Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface
Abstract
Infections at the maternal-fetal interface can directly harm the fetus and induce complications that adversely impact pregnancy outcomes. Innate immune signaling by both fetal-derived placental trophoblasts and the maternal decidua must provide antimicrobial defenses at this critical interface without compromising its integrity. Here, we developed matched trophoblast (TO) and decidua organoids (DO) from human placentas to define the relative contributions of these cells to antiviral defenses at the maternal-fetal interface. We demonstrate that TO and DO basally secrete distinct immunomodulatory factors, including the constitutive release of the antiviral type III interferon IFN-λ2 from TOs, and differentially respond to viral infections through the induction of organoid-specific factors. Finally, we define the differential susceptibility and innate immune signaling of TO and DO to human cytomegalovirus (HCMV) and develop a co-culture model of TO and DO which showed that trophoblast-derived factors protect decidual cells from HCMV infection. Our findings establish matched TO and DO as ex vivo models to study vertically transmitted infections and highlight differences in innate immune signaling by fetal-derived trophoblasts and the maternal decidua.
Editor's evaluation
Yang et al., provide a scientifically sound and compelling manuscript characterizing mid-to-late gestation trophoblast and decidual organoids as ex vivo models to study vertically transmitted microbial infections, using human cytomegalovirus as a model pathogen. They demonstrate organoids have tissue-specific immunological responses and susceptibilities to viral infection.
https://doi.org/10.7554/eLife.79794.sa0Introduction
The maternal-fetal interface is comprised of both the maternal-derived decidua and the fetal-derived placenta, which together support fetal growth, maintain maternal-fetal immunotolerance, and provide immunologic defenses to protect the fetus from infections. The relative contributions of maternal- and fetal-derived cells to these critical functions remain largely undefined given the limitations of models that recapitulate the complex nature of the human maternal-fetal interface.
The fetal-derived placenta contains distinct trophoblast subsets throughout gestation. Trophoblast stem cells located in chorionic villi, the branch-like projections that directly contact maternal blood, give rise to cytotrophoblasts (CTBs), the proliferative mononuclear cells of the placenta, the syncytiotrophoblast (STB), a multinucleated contiguous cell layer that covers the villous surfaces, and extravillous trophoblasts (EVTs). EVTs remodel the maternal microvasculature to form spiral arteries, which ultimately deliver maternal blood to the surface of chorionic villi. On the maternal side, decidualization of the endometrium is required for implantation and appropriate placentation. Taken together, the decidua and placenta maintain immunotolerance throughout gestation and provide the fetus with essential hormones and nutrients to support its growth.
While the placenta and decidua play complementary roles in fetal development, their contributions to antimicrobial defenses are less defined (reviewed in Megli et al., 2021; Semmes and Coyne, 2022). The placenta forms a powerful barrier to infections, yet certain congenital pathogens (e.g., Toxoplasma gondii, Zika virus (ZIKV), HIV, Rubella, and human cytomegalovirus [HCMV]) can be vertically transmitted to infect the fetus and in some cases, cause disease and/or pregnancy loss. Studies of tissue explants and histopathologic analysis of the placenta suggest that the decidua and chorionic villi have differing roles in the pathogenesis of vertically transmitted infections. The decidua may be a primary site of replication for several microorganisms associated with congenital disease, including HCMV (McDonagh et al., 2006; Pereira et al., 2003; Weisblum et al., 2015), ZIKV (Guzeloglu-Kayisli et al., 2020; Platt et al., 2018; Weisblum et al., 2017), and Listeria monocytogenes (Rizzuto et al., 2017), suggesting it may serve as a reservoir for these infections. By contrast, the placenta is largely resistant to infections and possesses intrinsic mechanisms of innate immune defense. For example, human trophoblasts constitutively release antiviral interferons (IFNs) that restrict infection in both autocrine and paracrine manners (Bayer et al., 2016; Corry et al., 2017). These IFNs are of the type III class, which include IFNs-λ1–3 in humans (reviewed in Lazear et al., 2019; Wells and Coyne, 2018). Constitutive release of IFNs is a unique feature of trophoblasts as IFNs are tightly regulated in most cell types and induced only after detection of the products of viral replication (reviewed in Lazear et al., 2019). Taken together, these findings suggest that maternal- and fetal-derived tissue differentially respond to and control viral infections; however, the lack of available models to fully interrogate the differences between maternal- and fetal-derived cells have limited a complete understanding of their respective roles in antiviral defense.
The paucity of tractable models to study the human maternal-fetal interface and the limitation of accessing this interface in vivo without disrupting pregnancy has limited our understanding of the pathogenesis of and immune responses against many congenital pathogens, including HCMV. HCMV is a species-specific β-herpesvirus that can only infect humans, and animal models using guinea pig (GPCMV) or rhesus macaque (RhCMV) cytomegaloviruses rely on viruses that are genetically disparate from HCMV and do not fully recapitulate the human maternal-fetal interface (Roark et al., 2020). Prior studies on placental HCMV infection have used trophoblast progenitor cells, EVT cell lines, and primary trophoblasts; however, these cell lines and isolated primary cells do not model the cellular complexity of the human placenta (Njue et al., 2020). Placental and decidual explants have also been employed to model HCMV infection, yet these human explants have high inter-individual variability, which hinders reproducibility, moreover, there is a limited window of viability in cultured ex vivo tissue (Fisher et al., 2000; Weisblum et al., 2017; Weisblum et al., 2015). Therefore, novel models of the human maternal-fetal interface are needed to understand the contributions of maternal decidual cells and fetal-derived placental cells to immune defense against and pathogenesis of placental HCMV infection.
