into LbpB’s preference towards holo-Lf, and our mutagenesis and binding studies show that Lf and Lfcn bind independently. Our studies provide the molecular details for how LbpB serves to capture and preserve Lf in an iron-bound state for delivery to the membrane transporter LbpA for iron piracy, and as an antimicrobial peptide sink to evade host immune defenses.
Introduction
There are 17 species of Neisseria that colonize humans with only two being pathogenic, N. meningitidis and N. gonorrhoeae (Seifert, 2019; Tone Tønjum, 2017). N. meningitidis asymptomatically colonizes ~10% of the world’s population, however, once pathogenic can lead to meningitidis and sepsis and lead to high fatality rates in the absence of immediate treatment (Caugant and Brynildsrud, 2020; Read, 2019; Siddiqui et al., 2021). N. gonorrhoeae causes the sexually transmitted disease gonorrhoea (Boyajian et al., 2016). The Centers for Disease Control and Prevention (CDC) have categorized N. gonorrhoeae as an urgent threat to public health, which requires immediate and aggressive actions to combat the emerging antibiotic-resistant strains recently discovered (Aitolo et al., 2021; Prevention (CDC), 2011). Although vaccines are now available against all pathogenic serogroups of N. meningitidis, there are well-documented drawbacks and limitations of these vaccines (Deasy and Read, 2011; Pizza et al., 2020). And despite decades of research, there is still no vaccine against gonococcal infections. Therefore, there is an urgent need for the development of new and improved therapeutics for protection against these human pathogens, particularly against N. gonorrhoeae.
Acquisition of nutrients is an essential step during Neisserial pathogenesis (Stork et al., 2013; Zackular et al., 2015). Iron is an essential nutrient for survival, growth, and virulence (Grifantini et al., 2003). Given that free iron is essentially non-existent in the human host, Neisseria have evolved specialized surface receptors to hijack iron from host iron-binding proteins such as transferrin (Tf) and lactoferrin (Lf) (Lee and Schryvers, 1988; Schryvers and Lee, 1989; Schryvers and Morris, 1988; Yadav et al., 2019). One of the more well-studied metal acquisition systems in Neisseria is the transferrin-binding protein (Tbp) system, which consists of an a TonB-dependent transporter called TbpA and a lipoprotein co-receptor called TbpB (Cornelissen et al., 1992; Irwin et al., 1993; Noinaj et al., 2012a; Noinaj et al., 2012b; Noinaj et al., 2010; Pintor et al., 1998). Here, TbpB serves to capture and deliver iron-loaded Tf to TbpA for iron extraction and import across the outer membrane in a process that requires energy from the Ton complex at the inner membrane (Cornelissen et al., 1992; Cornelissen and Hollander, 2011; Noinaj et al., 2010).
A second analogous system is called the lactoferrin-binding protein (Lbp) system, which is composed of LbpA and LbpB, targets Lf as an iron source (Figure 1A; Biswas and Sparling, 1995; Gray-Owen and Schryvers, 1996; Lee and Schryvers, 1988; Noinaj et al., 2013; Schryvers et al., 1998; Schryvers and Morris, 1988). However, less is known structurally and functionally about the Lbp system as no structures of Lbp proteins in complex with Lf have been reported. While all strains of N. meningitidis contain a functional Lbp system, it is only observed in roughly half of the N. gonorrhoeae strains (Anderson et al., 2003; Biswas et al., 1999). Studies hint that the Lbp system may serve as a backup option to the Tbp system to assist in mediating pathogenesis within diverse host environments, as a Neisserial strain lacking the Tbp proteins were still able to use the Lbp proteins to cause infection (Anderson et al., 2003). Further, it was also shown that a strain expressing both systems was more competitive than a strain expressing just the Tbp system alone (Anderson et al., 2003). Moreover, anti-sera raised against the Lbp proteins displays bactericidal activity, exemplifying the promise of this system as a target for therapeutic intervention (Pettersson et al., 2006).
Given its sequence homology to TbpB, LbpB is predicted to have a similar domain organization consisting of an N- and C-lobes, with each lobe composed of an eight-stranded β-barrel sitting adjacent to a handle domain (Beddek and Schryvers, 2010; Brooks et al., 2014; Ekins et al., 2004; Lee and Schryvers, 1988; Noinaj et al., 2013; Ostan et al., 2017; Schryvers and Lee, 1989). This was partially confirmed when the structure of the N-lobe of LbpB was reported, showing conservation with the N-lobe of TbpB (Brooks et al., 2014). However, reports have varied on the mode of interaction between LbpB and Lf. Docking studies predicted that N-lobe of Lf interacts with LbpB (Arutyunova et al., 2012; Brooks et al., 2014), while hydrogen-deuterium exchange studies, combined with site-directed mutagenesis, showed that the C-lobe of Lf interacts with LbpB (Ostan et al., 2017). Homology modelling studies predicted that binding would align with that observed in TbpB–Tf (Noinaj et al., 2013), however, no structure of LbpB in complex with Lf has been reported.
