1. Structural Biology and Molecular Biophysics

Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)

  1. David J Yanofsky
  2. Justin M Di Trani
  3. Sylwia Król
  4. Rana Abdelaziz
  5. Stephanie A Bueler
  6. Peter Imming  Is a corresponding author
  7. Peter Brzezinski  Is a corresponding author
  8. John L Rubinstein  Is a corresponding author
  1. Molecular Medicine Program, The Hospital for Sick Children, Canada
  2. Department of Medical Biophysics, The University of Toronto, Canada
  3. Department of Biochemistry and Biophysics, Stockholm University, Sweden
  4. Department of Pharmaceutical/Medicinal Chemistry and Clinical Pharmacy, Martin-Luther-Universitaet Halle-Wittenberg, Germany
  5. Department of Biochemistry, The University of Toronto, Canada
https://doi.org/10.7554/eLife.71959
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Cite this article
  1. David J Yanofsky
  2. Justin M Di Trani
  3. Sylwia Król
  4. Rana Abdelaziz
  5. Stephanie A Bueler
  6. Peter Imming
  7. Peter Brzezinski
  8. John L Rubinstein
(2021)
Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)
eLife 10:e71959.
https://doi.org/10.7554/eLife.71959
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Abstract

The imidazopyridine telacebec, also known as Q203, is one of only a few new classes of compounds in more than 50 years with demonstrated antituberculosis activity in humans. Telacebec inhibits the mycobacterial respiratory supercomplex composed of complexes III and IV (CIII2CIV2). In mycobacterial electron transport chains, CIII2CIV2 replaces canonical CIII and CIV, transferring electrons from the intermediate carrier menaquinol to the final acceptor, molecular oxygen, while simultaneously transferring protons across the inner membrane to power ATP synthesis. We show that telacebec inhibits the menaquinol:oxygen oxidoreductase activity of purified Mycobacterium smegmatis CIII2CIV2 at concentrations similar to those needed to inhibit electron transfer in mycobacterial membranes and Mycobacterium tuberculosis growth in culture. We then used electron cryomicroscopy (cryoEM) to determine structures of CIII2CIV2 both in the presence and absence of telacebec. The structures suggest that telacebec prevents menaquinol oxidation by blocking two different menaquinol binding modes to prevent CIII2CIV2 activity.

Introduction

Numerous bacteria from the strictly aerobic genus Mycobacterium are human pathogens. In particular, infection by Mycobacterium tuberculosis and closely related species results in the disease tuberculosis (TB). In most years, TB is the leading cause of death by infectious disease internationally, with an increasing incidence of drug-resistant infections (Global Tuberculosis Report, 2020). Nontuberculosis mycobacterial pathogens include Mycobacterium leprae, which causes leprosy, Mycobacterium ulcerans, which causes Buruli ulcer, and Mycobacterium avium and Mycobacterium abscessus, which infect immunocompromised and cystic fibrosis patients, respectively. The discovery of bedaquiline from a phenotypic screen with non-pathogenic Mycobacterium smegmatis, and its subsequent development into an effective therapeutic, has revolutionized the treatment of multidrug-resistant and extensively drug-resistant TB (Global Tuberculosis Report, 2020; World Health Organization, 2019). Bedaquiline binds the membrane region of mycobacterial ATP synthase (Andries et al., 2005; Guo et al., 2021; Preiss et al., 2015), blocking proton translocation and ATP synthesis. Thus, in addition to providing an invaluable therapeutic tool, bedaquiline established oxidative phosphorylation as a target space for antibiotics against mycobacteria. Subsequent to the discovery of bedaquiline, numerous compounds have been identified that target either ATP synthase or the electron transport chain complexes that establish the transmembrane proton motive force (PMF) that drives ATP synthesis (Cook et al., 2017).

In mammalian mitochondrial electron transport chains, complexes I and II oxidize NADH (the reduced form of nicotinamide adenine dinucleotide) and succinate, respectively. The electrons from these substrates are used to reduce a membrane-bound pool of ubiquinone (UQ) to ubiquinol (UQH2). Electrons from UQH2 are then passed successively to CIII (also known as cytochrome [cyt.] bc1), soluble cyt. c, and CIV (also known as cyt. c oxidase or cyt. aa3) before ultimately reducing molecular oxygen to water. Complexes I, III, and IV couple electron transfer to proton transfer across the membrane, thereby generating the PMF that drives ATP synthesis. In contrast to mitochondria and many bacteria, mycobacteria possess a branched electron transport chain (reviewed in Cook et al., 2017). Rather than UQ, mycobacterial respiration relies on menaquinone (MQ) as an intermediate electron carrier. MQH2 can reduce molecular oxygen via two MQH2:O2 oxidoreductases: cyt. bd and cyt. bcc-aa3, the latter being equivalent to a combination of canonical CIII and CIV with the stoichiometry CIII2CIV2. The CIII2CIV2 supercomplex and cyt. bd branches of the mycobacterial electron transport chain are bioenergetically not equivalent, while CIII2CIV2 transfers three protons across the membrane for each electron used to reduce O2, cyt. bd transfers the equivalent of only one proton across the membrane for each electron. Enzyme utilization in mycobacterial electron transport chains can adapt to changes in environmental conditions and treatment with respiratory complex inhibitors (Arora et al., 2014; Berney and Cook, 2010), which complicates targeting of respiration by antimycobacterial drugs (Beites et al., 2019).

Structural analysis of the CIII2CIV2 supercomplex from M. smegmatis led to the discovery that a dimeric type C superoxide dismutase (SOD) is an integral component of the assembly (Gong et al., 2018; Wiseman et al., 2018). The SOD dimer is found on the periplasmic side of CIII2CIV2 and is held in place by its two N-terminal tails, which bind to the complex’s QcrA subunits. Both QcrA and the SOD subunits are highly conserved between M. smegmatis and M. tuberculosis, with 81.4% and 71.0% sequence similarity and 73.2% and 64.8% sequence identity, respectively, suggesting that a similar association occurs in the M. tuberculosis enzyme. M. tuberculosis mutants that lack SOD are susceptible to killing within macrophages (Piddington et al., 2001), emphasizing the importance of the subunit. Given its position within CIII2CIV2, it is possible that the SOD subunit abstracts electrons from reactive oxygen species formed during respiration or generated by host-defense mechanisms in the phagolysosome upon phagocytosis of M. tuberculosis and directs them through the respiratory chain to contribute to the PMF and ATP synthesis (Gong et al., 2018; Wiseman et al., 2018).

Although killing of M. tuberculosis with electron transport chain inhibitors may require simultaneously blocking both the CIII2CIV2 and cyt. bd branches for oxygen reduction (Arora et al., 2014; Beites et al., 2019; Matsoso et al., 2005), high-profile candidate TB therapeutics have been identified that bind to CIII within CIII2CIV2 (Pethe et al., 2013; Rybniker et al., 2015). Similarly, while CIII2CIV2 is not essential in M. smegmatis, its disruption causes severe growth defects (Matsoso et al., 2005). In contrast, CIII2CIV2 is essential in M. leprae and M. ulcerans, which lack the cyt. bd branch of the electron transport chain entirely (Cole et al., 2001; Demangel et al., 2009).

Rather than pumping protons, CIII contributes to the PMF by separating positive and negative charges across the membrane through a mechanism known as the Q cycle (reviewed in Sarewicz and Osyczka, 2015; Xia et al., 2013). Each CIII contains two sites where redox reactions with MQ occur: a QP site near the positive (periplasmic) side of the membrane where MQH2 is oxidized and a QN site near the negative (cytoplasmic) side of the membrane where MQ is reduced. In the current understanding of the Q cycle in mycobacteria, oxidation of MQH2 in the QP site leads to release of two protons to the positive side of the membrane. The first electron from this oxidation reaction is passed to a [2Fe–2S] Rieske center (FeS) in subunit QcrA where it consecutively reduces the cyt. cc domain of the QcrC subunit and the CuA di-nuclear center of CIV. The second electron from the MQH2 in the QP site is transferred first to heme bL and then heme bH, both in subunit QcrB of CIII, before reducing a MQ molecule in the QN site to MQ•-. Oxidation of a second MQH2 in the QP site and repetition of this series of events lead to reduction of MQ•- to MQH2 in the QN site, upon abstraction of two protons from the negative side of the membrane, thereby contributing to the PMF and the pool of reduced MQH2 in the membrane. Within CIV, electrons are transferred from the CuA di-nuclear center to O2 bound at the heme a3-CuB binuclear catalytic site via heme a, driving proton pumping across the membrane.

Telacebec (also known as Q203) was identified in a screen of macrophages infected with M. tuberculosis (Pethe et al., 2013). Generation of resistance mutants bearing T313I and T313A mutations in the qcrB gene indicated that telacebec targets CIII of the CIII2CIV2 supercomplex. A recent Phase 2 clinical trial demonstrated a decrease in viable mycobacterial sputum load with increasing dose of telacebec, supporting further development and making telacebec one of only a few new drug classes in more than 50 years with demonstrated antituberculosis activity in humans (de Jager et al., 2020). Telacebec may also have clinical utility in treating nontuberculosis mycobacterial infections, such as Buruli ulcer (Almeida et al., 2020; Van Der Werf et al., 2020).

