TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling

  1. Yan Tang
  2. Haihong Zong
  3. Hyokjoon Kwon
  4. Yunping Qiu
  5. Jacob B Pessin
  6. Licheng Wu
  7. Katherine A Buddo
  8. Ilya Boykov
  9. Cameron A Schmidt
  10. Chien-Te Lin
  11. P Darrell Neufer
  12. Gary J Schwartz
  13. Irwin J Kurland
  14. Jeffrey E Pessin  Is a corresponding author
  1. Departments of Medicine, Albert Einstein College of Medicine, United States
  2. East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, United States
  3. Departments of Neuroscience, Albert Einstein College of Medicine, United States
  4. The Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, United States
  5. Departments of Molecular Pharmacology, Albert Einstein College of Medicine, United States

Abstract

Cholinergic and sympathetic counter-regulatory networks control numerous physiological functions, including learning/memory/cognition, stress responsiveness, blood pressure, heart rate, and energy balance. As neurons primarily utilize glucose as their primary metabolic energy source, we generated mice with increased glycolysis in cholinergic neurons by specific deletion of the fructose-2,6-phosphatase protein TIGAR. Steady-state and stable isotope flux analyses demonstrated increased rates of glycolysis, acetyl-CoA production, acetylcholine levels, and density of neuromuscular synaptic junction clusters with enhanced acetylcholine release. The increase in cholinergic signaling reduced blood pressure and heart rate with a remarkable resistance to cold-induced hypothermia. These data directly demonstrate that increased cholinergic signaling through the modulation of glycolysis has several metabolic benefits particularly to increase energy expenditure and heat production upon cold exposure.

Editor's evaluation

The authors report results from timely studies, demonstrating the role of TIGAR in regulating thermoregulation. Deletion of TIGAR leads to resistance to cold-induced hypothermia. The results will be of wide interest.

https://doi.org/10.7554/eLife.73360.sa0

Introduction

TIGAR (Tp53-induced glycolysis and apoptosis regulator) was originally identified as a p53-inducible protein that functions as a fructose-2,6-bisphosphatase (F2,6P) but subsequently shown to have phosphatase activities for a variety of phosphorylated metabolic intermediates and allosteric regulators including 2,3-bisphospholgycerate (2,3BPG), 2-phosphoglycerate, phosphoglycolate, and phosphoenolpyruvate (Bensaad et al., 2006; Rigden, 2008; Bolaños, 2014; Tang et al., 2021). Due to its F2,6P dephosphorylation activity, TIGAR is generally considered a suppressor of glycolysis as F2,6P is a potent allosteric activator for 6-phosphofructo-1-kinase (PFK1), the rate-limiting step in glycolysis. However, the dephosphorylation of 2,3BPG to generate 3-phosphoglycerate would also be expected to increase glycolysis (Bolaños, 2014). In addition, the role of TIGAR in regulating carbohydrate metabolism is also complicated by the presence of the related phosphofructokinase bis-phosphatase (PFKBP) family that has both F6P 2-kinase and F2,6P bisphosphatase activities (Mor et al., 2011). Thus, the biological readout of TIGAR function is likely to be highly cell context dependent. In this regard, TIGAR has been reported to differentially modulate multiple different pathophysiological outcomes. For example, in the brain TIGAR expression was reported to protect against ischemic/reperfusion injury (Li et al., 2021), whereas in the heart TIGAR deficiency protected myocardial infarction (Hoshino et al., 2012) and in pressure-overloaded hearts (Okawa et al., 2019). In cancer models, TIGAR plays a complex role in the formation and progression of different types of cancer via suppressing aerobic glycolysis and controlling reactive oxygen species (ROS) production (Tang et al., 2021). Recently, it has been reported that TIGAR can both enhance the development of premalignances and suppress the metastasis of cancer invasion by the way of inhibition of ROS production (Cheung et al., 2020).

As carbohydrate metabolism and glycolysis are essential normal physiological processes in all cells and tissues, we have examined the metabolic phenotype of whole-body and tissue-specific TIGAR knockout mice. Surprisingly, we have observed that TIGAR deficiency in cholinergic neurons of mice results in a marked protection against hypothermia following an acute cold challenge. At a molecular level, this results from enhanced acetylcholine levels and increased cholinergic signaling at the neuromuscular junction (NMJ) driving skeletal muscle shivering-induced thermogenesis.

Results

Previously we reported that the TIGAR can modulate NF-kB signaling through a direct binding interaction and inhibition of the E3 ligase activity of the linear ubiquitin-binding assembly complex, LUBAC, in cultured cells (Tang et al., 2018). To examine the role of TIGAR in vivo, we initially generated whole-body Tigar knockout (TKO) mice. Surprisingly, however, during our phenotypic characterization we observed that the TKO male and female mice both display a remarkable resistance to cold induced-hypothermia. As shown in Figure 1, both male (panel A) and female (panel B) control mice when shifted from room temperature to a 4°C environment display the typical 4–5°C decline in core body temperature during the 1 hr acute exposure period. In contrast, the TKO male and female mice only display less than a 1°C decline in core body temperature under identical conditions. The level of Ucp1 mRNA (Figure 1—figure supplement 1A) and protein (Figure 1—figure supplement 1B) was not significantly different in interscapular brown adipose tissue (iBAT) at room temperature. Ucp1 mRNA did increase approximately 1.5-fold following 1 hr cold exposure, but this was not significantly different between the control and TKO mice. Although a very small amount of Ucp1 mRNA was detected in inguinal adipose tissue (iWAT) of control mice at room temperature, it was lower in the TKO mice (Figure 1—figure supplement 1C). Moreover, following 4°C exposure for 2 hr there was an increase in Ucp1 mRNA indicative of beige adipocyte induction. However, the induction of Ucp1 mRNA was significantly reduced in the TKO mice compared to the control mice, probably due to decreased sensitivity of cold-induced sympathetic activation (see Figure 7).

Figure 1 with 2 supplements see all
TIGAR deficiency mice are resistant to hypothermia induced by an acute cold challenge.

(A) Male mice (wild type [WT] n = 10, whole-body Tigar knockout [TKO] n = 6) and (B) female mice (WT n = 6, TKO n = 7) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4°C. (C) Representative TIGAR immunoblot of gastrocnemius muscle (Gastroc), epididymal white adipose tissue (EWAT), subcutaneous inguinal white adipose tissue (iWAT), and interscapular brown adipose tissue (iBAT) from the control Tigarfl/fl and adipocyte-specific knockout Tigarfl/fl/AdipoqCre mice. (D) Tigarfl/fl and Tigarfl/fl/AdipoqCre male mice (n = 6) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4°C. (E) Representative UCP1 and TIGAR immunoblots of brown adipose tissue from WT, TKO, Ucp1 knockout (UKO), and Ucp1 and Tigar double knockout (UTKO) mice from two independent genotypes each. (F) WT (n = 6), TKO (n = 6), UKO (n = 7), and UTKO (n = 8) mice core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4°C. (G) Representative TIGAR immunoblot of heart, gastrocnemius muscle (Gastroc), extensor digitorum digitorum longus (EDL), and soleus muscle from the control (Myl1Cre) and skeletal muscle-specific TKO (Myl1Cre/Tigarfl/fl) mice. (H) Myl1Cre and Myl1Cr>/Tigarfl/fl male mice (n = 6) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4°C. Statistical analyses are described in ‘Methods details,’ and the data are presented as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, §p<0.001.

Figure 1—source data 1

Fresh gastrocnemius muscle (Gastr), epididymal adipose tissue (EWAT), inguinal white adipose tissue, and interscapular brown adipose tissue from Tigarfl/fl and Tigarfl/fl>/AdipoqCre mice were collected, and 30 μg of the tissue lysate were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1C. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data1-v2.zip
Figure 1—source data 2

Interscapular brown adipose tissues were collected from wild type (WT), whole-body Tigar knockout (TKO), Ucp1 knockout (UKO), and Ucp1 and Tigar double knockout (UTKO) mice, and 30 μg of the tissue lysate were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1E. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data2-v2.zip
Figure 1—source data 3

Interscapular brown adipose tissues were collected from wild type (WT), whole-body Tigar knockout (TKO), Ucp1 knockout (UKO), and Ucp1 and Tigar double knockout (UTKO) mice, and 30 μg of the tissue lysate were used for UCP1 (33 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1E. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data3-v2.zip
Figure 1—source data 4

Interscapular brown adipose tissues were collected from wild type (WT), whole-body Tigar knockout (TKO), Ucp1 knockout (UKO), and Ucp1 and Tigar double knockout (UTKO) mice, and 30 μg of the tissue lysate were used for vinculin (117 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1E. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data4-v2.zip
Figure 1—source data 5

Fresh heart, gastrocnemius muscle (Gastr), extensor digitorum longus (EDL muscle), and soleus muscle were collected from Myl1Cre and Myl1Cre/Tigarfl/fl mice, and 30 μg of the tissue lysate were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1G. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data5-v2.zip
Figure 1—source data 6

Fresh heart, gastrocnemius muscle (Gastr), extensor digitorum longus (EDL muscle), and soleus muscle were collected from Myl1Cre and Myl1Cre/Tigarfl/fl mice, and 30 μg of the tissue lysate were used for vinculin (117 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 1G. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig1-data6-v2.zip

Changes in tissue distribution of muscle and fat mass as well as modulation of basal energy expenditure have important roles in the control of thermogenesis. However, there was no significant difference in the relative fat mass or lean mass (Figure 1—figure supplement 1D) between the control and TKO mice. Hematoxylin and eosin (H&E) staining of oxidative soleus and glycolytic extensor digitorum longus (EDL) (Figure 1—figure supplement 1E) skeletal muscle also indicated the absence of any gross morphological differences between the control and TKO mice. Similarly, food intake (Figure 1—figure supplement 1F), spontaneous locomotor activity in the AMB + Z dimensions (Figure 1—figure supplement 1G), and energy expenditure (Figure 1—figure supplement 1H) were essentially identical.

As the TKO mice are whole-body knockouts, the temperature phenotype could result from an effect on a single or combinatorial number of cell types. We first generated Tigarfl/fl mice that were crossed with AdipoqCre mice to generate adipocyte-specific TKO mice (Figure 1C). These mice had the identical temperature sensitivity as the control Tigarfl/fl mice both displaying an approximately 4°C decline in core body temperature following exposure to 4°C for 1 hr (Figure 1D). To further rule out BAT as a contributor to the enhanced cold protection in the TKO mice, we crossed the cold-sensitive Ucp1-deficient mice (Enerbäck et al., 1997) with the TKO mice (both strains with C57BL/6J background) to produce wild type (WT), TKO, UKO, and Ucp1-/-/Tigar-/- double knockout (UTKO) mice (Figure 1E). As expected, UKO mice were highly cold sensitive compared to their WT littermates following a 1 hr acute cold exposure (Figure 1F). Interestingly, the UTKO mice displayed a strong cold resistance, which was not significantly different from the TKO littermates. These data strongly suggest that thermogenic adipose tissue is not responsible for the resistance to hypothermia in the TKO mice.