In addition to its role in iron acquisition, LbpB has been shown to provide protection against the host cationic antimicrobial peptide lactoferricin (Lfcn), an 11-residue metabolized fragment from the N-lobe of Lf (Figure 1A; Morgenthau et al., 2014a; Morgenthau et al., 2012; Morgenthau et al., 2014b). Studies have been conflicting on exactly where LbpB interacts with Lfcn to neutralize this threat. One model proposes that it binds along the N-lobe (Brooks et al., 2014), while another model points to several anionic regions along the C-lobe (Morgenthau et al., 2014a; Morgenthau et al., 2014b). In addition to protecting the surface of Neisseria directly, LbpB has been shown to also be proteolytically cleaved by NalP, which enables it to diffuse into the host environment to pre-emptively neutralize Lfcn (Roussel-Jazédé et al., 2010). This additional role for LbpB serving as an antimicrobial peptide sink during Neisserial pathogenesis, in conjunction with its absence in many Neisserial strains despite LbpA being present, has cast some doubt onto its importance in iron import (Morgenthau et al., 2014a).
To further characterize the role of LbpB in Neisserial pathogenesis, here we report the crystal structure of N. meningitidis LbpB (NmLbpB) in complex with Lf and the cryoEM structure of N. gonorrhoeae LbpB (NgLbpB) in complex with Lf. Our structures were further confirmed by size-exclusion chromatography small-angle X-ray scattering (SEC-SAXS) studies which also indicated that Lf and Lfcn have non-overlapping binding sites on LbpB with a 1:1 binding ratio for each. Structure-guided mutagenesis studies then probed the binding interfaces between NmLbpB and both Lf and Lfcn using biophysical and biochemical techniques. Our results support the model where LbpB serves dual functions, both in iron piracy from holo-Lf and as an antimicrobial peptide sink, during Neisserial pathogenesis.
Results
Complex formation of Neisserial LbpB with human Lf
For complex formation and structural studies, we recombinantly expressed and purified the soluble construct of LbpB from N. meningitidis (NmLbpB) (residues 20–737) and N. gonorrhoeae (NgLbpB) (residues 20–728) (Figure 1B). Solid-phase-binding assays showed that these purified LbpB constructs were sufficient for binding holo-Lf (Figure 1C and Figure 1—source data 1). For large-scale studies, Lf was purchased and further purified using SEC. Complexes of LbpB with Lf were formed upon incubation of purified LbpB with an excess of Lf and separated using SEC, with the complex eluting as a left-shifted peak (Figure 1D). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) analysis confirmed the peak contained both components and suggested a 1:1 stoichiometric complex for both the NmLbpB and NgLbpB complexes with Lf (Figure 1E and Figure 1—source data 1).
Solution studies of LbpB complexes with Lf
It has been previously reported that NmLbpB may have multiple Lf-binding sites and may form higher-order complexes (Ostan et al., 2017). However, in our studies with complex formation by SEC, we observed that NmLbpB appears to form a stable 1:1 complex with Lf. To further confirm this, we studied the stoichiometry of LbpB (Nm and Ng) in complex with Lf in solution using inline SEC-SAXS. The SAXS curve provides information about the shape, molecular weight, and maximum length of a protein or protein complex. It is a great tool for comparing the structural effects of different ligands bound LbpB and for comparing the structures of LbpB from Nm and Ng to determine how closely they match. Further, SAXS allows us to compare how closely our structures match what is observed in solution by seeing how well the simulated SAXS curve from our structure fits the experimental SAXS curve. For our experiments here, our SEC-SAXS studies showed that the individual LbpB and Lf proteins existed as monomers in solution, while the NmLbpB–Lf complex had an experimental molecular weight of 159.6 kDa corresponding to 1:1 stoichiometry complex of NmLbpB and Lf (calculated molecular weight is 156.6 kDa) (Figure 2A and B and Supplementary file 1a). The calculated radius of gyration (Rg) and maximal intramolecular dimension (Dmax) are both consistent with a 1:1 stoichiometric complex. Further, we used SEC-SAXS analysis to show that in solution, NgLbpB alone and a complex with Lf match closely to those with NmLbpB (Figure 2A and B, and SI Supplementary file 1).
The crystal structure of NmLbpB in complex with human Lf
Static small-angle X-ray scattering (SAXS) analysis showed that NmLbpB alone tends to aggregate in solution, while the NmLbpB–Lf complex forms a monodisperse stable complex (Figure 2—figure supplement 1). Therefore, crystallization screening focussed on both Nm and NgLbpB–Lf complexes using commercial broad-matrix screens. Initial lead conditions were optimized, however, diffraction quality crystals could only be grown of the NmLbpB–Lf complex. Data were collected at the GM/CA ID-D beamline at the Advanced Photon Source and the structure of the NmLbpB–Lf complex solved to 2.85 Å resolution using molecular replacement in space group P43212. Model building was performed in COOT and the structure refined using phenix.refine to final Rwork/Rfree values of 0.20/0.25 (Figure 3A and Supplementary file 1b). One complex was observed in the structure containing one molecule of Lf (consisting of ordered N- and C-lobes) and one molecule of NmLbpB (consisting of an ordered N-lobe, but partially ordered C-lobe).
