Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation

  1. Alex F Thompson
  2. Patrick R Blackburn
  3. Noah S Arons
  4. Sarah N Stevens
  5. Dusica Babovic-Vuksanovic
  6. Jane B Lian
  7. Eric W Klee
  8. Jason Stumpff  Is a corresponding author
  1. University of Vermont, United States
  2. Mayo Clinic, United States

Abstract

The chromokinesin KIF22 generates forces that contribute to mitotic chromosome congression and alignment. Mutations in the a2 helix of the motor domain of KIF22 have been identified in patients with abnormal skeletal development, and we report the identification of a patient with a novel mutation in the KIF22 tail. We demonstrate that pathogenic mutations do not result in a loss of KIF22's functions in early mitosis. Instead, mutations disrupt chromosome segregation in anaphase, resulting in reduced proliferation, abnormal daughter cell nuclear morphology, and, in a subset of cells, cytokinesis failure. This phenotype could be explained by a failure of KIF22 to inactivate in anaphase. Consistent with this model, constitutive activation of the motor via a known site of phosphoregulation in the tail phenocopied the effects of pathogenic mutations. These results suggest the motor domain a2 helix may be an important site for regulation of KIF22 activity at the metaphase to anaphase transition. In support of this conclusion, mimicking phosphorylation of a2 helix residue T158 also prevents inactivation of KIF22 in anaphase. These findings demonstrate the importance of both the head and tail of the motor in regulating the activity of KIF22 and offer insight into the cellular consequences of preventing KIF22 inactivation and disrupting force balance in anaphase.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files. Source Data files have been provided for Figure 2, Figure 2- Figure Supplement 1, Figure 3, Figure 4, Figure 4- Figure Supplement 1, Figure 5, Figure 6, Figure 6- Figure Supplement 1, Figure 7, Figure 8, Figure 8- Figure Supplement 1, Figure 9, Figure 9- Figure Supplement 1, Figure 9- Figure Supplement 2, and Figure 9- Figure Supplement 3.

Article and author information

Author details

  1. Alex F Thompson

    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Patrick R Blackburn

    Laboratory Medicine and Pathology, Mayo Clinic, Rochester, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0658-1275
  3. Noah S Arons

    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Sarah N Stevens

    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Dusica Babovic-Vuksanovic

    Laboratory Medicine and Pathology, Mayo Clinic, Rochester, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Jane B Lian

    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States
    Competing interests
    The authors declare that no competing interests exist.
  7. Eric W Klee

    Biomedical Informatics, Mayo Clinic, Rochester, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2946-5795
  8. Jason Stumpff

    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States
    For correspondence
    jstumpff@uvm.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0392-1254

Funding

National Institutes of Health (F31AR074887)

  • Alex F Thompson

National Institutes of Health (R01GM130556)

  • Jason Stumpff

National Institutes of Health (R01GM121491)

  • Jason Stumpff

National Institutes of Health (R35GM144133)

  • Jason Stumpff

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Julie P I Welburn, University of Edinburgh, United Kingdom

Publication history

  1. Received: March 15, 2022
  2. Accepted: June 21, 2022
  3. Accepted Manuscript published: June 22, 2022 (version 1)

Copyright

© 2022, Thompson et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Alex F Thompson
  2. Patrick R Blackburn
  3. Noah S Arons
  4. Sarah N Stevens
  5. Dusica Babovic-Vuksanovic
  6. Jane B Lian
  7. Eric W Klee
  8. Jason Stumpff
(2022)
Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation
eLife 11:e78653.
https://doi.org/10.7554/eLife.78653

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