Since skeletal muscle plays a major role in heat production, we next generated skeletal muscle-specific TKO mice by crossing the Tigarfl/fl mice with the skeletal muscle-specific myosin light polypeptide 1-Cre (Myl1Cre) mice (Figure 1G). TIGAR is highly expressed in skeletal muscle, with the highest protein levels in depots containing glycolytic fibers (white gastrocnemius and EDL) skeletal muscle. The Myl1Cre-driven TIGAR deletion resulted in efficient loss of TIGAR protein in all skeletal muscles examined with no effect on the heart (Figure 1G). Similar to the adipocyte-specific TKO mice, skeletal muscle Tigar-deficient mice had no significant resistance to acute cold exposure (Figure 1H). Moreover, we did not find any significant change in skeletal muscle mRNA expression of sarco-/endoplasmic reticulum Ca2+-ATPase (SERCA1, Atp2a1; SERCA2, Atp2a2) in control or TKO mice maintained at room temperature or shifted to 4°C for 1 hr (Figure 1—figure supplement 2A and B). Similarly, there was no change in myoregulin (Mrln) (Figure 1—figure supplement 2C). Although there was a small apparent increase in sarcolipin (Sln) mRNA in the control WT mice shifted to 4°C, there was no statistical difference with the TKO mice (Figure 1—figure supplement 2D). Together, these data are consistent with the absence of a cell-autonomous effect in either thermogenic adipocytes or skeletal muscle.

To unravel these unexpected findings, we next undertook a pharmacological approach to address the ability of the TKO mice to resist hypothermia. Treatment of control and TKO mice with the selective SERCA inhibitor cyclopiazonic acid (CPA) (Wang et al., 2021) reduced core body temperature at room temperature (Figure 2A). As expected, CPA treatment resulted in a faster rate of decline and to a lower extent when the mice were exposed to 4°C (Figure 2B). However, there was no significant difference in the response to CPA between the control and TKO mice. The CPA-induced reduction in body temperature is directly correlated with observable skeletal muscle paralysis, consistent with skeletal muscle contraction activity as an important component in thermal regulation (Blondin and Haman, 2018), and further indicates that SERCA functions downstream of TIGAR function.

Figure 2 with 2 supplements see all
TIGAR knockout mice are resistant to tubocurare but not cyclopiazonic acid (CPA).

(A) Wild type (WT) and whole-body Tigar knockout (TKO) male mice (n = 6) were intraperitoneally injected with CPA (10 mg/kg body weight), and then core body temperature was measured every 15 min at room temperature. (B) WT and TKO male mice (n = 6) were intraperitoneally injected with CPA (10 mg/kg body weight) at room temperature, and then shifted to 4°C for 30 min, with core body temperature measured every 15 min. (C) WT and TKO male mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight), and then core body temperature was measured every 10 minutes at room temperature. (D) WT and TKO male mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight) at room temperature, and then 10 min later shifted to 4°C for 30 min, with core body temperature measured every 10 min. (E) WT and TKO female mice (n = 6) were intraperitoneally injected with tubocurare (0.15 mg/kg body weight), and then core body temperature was measured every 15 min at room temperature. (F) WT and TKO male mice (n = 10) were intraperitoneally injected with different tubocurare doses, and the LD50 of curare was calculated using an online software LD50 Calculator (AAT Bioquest, Inc, Sunnyvale, CA). (G) WT and TKO male mice (n = 10) were intraperitoneally injected with a lethal tubocurare dose (0.6 mg/kg body weight), and the number of mice undergoing complete paralysis was plotted as a function of time in seconds. (H) The time to death (absence of respiration) of the same mice was plotted as a function of time in minutes. Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Since Tigar deficiency in neither adipocytes nor skeletal muscle recapitulated the protection of acute hypothermia but that paralysis results in a rapid reduction in core body temperature, we next assessed whether the cold resistance of the TKO mice was due to shivering thermogenesis (ST). To address this, we next treated mice with the highly specific skeletal muscle nicotinic acetylcholine receptor competitive antagonist tubocurare (Bowman, 2006). Control male mice treated with 0.4 mg/kg tubocurare became relatively inactive with essentially no detectable locomotor activity at room temperature 10 min post injection that lasted for another 20–30 min (see Figure 2—video 1). Over this time period, there was a concomitant decline in core body temperature that began to recover as the effects of tubocurare began to wear off (Figure 2C). Tubocurare treatment of the TKO male mice at room temperature also displayed a similar decline and recovery of body temperature but that remained somewhat higher than the control mice (Figure 2C). However, shifting the male mice to 4°C following tubocurare injection resulted in a rapid decline in body temperature such that by 30 min the control mice’s body temperature was reduced to less than 25°C (Figure 2D). In contrast, the tubocurare-injected TKO male mice only display a reduction in body temperature to approximately 34°C under identical conditions. Moreover, while the tubocurare-treated control mice placed at 4°C remained completely immobile, the TKO mice display apparent normal locomotor activity (Figure 2—video 1). Female mice are more sensitive to tubocurare than male mice (Maurya et al., 2013). The body temperature in WT female mice kept declining in 1 hr period after a lower dose (0.15 mg/kg) tubocurare injection at ambient temperature. Nevertheless, the decline of body temperature in the TKO female mice was resistant to the effects of tubocurare (Figure 2E).

The tubocurare dose-response for male mice is shown in Figure 2F, with the control WT mice having an LD50 of 0.44 mg/kg whereas the TKO mice having an LD50 of 0.55 mg/kg (Figure 2F). Injection of a lethal tubocurare dose (0.60 mg/kg) initially resulted in 50% if the control mice display a loss of voluntary locomotor activity by 150 s, but this required 240 s in the TKO mice (Figure 2G). At 10 min, 100% of the control mice were dead, whereas it took nearly 25 min for 100% of the TKO mice to die (Figure 2H). The tubocurare resistance of the TKO mice was not due to the changes in expression of the nicotinic acetylcholine receptor subunits with no significant differences in the skeletal muscle expression of the α1 (Chrna1), β1 (Chrnb1), or γ (Chrng) subunits (Figure 2—figure supplement 1A–C) and with a small reduction in the d (Chrnd) and ε (Chrne) subunits (Figure 2—figure supplement 1D and E) in the TKO mice. These data suggest that the protection against hypothermia was due to cholinergic signaling to the skeletal muscle.

Consistent with increased skeletal muscle energetic demand, ATP levels were significantly decreased with an apparent increase in ADP and AMP in the TKO mice after 1 hr at 4°C (Figure 3A). Stable isotope metabolic flux assessment with H2O18 demonstrated increased incorporation of orthophosphate into ATP, ADP, and AMP in the TKO mice at 4°C, which was not significantly different at room temperature (Figure 3B). Plasma creatine was also increased twofold in the TKO at 4°C (Figure 3C), reflecting the increased phosphocreatine turnover (CP M4 phosphate fragment m/z 83, Figure 3D). The appearance of creatine in the plasma is likely due, in part, to the increased rate of shivering that is known to induce skeletal muscle leakage (Baird et al., 2012).

Figure 3 with 2 supplements see all
TIGAR-deficient mice display increased ATP turnover in skeletal muscle.

(A) Wild type (WT) and whole-body Tigar knockout (TKO) male mice (n = 6) were maintained at room temperature (RT) or shifted to 4°C for 1 hr. Quadricep white skeletal muscles were isolated, and extracts assayed for ATP, ADP, and AMP levels as described in ‘Method details.’ (B) WT and TKO male mice (n = 3–4 per condition) were kept at RT or shifted to 4°C for 1 hr and then given an oral gavage of 0.3 ml pure H2O18 for 10 min at 4°C and an intraperitoneal (IP) injection of 1 ml pure H2O18 for another 10 min at 4°C. The quadricep muscles were freeze-clamped with liquid N2 and extracts prepared for mass spectroscopic analyses. The heatmap shows the O18 enrichment fraction of ATP, ADP, AMP, and creatine-phosphate. (C) WT and TKO male mice (n = 6) were maintained at RT or shifted to 4°C for 1 hr, and plasma creatine levels were determined by metabolomics analyses. (D) The CP_M4_83 data was generated from the same experimental setting as (B), indicating the increase in phosphocreatine turnover in TKO compared to that in WT at 4°C. Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. *p<0.05, ***p<0.01.

Figure 3—source data 1

Raw 18O enrichment data of stable isotope metabolic flux assessment with H218O was collected in quadricep white muscle of both ambient temperature housed and 4°C 1 hr exposed wild type (WT) and whole-body Tigar knockout (TKO) mice, as described in ‘Method details’.

The data was used in Figure 3.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig3-data1-v2.xlsx

The mechanics of energetic supply at 4°C was further examined by using a widely targeted LC/MS/MS protocol (Hoshino et al., 2012). Key pathways found to be significant were pentose phosphate pathway, purine nucleotide cycle, and amino acid utilization pathways (Figure 3—figure supplement 1A). The pentose phosphate pathway cycle fills the purine nucleotide cycle that acts in concert with the malate-aspartate shuttle and the TCA cycle to preserve cellular energetics (Hatazawa et al., 2015). In particular, glycine/serine, histidine, and methionine metabolism also drives the input of the tricarboxylic acid cycle intermediates serving as anapleurotic inputs (Figure 3—figure supplement 1B). Together, these interactions serve to recycle AMP generated by increased skeletal muscle energy demand.

Mitochondria are a primary source of both ATP production and heat generation during muscle contraction. To determine whether mitochondrial oxidative phosphorylation (OXPHOS) efficiency and/or capacity are altered in TKO mice, permeabilized skeletal muscle fiber bundles were prepared from red portions of the gastrocnemius muscle from the control and TKO mice immediately after 1 hr cold exposure. ADP-stimulated respiratory kinetics (i.e., Km and Vmax) were virtually identical between WT and TKO muscle during respiration supported by carbohydrate (Figure 3—figure supplement 2A and B) and/or lipid-based substrates. Rates of ATP production (JATP) and oxygen consumption (JO2) measured simultaneously under clamped submaximal and maximal ADP-demand states were also similar in muscle from the control and TKO mice (Figure 3—figure supplement 2C and D), yielding similar ATP/O efficiency ratios (Figure 3—figure supplement 2E). Finally, in vitro studies of the EDL muscle showed no differences in force-frequency, peak specific tension, or time to one-half relaxation between the control and TKO mice (Figure 3—figure supplement 2F and G). Together, these data indicate that the skeletal muscle contraction-induced thermogenesis in TKO mice is not due to any intrinsic change in skeletal muscle mitochondrial efficiency of contractile function and therefore must result from a change in signaling that in turn drives an enhanced skeletal muscle response.