The overall structure of the NmLbpB–Lf complex has an overall L-shape (Figure 3B). The N-lobe of NmLbpB is composed of residues 1–342 forming two domains, an N-terminal handle domain (residues 45–173) and an eight-stranded β-barrel domain (residues 174–342). The handle domain contains four anti-parallel β-strands and an α-helix between β-strand 1 and 2. The β-barrel domain is packed against the β-sheet of handle domain. The C-lobe of NmLbpB is composed of residues 359–718 and found largely disordered in the crystal structure as evident from higher B-factors for this region compared to the rest of the structure (Figure 3C). The C-lobe also contains a handle domain (residues 359–540) and an eight-stranded β-barrel domain (residues 541–718). While the C-lobe β-barrel domain shares homology with the N-lobe β-barrel domain, the C-lobe handle domain is different as it is composed of a six-stranded β-sheet packed against the β-barrel domain and flanked by two antiparallel β-strands. Importantly, the C-lobe of LbpB contains several long anionic loops, however, none of these were observed in the electron density likely due to their flexibility.
The Lf structure also consists of N- and C-lobes with each lobe further divided into two subdomains termed N1 and N2 (N-lobe) and C1 and C2 (C-lobe). In the presence of iron, the subdomains of each lobe are bridged together forming the closed state (Baker and Baker, 2005; Sill et al., 2016). However, in absence of iron, these lobes are known to undergo conformational changes to adopt an open conformation (Baker and Baker, 2005; Sill et al., 2016). Structural alignment of Lf alone (PDB ID 2BJJ) with Lf in our complex (RMSD 1.3 Å) shows that both lobes are in closed conformations, as expected since we observe an iron in each lobe, and that Lf undergoes minimal changes upon binding NmLbpB (Figure 3D). Further, the calculated SAXS scattering curve from our crystal structure agrees well with our experimental SAXS scattering curve in solution (Figure 2H1).
The interaction of NmLbpB with Lf is mediated exclusively through the N-lobe of NmLbpB with the C-lobe of Lf (Figure 3B). This interface consists of regions along both the C1 and C2 domains of Lf including residues D630, S637, T639, K640 (C1 domain) and E512, R525, E538, N539, D561 (C2 domain) (Figure 3B and E). Interacting simultaneously with the C1 and C2 domains of Lf, the N-lobe of NmLbpB has an extended interaction interface that includes residues N154, R193, S201, D204, N213, R223, K230, Y253, Q255, and S258 (Figure 3B and E). Given our crystal structure indicated that binding to Lf was exclusively along the N-lobe of NmLbpB, we used solid-phase-binding assays to show that indeed Lf could only interact with the full length and N-lobe only constructs, but not with the C-lobe only (Figure 3F and Figure 3—source data 1f).
The cryoEM structure of NgLbpB in complex with human Lf
Crystallization with NgLbpB was unsuccessful, therefore, we used cryoEM to determine the structure of NgLbpB in complex with Lf to 3.65 Å resolution (Supplementary file 1c). Very good density was observed for side chains of both lobes of Lf and for the N-lobe of NgLbpB allowing unambiguous model building and refinement, however, almost no density was observed for the C-lobe of NgLbpB, unless map contours were reduced drastically (Figure 4A and B and Figure 4—figure supplement 1). An alignment of the Nm and NgLbpB complexes with Lf had an overall RMSD of 3.7 Å, however, an improved RMSD of 1.4 Å when aligning just along the interacting domains (C-lobe of Lf and N-lobe of LbpB), indicating a conserved binding interface between Nm and Ng (Figure 4C and D). Additionally, the N-lobe of Lf in the NgLbpB–Lf cryoEM structure is positioned ~12° relatively to the NmLbpB–Lf crystal structure. Whether this structural difference in the N-lobe of Lf is just an artifact of crystal packing or if it is truly important for function in LbpB remains to be determined.
Mutagenesis studies targeting the binding interface between LbpB and Lf
In the Nm/NgLbpB–Lf complex structures, LbpB interacts with both the C1 and C2 subdomains within the C-lobe of Lf in an extended interface (Figures 3B, E ,, 4C and D). In the NmLbpB–Lf complex, the interaction interface is stabilized by extensive hydrogen bonding and salt bridge interactions encompassing a buried surface area of ~1760.8 Å2 (Supplementary file 1d). Based on analysis of our crystal structure, we performed mutagenesis of interface residues in NmLbpB important for mediating Lf binding (Figure 5). Those included K71, Q73, R135, K140, K143, R193, D204, T209, Y210, R223, and D227, most of which are conserved in NgLbpB (Figure 4D and Supplementary file 1e). These mutants all expressed similar to wild type and could be purified using the same method, indicating they were stably folded. The effect of these mutations on Lf binding was first tested with solid-phasing binding assays (Figure 4—figure supplement 2 and Figure 4—figure supplement 2; Source data 1) and with enzyme-linked immunosorbent assays (ELISAs) and compared to wild type (Figure 5B). The results showed that all of the mutations had a significant effect on Lf binding with most resulting in more than ~50% reduction in binding except for mutations Q73E, K143E, and R223E. Those having at least a ~75% reduction in binding included R135E, D140K, R193E, D204K, D227K, R135E/R223E, and T209N/Y210K. To further characterize Lf binding to the mutants, we performed isothermal titration calorimetry (ITC) analysis using a MicroCal iTC200 to determine thermodynamics parameters of binding, including Kd values, and compared the results of the mutants to wild type (Figure 5C and Supplementary file 1f). Representative ITC plots show a Kd value of 0.14 μM for Lf binding to wild-type NmLbpB with a ΔH of −15.8 kcal/mol and an n value of ~1. Agreeing well with the ELISA analysis, ΔH values for the all the mutants except for K143E were significantly reduced ranging from −5.4 to −11.8 kcal/mol (R135E, D140K, R193E, and D227K) and accompanied by increases in Kd values ranging from 9- to 115-fold over wild type.