Here, we use electron cryomicroscopy (cryoEM) to investigate how telacebec inhibits purified CIII2CIV2 from M. smegmatis. We develop conditions for CIII2CIV2 activity assays that limit the spontaneous autoxidation of MQH2 analogues, which has hampered previous analysis of MQH2:O2 oxidoreductase activity with purified CIII2CIV2. The assays show that telacebec inhibits CIII2CIV2 activity at concentrations comparable to those that inhibit electron transfer in mycobacterial membranes. CryoEM of CIII2CIV2 demonstrates both the presence of the LpqE subunit (Gong et al., 2018) and different conformations of the cyt. cc domain (Wiseman et al., 2018), which have previously been observed separately but not together. Three-dimensional variability analysis (3DVA) of the structure shows that the SOD subunit can move toward cyt. cc, supporting the possibility of direct electron transfer from superoxide to CIV. CryoEM of the CIII2CIV2:telacebec complex allows localization of the telacebec binding site with the imidazopyridine moiety and A-benzene ring of telacebec forming most protein-inhibitor contacts.

Results

Structure of CIII2CIV2 reveals movement of SOD subunit and cyt. cc domain

In order to facilitate isolation of CIII2CIV2, we used the ORBIT (oligonucleotide-mediated recombineering followed by Bxb1 integrase targeting) strategy (Murphy et al., 2018) to introduce sequence for a 3×FLAG affinity tag into the chromosomal DNA of M. smegmatis immediately 3′ to the qcrB gene. While M. smegmatis is typically grown in 7H9 medium supplemented with albumin, dextrose, and sodium chloride (ADS), we found that supplementing instead with tryptone, dextrose, and sodium chloride (TDS), which is more economical for large-scale culture, gave equivalent or superior growth. Purification of CIII2CIV2 from M. smegmatis grown in these conditions gave a high yield of enzyme with clear bands on an SDS-PAGE gelfor most of the known subunits of the complex (Figure 1A). We observed that following affinity purification, gel filtration chromatography of the enzyme led to depletion of the LpqE and SOD subunits (Figure 1A, right) compared to affinity purification alone (Figure 1A, left), and consequently this purification step was avoided.

Figure 1 with 3 supplements see all
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Structure of the Mycobacterium smegmatis CIII2CIV2 respiratory complex.

(A) SDS-PAGE shows most of the known subunits of the complex and indicates that the superoxide dismutase (SOD) and LpqE subunits are depleted by gel filtration chromatography. (B) Electron cryomicroscopy (CryoEM) map of the CIII2CIV2. The different density thresholds for the SOD subunit and the rest of the complex are indicated. Scale bar, 50 Å. (C) Three-dimensional variability analysis indicates two different clusters of particle images (‘cluster 1’ and ‘cluster 2’) that show the SOD subunit in different positions over the twofold symmetry axis of the complex. Scale bar, 50 Å. (D) An atomic model for the CIII2CIV2 complex with SOD fitted into the map and showing one half of the complex missing the LpqE subunit and with the cyt. cc domain in the ‘open’ conformation and the other half of the complex possessing the LpqE subunit and with the cyt. cc domain in the ‘closed’ conformation.

CryoEM of the CIII2CIV2 preparation allowed for calculation of a 3D map of the enzyme at a nominal resolution of 3.0 Å (Figure 1B, Figure 1—figure supplement 1, and Table 1). The map shows strong density for the LpqE subunit (Figure 1B, orange). LpqE was observed in one previous structural study of CIII2CIV2 from M. smegmatis (Gong et al., 2018) but was absent in another (Wiseman et al., 2018), presumably due to depletion of the subunit during purification of the supercomplex. In the structure missing LpqE, the cyt. cc domain of subunit QcrC adopts both an ‘open’ and a ‘closed’ conformation, while the structure with LpqE was found only in the closed conformation. The closed conformation creates a direct electronic connection between heme cII of CIII and CuA of CIV (Gong et al., 2018; Wiseman et al., 2018). In the open conformation, heme cII from the cyt. cc domain is too far from CuA to allow electron transfer, leading to the hypothesis that switching between the closed and open conformations plays a role in controlling the flow of electrons through the supercomplex (Wiseman et al., 2018). In contrast, LpqE was hypothesized to strengthen the physical attachment between CIII and CIV (Gong et al., 2018). 3DVA with the current dataset (Punjani and Fleet, 2021), focused on one half of the supercomplex, revealed complexes with and without LpqE. Where LpqE was missing, the cyt. cc domain exhibits the open conformation, while complexes with LpqE show only the closed conformation of cyt. cc (Figure 1—figure supplement 2, Video 1). Clashes between LpqE and the open conformation of cyt. cc suggest that LpqE prevents the open conformation.

Table 1
Electron cryomicroscopy (CryoEM) structure determination.
A. CryoEM data acquisition and image processing
Data collection
Electron microscopeTitan Krios G3
CameraFalcon 4
Voltage (kV)300
Nominal magnification75,000
Calibrated pixel size (Å)1.03
Total exposure (e/Å2)43.5
Exposure rate (e/pixel/s)5.99
Number of exposure fractions29
Defocus range (μm)0.7–2
Image processing
Motion correction softwarecryoSPARC v3
CTF estimation softwarecryoSPARC v3
Particle selection softwarecryoSPARC v3
Micrographs used in inhibitor-free dataset4308
Micrographs used in telacebec-bound dataset2793
Particle images selected in inhibitor-free dataset1,037,709
Particle images selected in telacebec-bound dataset387,777
3D map classification and refinement softwarecryoSPARC v3
B. Model statistics
DatasetInhibitor-freeTelacebec-bound
Associated PDB ID
Modeling and refinement softwareCoot, phenix, ISOLDECoot, phenix, ISOLDE
Protein residues60586075
Ligand9 XX: 4, 9Y0: 6, 9YF: 8, FES: 2, HEC: 4, HEA: 4, MQ9: 10, HEM: 4, PLM: 4, CU: 89 XX: 4, 9Y0: 6, 9YF: 8, FES: 2, HEC: 4, HEA: 4, MQ9: 10, HEM: 4, PLM: 4, CU: 8, QTE: 1
RMSD bond length (Å)0.0050.004
RMSD bond angle (°)0.7120.818
Ramachandran outliers (%)0.220.2
Ramachandran favored (%)92.5891.52
Rotamer outliers (%)00
Clash score19.2915.47
MolProbabity score2.252.20
EMRinger score3.122.61
Video 1
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Three-dimensional variability analysis of CIII2CIV2 showing the presence of LpqE with the cyt.

cc subunit in the closed position or the absence of LpqE with the cyt. cc subunit in the open position. Subunits are colored as in Figure 1. Please view as a loop.

In previous studies, the SOD subunit of CIII2CIV2 was poorly resolved in cryoEM maps and appeared as a diffuse density (Gong et al., 2018; Wiseman et al., 2018). In the present map, the overall shape of SOD, although still at lower density than the rest of the complex, was more readily apparent (Figure 1B, pink). The N-terminal anchors from the SOD dimer that bind to subunit QcrB are well resolved and terminate at the middle of the complex with a lipid anchor (Figure 1B, beige). The improved density for the SOD subunit allowed fitting of a homology model of the protein into the map with a slight rotation relative to how it was fit previously (Figure 1C, right). 3DVA (Figure 1C, Video 2) shows that SOD moves between the center of the complex, where it was observed previously (Gong et al., 2018; Wiseman et al., 2018) to immediately above heme cI. This proximity suggests that SOD may indeed transfer electrons abstracted from superoxide in the periplasm of M. smegmatis to CIV to contribute to the PMF (Gong et al., 2018; Wiseman et al., 2018), although this hypothesis requires further testing. The overall resolution of the map, which is somewhat better than in previous studies, allowed refinement of an atomic model for CIII2CIV2 including residues T82, E83, A123 to D131, and S183 from LpqE, and residues H57 to G78 from MSMEG_4693 (also known as CtaJ), which could not be modeled previously (Figure 1D, Figure 1—figure supplement 3, and Table 1). The model shown in Figure 1D illustrates one cyt. cc domain in the closed position with LpqE present (Figure 1D, right side: cyan and orange) and the other cyt. cc domain in the open position without LpqE (Figure 1D, left side: cyan), although other combinations could also be modeled.

Video 2
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Three-dimensional variability analysis showing movement of superoxide dismutase (SOD) subunit of CIII2CIV2.

Subunits are colored as in Figure 1. Please view as a loop.

Nanomolar telacebec inhibits oxidoreductase activity with purified CIII2CIV2

To investigate inhibition of CIII2CIV2 by telacebec, we established a supercomplex activity assay, based on measurement of oxygen consumption with a Clark-type electrode. The mycobacterial electron transport chain uses MQH2 as the electron donor for CIII2CIV2 while in canonical mitochondrial electron transport chains UQH2 donates electrons to CIII2 (Cook et al., 2017). Both UQH2 and MQH2 are insoluble in aqueous solution and consequently soluble analogues must be employed as substrates in assays with detergent-solubilized enzymes.