Since the TKO mice are resistant to tubocurare and the major driver of skeletal muscle contraction is cholinergic signaling, we next generated cholinergic neuron-specific Tigar knockout (chTKO) mice using the choline acetyl-CoA transferase ChAT-Cre mice (Rossi et al., 2011). As the superior cervical ganglion (SCG) is composed of approximately 80% cholinergic neurons (Wang et al., 1990; Morales et al., 1995; Juranek and Wojtkiewicz, 2015), we collected the fresh SCGs (Figure 4—figure supplement 1) and confirmed that ChatCre resulted in the loss of Tigar in cholinergic neurons by immunofluorescence co-localization of TIGAR with the vesicular choline transporter (VChAT) (Figure 4A) and by immunoblotting analyses of TIGAR protein in the SCGs (Figure 4B). Similar to the TKO mice, the chTKO mice were also resistant to acute cold exposure compared to the control ChatCre mice (Figure 4C). Treatment with the SERCA inhibitor CPA, at room temperature (Figure 4D) or at 4°C (Figure 4E), also resulted in paralysis and a decline in core body temperature that was not significantly different between the ChatCre and chTKO mice, although the reduction in core body temperature was much greater at 4°C than at room temperature. The chTKO mice were also resistant to tubocurare at room temperature with both less of a core body temperature drop and a faster recovery (Figure 4F). In agreement with the TKO mice (Figure 2D), the resistance to cold-induced hypothermia by acute 4°C challenge was very dramatic in the chTKO mice compared to the control mice (Figure 4G). Similarly, the chTKO mice were highly resistant to the paralytic actions of tubocurare (Figure 4—video 1). Moreover, immunoblotting of the SCG for the neuron activation marker c-Fos further demonstrated that the control mice displayed a small increase in c-Fos protein levels when cold challenged that was further increased in the chTKO mice (Figure 4H). As controls, there were no significant differences in the protein levels of the vesicular acetylcholine transporter (VAChT) or the plasma membrane choline transporter (ChT) proteins. These data demonstrate that the cholinergic neuron TKO mice recapitulate the cold resistance and pharmacological characteristics of the whole-body TKO mice, consistent with these mice displaying increased cholinergic tone.

Figure 4 with 2 supplements see all
Cholinergic neuron-specific TIGAR knockout mice recapitulate the protection against hypothermia of the whole-body TIGAR-deficient mice.

(A) Representative immunofluorescent images showing TIGAR, vesicular acetylcholine transporter (VAChT), and nuclei (DAPI) in the superior cervical ganglions (SCGs) from the control ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) male mice. (B) Representative TIGAR, choline acetyltransferase (ChAT), and actin protein immunoblots of the SCG from two independent control ChatCre and chTKO male mice. (C) Male ChatCre and chTKO mice (n = 6) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4°C. (D) Male ChatCre and chTKO mice (n = 6) were intraperitoneally injected with cyclopiazonic acid (CPA,10 mg/kg body weight) at room temperature and core body temperature measured every 15 min. (E) ChatCre and chTKO male mice (n = 6) were intraperitoneally injected with CPA (10 mg/kg body weight) at room temperature, shifted to 4°C, and core body temperature measured every 15 min. (F) Male ChatCre and chTKO mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight), and then core body temperature was measured every 15 min at room temperature. (G) ChatCre and chTKO mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight) at room temperature and 10 min later shifted to 4°C for 30 min. Core body temperature was measured every 10 minutes. (H) Representative TIGAR, c-Fos, VAChT, ChT, and actin protein immunoblots of the SCG from two independent control ChatCre and chTKO male mice at room temperature or shifted to 4°C for 30 min. Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Figure 4—source data 1

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from the ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The left four lanes of the raw image (lysate from the mice at ambient temperature) were used for Figure 4B to confirm the efficiency of TIGAR protein loss in SCG of the chTKO mice. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data1-v2.zip
Figure 4—source data 2

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from the ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for choline acetyltransferase (ChAT, 70 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The left four lanes of the raw image (lysate from the mice at ambient temperature) were used for Figure 4B. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data2-v2.zip
Figure 4—source data 3

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from the ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for actin β (42 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The left four lanes of the raw image (lysate from the mice at ambient temperature) were used for Figure 4B. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data3-v2.zip
Figure 4—source data 4

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from both ambient temperature housed and 4°C 30 min exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The right eight lanes of the raw image (lysate from SCG tissues) was used for Figure 4H to confirm the efficiency of TIGAR protein loss in SCG of the chTKO mice. The left two lanes of the raw immunoblotting image represent the lysates of soluble fraction (RIPA lysis buffer extracted) of whole-brain tissues from the ChatCre and chTKO mice, respectively. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data4-v2.zip
Figure 4—source data 5

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from both ambient temperature housed and 4°C 30 min exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for c-Fos (62 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The right eight lanes of the raw image (lysate from SCG tissues) were used for Figure 4H to show the increase in c-Fos protein in SCG of the chTKO mice under cold exposed. The left two lanes of the raw immunoblotting image represent the lysates of soluble fraction (RIPA lysis buffer extracted) of whole-brain tissues from the ChatCre and chTKO mice, respectively. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data5-v2.zip
Figure 4—source data 6

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from both ambient temperature housed and 4°C 30 min exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for vesicular acetylcholine transporter (VAChT, 55 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The right eight lanes of the raw image (lysate from SCG tissues) were used for Figure 4H to show VAChT protein in the SCGs. The left two lanes of the raw immunoblotting image represent the lysates of soluble fraction (RIPA lysis buffer extracted) of whole-brain tissues from the ChatCre and chTKO mice, respectively. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data6-v2.zip
Figure 4—source data 7

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from both ambient temperature housed and 4°C 30 min exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for choline transporter (ChT, 55 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The right eight lanes of the raw image (lysate from SCG tissues) were used for Figure 4H to show ChT protein in that 63 and 70 kDa bands were observed in the SCGs. The left two lanes of the raw immunoblotting image represent the lysates of soluble fraction (RIPA lysis buffer extracted) of whole-brain tissues from the ChatCre and chTKO mice, respectively. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data7-v2.zip
Figure 4—source data 8

The superior cervical ganglion (SCG) tissues were collected and snap-frozen in liquid nitrogen from both ambient temperature housed and 4°C 30 min exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, as shown in Figure 4—figure supplement 1 for SCG dissection.

The SCGs from the three mice of the same strain were pooled and processed by beads homogenization to acquire tissue lysates. 15 μg of the tissue lysate were used for actin β (42 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section. The right eight lanes of the raw image (lysate from SCG tissues) were used for Figure 4H to show actin β protein in the SCGs. The left two lanes of the raw immunoblotting image represent the lysates of soluble fraction (RIPA lysis buffer extracted) of whole-brain tissues from the ChatCre and chTKO mice, respectively. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig4-data8-v2.zip

If increased cholinergic input is responsible for the increased energy demand and heat production by skeletal muscle, then the skeletal muscle metabolic characteristics of the TKO mice should be recapitulated in the chTKO mice. As shown in Figure 5, widely targeted LC/MS/MS in the gastrocnemius muscle of the chTKO mice identified the same three key pathways – pentose phosphate pathway, purine nucleotide cycle, and amino acid utilization pathways – that display a greater induction at 4°C. Together, these data are consistent with increased cholinergic signaling to skeletal muscle that is responsible for the increased skeletal muscle thermogenesis following cold exposure.

Skeletal muscles of the cholinergic neuron-specific Tigar knockout (chTKO) mice at 4°C display increased pentose phosphate pathway, purine nucleotide cycle, and amino acid utilization pathways.

The control ChatCre and chTKO male mice (n = 4–6) were maintained at room temperature (RT) or shifted to 4°C for 1 hr. Quadricep white skeletal muscles were isolated and extracts were subjected to widely targeted (multiple reaction monitoring [MRM]) small metabolite profiling using an ABSciex 6500 + QTRAP with ACE PFP and Merck ZIC-pHILIC columns as described in ‘Materials and methods.’ The heatmap shows the metabolites/pathways differentially identified with the corresponding p-values.

Figure 5—source data 1

Raw metabolites data were collected in the gastrocnemius muscle of both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice as described in ‘Method details’.

The data was used in Figure 5.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig5-data1-v2.xlsx

To address the basis of the apparent increase in cholinergic signaling, we first asked whether there was a change in synaptic density in the NMJ. The acetylcholine receptor aggregates in the synaptic cleft and can be specifically labeled with α-bungarotoxin (Pratt et al., 2018), as shown for the whole EDL muscle (Figure 6A). Quantification revealed a large degree of heterogeneity in the number of clustered acetylcholine receptors per muscle (Figure 6B). Nevertheless, there was a clear statistical increase in the TKO mice compared to the control mice. Consistent with the increase in synaptic junctions, the total amount of acetylcholine was also increased in skeletal muscle extracts from the TKO and chTKO mice compared to their respective control mice at both room temperature and after cold exposure (Figure 6C and D).

Figure 6 with 1 supplement see all
TIGAR deficiency increases acetylcholine biosynthesis and acetylcholine receptor clustering at the neuromuscular junction.

(A) Representative images of α-bungarotoxin immunofluorescent labeling of nicotinic acetylcholine receptor clusters in the extensor digitorium longus (EDL) muscle from wild type (WT) and whole-body Tigar knockout (TKO) mice. (B) Quantification of the number of nicotinic acetylcholine receptor clusters following 15 min exposure at 4°C (WT n = 17, TKO n = 13). These data represent the average of over six mice in each group of mean ± SD (unpaired t-test, two-tailed, **p=0.0035). (C) Acetylcholine levels in the gastrocnemius muscle of WT and TKO male (n = 7) mice at room temperature or following 1 hr at 4°C. (D) Acetylcholine levels in the gastrocnemius muscle of ChatCre and chTKO male (n = 6) mice at room temperature or following 1 hr at 4°C. (E) Representative immunoblots of TIGAR and actin proteins from two scrambled sgRNA and two Tigar sgRNA knockout Sh-SY5Y cell lines. (F) Acetylcholine concentrations in the medium of scrambled and TKO SH-SH5Y neuroblastoma cells. (G) m2 acetylcholine enrichments in cells labeled with d-glucose-1,2-13C2. (H) m2 acetylcholine enrichments in cells labeled with U-13C6 d-glucose. (I) m1 glutamate (m/z 128, C2-C4 fragment) enrichment in the medium of scrambled and TKO SH-SY5Y human neuroblastoma cells labeled with d-glucose-[1,2]-13 C2. Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. *p<0.05, ***p<0.001, ****p<0.0001.

Figure 6—source data 1

The culture SH-SY5Y cells were collected from both scrambled and whole-body Tigar knockout (TKO) cells, and 30 μg of the cell lysates were used for TIGAR (30 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The left four lanes of the raw image (lysate from neuroblastoma cells) were used for Figure 6E to confirm the efficiency of TIGAR protein loss in the SH-SY5Y neuroblastoma TKO cells. The right four lanes of the raw image represent the TIGAR immunoblotting of the cell lysates from 7-day differentiated neuroblastoma cells. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig6-data1-v2.zip
Figure 6—source data 2

The culture SH-SY5Y cells were collected from both scrambled and whole-body Tigar knockout (TKO) cells, and 30 μg of the cell lysates were used for actin β (42 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The left four lanes of the raw image (lysate from neuroblastoma cells) were used for Figure 6E to show actin β protein in the SH-SY5Y neuroblastoma cells. The right four lanes of the raw image represent the actin β immunoblotting of the cell lysates from 7-day differentiated neuroblastoma cells. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig6-data2-v2.zip

As it is not possible to determine the rate of acetylcholine synthesis in vivo, we took advantage of SH-SY5Y cells that have characteristics of cholinergic neurons (Kovalevich and Langford, 2013). Using sgRNA and Cas9, we effectively knocked out Tigar in several clonal SH-SY5Y cells, two representative control, and sgRNA knockout cells as shown in Figure 6E. The amount of acetylcholine secreted into the medium over a 24 hr period was increased in the TKO SH-SY5Y cells compared to controls (Figure 6F). Labeling with [1,2]-13C glucose demonstrates an increase in the m2-labeled acetylcholine levels in the TKO SH-SY5Y cells (Figure 6G). Similarly, labeling with U-13C glucose also demonstrated an increase in m2-labeled acetylcholine in the TKO SH-SY5Y cells (Figure 6H). Moreover, there was a threefold increase in the enrichment of the C2-C4 fragment, m1-glutamate m/z 129, indicating that the acetyl group in acetylcholine originated from glycolysis via pyruvate dehydrogenase (PDH) (Figure 6I, Figure 6—figure supplement 1A and B). This is because the m2 acetyl group formed via PDH generates 13C atoms at positions 4 and 5 of glutamate, whereas the m2 13C glutamate (m/z 130) at positions 2 and 3 is derived from pyruvate carboxylase (PC)-generated oxaloacetate (Madhu et al., 2020) schematically shown in Figure 6—figure supplement 1A. Analyses of the m/z 128–130 C2-C4 glutamate fragment distinguish these isotopomers with the enrichment of m1 glutamate (129 fragment) approximately five times more abundant than the m2 glutamate (130 fragment) in the TKO cells (Figure 6—figure supplement 1B).