Mutagenesis studies targeting the putative binding interface between LbpB and Lfcn
To gain more insight into the binding of Lfcn with LbpB, we performed SEC-SAXS experiments with and without Lfcn, finding that the presence of Lfcn produced a small, but reproducible, change in the scattering profile at lower q values in the range of 0.04–0.08 (Figure 2C and D). A similar change along lower q values in the range of 0.05–0.07 was also observed for the NmLbpB–Lf complex when Lfcn was added (Figure 2E). Together, these data support the hypothesis that Lfcn binding is distinct from Lf binding, further supporting that Lfcn binding is along the C-lobe of LbpB. While we were able to co-crystallize Lfcn with NmLbpB–Lf and solve the structure, the C-lobe of LbpB remained highly disordered and therefore, we were unable to determine the binding interactions. Therefore, to probe the putative binding site of Lfcn, we analyzed the NmLbpB–Lf structure to determine the boundaries of the C-lobe loops (Figure 6A). An electrostatic surface representation of this region shows it to be highly charged with at least one large strongly electronegative region along loops consisting of residues 372–383, 416–418, and 445–526; a more accurate analysis of the electrostatics here is not possible given the lack of order in the loops (Figure 6B).
Based on these loop boundaries, we made deletion mutants with each lacking one of the loops of the C-lobe of NmLbpB. Albeit to varying levels, these mutants all expressed and could be purified using the same method as for wild type, indicating they were stably folded. We then analyzed the effects of these deletions on Lfcn binding in comparison to wild type using ITC analysis on a Nano ITC instrument (Figure 6C and SI Table S7). Our results show that Lfcn binds to wild-type NmLbpB with a Kd of 3.7 μM, a ΔH of −11.4 kcal/mol, and an n value of ~1. The loop deletions Δ372–383, Δ416–418, Δ561–565, Δ594–599, and Δ665–698, however, showed only minimal differences in ΔH and no significant change in Kd, except for Δ445–526 (ΔH of −9.6 kcal/mol and a ~2.5-fold increase in Kd). All n values here are larger than 1 and roughly twofold higher than that observed with Lf (n value slightly below 1), an observation we attribute to inaccuracies in using absorbance measurements at λ280 for determining protein and peptide concentrations. Loops containing residues 445–526 and 665–698 contain charged patches that have been shown to affect Lfcn binding (Morgenthau et al., 2014a; Ostan et al., 2017), however, individually removing them did not appear to abrogate binding in our study. However, a double loop-deletion mutant Δ445–526_Δ665–698 showed a significantly reduced ΔH with only barely detectable binding. This confirms that these two loops together contribute to Lfcn binding in LbpB, which is likely mediated by the charged residues they contain. Exactly how Lfcn binding is mediated though by these loops will require additional structural studies.
Our SEC-SAXS experiments suggested that Lf and Lfcn had distinct, non-competing, binding sites on LbpB. Therefore, we next performed ITC experiments using a Nano ITC instrument to determine the binding properties of Lf to NmLbpB in the absence or presence of Lfcn. Our results show that Lf binds to NmLbpB alone with a Kd of 0.45 μM and a ΔH of −15.1 kcal/mol (SI Table S8). Nearly identical binding parameters are observed for Lf binding to the preformed NmLbpB–Lfcn complex, indicating that the presence of Lfcn has no effect on Lf binding (Figure 6D and SI Table S8). We next tested the effect of the presence of Lf on Lfcn binding. Our earlier results showed that Lfcn binds to NmLbpB alone with a Kd of 3.7 μM and a ΔH of −11.4 kcal/mol, while here we found that Lfcn binds to the NmLbpB–Lf complex with a Kd of 7.3 μM and a ΔH of −10.6 kcal/mol (Figure 6E and SI Table S8). Only ~ twofold difference in Kd was observed here, however, no change in ΔH indicating that binding of Lfcn to NmLbpB is not disrupted in the presence of Lf.