The recent development of trophoblast (TO) and decidua organoids (DO) from first trimester placental tissue has greatly expanded the models available to study the human maternal-fetal interface (Turco et al., 2017a; Turco et al., 2018). Previous work has shown that isolation and propagation of trophoblast stem cells from first trimester placental chorionic villi lead to the development of three-dimensional organoids that differentiate to contain all lineages of trophoblast cells present in the human placenta and release well-known pregnancy hormones such as human chorionic gonadotropin (hCG) (Turco et al., 2018). Two-dimensional and organoid-based models of first trimester stem cells derived from villous tissue have also been reported and recapitulate key aspects of trophoblast biology (Karvas et al., 2022; Okae et al., 2018; Shannon et al., 2017; Sheridan et al., 2021). Similarly, DOs isolated from uterine glands recapitulate the transcriptional profile of their tissue of origin and respond to hormonal cues (Turco et al., 2017a). These organoid models are ideal to interrogate differences between maternal- and fetal-derived cells, but matched DO and TOs have not yet been used to model congenital infections.
To directly compare antiviral signaling pathways between the human placenta and decidua, we generated TO and DO from matched mid-to-late gestation (26–41 weeks of gestation) human placentas and profiled their release of cytokines, chemokines, and other factors at baseline and under virally infected conditions. These studies revealed that TOs basally secrete IFN-λ2, which does not occur in matched DOs. To determine whether maternal- and fetal-derived cells respond differently to viral infections, we compared the transcriptional profiles and immunological secretomes from organoids treated with a ligand to stimulate toll-like receptor 3 (TLR3) signaling. TO and DO differentially responded to TLR3 activation through the secretion of organoid-specific cytokines and chemokines. Additionally, we defined the differential susceptibility of trophoblast and decidua organoids to HCMV infection and identified key differences in the innate immune responses of these organoids to HCMV infection. Finally, to simulate the communication between trophoblasts and the decidua epithelium in vivo, we established a co-culture system between matched TO and DO, which showed that fetal-derived trophoblasts protect the decidual epithelium from HCMV infection. Taken together, these studies highlight the differential responses of decidual and placental cells at the maternal-fetal interface to viral infections and provide new models to understand innate immune responses against placental infections.
Results
Establishment and characterization of human trophoblast and decidual organoids from mid-to-late gestation tissue
To generate matched human TOs and decidual organoids (DOs), we used human placental tissue isolated from the second and third trimesters of human pregnancy (26–41 weeks gestation) and cultured progenitor cells isolated from chorionic villi or uterine glands of the decidua (Figure 1A). Given that previous work developed TOs utilizing tissue isolated from the first trimester (Haider et al., 2018; Marinić et al., 2020; Okae et al., 2018; Sheridan et al., 2021; Turco et al., 2017a; Turco et al., 2018), we optimized the isolation and propagation procedures for isolation of cells from mid-to-late gestation chorionic villi. This optimization included extended mechanical disruption of tissue and the addition of supplements including nicotinamide to promote progenitor cell differentiation. Although the abundance of trophoblast stem/progenitor cells decreases after the first trimester (Turco et al., 2018), we successfully isolated progenitor cells and generated organoids from all placental tissue harvested (15 unique placentas). Consistent with the presence of progenitor cells in full-term chorionic villi, we identified cells positive for the proliferation marker Ki67 in villi isolated from two late gestation placental villi used to generate TOs (Figure 1—figure supplement 1A). Isolated progenitor cells were embedded in Matrigel to allow for self-organization and propagated for ~2–3 weeks to generate organoid structures that were morphologically like those previously isolated from first trimester placentas (Turco et al., 2017a; Turco et al., 2018; Figure 1B, Figure 1—figure supplement 1B). Once established, organoids could be continuously expanded and passaged every 3–5 days for DOs and 5–7 days for TOs and were highly proliferative based on Ki67 immunostaining (Figure 1—figure supplement 1C). Confocal microscopy for cytoskeletal and adhesion markers including cytokeratin-19, actin, E-cadherin, and the Epithelial Cell Adhesion Molecule (EpCAM) confirmed the three-dimensional architecture of established organoids and verified that TOs and DOs expressed markers consistently with their tissues of origin (Figure 1C and D and Figure 1—figure supplement 1D). This also included the expression of the trophoblast-specific marker Sialic Acid Binding Ig Like Lectin 6 (SIGLEC6) in TOs but not DOs and the expression of the mucin MUC5AC in DOs but not TOs (Figure 1C and D and Figure 1—figure supplement 1D). TOs also expressed high levels of Syndecan-1 (SDC-1), a specific marker of the fused STB (Figure 1C). The localization of SDC-1 in TOs shows that like organoids derived from first trimester placental tissue (Turco et al., 2018), the apical surface of the STB is inward facing (Figure 1—video 1). We also found that TOs, but not DOs, secreted the trophoblast-specific hormone hCG as assessed by Luminex assays and over the counter (OTC) test strips (Figure 1E). The levels of hCG were stable in TOs propagated for extended periods of time (Figure 1F). In addition, we confirmed that DOs released high levels of the secreted mucin MUC16 as determined by Luminex assays, which was not present in TOs (Figure 1G).