If the changes in glucose flux occurred through increased flux through PDH, then we should also observe a concomitant increase in the levels of acetyl-CoA that is directly generated by PDH from pyruvate. As shown in Figure 6—figure supplement 1C, acetyl-CoA levels were increased approximately 1.5-fold and acetyl-carnitine levels approximately 2.5-fold (Figure 6—figure supplement 1D). Since acetyl-CoA is converted to acetyl-carnitine in the mitochondria, these demonstrate that Tigar deficiency not only increases glycolysis but also results in increased mitochondria uptake of acetyl-CoA (via PDH). The increase in mitochondria PDH flux would in turn drive an increase in oxygen consumption. Seahorse flux analyses of oxygen consumption rate (OCR) clearly demonstrated that the TKO SH-SY5Y cells had higher rates of OCR compared to the control SH-SY5Y cells (Figure 6—figure supplement 1E). Together, these data demonstrate that Tigar deficiency in SH-SY5Y neuroblastoma cells results from an increase in intracellular acetyl-CoA levels consistent with increased glycolysis and direct conversion of pyruvate to acetyl-CoA by PDH, as opposed to PC. It is the increase in PDH flux into the tricarboxylic acid cycle that drives increased mitochondria oxidation.

A generalized increased cholinergic tone would be expected to reduce blood pressure and heart rate but with increased skeletal muscle shivering. To assess these physiological parameters, we used implanted telemetry probes to simultaneously determine body temperature, mean arterial pressure (MAP), heart rate (beats per minute [BPM]), and skeletal muscle electromyography (EMG) in the cold-exposed ChatCre and chTKO mice (Figure 7). Consistent with the rectal temperature measurements previously observed, the telemetry probe also demonstrated that the chTKO mice are more resistant to cold-induced hypothermia than ChatCre mice (Figure 7A). At room temperature, there was no significant difference in blood pressure, but following cold exposure the blood pressure of the control mice increased, whereas the blood pressure of the chTKO mice decreased such that by 60 min the chTKO mice had a blood pressure of 25 mmHg less than the control mice (Figure 7B). Similarly, the heart rate was not statistically different at room temperature but increased substantially more in the control mice than in the chTKO mice following cold exposure (Figure 7C). As these data strongly suggest increased cholinergic signaling to enhance skeletal muscle contraction, we utilized EMG to directly determine skeletal muscle activity. A representative image of EMG tracers and root mean square (RMS) derivation is shown for the ChatCre and chTKO mice when shifted to 4°C for 30 min (Figure 7D). Quantification of these types of data as the integral of RMS during the time course of cold exposure demonstrated that at room temperature and following 10 min of cold exposure there is no significant difference in skeletal muscle contraction activity between the ChatCre and chTKO mice (Figure 7E). However, the contraction activity of the control ChatCre mice plateau at this level, whereas the chTKO mice shivering activity further increases to nearly twice the level of the control mice.

The cholinergic neuron-specific Tigar knockout (chTKO) mice display enhanced cold-stimulated skeletal muscle shivering activity and increased cholinergic tone.

(A) Male mice core body temperatures (ChatCre n = 7, TKO n = 10), (B) blood pressure (mmHg) (ChatCre n = 7, chTKO n = 10), (C) heart rate (beats per minute, BPM) (ChatCre n = 7, chTKO n = 6), (D) representative electromyography (EMG) traces and root mean squares (RMS) for ChatCre and chTKO male mice when shifted to 4°C for 30 min, and (E) neck EMG (ChatCre n = 5, chTKO n = 6) were measured continuously starting at ambient laboratory temperature (0 min) and for 60 min following placement at 4°C. (F) Extensor digitorium longus (EDL) muscles from two independent control ChatCre and chTKO male mice at room temperature (RT) or shifted to 4°C for 1 hr were isolated, and tissue extracts were immunoblotted with an antibody for the phospho-threonine 172 AMPKα subunit (pT172-AMPKα) or total α subunit (AMPKα). Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. **p<0.01.

Figure 7—source data 1

The extensor digitorum longus (EDL) muscles were collected from both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, and 30 μg of the tissue lysate were used for AMPKα (62 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 7F. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig7-data1-v2.zip
Figure 7—source data 2

The extensor digitorum longus (EDL) muscles were collected from both ambient temperature housed and 4°C 1 hr exposed ChatCre and cholinergic neuron-specific Tigar knockout (chTKO) mice, and 30 μg of the tissue lysate were used for phospho-Thr172AMPKα (62 kDa) immunoblotting analysis as described in the ‘Immunoblotting’ section.

The raw image was used for Figure 7F. A detailed description of the raw images is shown in Source data 1.

https://cdn.elifesciences.org/articles/73360/elife-73360-fig7-data2-v2.zip

The increased skeletal muscle shivering associated with increased ATP turnover and energy expenditure would be expected to result in a compensatory increase in AMP-dependent protein kinase (AMPK) activity. As shown in Figure 7F, there was no significant difference in the activation site phosphorylation (T172) of the AMPK α subunit in the EDL muscle at room temperature between the control ChatCre and chTKO mice. However, after 1 hr at 4°C, the control mice display the expected increase in T172-AMPKα phosphorylation that was further increased in the chTKO mice. Together, the relative changes in cold--induced blood pressure, heart rate, skeletal muscle shivering, and increase in AMPK activation are fully consistent with the chTKO mice displaying a generalized increase in cholinergic signaling following cold exposure.

Discussion

Currently, there is a substantial effort to understand and develop approaches to increase energy expenditure through the development/activation of brown/beige adipose tissue thermogenesis. A fraction of adult humans can respond to cold stress by inducing the expression of thermogenic adipose tissue under relatively long-term cold acclimation in adult humans (McNeill et al., 2021). However, it remains somewhat controversial if this inducible thermogenic human brown adipose tissue is the rodent equivalent of beige or brown adipocytes (Cannon et al., 2020; Samuelson and Vidal-Puig, 2020; Virtanen and Nuutila, 2021). In addition, whether the mass of the human-inducible thermogenic adipocytes is sufficient to significantly contribute to cold-responsive thermogenesis for core temperature maintenance or weight loss has also been questioned (Blondin et al., 2014; Virtanen and Nuutila, 2021). In contrast, skeletal muscle accounts for approximately 40% of healthy body mass and is the dominant factor for basal metabolic rate and the major driver of energy expenditure during physical exercise (Haman et al., 2010; Blondin et al., 2014). In rodents, non-shivering thermogenesis (NST) is driven by UCP1-dependent mitochondrial uncoupling in both brown and beige adipocytes by the creatine phosphorylation/dephosphorylation cycle in beige adipocytes (Cohen and Spiegelman, 2015; Kazak et al., 2015; Jung et al., 2019). There are also reports of a skeletal muscle-based NST via a sarcolipin-dependent futile cycle of SERCA-dependent calcium transport in the sarcoplasmic reticulum (Bal et al., 2012; Maurya et al., 2015). In humans, maximal NST can increase energy production equivalent to about two times above the resting metabolic rate (RMR), while moderate ST is 2.5–3.5 times RMR and maximal shivering has been measured at about five times RMR (Haman et al., 2010; Blondin et al., 2014). In contrast, exercise at ~50% VO2 max generates approximately 15 times RMR (Weber and Haman, 2005; Blondin et al., 2014). While a substantial effort has focused on NST, shivering and physical activity are the dominant forms of involuntary and voluntary heat production during acute cold exposure. Thus, it is essential to develop a deeper understanding of the physiological integrated role of skeletal muscle with thermogenic adipose tissue to have a complete and detailed molecular understanding of energy balance and its implications for potential interventions to promote and maintain weight loss.

The data presented in this article demonstrate that cholinergic neuron deficiency of Tigar results in an enhancement of cold-induced skeletal muscle contraction/shivering that is responsible for an increase in heat generation and protection of acute cold-induced hypothermia. This response is likely mediated by an increase acetylcholine signaling at the NMJ, consistent with the parallel induction of tubocurare resistance and increased cholinergic neuron activation. Interestingly, despite the increase in synaptic density and acetylcholine levels of mice at room temperature that are mildly cold stress (thermoneutrality for mice is approximately 29–31°C; Ganeshan and Chawla, 2017), Tigar deficiency does not appear to significantly affect body temperature, skeletal muscle contraction activity, or the overall skeletal muscle metabolite profile at room temperature. However, following acute cold challenge there are marked changes in these parameters between WT and cholinergic TKO mice including a metabolic profile indicative of increased ATP turnover. These findings suggest that there is an increase in cholinergic signaling reserve that only becomes apparent when cholinergic signaling demand is high.

In addition to the well-established role of sympathetic activation to increase brown and beige adipocytes thermogenesis (Zhu et al., 2019), both cholinergic and sympathetic activation can affect vascular blood flow and behavorial activities that also contribute to body temperature regulation (Nakamura et al., 2022). Although we cannot rule out the contribution of vasoconstriction to the conservation of core body temperature under cold exposure, heat loss from the mouse tail only accounts for 5–8% of whole-body heat loss (Skop et al., 2020) and is therefore unlikely to play a significant role in the thermoregulation observed by cholinergic Tigar deficiency.

In addition to TIGAR as a small carbohydrate phosphatase, previous studies have shown that TIGAR can directly interact with hexokinase 2 and the HOIP subunit of the linear ubiquitin assembly complex (Cheung et al., 2012; Tang et al., 2018). Thus, although it is formally possible that TIGAR increases cholinergic signaling due to alterations in protein-protein interactions, we believe that it is more likely a result of increased glycolysis due to its phosphatase function that is occurring in the cholinergic neurons as this would result in an increase in acetyl-CoA levels. The steady-state acetyl-CoA concentrations in neuronal mitochondrial and cytosol are several folds lower than the acetyl-CoA Km values for choline acetyltransferase. More specifically, following cholinergic differentiation there is a further decrease in acetyl-CoA levels that results in a further substrate dependence of acetylcholine biosynthesis (Ronowska et al., 2018). In contrast, several studies have also shown that choline uptake via the choline transporter is rate limiting for acetylcholine synthesis (Lockman and Allen, 2002). Although we have not measured choline uptake activity, Tigar deficiency had no significant effect on the amount of choline transporter protein. However, metabolic analyses clearly demonstrated an increased glucose-derived acetyl moiety into acetylcholine and increased steady-state acetylcholine levels with increased acetyl-CoA and acetyl-carnitine levels.