Discussion
The role of LbpB in Neisserial pathogenesis has been debated over the years given that it appears to share many properties with TbpB, which is involved in iron scavenging from Tf, but has also been shown to be important in protecting against the host antimicrobial peptide Lfcn (Morgenthau et al., 2014a; Morgenthau et al., 2014b; Roussel-Jazédé et al., 2010). For its iron import role, studies have indicated that LbpB may bind multiple copies of Lf (Ostan et al., 2017). However, our studies here strongly support that LbpB binds Lf in a 1:1 complex, which is demonstrated by our SEC, SAXS, X-ray crystal structure, and cryoEM structure. Further, our ITC experiments also demonstrate 1:1 binding properties with an n value (molar ratio) of ~1.
Our structures align well with the previously reported N-lobe only structure of LbpB (PDB ID 4U9C) with an RMSD of 0.34 Å (for 261 Cα atoms) compared to our NmLbpB crystal structure. Additionally, NmLbpB aligns well with NmTbpB (PDB ID 3V8U) with an overall RMSD of 1.8 Å. The interacting lobes from LbpB–Lf and TbpB–Tf (PDB ID 3VE1) have an overall RMSD of 1.4 Å, further evidence that the Lbp and Tbp systems likely share a common mechanism in iron acquisition from their respective host iron-binding proteins (Figure 4—figure supplement 3; Gray-Owen and Schryvers, 1996; Noinaj et al., 2013; Schryvers and Lee, 1989). Here, surface anchored LbpB would selectively accumulate iron-bound Lf, preserving the bound iron for eventual import by LbpA (Figures 3G and 6F; Calmettes et al., 2012). This would ensure efficiency in iron scavenging for survival during pathogenesis since TbpA (and likely LbpA) can also bind with high affinity apo-Tf/Lf which lacks iron (Krell et al., 2003; Noinaj et al., 2012b; Powell et al., 1998).
Unlike TbpB, LbpB has also been implicated in mediating evasion of the host immune responses during infection by specifically neutralizing the antimicrobial peptide Lfcn (Morgenthau et al., 2014b; Ostan et al., 2017). This may occur at the surface of the pathogen or in solution following cleavage and release by NalP (Morgenthau et al., 2014a; Roussel-Jazédé et al., 2010). Exactly how LbpB interacts with Lfcn has not been fully resolved, however, studies have pointed to several charged patches on the C-lobe as mediating this interaction (Morgenthau et al., 2014a; Ostan et al., 2017). Further, we report here that the loop containing the smaller charged region (residues 665–698) alone has little effect on Lfcn binding compared to wild type, yet does appear to synergize binding with the loop consisting of 445–526. Together, our structural studies, mutagenesis, and binding studies agree with the earlier reports and allow us to conclude that Lfcn specifically interacts with the C-lobe only of LbpB through primarily two loops consisting of residues 445–526 and 665–698. It has been shown that LbpB can protect against other peptides as well, however, whether binding of these other peptides involves the same loops or charged interfaces remains to be determined. The presence of Lf had little effect on the binding of Lfcn and vice versa, demonstrating that each have non-overlapping and non-competing binding sites, refuting the previous notion that both may share a common binding site (Brooks et al., 2014). While we were able to grow crystals in the presence of Lfcn, the loops along the putative binding interface lacked sufficient density to observe the peptide itself or its interactions with LbpB. Therefore, further studies will need to be done here in order to fully understand the atomic details of this interaction. Together, our studies support a model where LbpB serves a dual function in Neisserial pathogenesis, in both iron import under iron-limiting conditions and as an antimicrobial peptide sink for the evasion of host immune responses (Figure 6F).
Materials and methods
Cloning, expression, and purification
Request a detailed protocolThe codon optimized LbpB from N. meningitidis strain MC58 (NmLbpB) was purchased from Bio Basic. For expression, a gene fragment encoding residues 20–737 was subcloned in expression vector pHIS-Parallel2 with N-terminal 6× His-tag and Tobacco Etch Virus (TEV) protease cleavage site. For large-scale expression, the expression vector was transformed into BL21(DE3) cells and protein expression performed at 24°C upon induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 9–12 hr. Cells were harvested by centrifugation at 6000 rpm and stored at −80°C until further use.
For purification, frozen pellets were thawed and resuspended in lysis buffer (20 mM Tris–HCl, pH 7.5, 150 mM NaCl, 5 mM β-mercaptoethanol [BME], 25 mM imidazole) supplemented with 10 µg/ml DNaseI and 0.5 mM phenylmethylsulfonyl fluoride. Cells were lysed by three passages through Emulsiflex C3 high pressure homogenizer (Avestin). The cellular debris were removed by centrifugation at 18,000 rpm for 30 min at 4°C. The supernatant was collected and subjected to affinity purification using 5 mL Hi-Trap column containing Ni-NTA resin (Qiagen) on an AKTA Pure 25 L protein purification system (GE Healthcare). The column was washed with 10 column volume (CV) lysis buffer and protein was eluted using elution buffer (20 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM BME, 250 mM imidazole). The eluted protein was subjected to TEV protease digestion to remove the 6× His-tag. The digested protein was cleaned up using a Ni-NTA affinity column and the flowthrough collected. The concentrated flowthrough was further purified using SEC on a Superdex 200 10/300 GL Increase (GE Healthcare) column using 20 mM Tris–HCl pH 7.5, 150 mM NaCl. The elution fractions were run on an SDS–PAGE gel to examine purity. The fractions containing NmLbpB were pooled and concentrated for further experiments.
