445 results found
    1. Structural Biology and Molecular Biophysics

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    1. Structural Biology and Molecular Biophysics

    All-atom molecular dynamics of the HBV capsid reveals insights into biological function and cryo-EM resolution limits

    Jodi A Hadden et al.
    All-atom molecular dynamics of the HBV capsid supports a role for structural asymmetry in biological function, reveals the potential for triangular pores to mediate cellular signaling, and indicates that capsid flexibility may limit resolution attainable by cryo-EM.
    1. Structural Biology and Molecular Biophysics

    Electron cryo-microscopy structure of the canonical TRPC4 ion channel

    Deivanayagabarathy Vinayagam et al.
    Structural insights into the architecture and functional properties of zebrafish TRPC4 are revealed by high-resolution cryo-EM.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae

    André Heuer et al.
    Near atomic resolution of late 40S ribosomal subunit assembly by cryo-EM reveals immature rRNA active sites stabilized by biogenesis factors.
    1. Structural Biology and Molecular Biophysics

    Using the Volta phase plate with defocus for cryo-EM single particle analysis

    Radostin Danev et al.
    Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling

    C Keith Cassidy et al.
    An atomic model of the bacterial chemosensory array obtained through the synthesis of cryo-electron tomography and large-scale molecular-dynamics simulations reveals a new kinase conformation during signaling events.
    1. Microbiology and Infectious Disease

    Cryo-electron tomography reveals novel features of a viral RNA replication compartment

    Kenneth J Ertel et al.
    Cryo-electron tomography unveils striking new structural components of positive-strand virus RNA replication compartments, greatly advancing mechanistic insights into the structure, assembly, function and control of these critical complexes.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cryo-EM visualization of the ribosome in termination complex with apo-RF3 and RF1

    Jesper Pallesen et al.
    Cryo-electron microscopy has been used to provide a structural interpretation of the complete action cycle of release factor 3 during translation termination, which includes a coordinated sequence of interactions with a class-I release factor and the ribosome.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM structures reveal specialization at the myosin VI-actin interface and a mechanism of force sensitivity

    Pinar S Gurel et al.
    Cryo-EM structures of actomyosin VI in multiple nucleotide states reveal a unique actin-myosin interface and a mechanism of force-sensitivity; furthermore, myosin VI remodels F-actin, suggesting a role for actin structural plasticity during force generation.
    1. Structural Biology and Molecular Biophysics

    Model-based local density sharpening of cryo-EM maps

    Arjen J Jakobi et al.
    Local sharpening of cryo-electron microscopy maps using atomic models can increase the interpretability of densities in cases of resolution variation and facilitates model building and refinement.

Refine your results by:

Type
Research categories