The midpoint potentials of the redox centers in mycobacterial CIII2 are lower than those of canonical mitochondrial CIII2 (Kao et al., 2016), and as a result UQH2 analogues typically used in CIII2 assays are not able to reduce CIII2 of the M. smegmatis supercomplex. MQH2 analogues (Figure 2—figure supplement 1A-C) capable of reducing CIII2CIV2 suffer from autoxidation at neutral or basic pH, which leads to oxygen reduction even in the absence of enzyme (Munday, 2000). This background oxygen-reduction rate is typically subtracted from oxygen reduction observed in the presence of enzyme to calculate the enzyme-catalyzed oxidoreductase activity. Previous measurement of CIII2CIV2 activity employed 2-methyl-[1,4]naphthohydroquinone (menadiol) (Gong et al., 2018) or 2,3-dimethyl-[1,4]naphthohydroquinone (DMWH2) (Graf et al., 2016; Wiseman et al., 2018) as the electron donor. Although both substrates are susceptible to autoxidation, the rate of autoxidation was proposed to be ~30% slower for DMWH2 compared to menadiol at the pH 7.5 of our oxygen consumption assays (Munday, 2000). In contrast to these earlier studies, we found that menaquinol autoxidized more slowly than DMWH2 (Figure 2—figure supplement 1D). However, we also found that mendiol was substantially less efficient than DMWH2 as an electron donor for CIII2CIV2, likely due to its less favorable redox potential (Fieser and Fieser, 1934), supporting the choice of DMWH2 as the substrate in assays (Figure 2—figure supplement 1D).

Initial activity assays led to anomalous results where addition of low concentrations of CIII2CIV2 to the assay mixture appeared to decrease the rate of oxygen reduction below the background autoxidation rate. On subsequent investigation, we realized that at low concentrations of CIII2CIV2 the SOD subunit suppresses autoxidation of DMWH2 more than CIII2CIV2 catalyzes oxidation of DMWH2. This suppression of quinol autoxidation by SOD, which has been described previously (Cadenas et al., 1988), can lead to apparent negative activities for CIII2CIV2 when the background autoxidation is subtracted. Autoxidation of quinols is believed to involve a superoxide anion intermediate, with the SOD-catalyzed dismutation of the intermediate to hydrogen peroxide removing this reactant to slow the process (Munday, 2000). To remove error introduced by the effect of the SOD subunit on the observed oxygen reduction signal, we established that bovine C-type SOD can similarly limit the autoxidation of DMWH2 (Figure 2A, blue and orange curves), as well as menadiol (Figure 2—figure supplement 1D). Thus, by adding an excess of exogenous bovine SOD to assays, the CIII2CIV2’s DMWH2:O2 oxidoreductase activity can be measured with suppression of DMWH2 autoxidation (e.g. Figure 2A, green). With 500 nM SOD added, the CIII2CIV2’s DMWH2:O2 oxidoreductase activity was measured at 91 ± 4 e-/s (± s.d., n = 6 independent assays with three each from two separate batches of protein), which is nearly an order of magnitude greater than the apparent activity found previously (Wiseman et al., 2018). We subsequently added 500 nM bovine SOD to all assays to limit autoxidation of DMWH2.

Figure 2 with 1 supplement see all
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Assay of 2,3-dimethyl-[1,4]naphthohydroquinone (DMWH2:O2) oxidoreductase activity of CIII2CIV2.

(A) An oxygen reduction assay shows that autoxidation of DMWH2, blue curve, is decreased by the presence of 500 nM bovine superoxide dismutase (SOD), orange curve. Measurement of oxygen reduction by CIII2CIV2 in the presence of bovine SOD, green curve, allows calculation of CIII2CIV2 activity. (B) Structure of CIII2CIV2 inhibitor telacebec (Q203). (C) Titration of CIII2CIV2 (60 nM) with telacebec shows an IC50 of 53 ± 19 nM (± s.d., n = 3 independent titrations) with 100 µM DMWH2.

Telacebec (Figure 2B) is a potent inhibitor of mycobacterial CIII2 (Pethe et al., 2013). The compound consists of an imidazo[1,2-a]pyridine attached via an amide linker to an N‐[(4‐{4‐[4‐(trifluoromethoxy)phenyl]piperidin‐1‐yl}phenyl)methyl] ‘tail’. Titrations of CIII2CIV2 activity with varying concentrations of telacebec (Figure 2C) show an IC50 of 53 ±19 nM (± s.d., n = 3 independent titrations, with two titrations from one batch of purified protein and a third titration from a second batch of purified protein) with 65 nM CIII2CIV2 and 100 µM DMWH2. This IC50 is lower than the 840 ± 22 nM seen with the menadiol-based assay (Gong et al., 2018), but higher than the 20 nM concentration needed to inhibit 50% of respiratory chain activity with inverted membrane vesicles from M. smegmatis (Lu et al., 2018) or 2.7 nM required to inhibit the 50% of M. tuberculosis growth in liquid culture (Pethe et al., 2013). The increased IC50 in the current assay compared to assays with inverted membrane vesicles or bacterial growth in liquid culture may be due to the binding affinity or high concentration of DMWH2, which could allow DMWH2 to compete with telacebec for binding to the complex. In addition, differences in inhibition in the different assays could be due to CIII2CIV2 being in detergent micelles rather than a lipid bilayer. The M. tuberculosis telacebec resistance mutations T313A and T313I (Pethe et al., 2013), equivalent to mutation of Thr308 in M. smegmatis, are near the QP site and suggest that the inhibitor could interfere with MQH2 binding to CIII2CIV2.

The CIII2CIV2 structure has endogenous MQ in its QP site

As telacebec is expected to bind near the QP site of CIII2CIV2, we carefully characterized this site in the cryoEM map of the enzyme in the absence of inhibitor. The QP site is near the periplasmic side of the membrane, located between heme bL and the FeS cluster (Figure 3A), and is formed by several loops and α helices from both the QcrB and QcrA subunits (Figure 3B). The arrangement of structural elements in the site is conserved from other CIIIs (Sarewicz and Osyczka, 2015). The entrance to the QP site is formed by the C and F transmembrane α helices, and the cd1 α helix that separates the periplasmic side of the QP pocket from the QcrA subunit. The ef helix and ef loop from the QcrB subunit are deeper in the QP site, as is a short section from the QcrA subunit that includes the FeS-bound His368 residue (Figure 3B). His368 from QcrA is believed to have an important role in CIII, accepting a proton during quinol oxidation at the QP site (Mulkidjanian, 2005).

Figure 3 with 1 supplement see all
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Telacebec binding to the QP site.

(A) The dashed boxes indicate the two QP sites in CIII2CIV2, each showing menaquinone (blue), the Rieske protein FeS (yellow), and heme bL (red). (B) In the inhibitor-free structure, there is density for endogenous menaquinone (pink model and gray surface) distal from the FeS group among the well-conserved structural elements of the QP site. (C) In the inhibitor-bound structure, there is density for telacebec (orange model and gray surface) deeper in the QP site where it can form numerous interactions with the protein, including possible hydrogen bonds (dashed teal lines), hydrophobic interactions (dashed green line), a halogen bond (dashed purple line), and an aromatic interaction (dashed pink line). (D) A two-dimensional (2D) representation of the interactions between telacebec and residues of CIII2CIV2 using the same color convention as in part C.

In the inhibitor-free structure there is density for endogenous MQ in the QP site (Figure 3B, pale blue surface). With the standard deviation of the cryoEM map normalized to σ = 1, the head group of MQ matches the density at 4.4σ. However, even with this strong density, the symmetry of the head group (Figure 2—figure supplement 1) makes it difficult to determine which of two poses, related by a 180° rotation, is correct. This ambiguity is exacerbated by weak density for the MQ tail, which is visible at 2.6σ, closer to the 1.7σ threshold used for visualizing lipids in the map. Figure 3B depicts the MQ pose that appears to match the density slightly better than the rotated pose, and is also the same pose as modeled previously (Gong et al., 2018). It is also possible that MQ could bind the structure in either pose, with the experimental map showing the average of both orientations.

The naphthoquinone head group of MQ is positioned near the entrance to the site, between the F and C helices (Figure 3A). This position for endogenous MQ was reported in a previous study of CIII2CIV2 from M. smegmatis (Gong et al., 2018). In this position, the naphthoquinone head group is ~14 Å away from the FeS cluster and the hydroxyl proton is ~15 Å from His368, which is too far for rapid coupled electron and proton transfer from MQH2 to FeS and His368, respectively. This distance contrasts the deeper binding position adopted by UQ in ovine CIII2 (Letts et al., 2019). It is also further from the FeS than the position observed for UQ-analogue inhibitors such as stigmatellin bound to chicken CIII2 (Zhang et al., 1998), as well as 5-undecyl-6-hydroxy-4,7-dioxobenzothiazole (Esser et al., 2004) and 2-n-nonyl-4-hydroxyquinoline N-oxide (Gao et al., 2003) bound to bovine CIII2. In the deeper position the head groups of UQ or its analogues are wedged between the ef helix/loop, and the cd1 helix, with the tail trailing between the F and C helix at the QP site entrance (Figure 3—figure supplement 1A). In these structures, the distance between the quinone head group and the FeS cluster depends on the position of the mobile Rieske head domain, but can be as little as ~7 Å, allowing for rapid electron transfer from the UQH2 to the FeS (Moser et al., 2006).

