Colicin E1 opens its hinge to plug TolC
- University of Kansas, United States
- University of Michigan, United States
- Rutgers University, United States
- Rutgers, The State University of New Jersey, United States
Abstract
The double membrane architecture of Gram-negative bacteria forms a barrier that is impermeable to most extracellular threats. Bacteriocin proteins evolved to exploit the accessible, surface-exposed proteins embedded in the outer membrane to deliver cytotoxic cargo. Colicin E1 is a bacteriocin produced by, and lethal to, Escherichia coli that hijacks the outer membrane proteins TolC and BtuB to enter the cell. Here we capture the colicin E1 translocation domain inside its membrane receptor, TolC, by high-resolution cryoEM to obtain the first reported structure of a bacteriocin bound to TolC. Colicin E1 binds stably to TolC as an open hinge through the TolC pore-an architectural rearrangement from colicin E1's unbound conformation. This binding is stable in live E. coli cells as indicated by single-molecule fluorescence microscopy. Finally, colicin E1 fragments binding to TolC plug the channel, inhibiting its native efflux function as an antibiotic efflux pump and heightening susceptibility to three antibiotic classes. In addition to demonstrating that these protein fragments are useful starting points for developing novel antibiotic potentiators, this method could be expanded to other colicins to inhibit other outer membrane protein functions.
Data availability
The manuscript has been deposited in BioRXiv BIORXIV/2019/692251CryoEM maps and models have been deposited with accession codes EMD-21960, EMD-21959, PDB ID 6WXI, and PDB ID 6WXH. The following data are publically available for the two structures:6WXH TolC + colE1Structure: https://files.rcsb.org/download/6WXH.cifEM map: https://ftp.wwpdb.org/pub/emdb/structures/EMD-21959/map/emd_21959.map.gzValidation report: https://files.rcsb.org/pub/pdb/validation_reports/wx/6wxh/6wxh_full_validation.pdf6WXI TolC aloneStructure: https://files.rcsb.org/download/6WXI.cifEM map: https://ftp.wwpdb.org/pub/emdb/structures/EMD-21960/map/emd_21960.map.gzValidation report: https://files.rcsb.org/pub/pdb/validation_reports/wx/6wxi/6wxi_full_validation.pdf
Article and author information
Author details
Jacqueline J Stevens
Ayotunde P Ikujuni
Virangika K Wimalasena
Funding
National Institute of General Medical Sciences (DP2GM128201)
- Joanna SG Slusky
National Institute of General Medical Sciences (P20GM113117)
- Joanna SG Slusky
National Institute of General Medical Sciences (P20GM103638)
- Joanna SG Slusky
Gordon and Betty Moore Foundation (Moore Inventor Fellowship)
- Joanna SG Slusky
National Institute of General Medical Sciences (P20 GM103418)
- S Jimmy Budiardjo
National Institute of General Medical Sciences (2K12GM063651)
- S Jimmy Budiardjo
National Institute of General Medical Sciences (R21-GM128022)
- Julie S Biteen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- David Drew, Stockholm University, Sweden
Version history
- Preprint posted: July 4, 2019 (view preprint)
- Received: August 24, 2021
- Accepted: February 21, 2022
- Accepted Manuscript published: February 24, 2022 (version 1)
- Version of Record published: April 20, 2022 (version 2)
Copyright
© 2022, Budiardjo et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Colicin E1 opens its hinge to plug TolC
eLife 11:e73297.
https://doi.org/10.7554/eLife.73297
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