The DNA sequences for the individual lobes (N- and C-lobes) encoding residues 20–362 and 381–737, respectively, were amplified using full-length NmLbpB as template. These fragments were ligated into pHIS-Parallel2 with N-terminal 6× His-tag and TEV protease cleavage site. Also, point mutants were created using wild-type NmLbpB plasmid using standard site-directed mutagenesis methods. These constructs were expressed and purified using the similar approach as full-length NmLbpB as described above.
The NgLbpB gene fragment encoding residues 20–728 was amplified from the genomic DNA of N. gonorrhoeae strain FA19 with primers containing restriction sites for NcoI and XhoI. The gene fragment was cloned into pHIS-Parallel2 plasmid for expression. The construct was confirmed by DNA sequencing. The expression and purification of NgLbpB were performed using the same approach as for full-length NmLbpB as described above.
For complex formation, purified Nm/NgLbpB was incubated with twofold molar excess of human holo-Lf (RayBiotech, Inc) at 4°C for 1 hr. The complex was separated from excess Lf using SEC on a Superdex 200 10/300 GL Increase column (GE Healthcare). Complex formation was confirmed using SDS–PAGE gel. The fractions containing Nm/NgLbpB–Lf were pooled and concentrated for further experiments.
Dot blots
Request a detailed protocolFor dot blot assays with NmLbpB wild type and mutants, 10 µl of each sample was spotted on polyvinylidene difluoride (PVDF) membrane and air dried. The membrane was then blocked by incubation of the membrane in 5% bovine serum albumin (BSA) for 1 hr followed by three washes with 1× PBST (1× phosphate buffered saline [PBS] + 0.005% Tween-20) 5 min each. Potential ligand binding was performed by incubating the blots in 20 µg/ml holo-Lf in PBST +0.5% BSA for 1 hr. Unbound ligand was removed by three washes with PBST. The blots were probed using horse radish peroxidase (HRP) enzyme-conjugated rabbit anti-human Lf antibody (RayBiotech, Inc) for 1 hr. Excess antibody was removed by washing using three washes of 1× PBST and 1× PBS each. Finally, enhanced chemiluminescence (ECL) substrate was used for the detection of HRP’s enzymatic activity.
For dot blot assays with NgLbpB wild type and mutants, 10 µl of each sample was spotted on activated PVDF membrane and air dried. 1× PBS with BSA (5%) was used for blocking unoccupied sites followed by three washes with 1× PBST. The HRP-conjugated holo-Lf (Lf-HRP) probe was used to monitor Lf binding. Excess probe was removed by washes with 1× PBST and 1× PBS. Finally, ECL substrate was used for the detection of HRP activity.
All dot blot assays were performed at least in triplicate with representative data shown.
Size-exclusion chromatography small-angle X-ray scattering
Request a detailed protocolPurified samples were subjected to inline SEC-SAXS. The scattering data were collected at beamline 18-ID of Biophysics Collaborative Access Team (BioCAT) of the Advanced Photon Source, Argonne National Laboratory. The data were analyzed and final plots made using BioXTAS RAW (Hopkins et al., 2017) and ATSAS (Manalastas-Cantos et al., 2021). First, the data were reduced and the data range for scattering curves were selected. Upon averaging of the data, the q-range and molecular weight information were obtained by analyzing Guinier plot. The pair-distance distribution curves were calculated using GNOM. Theoretical scattering for the X-ray and cryoEM structures was calculated and compared with experimental scattering curves using Crysol.
Crystallization, data collection, and structure determination using X-ray crystallography
Request a detailed protocolFor crystallization, NmLbpB–Lf was concentrated to 7.4 mg/ml. Initial crystallization trials were performed using commercial screens using hanging-drop vapour-diffusion method at 20°C. Crystal hits identified from these screens were optimized. Diffraction quality crystals of NmLbpB–Lf were obtained in 2.0 M ammonium sulphate, 20 mM Tris, pH 8.5 at 20°C. Crystals were harvested and diffraction data were collected at the GM/CA 23ID-D beamline at the Advanced Photon Source, Argonne National Laboratory using Pilatus 6 M detector at 100 K. The data were processed using HKL2000 (Otwinowski and Minor, 1997) in space group P43212 with unit cell parameters a = b = 120.39, c = 207.38, α = β = γ = 90.0.