Telacebec replaces MQ in the QP site of active CIII2CIV2

Our initial attempts to image CIII2CIV2 with telacebec failed to resolve the inhibitor (Figure 3—figure supplement 1B, left), leading us to consider the possibility that inhibitor binding occurs during substrate turnover by the enzyme. CryoEM of CIII2CIV2 in the presence of DMWH2 but without telacebec confirmed that under these conditions the density in the QP site was indistinguishable from MQ seen with the enzyme at rest (Figure 3—figure supplement 1B, right). We then incubated CIII2CIV2 with both DMWH2 and telacebec to produce an inhibited complex and determined the structure of this complex to a nominal resolution of 3.0 Å by cryoEM (Figure 1—figure supplement 1, Table 1). Telacebec binding did not cause large-scale conformational changes in CIII2CIV2 but produced a clear density for the inhibitor in each of the two QP sites in the CIII2 dimer (Figure 3C). The inhibitor’s imidazopyridine moiety, amide linker region, and A-phenyl and B-piperidinyl moieties are all resolved clearly, with weaker density toward the end of the tail, which points into the lipid bilayer toward the cytoplasmic side of the membrane (Figure 3C, pale blue surface). These densities show that telacebec binds with its head group deep within the QP binding pocket in a pose similar to UQ and the UQ-analogue inhibitors bound within the canonical CIII2 as described above (Figure 3—figure supplement 1A). Telacebec’s imidazopyridine moiety forms multiple interactions with the protein to stabilize inhibitor binding. Although hydrogen bonds cannot be detected with complete confidence at the present resolution, the position of the N1 nitrogen in telacebec’s imidazopyridine moiety is consistent with formation of a hydrogen bond with the His368 from the QcrA subunit, which also binds the FeS group (Figure 3C and D, dashed teal line). The occurrence of a similar hydrogen bond between UQ and the equivalent histidine residue in canonical CIII2 (Zhang et al., 1998) has been proposed to coordinate the Q cycle (e.g. see Sarewicz and Osyczka, 2015). The 2-ethyl group from the imidazopyridine is ~4 Å away from Ile178 from the QcrB subunit, providing hydrophobic interactions (Figure 3C and D, dashed green line), while the 6-chloro group from the imidazopyridine is close to the backbone carboxyl group of Leu166 from the QcrB subunit, enabling formation of a possible halogen bond (Figure 3C and D, dashed purple line).

In addition to its imidazopyridine moiety, the amide linker and tail of telacebec also contact subunit QcrB, stabilizing binding. Thr308, which is homologous with M. tuberculosis Thr313 and is known to be important for binding (Pethe et al., 2013), is <4 Å away from the linker region of telacebec. Although the rotameric state of Thr308 is ambiguous at the current resolution, one of the rotamer states could form a stabilizing hydrogen bond with the carbonyl group of the linker region (Figure 3C and D, dashed teal line). Finally, Phe156 is ~3.5 Å from the A-phenyl group of telacebec, allowing for aromatic-aromatic interaction between the protein and inhibitor (Burley and a, 1985; Figure 3C and D, dashed pink line) Interestingly, in the inhibitor-free specimen, the MQ head group is positioned similarly to the A-phenyl ring of telacebec and may form similar stabilizing contacts with the QcrB subunit (Figure 3B and C).

Discussion

With telacebec and congeners having nanomolar inhibitory activity for both CIII2CIV2 and M. tuberculosis growth in vitro, antimycobacterial activity need not be improved for therapeutic purposes. However, the structural analysis reported here provides constraints and minimal requirements for activity of imidazopyridines and isosteric heterocycles with improved pharmacokinetic and physicochemical properties. Optimized physicochemical properties are important for drug production, including synthesis and purification. Improved pharmacokinetic properties could be enabled by design of analogues that retain target activity but are not recognized by mycobacterial efflux pumps, which are known to remove telacebec from bacterial cells to attenuate its antimycobacterial activity (Jang et al., 2017).

The structure also suggests how mutations can provide resistance to telacebec and why telacebec selectively inhibits mycobacterial CIII2. The mutation T313A in M. tuberculosis (T308A in M. smegmatis) confers resistance to telacebec (Pethe et al., 2013), likely by removing the stabilizing hydrogen bond with the carbonyl group from the linker region of the inhibitor proposed above (Figure 3C and D). M. smegmatis grown in the presence of the telacebec analogue TB47 developed the mutation H190Y (Lu et al., 2019), which is adjacent to the cd1 helix and may alter the shape of the QP binding site (Figure 3—figure supplement 1C). The selectivity of telacebec for mycobacterial CIII2CIV2 may derive, in part, from the lack of Thr308 in mammalian mitochondrial CIII2 (Figure 3—figure supplement 1D). In addition, there may be clashes between the rigid telacebec tail and both Leu150 and Ile146 in mammalian CIII2 (bovine numbering) due to the different location of the cd1 helix and the bulkier side chains in this region of the mammalian protein (Figure 3—figure supplement 1D). Interestingly, the mutation I147F (M. smegmatis numbering) results in resistance to the inhibitor stigmatellin in the Saccharomyces cerevisiae CIII2 (di Rago et al., 1989).

The telacebec-bound structure also provides insight into the basic mechanism of MQH2:O2 oxidoreductase activity by CIII2CIV2. Multiple MQ binding sites were modeled in an earlier 3.5 Å resolution cryoEM density map of CIII2CIV2, but the significance of these sites was not clear (Gong et al., 2018). The structure presented here shows that telacebec, which can inhibit MQH2:O2 oxidoreductase activity completely (Figure 2C), binds only at the QP site. Therefore, it is unlikely that MQ binding other than within the QP site is involved in electron transfer to oxygen. During the Q cycle, two molecules of MQH2 are oxidized to MQ at the QP site for each molecule of MQ reduced to MQH2 at the QN site. It is possible that MQ is channeled between the QP and QN sites by staying loosely bound to the supercomplex surface, with the additional MQ sites serving as intermediate positions along the channeling pathway. A similar model was suggested for the spinach b6f complex, which is structurally and functionally related to CIII and carries out a Q cycle in plant chloroplasts with the hydrophobic electron carrier plastiquinone (Malone et al., 2019). It is also possible that these alternative MQ sites sequester MQ, increasing the local concentration of substrate near its two binding sites, which has been seen to increase the local concentration of ligands in other systems (Vauquelin and Charlton, 2010).

Within the QP site different positions for both UQ and MQ have been described previously, both for canonical CIII and for CIII within a CIII2CIV2 supercomplex, respectively (Ding et al., 1992; Moe et al., 2021). Although the endogenous MQ found in the structure is most likely oxidized, the position it occupies is along the access path to the FeS center. We speculate that reduced MQH2 also adopts the same position, at least transiently, during turnover. This binding site within QP is denoted the Q1b position. As discussed above, the Q1b position is too far for rapid transfer of protons and electrons from MQH2 to His368 and the FeS center, respectively (Figure 4A). In contrast, movement of MQH2 deeper within the QP site to a Q1a position would bring it less than 10 Å from FeS, close enough to donate electrons to the redox center and protons to His368 (Figure 4B). The oxidized MQ could then return to the Q1b position, as observed in the present structure (Figure 4C), before it is exchanged for MQH2 from the membrane. In an emerging model for CIII2CIV2 function, the Q1b position serves as a ‘stand-by’ site for MQH2, with oxidation of the substrate occurring only upon relocation to Q1a (see Moe et al., 2021; Mulkidjanian, 2005). The structure of CIII2CIV2 with telacebec bound shows that the compound serves as dual site inhibitor (Figure 4D), with the imidazopyridine group bound to the Q1a position and A-phenyl portion of the tail bound to the Q1b position. Indeed, the possible hydrogen bond between Thr308 and the carboxyl group in the linker region of telacebec could also occur between Thr308 and MQ, stabilizing it in the Q1b position. Therefore, the observed pose of the inhibitor within the enzyme not only blocks MQ access to the FeS center but fills the QP site entirely.

Figure 4
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Model for oxidation of MQH2 in the QP site and how telacebec blocks it.

An emerging model for MQH2 reduction at the QP site proposes that the substrate binds in the Q1b position where it is too far from FeS to donate protons and electrons (A). Upon moving deeper into the QP site to the Q1a position, MQH2 is oxidized to menaquinone (MQ), donating its first electron to FeS, its second electron to heme bL, and releasing two protons to the positively charged periplasmic side of the lipid bilayer (B). Telacebec binds deep within the QP site, forming numerous interactions with the protein and blocking both the Q1a and Q1b positions (C).

Inhibiting CIII2CIV2 has demonstrated antituberculosis activity in humans (de Jager et al., 2020), even though M. tuberculosis possesses cyt. bd as an alternative enzyme that can oxidize MQH2 and sustain the electron flux to oxygen in the mycobacterial membrane. More potent killing of mycobacterial pathogens can be accomplished by simultaneous inhibition of both the CIII2CIV2 and cyt. bd terminal oxidases (Beites et al., 2019; Kalia et al., 2017; Lee et al., 2021). The present study demonstrates how cryoEM can reveal the mechanisms of electron transport chain inhibitors, enabling new strategies for targeting mycobacterial infections.

Materials and methods

Construction of M. smegmatis strain, cell culture, and protein isolation

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An M. smegmatis strain with a 3×FLAG tag at the C terminus of subunit QcrB was generated with the ORBIT method (Murphy et al., 2018). This method requires transformation of the parent strain with a plasmid encoding the Che9c phage RecT annealase and Bxb1 integrase, a payload plasmid with the desired insert, and an oligonucleotide that guides integration of the payload into the chromosomal DNA. The parent strain MC2155 was transformed with plasmid pKM444, which encodes the Che9c annealase and Bxb1 integrase. The resulting strain was subsequently transformed with payload plasmid pSAB41, which encodes a 3×FLAG tag and was described previously (Guo et al., 2021), as well as the targeting oligonucleotide 5′-CAAGTCGCTCACGGCGCTCAAGGAGCACCAGGACCGCATCCACGGCAACGGGGAGACCAACGGTCATCACGGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACCTGATCGCTGAGATACTCGGATCGCCGCAATTCCTCTTCGGAGGGGTTGCGGCGATCTTTTTATGTGCGCTTTC-3′. The resulting strain ‘M. smegmatis QcrB-3xFLAG’ was selected with hygromycin (50 μg/mL) and correct insertion of the 3×FLAG sequence was confirmed by colony PCR.