Thus, the simplest and most direct mechanism for the increase in acetylcholine levels induced by Tigar deficiency is the increased formation of acetyl-CoA through enhanced glycolysis, which in turn leads to increased production of acetylcholine in cholinergic neurons. At present, it is not possible to distinguish the amount of synaptic acetyl-CoA from skeletal muscle acetyl-CoA at the NMJ, but future studies using spatial mass spectroscopy (MALDI) may be able to resolve this issue.

Finally, it is also important to recognize that myasthenia gravis is a neuromuscular disease caused by autoantibodies against components of the NMJ and in particular the nicotinic acetylcholine receptor (Gilhus et al., 2019). Current therapeutic strategies are focused on the use of steroids or immune suppressants to block the immune system and drugs to increase the transmission of acetylcholine signaling such as acetylcholine esterase inhibitors (Gilhus et al., 2019). Similarly, central cholinergic neurons undergo severe neurodegeneration in Alzheimer’s disease and agents that enhance cholinergic signaling, particularly cholinesterase inhibitor therapies, providing significant symptomatic improvement in patients with Alzheimer’s disease (Ferreira-Vieira et al., 2016; Ahmed et al., 2019). Interestingly, recent reports show that the NMJs are stable in human age-related sarcopenia and in patients with cancer cachexia, which are different from the rodent models in that the sarcopenia is linked with the endplate fragmentation in NMJ (Jones et al., 2017; Boehm et al., 2020). As such, inhibition of TIGAR function may also provide a novel approach to improve skeletal muscle function, memory, and dementia through increased cholinergic signaling while limiting the deleterious metabolic consequences secondary to currently available therapies.

Materials and methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Escherichia coli)Mix & Go Competent Cells-Strain HB10Zymo ResearchT3013
Genetic reagent (Mus musculus)Tigar-/- (TKO)
(C57BL/6J)
PMCID:PMC5961042MMRRC, Cat# 063577-UCD
Genetic reagent (M. musculus)C57BL/6N-Tigartm1b(EUCOMM)Wtsi/WtsiWelcome Trust Sanger Institute (Hinxton Cambridge, UK)IMSR Cat# EM:09836;RRID:IMSR_ EM:09836
Genetic reagent (M. musculus)Myl1Cre:Myl1tm1(cre)Sjb/JThe Jackson LaboratoryRRID:IMSR_JAX:024713
Genetic reagent (M. musculus)ChatCre: B6J.129S6-Chattm2(cre)Lowl/MwarJThe Jackson LaboratoryRRID:IMSR_ JAX:028861
Genetic reagent (M. musculus)Tigarfl/fl(C57BL/6J)This studyN/ADescribed in ‘Mice models’
Genetic reagent (M. musculus)Tigarfl/flAdipoqCre (C57BL/6J)This studyN/ADescribed in ‘Mice models’
Genetic reagent (M. musculus)Tigarfl/flMyl1Cre (C57BL/6J)This studyN/ADescribed in ‘Mice models’
Genetic reagent (M. musculus)Tigarfl/fl/ChatCre (C57BL/6J)This studyN/ADescribed in ‘Mice models’
Genetic reagent (M. musculus)Ucp1-/- (UKO)(C57BL/6J)Laboratory of Victor L. SchusterAlbert Einstein
College of Medicine
Described in ‘Mice models’
Genetic reagent (M. musculus)Tigar-/-/Ucp1-/- (UTKO) (C57BL/6J)This studyN/ADescribed in ‘Mice models’
Cell line (Homo sapiens)SH-SY5Y neuroblastomaATCCATCC CRL-2266
Cell line (Homo sapiens)SH-SY5YControl neuroblastomaThis studyN/AMaterials and methods
Cell line (Homo sapiens)SH-SY5YTIGAR knockout neuroblastomaThis studyN/AMaterials and methods
AntibodyAnti-c-Fos (9F6) (rabbit monoclonal)Cell Signaling TechnologyCat# 2250S; RRID:AB_2247211(1:1000)
AntibodyRabbit anti-AMPKαCell Signaling TechnologyCat# 2532S; RRID:AB_330331(1:1000)
AntibodyRabbit anti-AMPKα phospho (Thr172)Cell Signaling TechnologyCat# 2535S; RRID:AB_331250(1:1000)
AntibodyAnti-UCP1 (EPR20381) (rabbit monoclonal)AbcamCat# ab209483; RRID:AB_2722676(1:2000)
AntibodyAnti-TIGAR (rabbit polyclonal)Millipore/SigmaCat# AB10545; RRID:AB_10807181(1:1000)
AntibodyAnti-VChAT (IF) (goat polyclonal)ImmunoStarCat# 24286; RRID:AB_572269(1:100)
AntibodyAnti-VChAT (rabbit polyclonal)Thermo Fisher ScientificCat# PA5-95404; RRID:AB_2807207(1:1000)
AntibodyAnti-ChAT (rabbit monoclonal)MilliporeSigmaCat# AB144P; RRID:AB_2079751(1:1000)
AntibodyRabbit anti-choline transporterThermo Fisher ScientificCat# PA5-77385; RRID:AB_2736619(1:1000)
AntibodyAnti-TIGAR (E-2) (mouse monoclonal)Santa Cruz BiotechnologyCat# sc-166290; RRID:AB_2066582(1:250)
AntibodyAnti-TIGAR (E-2) Alexa Fluor 488 (IF) (mouse monoclonal)Santa Cruz BiotechnologyCat# sc-166290 AF488(1:100)
AntibodyAlexa Fluor 594 donkey anti-goat IgG (H+L)Thermo Fisher ScientificCat# A-11058;
RRID:AB_2534105
(1:1000)
AntibodyAnti-β-actin (C4) (mouse monoclonal)Santa Cruz BiotechnologyCat# sc-47778; RRID:AB_626632(1:300)
AntibodyAnti-vinculin (7F9) (mouse monoclonal)Santa Cruz BiotechnologyCat# sc-73614;
RRID:AB 1131294
(1:300)
AntibodyGoat anti-mouse IgG-HRPSanta Cruz BiotechnologyCat# sc-2005(1:5000)
AntibodyGoat anti-rabbit IgG (H+L), HRPFisher ScientificCat# PI32460(1:5000)
AntibodyGoat anti-rabbit 800CWLI-CORCat#926-32211(1:6000)
AntibodyGoat anti-mouse 800CWLI-CORCat#926-32210(1:6000)
AntibodyDonkey anti-goat 800CWLI-CORCat#926-32214(1:6000)
Recombinant DNA reagent3xsgRNA/Cas9 all-in-one expression clone targeting TIGAR (NM_020375.2)GeneCopoeiaCat#HCP-215394-CG04-3
Recombinant DNA reagent3xsgRNA/Cas9 all-in-one expression clone scrambled sgRNA (control)GeneCopoeiaCat# CCPCTR01-CG04-B
Sequence-based reagentTigar<fl/fl> genotyping forwardThis studyIMSR Cat# HAR:7395;
RRID:IMSR_HAR:7395(9514-9534)
5’-AGGGGGTTG
CACCTCTATCTC
Sequence-based reagentTigar<fl/fl> genotyping reverseThis studyIMSR Cat# HAR:7395;
RRID:IMSR_HAR:7395(16933-16954)
5’-CACACAAG
AAGGAAGCTGTTGG
Commercial assay or kitDirect-zol RNA MiniPrep with 200 ml TRI ReagentZymo ResearchCat# R2053
Commercial assay or kitQuickDetect acetylcholine (ACh) (mouse) ELISA KitBioVision IncCat# E4453-100
Commercial assay or kitPowerPrep HP Plasmid Maxiprep Kits with PrefiltersOrigeneNP100025
Commercial assay or kitCeria Stabilized Zirconium Oxide BeadsMidSciCat# GB01
Commercial assay or kitProLong Gold Antifade Reagent with DAPICell Signaling TechnologyCat# 8961
Commercial assay or kitSuperScript IV VILO Master MixThermo Fisher Scientific InvitrogenCat# 11756050
Commercial assay or kitTaqMan Universal Master Mix II, no UNGThermo Fisher Scientific InvitrogenCat# 4440049
Chemical compound, drugTubocurarine hydrochloride pentahydrateMillipore/SigmaCat# T2379; CAS: 6989-98-6
Chemical compound, drugCyclopiazonic acid from Penicillium cyclopiumMillipore/SigmaCat# C1530; CAS: 18172-33-3
Chemical compound, drugd-Glucose-1,2-13C2Millipore/SigmaCat# 453188; CAS: 138079-87-5
Chemical compound, drugd-Glucose-13C6Millipore/SigmaCat# 389374; CAS: 110187-42-3
Chemical compound, drugMG-132Millipore/SigmaCat# 474787; CAS: 133407-82-6
Chemical compound, drugALLNMillipore/SigmaCat# 208719-25MG;
CAS:110044-82-1
Chemical compound, drugRIPA lysis bufferSanta Cruz BiotechnologyCat# sc-24948
Chemical compound, drugHalt Protease and Phosphatase Inhibitor CocktailThermo Fisher ScientificCat# 78442
Chemical compound, drugPierce Protein-Free T20 (TBS) Blocking BufferThermo Fisher ScientificCat# 37571
Chemical compound, drugSuperSignal West Pico Chemiluminescent SubstrateThermo Fisher ScientificCat# 34080
Chemical compound, drugSuperSignal West Femto Maximum Sensitivity SubstrateThermo Fisher ScientificCat# 34096
Chemical compound, drug4% paraformaldehyde solutionElectron Microscopy SciencesCat#RT157-4
Chemical compound, drugVectashield Antifade Mounting Medium with DAPIVector Laboratories, IncCat# H1200
Chemical compound, drugDMEM medium without glucose, glutamine, and sodium pyruvateBiological Industries USACat#01-0571-1A
Chemical compound, drugDMEM medium no glucose, glutamine, and sodium pyruvateBiological Industries USACat#01-0571-1A
Software, algorithmOdyssey Application Software 2.0LI-CORN/A
Software, algorithmGraphPad Prism 8GraphPad Softwarehttp://www.graphpad.com/scientific-software/prism/
Software, algorithmRQ Manager 1.2.1.Thermo Fisher Scientific Applied BiosystemsN/A
Software, algorithmLD50 CalculatorAAT Bioquesthttps://www.aatbio.com/tools/ld50-calculator
Software, algorithmVelocity softwareVelocity Software, Inchttps://www.velocitysoftware.com/
Software, algorithmMassHunterAgilent TechnologiesVersion: B08.00
Software, algorithmMultiQuantABSciexVersion: 3.0.2
Software, algorithmLabChart 8.1 for MacintoshADInstrumentshttps://www.adinstruments.com/products/labchart
OtherThermalert Model TH-8 Temperature MonitorPhysitemp Instruments LLC.Thermalert Model TH-8
OtherHD-X11 telemetric transponder probeDSI, Harvard Bioscience, Inchttps://www.datasci.com/products/implantable-telemetry/small-animal-telemetry
OtherDSI Implantable TelemetryDSI, Harvard Bioscience, Inchttps://www.datasci.com/products/implantable-telemetry/small-animal-telemetry
OtherGC-MS instrumentAgilent TechnologiesModel: 5975C
OtherDB-5MS columnAgilent TechnologiesCat#122-5532G
OtherLC-MS/MS instrumentABSciexModel: 6500+
OtherZIC-pHILIC columnMerckCat# 1.50460.0001

Resource availability

Lead contact

Request a detailed protocol

Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Jeffrey E Pessin (Jeffrey.pessin@einsteinmed.edu).