Initial phases were calculated by molecular replacement within PHASER (Phenix) (Liebschner et al., 2019; McCoy et al., 2007) using the structure of diferric human Lf (PDB ID: 2BJJ) and structure of the N-lobe of NmLbpB (PDB ID: 4U9C). Furthermore, the model was build using Coot (Emsley et al., 2010) and refined using phenix.refine (Phenix) (Liebschner et al., 2019). The final model was refined to Rwork and Rfree values 0.20 and 0.25, respectively, at 2.85 Å resolution. The geometric analysis was performed using Molprobity (Phenix) (Chen et al., 2010; Liebschner et al., 2019). Structural analysis and figure preparation were performed using PyMol (Schrödinger). Final figures were assembled using Adobe Photoshop and Illustrator.
Grid preparation, data collection, and structure determination using cryoEM
Request a detailed protocolPurified NgLbpB–Lf complex was applied to Quantifoil R 3.5/1 Cu 200 grids, that were first glow discharged using a Pelco EasiGlow instrument, and plunge-frozen using Vitrobot Mark IV (Thermo Fisher Scientific). Grids were screened and the grid with optimal particle distribution and ice-thickness was used for data collection using a Titan Krios G1 microscope equipped with a Gatan K3 direct electron detector and a Gatan Quantum energy filter. Movies were collected in super-resolution counting mode (pixel size 0.54 Å) with a total dose of 53.68 e−/Å2. A total 4966 movies were collected and subjected to motion correction using MotionCor2 (Zheng et al., 2017). The subsequent data processing was performed using cryoSPARC (Punjani et al., 2017). CTF parameters were obtained using ‘Patch CTF estimation (multi)’. An initial set of templates were made by first using ‘Blob picker’ followed by ‘2D Classification’. The best 2D classes were then fed into ‘Template picker’ and subjected to multiple rounds of 2D classification to remove junk particles. The best classes representing different particle orientation were then used for ‘Ab-initio Reconstruction’. After two rounds of ‘Ab-initio Reconstruction’ followed by ‘Heterogeneous refinement’, the best-looking class was subjected to ‘Non-uniform Refinement’, which yielded a final reconstruction to 3.65 Å resolution. A model of the NgLbpB–Lf structure was prepared based on the NmLbpB–Lf crystal structure and placed and fit within the map using ChimeraX (Pettersen et al., 2021). The cryoEM map contained well-resolved density at the protein–protein interface, however, the C-lobe of NgLbpB could only be placed as a rigid body given only minimal density even when map contours were reduced. The structure was refined using ‘Real-space refinement’ within Phenix (Liebschner et al., 2019) and figures and analysis performed using ChimeraX (Pettersen et al., 2021) and PyMOL (Schrödinger). Final figures were assembled using Adobe Photoshop and Illustrator.
Enzyme-linked immunosorbent assays
Request a detailed protocolFor binding analysis with Lf, we performed sandwich ELISAs. The NmLbpB variants were coated on hydrophobic plates by first incubating the plates with 0.1 mg/ml of each protein at 4°C overnight. Acinetobacter baumannii BamB (a kind gift from Robert Stephenson) was included as a negative control. After incubation, wells were washed three times with 1× PBST and blocked with 5% BSA for 30 min. Subsequently, BSA was removed and the wells were washed three times with 1× PBST. For ligand binding, the wells were incubated with the Lf for 30 min. Following that, plates were washed three times with 1× PBST and probed with HRP-conjugated rabbit anti-human Lf antibody (RayBiotech, Inc) for 30 min. The antibody was washed away with three washes of 1× PBST and 1× PBS. The assay was then developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Scientific). Finally, the assay was stopped upon addition of 0.18 M sulfuric acid. For quantitative data, the absorbance at 450 nm was measured using a Spectramax M2e microplate reader (Molecular Devices). All the experiments were performed in triplicate and repeated at least twice. Similarly, to study Lf binding of the NgLbpB wild type and mutants, ELISAs were performed using HRP-conjugated holo-Lf (Lf-HRP) as a probe for Lf binding. Pisum sativum Toc75 POTRA (a kind gift from Karthik Srinivasan) was included as a negative control.
All ELISA experiments were performed in triplicate with standard errors shown.
Isothermal titration calorimetry
Request a detailed protocolTo monitor the properties of Lf binding to the NmLbpB variants, ITC experiments were performed using a MicroCal iTC200 ITC calorimeter (Malvern Panalytical). The NmLbpB variants and Lf proteins were buffer exchanged into 1× PBS (PBS: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.5) by SEC. The NmLbpB variants and Lf were concentrated to 30 and 300 µM concentration, respectively. Lf was titrated into ITC sample cell containing the NmLbpB variants at 25°C. Data analysis was performed using Origin 7.0 (OriginLab).
Lfcn binding to wild type and loop-deletion mutants of NmLbpB was measured using a Nano ITC instrument (TA instruments). The sample cell contained 30 µM of protein with 600 µM of Lfcn injected over 20 injections with stirring at 300 RPM at a temperature of 10°C. The ITC data were analyzed using minimized independent binding model with the instrument NanoAnalyze software.
ITC experiments to study the effect of presence of Lfcn on Lf binding to NmLbpB and vice versa, were performed using a Nano ITC instrument (TA instruments). The protein in the sample cell was loaded at 30 µM concentration with stirring at 300 rpm at 25°C for Lf and at 10°C for Lfcn titrations at concentrations of 300 and 600 µM, respectively. The ITC data were analyzed using a minimized independent binding model using the instrument NanoAnalyze software.