M. smegmatis was cultured in 7H9 medium (Sigma) supplemented with TDS (10 g/L tryptone, 2 g/L dextrose, 0.8 g/L NaCl). A preculture in liquid medium (15 mL) was inoculated with cells from an agar plate and grown at 30°C with shaking at 180 rpm for 48 hr. This culture was used to inoculate a larger culture (6 L), which was grown at the same conditions for a further 48 hr. Cells were harvested by centrifugation at 6900× g for 20 min and frozen in liquid nitrogen for subsequent use. After thawing, cells were broken by four passes through a continuous flow cell disruptor (Avestin) at 20 kpsi and membranes were harvested by centrifugation at 39,000× g for 30 min.

To purify CIII2CIV2, membranes were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5 mM EDTA) at 4 mL/g and solid dodecyl maltoside (DDM) detergent was added to 1% (w/v) with stirring at 4°C for 45 min. Insoluble material was removed by centrifugation at 149,000× g for 45 min and the solubilized protein was loaded onto a gravity column of 2 mL M2 anti-FLAG affinity matrix (Sigma). The column was washed with 10 mL of wash buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.02% [w/v] DDM) and eluted with 5 mL of wash buffer supplemented with 3×FLAG peptide at 150 μg/mL. Purified protein was exchanged into 50 mM Tris-HCl pH 7.4, 150 mM NaCl, and 0.003% (w/v) glycol-diosgenin (GDN) with a 100 kDa molecular weight cutoff concentrator (Sigma).

Activity assays

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DMW (Enamine) at 20 mM in anhydrous ethanol (400 μL) on ice was reduced with a few grains of NaBH4 and the reaction was quenched by addition of 4 N HCl (10–20 μL). Enzymatically reduced DMWH2 was prepared with 7.5 mM NADH, 200 DMW, and 300 μg/mL NDH-2 from Caldalkalibacillus thermarum (Nakatani et al., 2017). Oxygen-reduction assays were performed with an Oxygraph Clark-type electrode (Hansatech) in 1 mL of reaction buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5 mM EDTA, and 500 nM bovine SOD [Sigma]). CIII2CIV2 was added (65 nM) and reactions were initiated by addition of 100 µM DMWH2. For inhibition studies, telacebec (DC Chemicals) at varying concentrations was incubated with 65 nM CIII2CIV2 in the reaction buffer for 3 hr at 4°C. This mixture was added to the Oxygraph and reactions were initiated by addition of 100 µM DMWH2. To account for any background oxygen reduction that still occurs in the presence of SOD, the rate of oxygen reduction in the presence of DMWH2 and SOD, but in the absence of CIII2CIV2, was subtracted from the rate in the presence of DMWH2, SOD, and CIII2CIV2. The resulting oxygen reduction rates for CIII2CIV2 at different concentrations of telacebec were fit with a Python script. Individual inhibition curves, which were produced on different days with different preparations of reagent, were fit individually, with the average of the IC50 values reported and the standard deviation of the fitted IC50 values reported as the error (Dahlin et al., 2004). Plots were produced using the Python matplotlib library.

CryoEM specimen preparation and imaging

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For cryoEM of inhibitor-free CIII2CIV2, enzyme at ~16 mg/mL (2 µL) was applied to homemade nanofabricated holey gold grids (Marr et al., 2014), which had previously been glow-discharged in air for 120 s at 20 mA (PELCO easiGlow), within a Vitrobot Mark III (FEI) at 4°C and 100% relative humidity. Grids were blotted for 24 s before freezing. For cryoEM of telacebec-bound CIII2CIV2, DMWH2 in ethanol was added to 100 µM (0.02% ethanol) and telacebec in DMSO was added to 25 µM (1.5% DMSO) to a solution containing purified CIII2CIV2 at ~0.08 mg/mL (6 mL). The solution was concentrated ~100-fold by centrifugation at 700× g with a 100 kDa molecular weight cutoff centrifuged concentrator device (Sigma). The sample (2 µL) was then applied to homemade nanofabricated holey gold grids, which had previously been glow-discharged in air for 120 s at 20 mA, within an EM GP2 (Leica) grid freezing device at 4°C and 100% relative humidity. Grids were blotted for 1 s before freezing.

Screening of specimens was done with an FEI Tecnai F20 electron microscope equipped with a K2 Summit direct detector device camera. High-resolution cryoEM data were collected with a Titan Krios G3 electron microscope (Thermo Fisher Scientific) operated at 300 kV and equipped with a Falcon 4 direct detector device camera. Automated data collection was done with the EPU software package. The inhibitor-free dataset consisted of 2793 movies and telacebec-bound sample consisted of 4308 movies. Movies were collected at a nominal magnification of 75,000× with a calibrated pixel size of 1.03 Å. Movies consisted of 30 exposure fractions over 7.7 s. The camera exposure rate and the total exposure were 5.99 e-/pixel/s and ~43.5 e-/Å2, respectively (Table 1).

Image analysis and atomic model building

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All image analysis was performed within the cryoSPARC software package, version 3 (Punjani et al., 2017), including individual particle motion correction (Rubinstein and Brubaker, 2015), non-uniform refinement (Punjani et al., 2020), and 3DVA (Punjani and Fleet, 2021). Image analysis and 3D reconstruction for each dataset was performed in the same way. Motion was corrected and CTF parameters were estimated for each movie in patches. Manual particle selection and 2D classification was used to generate templates, which were in turn used to select of 387,777 and 1,037,709 particle images for the telacebec-bound and inhibitor-free datasets, respectively. Datasets were cleaned with 2D classification, 3D classification, and heterogeneous refinement to 70,818 and 150,885 particle images for the telacebec-bound and inhibitor-free datasets, respectively. Beam tilt was corrected and each map was refined with non-uniform refinement without symmetry enforced. CTF values were then refined, the detergent micelle subtracted, and alignment parameters adjusted with local refinement with C2 symmetry enforced, yielding maps at 3.0 Å resolution for each dataset. 3DVA was done on a pooled dataset with masks including the SOD subunit or cyt. cc domain. Atomic models were constructed starting from previous models of the complex (Gong et al., 2018; Wiseman et al., 2018). Additions to the models were made in Coot (Emsley et al., 2010) and refined with Phenix (Liebschner et al., 2019) and ISOLDE (Croll, 2018).

Structure-activity relation analysis studies

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Insight into protein-inhibitor interaction was facilitated by analysis with the Schrödinger software package (Release 2019–1). The protein preparation wizard within Schrödinger was used to prepare the protein for modeling. Briefly, the QcrA and QcrB chains and the ligand from the PDB file were merged and pre-processed to add missing hydrogen atoms, fill in missing side chains, and adjust ionization and tautomeric states of the ligand. The hydrogen bond network between the protein’s amino acids and the ligand was optimized by allowing reorientation of amino acid side chains like His, Asn, Asp, Glu, and Gln, and the ionization and tautomeric states of these side chains were estimated (Olsson et al., 2011). The resulting structure was refined to remove clashes and optimize geometry with the OPLS3e force field (Roos et al., 2019). These changes did not noticeably affect the fit of the model within the experimental cryoEM density map.

Data availability

Data deposition: all electron cryomicroscopy maps described in this article have been deposited in the Electron Microscopy Data Bank (EMDB) (accession numbers EMD-24455 to EMD-24457) and atomic models have been deposited in the Protein Database (PDB) (accession numbers 7RH5 to 7RH7).

The following data sets were generated
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) Electron Microscopy Data Bank
    ID EMD-24455. Mycobacterial CIII2CIV2 supercomplex, Inhibitor free.
    https://www.ebi.ac.uk/emdb/EMD-24455
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) Electron Microscopy Data Bank
    ID EMD-24456. Mycobacterial CIII2CIV2 supercomplex, inhibitor free, -Lpqe cyt cc open.
    https://www.ebi.ac.uk/emdb/EMD-24456
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) Electron Microscopy Data Bank
    ID EMD-24457. Mycobacterial CIII2CIV2 supercomplex, Telacebec (Q203) bound.
    https://www.ebi.ac.uk/emdb/EMD-24457
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) RCSB Protein Data Bank
    ID 7RH5. Mycobacterial CIII2CIV2 supercomplex, Inhibitor free.
    https://www.rcsb.org/structure/7RH5
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) RCSB Protein Data Bank
    ID 7RH6. Mycobacterial CIII2CIV2 supercomplex, inhibitor free, -Lpqe cyt cc open.
    https://www.rcsb.org/structure/7RH6
    1. Di Trani JM
    2. Yanofsky DJ
    3. Rubinstein JL
    (2021) RCSB Protein Data Bank
    ID 7RH7. Mycobacterial CIII2CIV2 supercomplex, Telacebec (Q203) bound.
    https://www.rcsb.org/structure/7RH7

References

    1. Dahlin JL
    2. Sittampalam GS
    3. Coussens NP
    4. Devanarayan V
    5. Weidner JR
    6. Iversen PW
    7. Haas J
    8. Bronson DD
    9. Trask OJ
    10. Wiernicki TR
    11. Kahl SD
    (2004)
    Basic guidelines for reporting non-clinical data
    Assay Guid Man 1:47.
    1. Fieser LF
    2. Fieser M
    (1934)
    The reduction potentials of various naphthoquinones
    Journal of the American Chemical Society 57:491–494.
  56. Book
    1. World Health Organization
    (2019)
    The Selection and Use of Essential Medicines
    Geneva: World Health Organization.