Trophoblast stem cells and trophoblast-derived organoids derived from the first trimester placental tissue can be differentiated to an EVT-enriched phenotype through a two-step process that includes treatment with Neuregulin-1 (NRG1) (Okae et al., 2018; Sheridan et al., 2021). Similarly, we found that this differentiation process significantly increased the presence of HLA-G positive cells in TOs isolated from full-term tissue (from ~18% positive cells to ~96% positive cells) (Figure 1H) and were morphologically like those described previously (Figure 1—figure supplement 1E; Sheridan et al., 2021). Consistent with an enrichment of HLA-G positive EVTs, we detected elevated levels of matrix metalloproteinase-2 (MMP-2), which is expressed at high levels in first trimester EVTs (Isaka et al., 2003; Shimonovitz et al., 1994), in media collected from EVT differentiated TOs (Figure 1I).
Collectively, these data show that TOs and DOs are morphologically and functionally distinct and that they recapitulate human placental trophoblasts and decidua glandular epithelial cells.
Distinct transcriptional profiles in TOs and DOs
Next, we performed transcriptional profiling of established TO and DO lines using bulk RNASeq. We found that TOs and DOs were transcriptionally distinct based on differential expression analysis using DESeq2 followed by principal component analysis (PCA) (Figure 2A, Figure 2—figure supplement 1A B, C). TOs highly expressed transcripts specifically enriched in the placenta, including HSD3B1, XAGE2 and 3, GATA3, and ERVW-1, which were absent or expressed at very low levels in DOs (Figure 2B, Figure 2—figure supplement 1C and D, E). In addition, TOs highly expressed transcripts for PSGs, ERVW-1, and CGA, which were not expressed in DOs (Figure 2B). The levels of expression of these transcripts were comparable to those in primary human trophoblasts (PHTs), confirming that TOs recapitulate the transcriptional signature of primary trophoblasts (Figure 2B). These markers were consistent with the presence of the STB (e.g., CGA, PSGs, and HSD3B1), CTBs (e.g., TP63), and EVTs (e.g., ITGA5) (Figure 2B). In contrast, DOs expressed markers associated with the decidua and decidual stromal cells, including MUC1, MUC5A, MUC5B, SOX17, and HOXB8 which were absent or expressed at low levels in TOs and PHT cells (Figure 2B, Figure 2—figure supplement 1F and G). To further confirm that TOs and DOs recapitulated their tissues of origin, we profiled the expression of three members of the chromosome 19 microRNA family (C19MC), which are specifically expressed in the human placenta (Mouillet et al., 2015). We found that miR-516B-5p, miR-517A-3p, and miR-525-5p were expressed at high levels in TOs, comparable to levels observed in freshly isolated chorionic villi, but were not expressed in DOs (Figure 2—figure supplement 1I). Finally, we found that TOs isolated from placentas obtained from male fetuses expressed Y-linked genes, which were absent in female-only derived DOs (Figure 2—figure supplement 1H).
To determine if TOs derived from full-term tissue recapitulated the transcriptional signature of placental tissue throughout gestation, we performed comparative transcriptional profiling of TOs and publicly available RNASeq data sets from placental tissue isolated from the first and second trimesters, or from full-term tissue. We performed differential expression analysis of these data sets and compared the expression profiles of key pregnancy hormones and other factors associated with placental structure and function. Hierarchical clustering showed that TOs most closely clustered with samples derived from first trimester placental tissue (Figure 2C). In addition, TOs expressed high levels of pregnancy hormones such as CGA and various CGBs similar to first trimester tissue and also expressed factors including kisspeptin-10 (KISS1), which is expressed at higher levels in first trimester tissue compared to full-term tissue (Bilban et al., 2004; Figure 2C).
Collectively, these data establish matched TOs and DOs as model systems of the maternal-fetal interface and demonstrate that they can be derived from tissue after the first trimester, including from mid-to-late gestation. Furthermore, these data suggest that TOs derived from full-term tissue recapitulate the expression profile of first trimester placental tissue.