Materials availability

Request a detailed protocol

This study did not generate new unique reagents. SH-SY5Y neuroblastoma both scrambled and TKO cell lines are available upon request from the lead contact. Mouse lines generated in this study are available from the lead contact, although there are timeline restrictions to the availability of the mice due to the labor and space limitations of the laboratory staff and the facility of the Institution for Animal Studies at Albert Einstein College of Medicine.

Experimental models and subject details

Mice models

Request a detailed protocol

All studies were performed in accordance with protocols approved by the Einstein Institutional Animal Care and Use Committee. TKO mice were used as described in our previous paper (Tang et al., 2018). To establish conditional knockout mice of Tigar gene, we purchased C57BL/6N-TIGARtm1b(EUCOMM)Wtsi/Wtsi mice from the Welcome Trust Sanger Institute (Hinxton Cambridge, UK). Briefly, loxP sites were generated to delete exon 3 of Tigar, chimeric mice were mated with C57BL/6mice, and germline transmission was confirmed by PCR and Southern blotting. The progeny crossed sequentially with FLPe transgenic mice to remove lacZ and neo genes. The primer pairs TIGARCKO-5FRT-FW: TTGGGATCCCCTAGTTTGTG and TIGARCKO-5FRT-RV: AACTCAGCCTTGAGCCTCTG were used to confirm deletion of the lacZ and neomycin resistance genes. The primer pairs TIGARCKO-3loxp-FW: GAGAAGAGACCCCCTGGAAC and TIGARCKO-3loxp-RV: TTCCGGCCAAACAGACTTAC were used to confirm the Tigarfl/fl mice. Mice were backcrossed more than 10 generations to the C57BL/6J mice. The primers for subsequent genotyping for Tigarfl/fl are listed in the Key resources table, and the mouse tail PCR (touchdown cycling protocol, The Jackson Laboratory) results in 538 bp for mutant and 346 bp for the WT mice.

Adipocyte-specific TKO (Tigarfl/fl/AdipoqCre) mice were produced by mating Tigarfl/fl mice with Adiponectin-Cre (AdipoqCre) mice, as described previously (Feng et al., 2018). Skeletal muscle-specific TKO mice were produced by mating the Tigar<fl/fl> mice with skeletal muscle-specific myosin light polypeptide 1 Cre (Myl1Cre) mice purchased from The Jackson Laboratory (stock number 024713). chTKO mice were generated by mating Tigarfl/fl mice with ChatCre mice purchased from The Jackson Laboratory (stock number 028861). UKO mice, which were C57BL/6J background, were a gift from Dr. Victor Schuster (Albert Einstein College of Medicine). UTKO mice were generated by breeding UKO mice with TKO, which resulted in the production of WT, TKO, UKO, and UTKO mice.

Mouse husbandry and rectal temperature measurement

Request a detailed protocol

Mice were housed in a facility equipped with a 12 hr light/dark cycle. Animals were fed a normal chow diet that contains 62.3% (kcal) carbohydrates, 24.5% protein, and 13.1% fat (5053, LabDiet). The mice at 12–16 weeks of age were used for rectal temperature measurement using TH-8 Thermometer (Physitemp Instruments, Clifton, NJ) as per the manufacturer’s instructions. For cold exposure, the mouse was individually put into a pre-chilled cage with bedding in a cold chamber (4°C). For tissue collection, the mice were killed, and the tissues were collected and snap-frozen in liquid nitrogen and stored in a −80°C freezer. All studies were approved by and performed in compliance with the guidelines of the Albert Einstein College of Medicine Institutional Animal Care and Use Committee.

Blood plasma collection

Request a detailed protocol

Mice were briefly anesthetized by isoflurane, and the blood sample was collected from mouse orbital sinus into microtube with EDTA (Microvette 500 K3E, SARSTEDT, Germany) on ice. The plasma sample was collected by centrifugation (1000 × g, 30 min) and was stored at –80°C.

Gastrocnemius and quadricep skeletal muscle samples isolation

Request a detailed protocol

Gastrocnemius and quadricep white muscles were collected and snap-frozen in liquid nitrogen and stored at −80°C. The whole piece of the muscle was ground using a mortar-pestle in liquid nitrogen, and the muscle powder (30 mg) was used for metabolomics analysis.

Tubocurare and CPA treatments

Request a detailed protocol

Tubocurare (T2379, Sigma-Aldrich) was given as an intraperitoneal injection in a total volume of 100 μl in saline solution reaching at various doses and times indicated in the figure legends, and the median lethal dose (LD50) was calculated with LD50 Calculator, an online tool from AAT Bioquest (Sunnyvale, CA). CPA (C1530, Sigma-Aldrich) was given as an intraperitoneal injection in a total volume of 100 μl in saline solution reaching a dose of 10 mg/kg body weight for the time indicated. In both cases, the same volume of 100 μl of saline were injected as vehicle controls.

TIGAR knockout in human SH-SY5Y neuroblastoma cells

Request a detailed protocol

Human SH-SY5Y neuroblastoma cell line was purchased from ATCC (ATCC CRL-2266). The SH-SY5Y cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 1× penicillin-streptomycin. Cell lines were maintained in a 5% CO2 incubator at 37°C and were routinely tested to exclude Mycoplasma contamination.

The CRISPR 3xsgRNA/Cas9 all-in-one expression clone targeting Tigar (NM_020375.2) (Cat# HCP215394-CG04-3) and scrambled sgRNA control for pCRISPR-CG04 (Cat# CCPCTR01-CG04-B) were purchased from GeneCopoeia (Rockville, MD). The plasmid DNAs were transformed and amplified in Mix & Go Competent Cells-Strain HB 101 (Zymo Research, Cat# T3013, Irvine, CA) and purified using PowerPrep HP Plasmid Maxiprep kits with prefilters (Origene, Rockville, MD). The SH-SY5Y neuroblastoma cells were transfected with 10 μg of plasmid DNAs when 60% confluence in 100 mm dishes using transfection reagents GenJet II (SignaGen Laboratories, Rockville, MD) as per the manufacturer’s instructions. The transfected cells were cultured for 48 hr and collected in 500 μl of MACS buffer (phosphate-buffered saline [PBS-containing EDTA]) containing 1% fetal bovine serum in Falcon 5 ml polystyrene round-bottom tube filtered with a cell-strainer cap. The GFP-positive cells were selected using FACS GFP sorting and were cultured and proliferated in 10% FBS DMEM. The cell descent was collected, and cell lysate was immunoblotted with a TIGAR antibody.

Method details

In vivo 18O isotopic labeling of cellular phosphoryls of quardricep white muscle of the mice

Request a detailed protocol

WT and TKO male mice (n = 3–4 per condition) were kept at room temperature or shifted to 4°C for 1 hr and then given an oral gavage of 0.3 ml pure H218O for 10 min at 4°C and an IP injection of 1 ml saline H218O (0.9% NaCl in 18O water) for another 10 min at 4oC. The dosing and timing of this protocol were selected from pilot studies to ensure steady-state labeling of the ATP pool. The quadricep muscles were freeze-clamped with liquid N2 and extracts prepared for mass spectroscopic analyses for the identification of the position of 18O into the phosphates of ATP, which can be extrapolated to ADP and AMP (Nemutlu et al., 2015).

Purification and isotopic analysis of 18O-labeled cellular phosphoryls

Request a detailed protocol

Quadricep muscles were freeze-clamped and pulverized in mortar with liquid nitrogen, and extracted in 80% methanol solution. The samples were centrifuged at 10,500 rpm for 10 min at 4°C to precipitate proteins. The supernatants were transferred to an LCMS sampling vial pending for injection. Metabolite separation was performed on a ZIC-pHILIC column (Merck). Data was collected with ABSciex 6500 + QTRAP MS/MS with a multiple reaction monitoring (MRM) mode. The ions monitored are listed as follows:

MetabolitesIsotope labelsQ1Q3
AMPM034679
AMPM234881
AMPM425083
ADPM042679
ADPM242881
ADPM443083
ADPM0426159
ADPM2428161
ADPM4430163
ADPM6432165
ATPM050679
ATPM250881
ATPM451083
ATPM0506159
ATPM2508161
ATPM4510163
ATPM6512165
ATPM0506239
ATPM2508241
ATPM4510243
ATPM6512245
CPM021079
CPM221281
CPM421483

The 18O concentration in mouse body was determined with GC-MS according to the MMPC protocol.

Briefly, 10 µl of plasma from the matched quadricep muscle isolated mice were added to 2 µl of 5 N NaOH and 4 µl of % acetone in acetonitrile to allow for 16O to 18O exchange (Aksnes et al., 1966). The samples were kept at room temperature overnight (at least 10 hr). After chloroform extraction, the samples were analyzed on an Agilent GC-MS system with EI mode. Selected ion monitoring (SIM scan with ions of 58, 59, and 60) was used for the detection. A linear curve of different percentages (from 0% to 10%) of H218O in water was prepared along with the samples. 18O enrichment was calculated with the linear curve. The enrichment of 18O in phosphates in skeletal muscle (see below) was normalized to the 18O water enrichment in the plasma.

Stable isotope enrichment and acetylcholine analyses in culture media of SH-SY5Y control and TKO neuroblastoma cells

Request a detailed protocol

The SH-SY5Y neuroblastoma cells were cultured in 10 cm dishes until confluent and the culture media were removed, and the cells were washed with DMEM medium (Biological Industries, Boston, Cat# 01-057-1A, without glucose, glutamine, and pyruvate). Ten ml of DMEM containing either 10 mM d-glucose-1,2-13C2 (Sigma-Aldrich 453188-1G) or 10 mM d-glucose-13C6 (U-[13C]-glucose) and 10 mM d-glucose (unlabeled:labeled = 1:1), 1 mM glutamine, without pyruvate, was added to the cultured cells, and medium was collected at 4, 8, and 24 hr time points. The medium was centrifuged at 1000 × g for 20 min at 4°C and stored at –80°C. The plates at 24 hr time point were put on ice and gently rinsed with 1.5 ml of ice-cold 150 mM ammonium acetate. The cells were scraped and transferred into a 2 ml screw cap tube (Cat# 02682558, Thermo Fisher). The cell pellets were collected by 1000 × g centrifugation at 4oC for 2 min and stored at –80°C for cellular acetyl-CoA and acetyl carnitine measurement.

Following thawing on ice, the medium was subjected to an ethyl chloroformate (ECF) derivatization according to our developed procedure with a minor modification (Xie et al., 2007). Briefly, a volume of 700 µl of cell culture media was added to 500 µl of ethanol:pyridine = 4:1, and 100 µl of ECF. After a brief vortex, the samples were sonicated for 1 min. The derivatized metabolites were extracted with 300 µl of chloroform and injected 1 µl into the GC-MS system in splitless mode (GC-MS, Agilent, USA). Helium was used as a carrier gas at a consistent flow of 1 ml/min. The injection temperature was set at 260°C, and metabolite separation was performed on an Agilent DB-17MS column. The oven program started at 60°C for 0.5 min and rose to 100°C at a rate of 20°C/min, and then to 300°C at a rate of 10°C/min, and then maintained at 300°C for 4.5 min. The data was analyzed with MassHunter Quantitative Analysis software (Agilent).