Decision letter

  1. Andrew P Carter
    Reviewing Editor; MRC Laboratory of Molecular Biology, United Kingdom
  2. Olga Boudker
    Senior Editor; Weill Cornell Medicine, United States
  3. James A Letts
    Reviewer; University of California Davis, United States

Our editorial process produces two outputs: i) public reviews designed to be posted alongside the preprint for the benefit of readers; ii) feedback on the manuscript for the authors, including requests for revisions, shown below. We also include an acceptance summary that explains what the editors found interesting or important about the work.

Acceptance summary:

The paper by Rubinstein and colleagues is the first study to elucidate the atomic-resolution structure of the major respiratory complex of Mycobacterium tuberculosis inhibited by Telacebec (Q203), a novel first-in-class proven antituberculosis drug currently in phase II clinical trials. The work provides a new molecular blue print for improving and developing more inhibitors of this essential respiratory complex in the fight against the global pandemic of drug resistant tuberculosis disease.

Decision letter after peer review:

Thank you for submitting your article "Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)" for consideration by eLife. Your article has been reviewed by 3 peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Olga Boudker as the Senior Editor. The following individual involved in review of your submission has agreed to reveal their identity: James A Letts (Reviewer #1).

The reviewers have discussed their reviews with one another, and the Reviewing Editor has drafted this to help you prepare a revised submission.

Essential revisions:

1) Please correct geometry violations in the some of your lipids – see Reviewer 1 for details.

2) Ensure activity assays are performed with biological replicates (separate protein preparation etc). – see Reviewer 2 for details.

Reviewer #1 (Recommendations for the authors):

The discussion of the new activity assay conditions could be strengthened by a Figure supplement comparing the structures of menaquinone, menadiol and 2,3-dimethyl-[1,4]naphthpquinone. The relative importance of the switch from menadiol to 2,3-dimethyl-[1,4]naphthpquinone vs. the addition of SOD to the reaction conditions is unclear. Does the addition of SOD to the menadiol assay result in a similar reduction of autoxidation? If known, the proposed mechanism by which SOD slows autoxidation should also be briefly mentioned as this activity is key to the improved assay and interpretation of the results.

Videos from the 3D variability analysis showing the movement of the SOD subunit and loss of the LpqE subunit would aid this section.

Page 6, paragraph 2 the authors state "The increased IC50 in the current assay compared to assays with inverted membrane vesicles or bacterial growth in liquid culture may be due to the binding affinity or high concentration of DMW, which could allow DMW to compete with telacebec for binding to the complex." Are the authors able to use their assay to determine the KM for DMW? Have they attempted IC50 measurements at different DMW concentrations? Is the mode of telacebec inhibition well characterized? How would the differences between detergent and membrane environments impact the measured IC50?

On page 6, paragraph 4, sentence 1, the authors reference "strong density for the endogenous MQ", the term "strong density" is ambiguous and should be quantified. If the map is normalized, at what σ value is the density clearly visible? How does this σ value compare to those of other map features, such as heavy metals, core side chains, other lipids or the detergent micelle? When looking at the map it is difficult to trace many of the modeled lipid and isoprenoid tails in the density. Also the orientation of the menaquinone head group in the QP site appears ambiguous, the isoprenoid tail could be as modeled or could go towards Met189 with the menaquinone head flipped 180 degrees. Why did the authors choose the current orientation? Given the symmetry of the menaquinone head are both binding orientations possibly present and averaged out in the structure? Would this help explain the ambiguity in the density for the isoprenoid chain?

There are many geometry violations in the isoprenoid chains of the modeled menaquinones (see chain F MQ9 607 C34 for an extreme example), the authors should double check their ligand restraints file. Overall the density for chain F MQ9 607 is unconvincing that it belongs to a menaquinone. The density for the isoprenoid tail for chain F MQ9 609 is very weak and difficult to follow why it was traced in the way it was (regions of it are not in density, whereas significant density in the same vicinity remains unmodeled). This issue is also seen in other regions of the map/model, i.e. there are several clear densities for lipids that are not modeled and modeled acyl chains that are not in clear density. The authors should carefully reevaluate their lipid and menaquinone modeling before publication.

The authors first mention His368 on page 6 but do not discuss its significance until pages 7 and 8. Clarity could be improved by discussing the significance of His368 when it is first mentioned.

Given that the co-purified menaquinone seen in the inhibitor-free enzyme is likely oxidized, wouldn't the Q1b position more likely be for oxidized menaquinone as opposed to the reduced menaquinol as depicted in Figure 4A? If so wouldn't the menaquinone in the Q1b position likely be more representative "exiting" the QP-site after donating its electrons, as opposed to a menaquinol "entering" the QP-site in order to donate its electrons? If so, what would the implications be for the reversibility of the Q-cycle and semiquinone formation at the QP-site, to have a menaquinone binding site adjacent to the QP site that is too distance to donate electrons to or accept electrons from the FeS cluster or heme bL?

Reviewer #2 (Recommendations for the authors):

My only recommendation is using multiple biological replicates (at least two, better three) for the activity assays.

Reviewer #3 (Recommendations for the authors):

1. Readers will be interested to see how reported mutations in qcrB in M. tuberculosis that confer resistance to Q203 map onto the CIII2CIV2 inhibited complex. How does the H190Y mutation reported for TB47 map to the new structural data with the Q203-inhibited structure (see Lu et al., ACS Infectious Diseases 5:239-249 (2019) PMID: 30485737).

2. A short paragraph might be appropriate on how the new structural data reported herein could be used to improve on inhibitors of this supercomplex.

3. A structural evaluation of why Q203 is unable to inhibit the mitochondrial Complex III structure could be included.

4. The Beites et al., 2019 reference should also include these two citations:

Lee BS, et al., EMBO Molecular Medicine 13: e13207 (2021)

Kalia, NP, et al., Proceedings of the National Academy of Sciences of the United States of America 114:7426-7431 (2017)

https://doi.org/10.7554/eLife.71959.sa1

Author response

Essential revisions:

1) Please correct geometry violations in the some of your lipids – see Reviewer 1 for details.

2) Ensure activity assays are performed with biological replicates (separate protein preparation etc). – see Reviewer 2 for details.

Reviewer #1 (Recommendations for the authors):

The discussion of the new activity assay conditions could be strengthened by a Figure supplement comparing the structures of menaquinone, menadiol and 2,3-dimethyl-[1,4]naphthpquinone. The relative importance of the switch from menadiol to 2,3-dimethyl-[1,4]naphthpquinone vs. the addition of SOD to the reaction conditions is unclear. Does the addition of SOD to the menadiol assay result in a similar reduction of autoxidation?

We thank the reviewer for this suggestion. Three figure supplement panels have been added (Figure 2 —figure supplement 1A-C) to show the structural differences between the different CIII2CIV2 substrates.

We had initially selected DMWH2 over menadiol based on DMWH2 having a more favorable redox potential and literature that states that menaquinol autoxidizes faster than DMWH2. The reviewer’s comment prompted us to compare menaquinol and DMWH2 experimentally and investigate the effect of SOD on autoxidation of menaquinol. We found that in our conditions, menaquinol autoxidizes more slowly than DMWH2 and the autoxidation can be limited by SOD, but even more importantly we confirmed in multiple experiments that menaquinol is not as effective as an electron donor for CIII2CIV2 as DMWH2. We have updated the relevant text (p. 5, 3rd paragraph):

“The midpoint potentials of the redox centers in mycobacterial CIII2 are lower than those of canonical mitochondrial CIII2 (Kao et al., 2016), and as a result UQH2 analogues typically used in CIII2 assays are not able to reduce CIII2 of the M. smegmatis supercomplex. MQH2 analogues (Figure 2 —figure supplement 1A-C) capable of reducing CIII2CIV2 suffer from autoxidation at neutral or basic pH, which leads to oxygen reduction even in the absence of enzyme (Munday, 2000). This background oxygen-reduction rate is typically subtracted from oxygen reduction observed in the presence of enzyme to calculate the enzyme-catalyzed oxidoreductase activity. Previous measurement of CIII2CIV2 activity employed 2-methyl-[1,4]naphthohydroquinone (menadiol) (Gong et al., 2018) or 2,3-dimethyl-[1,4]naphthohydroquinone (DMWH2) (Graf et al., 2016; Wiseman et al., 2018) as the electron donor. Although both substrates are susceptible to autoxidation, the rate of autoxidation was proposed to be ~30% slower for DMWH2 compared to menadiol at the pH 7.5 of our oxygen consumption assays (Munday, 2000). In contrast to these earlier studies, we found that menaquinol autoxidized more slowly than DMWH2 (Figure 2 —figure supplement 1D). However, we also found that mendiol was substantially less efficient than DMWH2 as an electron donor for CIII2CIV2, likely due its less favorable redox potential (Fieser and Fieser, 1934), supporting the choice of DMWH2 as the substrate in assays (Figure 2 —figure supplement 1D).”

We have added a panel to Figure 2 – Supplement 1 that compares the behaviour of menadiol and DMWH2.

As seen in Figure 2 – Supplement 1, SOD does limit autoxidation of menaquinol (but does not solve the problem of menaquinol being a poor electron donor to CIII2CIV2). To make this point clear we have added the following text (p.5, 4th paragraph):

“To remove error introduced by the effect of the SOD subunit on the observed oxygen reduction signal, we established that bovine C-type SOD can similarly limit the autoxidation of DMWH2 (Figure 2A, blue and orange curves), as well as menadiol (Figure 2 —figure supplement 1D).”