Comparative immune secretome profiling of TOs and DOs
We have shown previously that human chorionic villous explants release numerous cytokines, chemokines, and growth factors that impact both maternal and fetal antimicrobial defenses (Corry et al., 2017; Megli et al., 2021). Similarly, we have shown that PHTs isolated from mid-gestation and full-term placentas secrete some of these same immunomodulatory factors (Corry et al., 2017; Megli et al., 2021). We therefore profiled the immunological secretome of matched TOs and DOs isolated from independent placentas to define basal differences in the secretome of cells that comprise the maternal-fetal interface. To do this, we used multianalyte Luminex-based profiling of 105 cytokines, chemokines, growth, and other factors from conditioned media (CM) isolated under basal unstimulated conditions. Factors whose secretion was reliably measured over 50 pg/mL were defined as present in CM. We found that TOs secreted only two cytokines (IL-6 and IL-28A/IFN-λ2) and three immune-regulated secreted soluble receptors (sTNF-R1, gp130/sIL-6Rβ, and sTNF-R2) at baseline (Figure 3A). By contrast, DOs released a larger number of immune factors overall, with 21 detected in DO-derived CM at baseline (Figure 3B). PCA of these Luminex profiles identified factors contributing to the differential nature of the secretome between TOs and DOs, which included the enrichment of factors including CXCL1, CXCL5, and IL-8 in DOs versus IL-6 and IL28A/IFN-λ2 in TOs (Figure 3C–F). Taken together, these data define the unique immunologic secretome of key cells that comprise the maternal-fetal interface and show that tissue-derived organoids recapitulate some of the secretory phenotypes observed in tissue explants and/or primary cells.
TOs constitutively secrete antiviral IFN-λ2
We showed previously that PHT cells isolated from full-term placentas and mid-gestation chorionic villi constitutively release type III IFNs, which protect trophoblasts from viral infections (Bayer et al., 2016; Corry et al., 2017). We found that TOs recapitulated this phenotype and released high levels of IFN-λ2, but not type I IFNs or IFN-λ1 (Figure 4A). In contrast, DOs did not basally secrete any detectable IFNs (Figure 4B). The levels of released IFN-λ2 were comparable to those from PHT cells and from matched cultured villous explants (Figure 4C). We monitored the release of IFN-λ2 over the growth period of TOs and found that its release was detectable by ~10 days post-plating and reached high levels by >14 days in culture, which correlated with similar trends for IL-6 (Figure 3—figure supplement 1). We confirmed that CM isolated from TOs, but not DOs, induced interferon-s (ISGs) in cells treated with this CM using a HEK293-based reporter assay (Figure 4D). Consistent with this, CM isolated from TOs was antiviral and reduced ZIKV infection in CM-treated non-placental cells including U2OS cells (Figure 4E) and in DOs (Figure 4F and G), confirming that TO-derived CM exerts potent antiviral effects on non-placental cells. Finally, we found that TOs differentiated into an EVT-enriched phenotype exhibited significantly reduced levels of secreted IFN-λ2, suggesting that EVTs are not the primary producers of IFN-λ2 in TOs (Figure 4H).
Differential induction of cytokine and chemokine networks in TOs and DOs in response to poly I:C treatment
Because we observed differences in the basal secretomes of TOs and DOs, we next determined whether TOs and DOs differentially respond to viral infections. To model RNA virus infection, we used poly (I:C), a synthetic ligand of TLR3, and profiled the transcriptional and secretory changes induced by poly I:C treatment of TOs and DOs. We found that both TOs and DOs robustly responded to poly I:C treatment, which corresponded with significant transcriptional changes as assessed by RNASeq (Figure 5A and B). Comparison of the transcripts induced by poly I:C treatment (as defined by significance of p<0.01 and log2 fold-change of ±2) of TOs and DOs revealed that TOs upregulated 448 transcripts whereas DOs upregulated 121 (Figure 5—figure supplement 1A). Of these, only 69 were shared between TOs and DOs, the majority of which (64 of 69) were ISGs (Figure 5—figure supplement 1A and B). Of the differentially induced transcripts, TOs induced the expression of chemokines including CXCL5 (log2 fold change 5.9), CX3CL1 (log2 fold change 7.1), and CCL22 (log2 fold change 7.6), which were the most induced cytokine or chemokine transcripts in poly I:C-treated TOs (Figure 5C). In contrast, DOs expressed chemokines at high basal levels, which were not further induced by poly I:C treatment and did not induce any CCL22 (Figure 5C). Transcripts most selectively induced in DOs included CD38 and the phospholipase PLA1A (Figure 5C).
To confirm the transcriptional changes described above, we performed parallel multianalyte Luminex profiling of 105 cytokines and chemokines in TOs and DOs treated with poly I:C. Both TOs and DOs mounted robust responses to poly I:C treatment that correlated with the release of high levels of select cytokines and chemokines (Figure 5D). Cytokines and chemokines with levels >5-fold above baseline were defined as induced by poly I:C treatment. The topmost induced factors in poly I:C-treated TOs were the chemokines CCL22, CXCL5, CXCL2, and CX3CL1 (Figure 5D, left), which was consistent with their transcriptional upregulation by RNASeq (Figure 5C). PCA of Luminex-based profiling of poly I:C treated TOs and DOs revealed organoid-specific responses to this treatment, which included the specific induction of CCL22 in poly I:C treated TOs (Figure 5—figure supplement 1C, Figure 5E and F). Factors induced by both TOs and DOs included the type III IFNs IFN-λ1 and IFN-λ2 (Figure 5G and H), which were the topmost induced factors in DOs. In contrast, the type I IFNs IFN-α2 and IFNβ were induced to significantly greater levels in TOs than DOs (Figure 5—figure supplement 1D and E). Other chemokines induced in TOs were basally expressed at high levels in DOs and were not further induced by poly I:C (Figure 5—figure supplement 1F-J).