The m0 glutamate fragment m/z of 128 contains three carbons (carbon 2–4) out of the five carbons, while fragment m/z of 202 contains four carbons (carbon 2–5) from the whole glutamate molecule. M1 of glutamate at m/z 129 reflects the presence of 13C at the fourth carbon position. This reflects acetyl units originating from glucose-> pyruvate entering through PDH for eventual oxidation (glycolytic flux for energy generation). M2 of glutamate m/z 130 reflects the presence of 13C at the second and third carbon positions, and reflects pyruvate entering the oxaloacetate pool or through PC (Madhu et al., 2020; Silagi et al., 2020). M2 of glutamate at 204 is the overall TCA cycle flux. Those two 13C come from either pyruvate entering from PC or PDH. For lactate, the m/z of M0 to M2 for carbon 2 and 3 positions will be 145–147 (Madhu et al., 2020). A pooled quality control (QC) sample was injected six times for coefficient of variation (CV) determination. Metabolites with CVs lower than 30% were included for quantification while loss with CVs greater than 30% were considered indeterminant. These data were obtained using a Sciex 6500 + QTRAP with ACE PFP and Merck ZIC-pHILIC columns as described previously (Hoshino et al., 2012).

Acetylcholine enrichment in culture media of SH-SY5Y control and TKO neuroblastoma cells

Request a detailed protocol

The cell culture medium samples were thawed on ice and 200 µl was added to 800 µl of acetonitrile plus 10 µl of 10 ng/ml acetylecholine_d9 (deuterium 9 as an internal control). The samples were vortex, centrifuged at 14,000 rpm for 10 min, and supernatants were transferred into glass vials. The samples were analyzed with ABSciex 6500 + with ACE PFP column. The MRMs for m0, m1, m2 for acetylcholine and the internal standard are as given in the following table. Data was processed with multiquanta software (ABSciex). The enrichment was calculated after subtracting from the background of the non-labeled treatment samples.

MRM table

Request a detailed protocol
Q1 mass (Da)Q3 mass (Da)
1Acetylcholine146.387.0
2Acetylcholine_m1147.388.0
3Acetylcholine_m2148.389.0
4(d9) Acetylcholine155.387.0

Cellular acetyl-CoA and acetyl-carnitine measeurement in SH-SY5Y control and TKO neuroblastoma cells

Request a detailed protocol

The cell pellet samples were added with 80% methanol with internal standards (malonyl CoA_13C3, and acetyl-carnitine_d3) extraction with three cycles of frozen (liquid nitrogen)-thaw (sonication). The samples were vortex, centrifuged at 21,000 × g for 10 min, and the supernatants were transferred into glass vials. The samples were analyzed with ABSciex 6500 + iHILIC-p column (HILICON). Data was processed with multiquanta software (ABSciex).

Total RNA extraction and quantitative RT-PCR

View detailed protocol

Cellular and tissue total RNA was extracted using TRI reagent and Direct-zol RNA MiniPrep Plus kit (Zymo Research). First-strand cDNA was synthesized using the SuperScript IV VILO cDNA synthesis kit (Thermo Fisher Scientific). TaqMan RT-PCR was performed for the measurement of mRNA using the ΔΔCt method. Gene expression was adjusted by comparison with Rpl7 expression. The quantitative RT-PCR results were analyzed with RG Manager version 1.2.1 (Applied Biosystems). Primer-probe mixtures for the genes Ucp1, Atp2a1 (SERCA1), Atp2a2 (SERCA2), Sln (sarcolipin), Mrln (myoregulin), Chrna1, Chrnb1, Chrnd, Chrne, and Chrng are listed as follows:

Gene nameGene nameTaqMan assay ID
Uncoupling protein 1 (mitochondrial, proton carrier)Ucp1Mm01244861_m1
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 (SERCA1)Atp2a1Mm01275320_m1
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (SERCA2)Atp2a2Mm01201431_m1
SarcolipinSlnMm00481536_m1
MyoregulinMrlnMm01175781_m1
Cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)Chrna1Mm00431629_m1
Cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)Chrnb1Mm00680412_m1
Cholinergic receptor, nicotinic, delta polypeptideChrndMm00445545_m1
Cholinergic receptor, nicotinic, epsilon polypeptideChrneMm00437411_m1
Cholinergic receptor, nicotinic, gamma polypeptideChrngMm00437419_m1

Primer-probe mixture for Rpl7 was customized, and other primer-probe mixtures were obtained from Thermo Fisher Applied Biosystems.

Immunoblotting

Request a detailed protocol

Commercial primary antibodies were purchased for the detection of TIGAR (Cat# AB10545, Millipore/Sigma; Cat# sc-677290, Santa Cruz), ChAT (Cat# AB144P, Millipore/Sigma), VAChT (Cat# 24286, ImmunoStar), ChT (Cat# PA5-77385, Thermo Fisher), c-fos (2250S, Cell Signaling; MA5-15055, Invitrogen), phosphor-c-FosSer32 (5348S, Cell Signaling), AMPKα (2532S, Cell Signaling), phospho-threonine 172 AMPKα (2535S, Cell Signaling), UCP1 (ab209483, Abcam), vinculin (sc-73614), and β-actin (sc-47778, Santa Cruz). Samples were prepared from culture cells (washed by cold PBS) or tissues by homogenization with a radioimmune precipitation assay lysis buffer (sc-24948, Santa Cruz Biotechnology) containing Halt protease and phosphatase inhibitor mixture (Cat# 78442, Thermo Fisher Scientific), 100 μm MG132, and 100 μm ALLN (EMD Millipore, Darmstadt, Germany) using Ceria stabilized zirconium oxide beads (MidSci, Valley Park, MO). Homogenates were centrifuged for 15 min at 21,000 × g at 4°C, and supernatants were collected for the protein assay using the BCA method. Protein samples were separated by SurePAGE (Bis-Tris) precasted gel (GenScript, Piscataway, NJ) and transferred to nitrocellulose membrane using iBlot Blotting System (Thermo Fisher Scientific). The immunoblot membrane was blocked with Pierce Protein-Free T20 (TBS) blocking buffer (product no. 37571, Thermo Fisher Scientific) and incubated with the first antibody indicated in the blocking buffer. Blots were washed in TBS with Tween 20 (TBST) and incubated with either IRDye 800CW secondary antibody (LI-COR, Lincoln, NE) or horseradish peroxidase-conjugated secondary antibody in blocking buffer. The membrane was washed with TBST and visualized either by the Odyssey Imaging System (LI-COR) or enhanced chemiluminescence (ECL) (Thermo Fisher Scientific Pierce) method. ImageJ was used to quantify protein bands on the membrane.

Fluorescence imaging of nicotinic acetylcholine receptors clustering at neuromuscular junctions

Request a detailed protocol

Whole EDL muscles were fixed 30 min with 4% w/v paraformaldehyde after dissection. After PBS washing 5 min × three times, EDL muscles were incubated with 1:1000 dilution of Alexa Fluor 488 conjugate α-Bungarotoxin (Invitrogen B13422) for 2 hr at room temperature, and mounted onto 35 mm glass-bottom dish (MatTek Corporation, Cat# P35G020C.S) with Vectashield Antifade Mounting Medium with DAPI (Cat# H1200, Vector Laboratories, Inc, Burlingame, CA). The NMJ was visualized using a z-series projection on a confocal microscope (Leica SP8) with ×40 objective. Velocity software was used for image analysis.

Immunofluorescence imaging of SCG

Request a detailed protocol

The ChatCre and chTKO male mice (14 weeks old) were euthanized and the fresh SCG tissues were removed (Figure 4—figure supplement 1) and embedded in optimal cutting temperature compound. The frozen tissue cross-sections (10 μm) were blocked with 3% bovine serum albumin in PBS for 60 min at room temperature. For VChAT and TIGAR immunofluorescence staining, the SCG section was incubated with the mouse monoclonal anti-TIGAR (E-2) Alexa Fluor 488 (sc-166290 AF488, Santa Cruz Biotechnology) and goat polyclonal anti-VChAT (Cat# 24286, ImmunoStar, Hudson, WI) for 2 hr at room temperature. After washing PBS 5 min × three times, Alexa Fluor 594 donkey anti-goat IgG (H+L) (1:1000, Cat# A-11058, Thermo Fisher Scientific) was added to the sections for 30 min at room temperature. The slides were mounted on coverslips with Vectashield Antifade Mounting Medium with DAPI (Cat# H1200, Vector Laboratories, Inc) and visualized using a z-series projection on a confocal microscope (Leica SP8) with ×40 objective.

Synchronized measurement of core body temperature, blood pressure, heart beating rate, and electromyography

Request a detailed protocol

ChatCre and chTKO mice were anesthetized with isoflurane and implanted with DSI HD-X11 telemetric transponder probes according to the manufacturer’s guidelines, with the blood pressure probe placed in the descending aorta, the EMG leads run subcutaneously to the neck muscles bilaterally, and the core temperature probe placed intraperitoneally. Animals were returned to a standard temperature housing room maintained at 21–22°C on a 12 hr/12 hr light:dark cycle, lights on at 0700. Mice were individually housed on cob bedding in standard polycarbonate home cages that were placed on telemetric DSI platforms to continuously monitor arterial blood pressure, heart rate, core body temperature, and neck EMG muscle activity from the implanted DSI XD-11 probes using LabChart 8.1 software for Macintosh. After 1 week recovery from surgery, animals’ home cage bedding was removed and replaced by stainless steel mesh flooring 1 inch above the base of the cage. Food but not water was then removed from the home cage, and animals in their home cages on DSI platforms were then placed in a 10' × 10' environmental cold room maintained at 4°C, 35% humidity for 1 hr from 10:00 to 11:00 am, and continuous measurements of heart rate, blood pressure, core temperature, and EMG activity continued. At the end of the 1 hr cold challenge, mice in their individual cages were then returned to the standard temperature housing room, and food and bedding were replaced.

Mitochondrial OXPHOS efficiency and capacity

View detailed protocol

Respiration of permeabilized muscle fibers was performed as described previously (Heden et al., 2017; Johnson et al., 2018; Heden et al., 2019). Briefly, a small portion of freshly dissected red gastrocnemius muscle tissue was placed in 7.23 mM K2EGTA, 2.77 mM Ca K2EGTA, 20 mM imidazole, 20 mM taurine, 5.7 mM ATP, 14.3 mM phosphocreatine, 6.56 mM MgCl2.6H2O, and 50 mM K-MES (pH 7.1). Fiber bundles were separated and permeabilized for 30 min at 4°C with saponin (30 μg/ml) and immediately washed in 105 mM K-MES, 30 mM KCl, 10 mM K2HPO4, 5 mM MgCl2.6H2O, BSA (0.5 mg/ml), and 1 mM EGTA (pH 7.4) for 15 min. After washing, high-resolution respiration rates were measured using an OROBOROS Oxygraph-2k.

Muscle contraction/relaxation

Request a detailed protocol

The EDL muscle was dissected and tied into the Horizontal Tissue Bath System from Aurora Scientific Inc as described previously (Ferrara et al., 2018). The muscles were stimulated with a 20 V twitch train and stretched until optimal length for force production was reached. Muscles were then allowed to equilibrate for 5 min. For force-frequency curves, muscles were stimulated with frequencies ranging from 10 to 200 Hz (0.1 ms pulse, 330 ms train, and 2 min between trains). Forces produced by electrically stimulated muscle contractions were recorded in real time via a force transducer (model 400 A, Aurora Scientific Inc). The specific force was calculated using the cross-sectional area of the muscle tissue (millinewton per square millimeter), as estimated from the weight and length of the muscle.