If known, the proposed mechanism by which SOD slows autoxidation should also be briefly mentioned as this activity is key to the improved assay and interpretation of the results.

The autoxidation of quinols is complex process, thought to involve as many as seven different steps in the reaction. A model for how SOD slows autoxidation has been proposed and we now provide a concise description of the process, along with a citation to the reference where it is described more fully (p. 5, 4th paragraph):

“Autoxidation of quinols is believed to involve a superoxide anion intermediate, with the SOD-catalyzed dismutation of the intermediate to hydrogen peroxide removing this reactant to slow the process (Munday, 2000).”

Videos from the 3D variability analysis showing the movement of the SOD subunit and loss of the LpqE subunit would aid this section.

We agree with the reviewer and have now added the following supplementary videos and referred to them in the text where 3D variability is described:

Video 1. Three-dimensional variability analysis of CIII2CIV2 showing the presence of LpqE with the cyt. cc subunit in the closed position or the absence of LpqE with the cyt. cc subunit in the open position. Subunits are coloured as in Figure 1. Please view as loop.

Video 2. Three-dimensional variability analysis showing movement of SOD subunit of CIII2CIV2. Subunits are coloured as in Figure 1. Please view as loop.

Page 6, paragraph 2 the authors state "The increased IC50 in the current assay compared to assays with inverted membrane vesicles or bacterial growth in liquid culture may be due to the binding affinity or high concentration of DMW, which could allow DMW to compete with telacebec for binding to the complex." Are the authors able to use their assay to determine the KM for DMW? Have they attempted IC50 measurements at different DMW concentrations? Is the mode of telacebec inhibition well characterized? How would the differences between detergent and membrane environments impact the measured IC50?

Unfortunately, we are not able to reliably measure the KM for DMW due to technical limitations with the oxygen consumption assay. First, the assays are extremely low throughput, with each measurement done separately and requiring extensive measurement time and a large quantity of purified protein. Further, measuring the activity at lower DMW concentrations is both technically and practically prohibitivly challenging. Even though the autoxidation rate is decreased in the presence of SOD, it is not eliminated completely (see Figure 2A and Figure 2 —figure supplement 1). Lowering the DMW concentrations decreases the measured CIII2CIV2 activity such that it approaches the autoxidation rate, which introduces large errors into the determination of the activity. Furthermore, because each activity measurement requires monitoring the O2-reduction rate for tens of seconds, the simultaneous formation of oxidized DMW by autoxidation results in accumulation of oxidized DMW that, at low DMW concentrations, yields non-linear O2-oxidation kinetics and prevents reliable determination of the slope of the O2 concentration curve. While we agree that a highly detailed analysis of telacebec inhibition of CIII2CIV2 at varying conditions would be desirable, we consider measuring a reliable IC50 at even one condition an important advance.

The reviewer raises a good point that differences in IC50 can also be due to measuring activity in detergent micelles rather than lipid bilayers. Therefore, we have added the following text to introduce this possibility (p. 6, 2rd paragraph):

“In addition, differences in inhibition in the different assays could be due to CIII2CIV2 being in detergent micelles rather than a lipid bilayer.”

On page 6, paragraph 4, sentence 1, the authors reference "strong density for the endogenous MQ", the term "strong density" is ambiguous and should be quantified. If the map is normalized, at what σ value is the density clearly visible? How does this σ value compare to those of other map features, such as heavy metals, core side chains, other lipids or the detergent micelle? When looking at the map it is difficult to trace many of the modeled lipid and isoprenoid tails in the density. Also the orientation of the menaquinone head group in the QP site appears ambiguous, the isoprenoid tail could be as modeled or could go towards Met189 with the menaquinone head flipped 180 degrees. Why did the authors choose the current orientation? Given the symmetry of the menaquinone head are both binding orientations possibly present and averaged out in the structure? Would this help explain the ambiguity in the density for the isoprenoid chain?

We thank the reviewer for these suggestions. We have modified the text as follows to address them (p.7, 1st paragraph):

“In the inhibitor-free structure there is density for endogenous MQ in the QP site (Figure 3B, pale blue surface). With the standard deviation of the cryoEM map normalized to σ=1, the head group of MQ matches the density at 4.4σ. However, even with this strong density, the symmetry of the head group (Figure 2 —figure supplement 1) makes it difficult to determine which of two poses, related by a 180º rotation, is correct. This ambiguity is exacerbated by weak density for the MQ tail, which is visible at 2.6σ, closer to the 1.7σ threshold used for visualizing lipids in the map. Figure 3B depicts the MQ pose that appears to match the density slightly better than the rotated pose, and is also the same pose as modelled previously (Gong et al., 2018). It is also possible that MQ could bind the structure in either pose, with the experimental map showing the average of both orientations.”

This paragraph makes clear that there is still some ambiguity in the pose of the endogeneous MQ. However, please note that the choice of pose for MQ does not affect any of the arguments made in the manuscript.

There are many geometry violations in the isoprenoid chains of the modeled menaquinones (see chain F MQ9 607 C34 for an extreme example), the authors should double check their ligand restraints file. Overall the density for chain F MQ9 607 is unconvincing that it belongs to a menaquinone. The density for the isoprenoid tail for chain F MQ9 609 is very weak and difficult to follow why it was traced in the way it was (regions of it are not in density, whereas significant density in the same vicinity remains unmodeled). This issue is also seen in other regions of the map/model, i.e. there are several clear densities for lipids that are not modeled and modeled acyl chains that are not in clear density. The authors should carefully reevaluate their lipid and menaquinone modeling before publication.

We thank the reviewer for their attention to detail and apologize for this oversight. Many of the lipids and MQ models were “inherited” from the model from Gong et al., which we used to start building our model, as described in the methods section, and these poorly modelled features were not subjected to sufficient scrutiny. MQ9 609 has now been removed and the isoprenoid tail of MQ9 607 has been altered to fit the density originally occupied by the isoprenoid tail of MQ9 609. Lipids throughout the complex (9XX 302, 9XX 204, 9Y0 201, 9YF 504, and 9YF 501) have been altered to better fit the density and the models have been reprocessed in phenix with new restraints (new report included).

The authors first mention His368 on page 6 but do not discuss its significance until pages 7 and 8. Clarity could be improved by discussing the significance of His368 when it is first mentioned.

We thank the reviewer for this suggestion. To ‘prime’ the reader for the proposed role of His368, we have added the following sentence to immediately after where His368 is mentioned for the first time (p. 6, 4th paragraph):

“His368 from QcrA is believed to have an important role in CIII, accepting a proton during quinone oxidation at the QP site (Mulkidjanian, 2005).”

His368 is then mentioned again on the 2nd paragraph of page 7:

“In this position, the naphthoquinone head group is ~14 Å away from the FeS cluster and the hydroxyl proton is ~15 Å from His368, which is too far for rapid coupled electron and proton transfer from MQH2 to FeS and His368, respectively.”

With the main discussion of His368 at the end of page 7 and beginning of page 8.

Given that the co-purified menaquinone seen in the inhibitor-free enzyme is likely oxidized, wouldn't the Q1b position more likely be for oxidized menaquinone as opposed to the reduced menaquinol as depicted in Figure 4A? If so wouldn't the menaquinone in the Q1b position likely be more representative "exiting" the QP-site after donating its electrons, as opposed to a menaquinol "entering" the QP-site in order to donate its electrons? If so, what would the implications be for the reversibility of the Q-cycle and semiquinone formation at the QP-site, to have a menaquinone binding site adjacent to the QP site that is too distance to donate electrons to or accept electrons from the FeS cluster or heme bL?

We thank the reviewer for this excellent suggestion. We agree that the co-purified endogenous menaquinone is most likely oxidized. Hence, the cryoEM structure almost certainly shows the position of the oxidized menaquinone. The Q1b position is along the only entry/exit path to/from the Q1a position where menaquinol oxidation can take place. Therefore, we speculate that during turnover, when the menaquinone pool is mostly reduced, MQH2 would have to access the Q1b position, at least transiently, because this position is found along the MQH2 transfer trajectory. We have updated the text and figure to show the model where reduced MQH­2 accesses the Q1b position, moves to the Q1a position to transfer its electron to the FeS group, and then returns to the Q1b position (p. 9, 2nd paragraph):

“Although the endogenous MQ found in the structure is most likely oxidized, the position it occupies is along the access path to the FeS center. We speculate that reduced MQH2 also adopts the same position, at least transiently, during turnover. This binding site within QP is known asthe Q1b position. As discussed above, the Q1b position is too far for rapid transfer of protons and electrons from MQH2 to His368 and the FeS center, respectively (Figure 4A). In contrast, movement of MQH2 deeper within the QP site to the Q1a position would bring it less than 10 Å from FeS, close enough to donate electrons to the redox center and protons to His368 (Figure 4B). The oxidized MQ could then return to the Q1b position, as observed in the present structure (Figure 4C), before it is exchanged for MQH2 from the membrane. In an emerging model for CIII2CIV2 function, the Q1b position serves as a "stand-by" site for MQH2, with oxidation of the substrate occurring only upon relocation to Q1a (see (Moe et al., 2021; Mulkidjanian, 2005)). The structure of CIII2CIV2 with telacebec bound shows that the compound serves as dual site inhibitor (Figure 4D), with the imidazopyridine group bound to the Q1a position and A-phenyl portion of the tail bound to the Q1b position. Indeed, the possible hydrogen bond between Thr308 and the carboxyl group in the linker region of telacebec could also occur between Thr308 and MQ, stabilizing it in the Q1b position. Therefore, the observed pose of the inhibitor within the enzyme not only blocks MQ access to the FeS center but fills the QP site entirely.”