Differential susceptibility of TOs and DOs to HCMV infection
HCMV is the most common vertically transmitted pathogen and is associated with high rates of placental dysfunction, pregnancy loss, and congenital disease. However, how HCMV transmits across the placenta and how the maternal decidua and fetal-derived placenta respond to HCMV infection remains poorly defined. To address these questions, we infected TOs and DOs with HCMV strains AD169r (BADrUL131-Y4, tagged with GFP; Wang and Shenk, 2005) and TB40E (tagged with mCherry; O’Connor and Shenk, 2011). We found that DOs were highly permissive to HCMV infection as quantified by detectable GFP or mCherry expression of viral gene expression in most organoids (Figure 6A, C and D). In contrast, TOs were largely resistant to HCMV infection, with low levels of infection by AD169r and almost undetectable levels by TB40E, as assessed by fluorescence of tagged viral gene expression (Figure 6B, C and D). Consistent with this, analysis of the expression of key HCMV products using bulk RNASeq from infected TOs and DOs showed that the HCMV-associated factors RNA4.9, which is important for HCMV DNA replication (Tai-Schmiedel et al., 2020), and UL63 were significantly lower in TOs compared to DOs (Figure 6E).
Because we observed minimal, but detectable, HCMV infection in TOs, we next sought to determine whether the low levels of HCMV infection we observed occurred in EVTs, which have been proposed as targets of many teratogenic viruses including HCMV (reviewed in Megli et al., 2021). To do this, we infected TOs cultured under standard conditions or under conditions that induced their differentiation into EVTs. Despite the significant enrichment of HLA-G positive EVTs under differentiated conditions, we found that there was no significant enhancement of HCMV infection under these conditions (Figure 6F and G), suggesting that EVTs are not the primary targets of HCMV infection in TOs.
Differential transcriptional responses of TOs and DOs to HCMV infection
Because we observed significant differences between the susceptibility of TOs and DOs to HCMV infection with strains AD169r and TB40E, we next profiled the transcriptional changes induced by HCMV infection in these organoids. Matched TOs and DOs were infected with AD169r or TB40E and the transcriptional impact of infection was assessed by bulk RNASeq transcriptional profiling. TOs responded to HCMV infection through the differential expression of 282 transcripts (AD169r strain) or 173 transcripts (TB40E strain), of which ~38% were shared between both strains of HCMV (Figure 7A and C). By contrast, DOs responded to HCMV infection less robustly, with 95 (AD169r strain) or 117 (TB40E strain) transcripts differentially expressed by this infection (Figure 7B and D). Of these differentially expressed transcripts, only 3–4% were shared between TOs and DOs infected with AD169r (Figure 7E) or TB40E (Figure 7F). Of these shared transcripts, only three were induced by both strains of HCMV in both TOs and DOs and included CCNA1 (Cyclin A1), the ISG OAS2, and EPSTI1 (Epithelial Stromal Interaction 1) (Figure 7G). Transcripts specifically induced by HCMV infection of TOs included H3Y1 (H3.Y Histone 1), KHDC1L (KH Domain Containing 1 Like), several members of the PRAME Family (PRAMEF2, 8, 12, 14, and 20), and TRIM49A and B (Tripartite Motif Containing 43) (Figure 7H). In contrast, DO-specific transcripts included several components of the nitric oxide signaling pathway including NOS2 (Nitric Oxide Synthase 2), DUOX2 (Dual Oxidase 2), and DUOXA2 (Dual Oxidase Maturation Factor 2) as well as CXCL10 and CSF3/G-CSF (Figure 7I).
Differential immune secretome of TOs and DOs infected with HCMV
To define the innate immune responses to HCMV infection in TOs and DOs, we used Luminex multianalyte profiling of >100 cytokines and chemokines in TOs and DOs infected with HCMV strains AD169r or TB40E. We found that TOs secreted relatively few cytokines in response to HCMV infection, including pentraxin-3 (PTX3), first described as an endothelial factor induced by IL-1β treatment (Breviario et al., 1992), IL-8, and IL-11 (Figure 8A, C and D, Figure 8—figure supplement 2A). In contrast, DOs responded to HCMV infection by secreting both proinflammatory cytokines and chemokines (e.g., CXCL10, MIP-1α, MIP-1β, and IL-6) and through the release of the type III IFN IFN-λ2 (Figure 8B, C and E, Figure 8—figure supplement 2B). In contrast to IFN-λ2, DOs did not induce any other detectable IFNs such as IFN-α2 or IFN-β or IFN- λ1 (Figure 8—figure supplement 2C-E).
Because we observed high levels of basal IFN-λ2 secretion in TOs, which were resistant to HCMV infection, and high induction of IFN-λ2 in DOs infected with HCMV, we next determined whether inhibiting this IFN signaling would alter the sensitivity of DOs or TOs to HCMV infection. To test this, we used the JAK-specific inhibitor ruxolitinib, which inhibits signaling downstream of type III IFN receptor activation. DOs and TOs were treated with ruxolitinib for 1 hr prior to infection with the AD169r strain of HCMV for 48 hr in the presence of ruxolitinib. We found that treatment of DOs with ruxolitinib significantly increased levels of HCMV replication, which correlated with higher levels of GFP fluorescence (Figure 8F and G). In contrast, treatment of TOs with ruxolitinib did not increase levels of HCMV replication over DMSO treated controls, which remained low under both ruxolitinib-treated and DMSO-treated conditions (Figure 8G).