All ITC experiments were performed with at least two replicates with representative data shown.
Data availability
All coordinates, structure factors, and cryo-EM maps have been uploaded to the PDB and/or the EMDB as follows: X-ray structure of NmLbpB in complex with human lactoferrin, PDB 7JRD; Cryo-EM structure of NgLbpB in complex with human lactoferrin, PDB 7N88 (EMD-24233).
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RCSB Protein Data BankID 7JRD. The crystal structure of lactoferrin binding protein B (LbpB) from Neisseria meningitidis in complex with human lactoferrin.
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RCSB Protein Data BankID 7N88. The cryoEM structure of LbpB from N. gonorrhoeae in complex with lactoferrin.
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Electron Microscopy Data BankID EMD-24233. The cryoEM structure of LbpB from N. gonorrhoeae in complex with lactoferrin.
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Article and author information
Author details
Funding
Purdue Research Foundation
- Nicholas Noinaj
- Ravi Yadav
Department of Biological Sciences, Purdue University
- Nicholas Noinaj
- Ravi Yadav
Indiana Clinical and Translational Sciences Institute
- Nicholas Noinaj
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank the Noinaj Lab for advice and helpful discussions on these studies. We thank Dr. Thomas Klose (Purdue CryoEM Facility) and Runrun Wu (Noinaj Lab) for their assistance with collection of the cryoEM data. This work was supported by the Purdue Research Foundation (RY) and by the Department of Biological Sciences (NN). The cryoEM studies were funded, in part, with support from the Indiana Clinical and Translational Sciences Institute funded, in part by Grant Number UL1TR002529 from the NIH, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award. We also thank the beamline staffs of the BioCAT and GM/CA beamlines at the Advanced Photon Source (APS), Argonne National Laboratory for their help with data collection. GM/CA@APS has been funded by the National Cancer Institute (ACB-12002) and the National Institute of General Medical Sciences (NIGMS) (AGM-12006, P30GM138396). The BioCAT resources are supported by grant P30 GM138395 from the NIGMS of the NIH; use of the Pilatus 3 1 M detector was provided by grant 1S10OD018090 from NIGMS. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.
Copyright
© 2021, Yadav et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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Further reading
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Background:
Under which conditions antibiotic combination therapy decelerates rather than accelerates resistance evolution is not well understood. We examined the effect of combining antibiotics on within-patient resistance development across various bacterial pathogens and antibiotics.
Methods:
We searched CENTRAL, EMBASE, and PubMed for (quasi)-randomised controlled trials (RCTs) published from database inception to 24 November 2022. Trials comparing antibiotic treatments with different numbers of antibiotics were included. Patients were considered to have acquired resistance if, at the follow-up culture, a resistant bacterium (as defined by the study authors) was detected that had not been present in the baseline culture. We combined results using a random effects model and performed meta-regression and stratified analyses. The trials’ risk of bias was assessed with the Cochrane tool.
Results:
42 trials were eligible and 29, including 5054 patients, qualified for statistical analysis. In most trials, resistance development was not the primary outcome and studies lacked power. The combined odds ratio for the acquisition of resistance comparing the group with the higher number of antibiotics with the comparison group was 1.23 (95% CI 0.68–2.25), with substantial between-study heterogeneity (I2=77%). We identified tentative evidence for potential beneficial or detrimental effects of antibiotic combination therapy for specific pathogens or medical conditions.
Conclusions:
The evidence for combining a higher number of antibiotics compared to fewer from RCTs is scarce and overall compatible with both benefit or harm. Trials powered to detect differences in resistance development or well-designed observational studies are required to clarify the impact of combination therapy on resistance.
Funding:
Support from the Swiss National Science Foundation (grant 310030B_176401 (SB, BS, CW), grant 32FP30-174281 (ME), grant 324730_207957 (RDK)) and from the National Institute of Allergy and Infectious Diseases (NIAID, cooperative agreement AI069924 (ME)) is gratefully acknowledged.
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The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search for natural compounds deployed by microbes to inhibit competitors. Here, we show that the iron-chelating pyoverdines, siderophores produced by environmental Pseudomonas spp., have strong antibacterial properties by inducing iron starvation and growth arrest in pathogens. A screen of 320 natural Pseudomonas isolates used against 12 human pathogens uncovered several pyoverdines with particularly high antibacterial properties and distinct chemical characteristics. The most potent pyoverdine effectively reduced growth of the pathogens Acinetobacter baumannii, Klebsiella pneumoniae, and Staphylococcus aureus in a concentration- and iron-dependent manner. Pyoverdine increased survival of infected Galleria mellonella host larvae and showed low toxicity for the host, mammalian cell lines, and erythrocytes. Furthermore, experimental evolution of pathogens combined with whole-genome sequencing revealed limited resistance evolution compared to an antibiotic. Thus, pyoverdines from environmental strains have the potential to become a new class of sustainable antibacterials against specific human pathogens.