We have updated Figure 4 to show both the reduced and oxidized forms of MQ accessing the Q1b position.

Reviewer #2 (Recommendations for the authors):

My only recommendation is using multiple biological replicates (at least two, better three) for the activity assays.

We apologize for not making clear that the measurement were indeed from multiple samples of purified proteins. However, it is important to note that the repeated assays are not simple technical replicates (e.g. multiple instrumental measurements made on the same sample). Each oxygraph measurement is done one-at-a-time in series, requiring creation of a new assay mixture with multiple reagents, reassembly of the reaction chamber, equilibration, and independent measurements of background oxygen consumption rates. Thus, protein batch is not the only significant source of variance in assays, particularly in inhibitor titrations where activity is expressed as a fraction of the uninhibited enzyme.

We have modified the relevant text (p. 6, 1st paragraph):

“With 500 nM SOD added, CIII2CIV2’s DMWH2:O2 oxidoreductase activity was measured at 91 ±4 e-/s (± s.d., n=6 independent assays with three each from two separate batches of protein), which is nearly an order of magnitude greater than the apparent activity found previously (Wiseman et al., 2018).”

And (p. 6, 2nd paragraph)

“Titrations of CIII2CIV2 activity with varying concentrations of telacebec (Figure 2C) show an IC50 of 53 nM ±19 (± s.d., n=3 independent titrations, with two titrations from one batch of purified protein and a third titration from a second batch of purified protein) with 65 nM CIII2CIV2 and 100 µM DMW.”

Reviewer #3 (Recommendations for the authors):

Specific points to address:

1. Readers will be interested to see how reported mutations in qcrB in M. tuberculosis that confer resistance to Q203 map onto the CIII2CIV2 inhibited complex. How does the H190Y mutation reported for TB47 map to the new structural data with the Q203-inhibited structure (see Lu et al., ACS Infectious Diseases 5:239-249 (2019) PMID: 30485737).

We have added the requested information to the paragraph following the description of the telecebec binding pose as well as an additional panel in the Figure 3 —figure supplement 1 (p, 8, 4th paragraph):

“The structure also suggests how mutations can provide resistance to telacebec and why telacebec selectively inhibits mycobacterial CIII2. The mutation T313A in M. tuberculosis (T308A in M. smegmatis) confers resistance to telacebec (Pethe et al., 2013), likely by removing the stabilizing hydrogen bond with the carbonyl group from the linker region of the inhibitor proposed above (Figure 3C and D). M. smegmatis grown in the presence of the telacebec analogue TB47 developed the mutation H190Y (Lu et al., 2019), which is adjacent to the cd1 helix and may alter the shape of the QP binding site (Figure 3 —figure supplement 1C).”

2. A short paragraph might be appropriate on how the new structural data reported herein could be used to improve on inhibitors of this supercomplex.

This information has now been added into the paragraph following the description of the telacebec binding pose (p. 8, 3rd paragraph):

“With telacebec and congeners having nanomolar inhibitory activity for both CIII2CIV2 and M. tuberculosis growth in vitro, antimycobacterial activity need not be improved for therapeutic purposes. However, the structural analysis reported here provides constraints and minimal requirements for activity of imidazopyridines and isosteric heterocycles with improved pharmacokinetic and physicochemical properties. Optimized physicochemical properties are important for drug production, including synthesis and purification. Improved pharmacokinetic properties could be enabled by design of analogues that retain target activity but are not recognized by mycobacterial efflux pumps, which are known to remove telacebec from bacterial cells to attenuate its antimycobacterial activity (Jang et al., 2017).”

3. A structural evaluation of why Q203 is unable to inhibit the mitochondrial Complex III structure could be included.

We have added the following analysis (p. 8, 4th paragraph):

“The structure also suggests how mutations can provide resistance to telacebec and why telacebec selectively inhibits mycobacterial CIII2.”

“The selectivity of telacebec for mycobacterial CIII2CIV2 may derive, in part, from the lack of Thr308 in mammalian mitochondrial CIII2 (Figure 3 —figure supplement 1D). In addition, there may be clashes between the rigid telacebec tail and both Leu150 and Ile146 in mammalian CIII2 (bovine numbering) due to the different location of the cd1 helix and the bulkier side chains in this region of the mammalian protein (Figure 3 —figure supplement 1D). Interestingly, the mutation I147F (M. smegmatis numbering) results in resistance to the inhibitor stigmatellin in the Saccharomyces cerevisiae CIII2 (di Rago et al., 1989)”

4. The Beites et al., 2019 reference should also include these two citations:

Lee BS, et al., EMBO Molecular Medicine 13: e13207 (2021)

Kalia, NP, et al., Proceedings of the National Academy of Sciences of the United States of America 114:7426-7431 (2017)

We thank the reviewer for pointing out these references and have added them in the final paragraph where we cite Beites et al., (2019) regarding dual inhibition of cyt. bd and cyt. bcc-aa3.

https://doi.org/10.7554/eLife.71959.sa2

Article and author information

Author details

  1. David J Yanofsky

    1. Molecular Medicine Program, The Hospital for Sick Children, Toronto, Canada
    2. Department of Medical Biophysics, The University of Toronto, Toronto, Canada
    Contribution
    Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editing
    Contributed equally with
    Justin M Di Trani
    Competing interests
    No competing interests declared

  2. Justin M Di Trani

    Molecular Medicine Program, The Hospital for Sick Children, Toronto, Canada
    Contribution
    Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editing
    Contributed equally with
    David J Yanofsky
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4764-4009

  3. Sylwia Król

    Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared

  4. Rana Abdelaziz

    Department of Pharmaceutical/Medicinal Chemistry and Clinical Pharmacy, Martin-Luther-Universitaet Halle-Wittenberg, Halle (Saale), Germany
    Contribution
    Formal analysis, Investigation, Methodology, Visualization
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9581-604X

  5. Stephanie A Bueler

    Molecular Medicine Program, The Hospital for Sick Children, Toronto, Canada
    Contribution
    Investigation, Methodology
    Competing interests
    No competing interests declared

  6. Peter Imming

    Department of Pharmaceutical/Medicinal Chemistry and Clinical Pharmacy, Martin-Luther-Universitaet Halle-Wittenberg, Halle (Saale), Germany
    Contribution
    Formal analysis, Funding acquisition, Methodology, Supervision, Writing – review and editing
    For correspondence
    peter.imming@pharmazie.uni-halle.de
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2178-3887

  7. Peter Brzezinski

    Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    Contribution
    Conceptualization, Formal analysis, Funding acquisition, Methodology, Supervision, Writing – review and editing
    For correspondence
    peterb@dbb.su.se
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3860-4988

  8. John L Rubinstein

    1. Molecular Medicine Program, The Hospital for Sick Children, Toronto, Canada
    2. Department of Medical Biophysics, The University of Toronto, Toronto, Canada
    3. Department of Biochemistry, The University of Toronto, Toronto, Canada
    Contribution
    Conceptualization, Formal analysis, Funding acquisition, Methodology, Supervision, Writing – original draft, Writing – review and editing
    For correspondence
    john.rubinstein@utoronto.ca
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0566-2209

Funding

Canadian Institutes of Health Research (PJT162186)

  • John L Rubinstein

Wallenberg Foundation (2019.0043)

  • Peter Brzezinski

Swedish Research Council (2018-04619)

  • Peter Brzezinski

Canadian Institutes of Health Research (PGS-M)

  • David J Yanofsy

Canadian Institutes of Health Research (PDF)

  • Justin M Di Trani

Canada Research Chairs

  • John L Rubinstein

Canada Foundation for Innovation

  • John L Rubinstein

Ontario Research Foundation

  • John L Rubinstein

University of Toronto

  • David J Yanofsy

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

DJY was supported by a Canada Graduate Scholarship from the Canadian Institutes of Health Research (CIHR) and a Queen Elizabeth II Graduate Scholarship in Science and Technology from the University of Toronto Department of Medical Biophysics, JMDT was supported by a postdoctoral fellowship from the Canadian Institutes of Health Research, and JLR was supported by the Canada Research Chairs program. This research was supported by CIHR grant PJT162186 (JLR), The Alice and Knut Wallenberg Foundation grant 2019.0043 (PB), and Swedish Research Council grant 2018–04619 (PB). CryoEM data were collected at the Toronto High-Resolution High-Throughput Cryo-EM facility, supported by the Canada Foundation for Innovation and Ontario Research Fund.

Senior Editor

  1. Olga Boudker, Weill Cornell Medicine, United States

Reviewing Editor

  1. Andrew P Carter, MRC Laboratory of Molecular Biology, United Kingdom

Reviewer

  1. James A Letts, University of California Davis, United States

Version history

  1. Preprint posted: July 6, 2021 (view preprint)
  2. Received: July 6, 2021
  3. Accepted: September 29, 2021
  4. Accepted Manuscript published: September 30, 2021 (version 1)
  5. Accepted Manuscript updated: October 5, 2021 (version 2)
  6. Version of Record published: October 18, 2021 (version 3)

Copyright

© 2021, Yanofsky et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. David J Yanofsky
  2. Justin M Di Trani
  3. Sylwia Król
  4. Rana Abdelaziz
  5. Stephanie A Bueler
  6. Peter Imming
  7. Peter Brzezinski
  8. John L Rubinstein
(2021)
Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)
eLife 10:e71959.
https://doi.org/10.7554/eLife.71959

Categories and tags

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