To model communication between the maternal-fetal interface and to define this impact of this communication on HCMV infection, we established a co-culture model of TOs and DOs developed from matched placental tissue. To do this, equivalent numbers of TOs and DOs were pre-mixed prior to culturing and co-cultured for ~5 days prior to infection with HCMV. We confirmed that co-cultured organoids contained both TOs and DOs and distinguished between these organoids using SIGLEC-6 immunostaining, with only TOs positive for this marker (Figure 8H). Following the establishment of co-cultures, we infected DOs or TOs cultured alone or co-cultures and TOs and DOs with HCMV and determined the impact of this culturing on infection. We found that whereas singly cultured DOs were permissive to HCMV infection, co-culturing of DOs and TOs led to a significant reduction in HCMV infection in DOs (Figure 8I and J).
Taken together, these findings highlight the distinct immunoregulatory and transcriptional cascades induced by HCMV infection of maternal-derived decidual versus fetal-derived placental cells and suggest that IFNs restrict HCMV infection in decidual epithelial cells. In addition, our co-culture studies support a model whereby TO-derived factors confer protection of epithelial cells in the decidua from HCMV infection.
Discussion
Our work establishes fetal- and maternal-derived organoids as models for defining innate immune signaling at the maternal-fetal interface. Similar to PHTs (Bayer et al., 2016) and mid-gestation chorionic villi (Corry et al., 2017), we show that TOs constitutively secrete type III antiviral IFN-λ, that act in paracrine to restrict viral infections. In contrast, although DOs did not basally secrete IFNs, they are immunologically more active under resting conditions and basally secrete cytokines and chemokines not detectable in TOs. In addition, we show that TOs and DOs respond to TLR3 activation via the induction of distinct immune regulatory networks, which includes the specific release of select chemokines such as CCL22 in TOs. Finally, we used matched TOs and DOs to explore differences in susceptibility to HCMV and to define the differential responses of the maternal decidua and fetal-derived organoids to HCMV infection. Taken together, our work suggests that cells that comprise the maternal-fetal interface defend from viral infections in a cell-type-specific manner and further highlight the unique contributions of fetal-derived trophoblasts in antiviral immunity.
The previous establishment of trophoblast and decidua-derived organoids has significantly expanded the in vitro systems that can model the complexity of the maternal-fetal interface (Turco et al., 2017a; Turco et al., 2018). These previous studies utilized tissue isolated from the first trimester of pregnancy (6–9 weeks of gestation for TOs and 8–12 weeks of gestation for DOs) (Turco et al., 2017a; Turco et al., 2018), although another study successfully established DOs from full-term tissue (Marinić et al., 2020). Two-dimensional cultures of stem cell-derived primary trophoblasts were also obtained from the first trimester (Okae et al., 2018). The placenta is a dynamic organ that changes throughout gestation. Here, we show that TOs can also be generated from later stages of gestation, including from progenitor cells isolated in the second and third trimesters of pregnancy and full-term placental tissue, which required optimization from conditions required for first trimester tissue. Although the abundance of progenitor cells, as defined by the presence of Ki67 positive nuclei, declines throughout gestation, we show that full-term chorionic villi contain Ki67 positive cells at low levels and that these cells can be isolated to generate self-propagating organoids. Given that obtaining first trimester tissue can be complicated by access and/or regulatory restrictions, our study provides an additional source for the development of placental organoids from tissue obtained later in gestation. In addition, given that many congenital infections occur in the second and third trimesters of pregnancy, organoids isolated from later stages of pregnancy may better reflect clinically relevant models of congenital infections. For instance, the risk of transplacental transmission of HCMV infection from mother to fetus is only 20% in the first trimester but can reach up to 75% in the third trimester, highlighting the relevance of our organoid model for studying placental HCMV infection across gestation (Pass and Anderson, 2014).
Unlike many other barrier cell types such as the epithelium, human trophoblasts have the unique capacity to robustly secrete cytokines and other immunological factors without stimulation. PHTs from full-term placentas and mid-gestation chorionic villi secrete cytokines that mediate innate immune defenses against bacteria, parasites, and viruses (Ander et al., 2018; Bayer et al., 2016; Megli et al., 2021). Consistent with this concept, serum isolated from pregnant women often contains elevated levels of these cytokines, indicating that at least some have reached the systemic circulation (Benyo et al., 2001; Liu et al., 2019; Megli et al., 2021; Vassiliadis et al., 1998). Similarly, the decidua releases specific cytokines and alterations in these profiles may be associated with pregnancy complications including miscarriage (Alam et al., 2008; Denison et al., 1998; Laham et al., 1997; Lash and Ernerudh, 2015; Pitman et al., 2013; von Rango et al., 2003). The decidua also responds to microbial infections via the release of additional cytokines that function in innate defense (Barber et al., 2005; Guzeloglu-Kayisli et al., 2020; Qiu et al., 2009). However, in many cases, these studies were performed on tissue explants that were multicellular in nature, thus preventing an assessment of the contributions of cytokine release from distinct cell types. Similarly, previous work utilizing tissue explants that were treated with recombinant cytokines, synthetic ligands of various innate immune pathways, or infected with pathogens are also unable to define the contributions of distinct cell types in these responses. The use of organoids allows for analyses of cellular responses to infection or innate immune activation from trophoblasts and decidual glands in primary cell models that contain isolated cell types enriched at the maternal-fetal interface. An additional strength of the TO model is that trophoblast stem cells can differentiate into all three distinct trophoblast cell types comprising the human placenta, which is not possible in many other existing models including isolation of CTBs from full-term placentas, which do not differentiate to contain EVTs.