Gastrocnemius muscle acetylcholine assay

Request a detailed protocol

Fresh gastrocnemius muscle was collected and snap-frozen in liquid nitrogen, and the whole piece of the muscle was ground to powder using a mortar-pestle in liquid nitrogen. The muscle powder was then homogenized in PBS (pH 7.4) using Ceria stabilized zirconium oxide beads (MidSci) and the homogenate was centrifuged for 20 min at 3000 rpm at 4°C. The supernatant was collected, and the protein assay was performed using the Pierce BCA Protein Assay kit (Cat# 23225, Thermo Fisher Scientific). The supernatants from different samples were adjusted to the same protein concentration of protein, and the same volume of the supernatants was subjected to acetylcholine assay using QuickDetect acetylcholine (ACh) (mouse) ELISA Kit (Cat# E4453-100, Biovision, Milpitas, CA).

Body composition and calorimetry

Request a detailed protocol

Male WT C57BL/6J and TKO mice (n = 4) were internally transferred to the Animal Energy Balance Phenotyping Core of the New York Obesity Research Center at the Albert Einstein College of Medicine. In vivo body composition was determined by magnetic resonance spectroscopy using an ECHOMRI instrument (Echo Medical Systems). The mice were then individually housed in metabolic chambers maintained at 21–22°C on a 12 hr light/12 dark cycle with lights on at 7 am and were provided with a nutritionally complete standard powdered diet (NIH 07) and tap water ad libitum for five consecutive days. Metabolic and behavioral measurements (oxygen consumption, food intake, locomotor activity) were obtained continuously using a CLAMS (Columbus Instruments) open-circuit indirect calorimetry system (Li et al., 2011).

Measurement of OCR in SH-SY5Y control and TKO neuroblastoma cells

Request a detailed protocol

Real-time OCR was simultaneously assessed in SH-SY5Y control and TKO neuroblastoma cells using a Seahorse Bioscience XF96 extracellular flux analyzer (Zhang and Zhang, 2019). Three batches of control CTL (10 wells/batch) and TKO (10 wells/batch) cells, respectively, were performed for the OCR assay. The cell suspension of 5 × 104 in 50 μl of DMEM with 10% fetal bovine serum was seeded into a well of the XF 96-well microplates to reach confluence growing an even monolayer. 16 hr later, 200 μl/well of medium were added. The cells were at 95–100% confluence and subjected to Seahorse analysis at the Stable Isotope & Metabolomics Core Facility at the Albert Einstein College of Medicine. Briefly, cells were rinsed and incubated with XF base medium (Agilent, 102353-100) supplemented with 25 mM glucose, 2 mM l-glutamine (Thermo Fisher Scientific, 25030081) and 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), pH 7.4. After 45 min incubation in a CO2-free incubator at 37°C, OCR was recorded at baseline, followed by sequential additions of 1 μM oligomycin (Sigma-Aldrich, 75351), 1 μM FCCP (Sigma-Aldrich, C2920), and 1 μM rotenone (Sigma-Aldrich, R8875). The OCR was automatically recorded and calculated using the Seahorse XF24 software, and the data were exported into Prism 8.1 software for graph production and statistical analysis.

Soleus and EDL muscles crossed section HE staining

Request a detailed protocol

Fresh soleus and EDL skeletal muscles from 4-month-old male WT and TKO mice were isolated and fixed in 10% formalin for 48 hr at 4°C. The muscle samples were then switched to 70% alcohol and subjected to H&E staining at the Histology and Comparative Pathology Core at the Albert Einstein College of Medicine. Briefly, fixed muscle samples were embedded in cassettes perpendicularly with paraffin. The block was sectioned 10 µm thick, and the sections were mounted on slides, H&E stained, and covered with clips. The H&E-stained images were captured with a digital microscope camera.

Quantification and statistical analyses

Request a detailed protocol

Prism (8.1) GraphPad Software was used for data processing, analyses, and graph productions in the experiments. To analyze the differences of body temperature at different time points among two to four groups of the mice, the tabs of Grouped Analyses/two-way ANOVA (or mixed model)/multiple comparison were selected. The subtab ‘Compare each cell mean with the other cell mean in that row’ was chosen to show the ‘Sidak’s multiple comparison test,’ which summarized the statistical test results. The number of independent experimental replications and the average with standard deviation are provided in the figure legends. Unpaired two-tailed p-value t-tests were used for the statistical tests between the two groups. The statistical analyses were made at significance levels as follows: ns, not statistically significant; *p<0.5; **p<0.1; ***p<0.001, and ****p<0.0001.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting file; Source Data files have been provided for Figures 1, 3, 4, 5, 7, Figure 1-figure supplement 1, 3, and 6.

References

    1. Bowman WC
    (2006) Neuromuscular block
    British Journal of Pharmacology 147 Suppl 1:S277–S286.
    https://doi.org/10.1038/sj.bjp.0706404

Article and author information

Author details

  1. Yan Tang

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1377-422X
  2. Haihong Zong

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – original draft
    Competing interests
    No competing interests declared
  3. Hyokjoon Kwon

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft
    Competing interests
    No competing interests declared
  4. Yunping Qiu

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – original draft
    Competing interests
    No competing interests declared
  5. Jacob B Pessin

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Formal analysis, Investigation, Methodology, Software, Validation, Writing – original draft
    Competing interests
    No competing interests declared
  6. Licheng Wu

    Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization
    Competing interests
    No competing interests declared
  7. Katherine A Buddo

    East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, Greenville, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation
    Competing interests
    No competing interests declared
  8. Ilya Boykov

    East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, Greenville, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation
    Competing interests
    No competing interests declared
  9. Cameron A Schmidt

    East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, Greenville, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation
    Competing interests
    No competing interests declared
  10. Chien-Te Lin

    East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, Greenville, United States
    Contribution
    Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation
    Competing interests
    No competing interests declared
  11. P Darrell Neufer

    East Carolina Diabetes and Obesity Institute and the Department of Physiology, Brody School of Medicine East Carolina University, Greenville, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – original draft
    Competing interests
    No competing interests declared
  12. Gary J Schwartz

    1. Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    2. Departments of Neuroscience, Albert Einstein College of Medicine, Bronx, United States
    3. The Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Writing – original draft
    Competing interests
    No competing interests declared
  13. Irwin J Kurland

    1. Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    2. The Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Writing – original draft
    Competing interests
    No competing interests declared
  14. Jeffrey E Pessin

    1. Departments of Medicine, Albert Einstein College of Medicine, Bronx, United States
    2. The Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, Bronx, United States
    3. Departments of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review and editing
    For correspondence
    jeffrey.pessin@einsteinmed.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2041-2726

Funding

National Institutes of Health (DK033823)

  • Jeffrey Pessin

National Institutes of Health (DK020541)

  • Jeffrey Pessin

S10 SIG Award for the Sciex 6500+QTRAP (1S10OD021798)

  • Irwin J Kurland

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Dr. Yu Zhang at the Flow cytometry Core Facility (Albert Einstein College of Medicine) for SH-SY5Y cells FACS sorting. We thank Dr. Xue-liang Du at the Stable Isotope & Metabolomics Core Facility for Seahorse analyses. We also thank Dr. Victor Schuster (Albert Einstein College of Medicine) for the gift of UCP1 knockout mice. We thank Ms. Nicole Fernandez (Ph.D. candidate at the Albert Einstein College of Medicine) for her contribution to the production of Graphic Abstract. This study was supported by grants DK033823 and DK020541 from the National Institutes of Health, and an S10 SIG Award for the Sciex 6500 + QTRAP (1S10OD021798).

Ethics

All studies were performed in accordance with protocols approved by the Einstein Institutional Animal Care and Use Committee. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (0000-1041, 0000-1061, and 0000-1389) of the Albert Einstein College of Medicine.

Copyright

© 2022, Tang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,060
    views
  • 198
    downloads
  • 2
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Yan Tang
  2. Haihong Zong
  3. Hyokjoon Kwon
  4. Yunping Qiu
  5. Jacob B Pessin
  6. Licheng Wu
  7. Katherine A Buddo
  8. Ilya Boykov
  9. Cameron A Schmidt
  10. Chien-Te Lin
  11. P Darrell Neufer
  12. Gary J Schwartz
  13. Irwin J Kurland
  14. Jeffrey E Pessin
(2022)
TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife 11:e73360.
https://doi.org/10.7554/eLife.73360

Share this article

https://doi.org/10.7554/eLife.73360

Further reading

    1. Cell Biology
    2. Stem Cells and Regenerative Medicine
    Nathaniel Paul Meyer, Tania Singh ... Diane L Barber
    Research Article

    Our understanding of the transitions of human embryonic stem cells between distinct stages of pluripotency relies predominantly on regulation by transcriptional and epigenetic programs with limited insight on the role of established morphological changes. We report remodeling of the actin cytoskeleton of human embryonic stem cells (hESCs) as they transition from primed to naïve pluripotency which includes assembly of a ring of contractile actin filaments encapsulating colonies of naïve hESCs. Activity of the Arp2/3 complex is required for the actin ring, to establish uniform cell mechanics within naïve colonies, promote nuclear translocation of the Hippo pathway effectors YAP and TAZ, and effective transition to naïve pluripotency. RNA-sequencing analysis confirms that Arp2/3 complex activity regulates Hippo signaling in hESCs, and impaired naïve pluripotency with inhibited Arp2/3 complex activity is rescued by expressing a constitutively active, nuclear-localized YAP-S127A. Moreover, expression of YAP-S127A partially restores the actin filament fence with Arp2/3 complex inhibition, suggesting that actin filament remodeling is both upstream and downstream of YAP activity. These new findings on the cell biology of hESCs reveal a mechanism for cytoskeletal dynamics coordinating cell mechanics to regulate gene expression and facilitate transitions between pluripotency states.

    1. Cell Biology
    Xiaojiao Hua, Chen Zhao ... Yan Zhou
    Research Article

    The β-catenin-dependent canonical Wnt signaling is pivotal in organ development, tissue homeostasis, and cancer. Here, we identified an upstream enhancer of Ctnnb1 – the coding gene for β-catenin, named ieCtnnb1 (intestinal enhancer of Ctnnb1), which is crucial for intestinal homeostasis. ieCtnnb1 is predominantly active in the base of small intestinal crypts and throughout the epithelia of large intestine. Knockout of ieCtnnb1 led to a reduction in Ctnnb1 transcription, compromising the canonical Wnt signaling in intestinal crypts. Single-cell sequencing revealed that ieCtnnb1 knockout altered epithelial compositions and potentially compromised functions of small intestinal crypts. While deletion of ieCtnnb1 hampered epithelial turnovers in physiologic conditions, it prevented occurrence and progression of Wnt/β-catenin-driven colorectal cancers. Human ieCTNNB1 drove reporter gene expression in a pattern highly similar to mouse ieCtnnb1. ieCTNNB1 contains a single-nucleotide polymorphism associated with CTNNB1 expression levels in human gastrointestinal epithelia. The enhancer activity of ieCTNNB1 in colorectal cancer tissues was stronger than that in adjacent normal tissues. HNF4α and phosphorylated CREB1 were identified as key trans-factors binding to ieCTNNB1 and regulating CTNNB1 transcription. Together, these findings unveil an enhancer-dependent mechanism controlling the dosage of Wnt signaling and homeostasis in intestinal epithelia.