The constitutive release of IFN-λs from human trophoblasts is a unique phenomenon that runs counter to much of what is known about antiviral IFN signaling, which is centered on a requirement for viral sensing as a prerequisite to induce this pathway. Similar to our previous studies in PHT cells and mid-gestation chorionic villous explants (Bayer et al., 2016; Corry et al., 2017), our data show that progenitor cell-derived TOs also constitutively release IFN-λs and protect the epithelium of the decidua from viral infections. Our studies also show that type III IFNs are further induced by activation of antiviral innate immune signaling in both TOs and DOs, with IFN-λs being amongst the most induced cytokines detected in both organoid types. Although IFNs are released from TOs constitutively and upon induction of antiviral signaling, our studies also point to select chemokines as key components in the responses of the human placenta to viral infections. We show that the regulatory T cell (Treg) chemoattractant CCL22 is selectively released from TOs in response to TLR3 activation. We have shown previously that human PHTs and mid-gestation chorionic villi also respond to T. gondii infection through the selective release of CCL22 (Ander et al., 2018; Rudzki et al., 2021), suggesting that this chemokine may be key mediator of the response of the human placenta to microbial infections. Given that Treg infiltration into the decidua has been associated with adverse pregnancy outcomes including miscarriage (Freier et al., 2015), CCL22 induction may directly impact maternal-fetal tolerance in the context of congenital infection.
Significant work over many decades using first trimester placental explants and isolated human trophoblasts have revealed important insights into placental HCMV transmission and antiviral immunity (reviewed in Njue et al., 2020). Yet, a full understanding of the distinct roles of decidual versus trophoblast cells in HCMV infection has been hindered by a lack of tractable model systems of the human maternal-fetal interface. In this study, we leveraged HCMV as a model microorganism to demonstrate the utility of organoid models for studying infections at the maternal-fetal interface. Using TO and DO, we found that DOs had heightened susceptibility to HCMV infection compared to matched TOs, which is consistent with prior work in first trimester decidua explants showing that HCMV productively infects the maternal decidua (Weisblum et al., 2015; Weisblum et al., 2015). Our transcriptional and immune secretome profiling further highlights that TOs and DOs differentially respond to HCMV infection and recapitulates prior findings using isolated human placental cells and explants. CXCL10 was highly upregulated in HCMV-infected DOs, consistent with prior findings of increased CXCL10 secretion in decidua explants infected with HCMV ex vivo (Weisblum et al., 2017; Weisblum et al., 2015). IL-8 was also highly induced in HCMV-infected TOs, mirroring the increased IL-8 secretion that has been observed in response to ex vivo HCMV infection of isolated trophoblasts (Kovács et al., 2007) and placental explants (Weisblum et al., 2017). That our in vitro TO and DO infections recapitulate innate immune responses previously observed in vivo and ex vivo validates our model of matched decidua and trophoblast organoids for studying HCMV and other congenital pathogens. Moreover, our data demonstrate that type III IFNs control HCMV infection in the maternal decidua but suggest that additional factors or pathways beyond IFN signaling may restrict HCMV infection of trophoblasts. The constitutive release of type III IFNs from fetal trophoblasts has been proposed to play a direct role in the protection of maternal-derived cells from viral infections. Indeed, ablation of type III IFN signaling in the murine placenta enhances infection of ZIKV in maternal decidua tissue (Jagger et al., 2017). The co-culture model presented here also supports a model in which trophoblast-derived effectors, including IFN-λ, work in paracrine to suppress HCMV infection in cells comprising the maternal decidua. Altogether, these data highlight the utility of organoid models for understanding HCMV transmission and immune defense at the maternal-fetal interface and underscore the need for more research using organoid models to disentangle the roles of decidual versus placental cells in congenital viral infections.
Our work presented here develops TOs and DOs from mid-to-late gestation placental tissue and demonstrates that these organoids can be used to study mechanisms of microbial vertical transmission and antiviral innate immune signaling. We show that TOs and DOs constitutively secrete unique cytokines and chemokines and respond to viral infections through the release of organoid-specific immunomodulatory factors. Our work also highlights the prominent role of type III IFNs in antiviral immunity at the maternal-fetal interface and demonstrates that TOs constitutively release IFN-λs and that DOs can be induced to release IFN-λs following viral infection. Collectively, these studies define the differential responses of decidual and placental cells comprising the maternal-fetal interface to viral infections and suggest that organoid-based models can be used to define innate immune signaling at this interface.
Materials and methods
Human samples
Request a detailed protocolHuman tissue used in this study was obtained through the UPMC Magee-Womens Hospital Obstetric Maternal & Infant Database and Biobank or from Duke University after approval was received from the University of Pittsburgh or Duke University Institutional Review Board (IRB) and in accordance with the guidelines of the University of Pittsburgh and Duke University human tissue procurement. Placental tissue used in this study was collected from the second (23rd–27th weeks) and third (35th–41st weeks) trimesters. Placental tissue was excluded for diagnosis of PPROM or maternal infection.
Derivation and culture of TOs from human placental samples
Request a detailed protocolVillous trophoblast stem/progenitor cells were isolated from fresh placental tissues as described previously with further optimization based on the characteristics of late gestation placenta villi (Turco et al., 2018). Briefly, villi collected from fresh placental tissue were intensively washed, then were sequentially digested with 0.2% trypsin-250 (Alfa Aesar, J63993-09)/0.02% EDTA (Sigma-Aldrich, E9884-100G) and 1.0 mg/mL collagenase V (Sigma-Aldrich, C9263-100MG) and further mechanically disrupted by pipetting up and down vigorously ~10 times with 10 mL serological pipette. Pooled digests were washed with Advanced DMEM/F12 medium (Life Technologies 12634-010) and pelleted by centrifugation, then resuspended in approximate 10 times volume of ice-cold growth-factor-reduced Matrigel (Corning 356231). Matrigel ‘domes‘ (40 µL/well) were plated into 24-well tissue culture plates (Corning 3526), placed in a 37oC incubator to pre-polymerize for ~3 min, turned upside down to ensure equal distribution of the isolated cells in domes for another 10 min, then carefully overlaid with 500 µL prewarmed trophoblast organoid medium (TOM) consisting of Advanced DMEM/F12 (Life Technologies, 12634-010) supplemented with 1× B27 (Life Technologies, 17504-044), 1× N2 (Life Technologies, 17502-048), 10% fetal bovine serum (FBS) (vol/vol, Cytiva HyClone, SH30070.03), 2 mM GlutaMAX supplement (Life Technologies, 35050-061), 100 µg/mL Primocin (InvivoGen, ant-pm-1), 1.25 mM N-Acetyl-L-cysteine (Sigma-Aldrich, A9165), 500 nM A83-01 (Tocris, 2939), 1.5 µM CHIR99021 (Tocris, 4423), 50 ng/mL recombinant human EGF (Gibco, PHG0314), 80 ng/mL recombinant human R-spondin 1 (R&D systems, 4645-RS-100), 100 ng/mL recombinant human FGF2 (Peprotech, 100-18C), 50 ng/mL recombinant human HGF (Peprotech, 100-39), 10 mM nicotinamide (Sigma-Aldrich, N0636-100G), 5 µM Y-27632 (Sigma-Aldrich, Y0503-1MG), and 2.5 µM prostaglandin E2 (PGE2, R&D systems, 22-961-0). Cultures were maintained in a 37oC humidified incubator with 5% CO2. Medium was renewed every 2–3 days. Small trophoblast organoid spheroids became visible by approximately day 12 post-isolation. Derived TOs were passaged every 5–7 days depending on their size and density. To passage, TOs were released from domes with ice-cold cell recovery solution (Corning, 354253) and then were digested in prewarmed TrypLE Express (Life Technologies, 12605-028) or Stem Pro Accutase (Life Technologies, A11105-01) at 37oC for 5–7 min. Further mechanical dissociation was achieved with an Eppendorf Explorer Plus electronic pipettes at its ‘mix’ function setting by pipetting up and down around 400 times through small-bore pipette tips. Dissociated TOs fragment were collected and washed by centrifuge, then resuspended in fresh ice-cold Matrigel and replated as domes at the desired density for continuous culture. For freezing TOs, overlaid TOM was aspirated, TOs with Matrigel were resuspended in CryoStor CS10 stem cell freezing medium (STEMCell Technologies, 07930) frozen at –80 oC and then transferred to liquid nitrogen for long-term storage. For thawing cryopreserved TOs, organoids were thawed as quickly as possible, diluted with five times volume of basic TOM containing Advanced DMEM/F12, 2 mM GlutaMAX supplement, 10 mM HEPES (Gibco, 15630-106), 1× Penicillin/Streptomycin (Lonza, 17-602E) and centrifuged to pellet. Afterward, TOs were resuspended in new ice-cold Matrigel and replated for recovery culture and passaged as described below. Trophoblast organoids used in this study were derived from 15 independent placentas (7 male and 8 female). Detailed protocols for the isolation and passaging of trophoblast organoids derived from full-term placental tissue have been posted and are available for download (Yang and Coyne, 2022a; Yang and Coyne, 2022b).
Derivation and culture of DOs cultures from human decidua samples
Request a detailed protocolDecidual gland-enriched cell suspension was acquired from fresh decidual tissue as described previously (Turco et al., 2017b; Turco et al., 2017a). Briefly, isolated decidual tissues were cut into small pieces then digested in 1.25 U/mL Dispase II (Sigma-Aldrich, D4693)/0.4 mg/mL collagenase V (Sigma-Aldrich, C-9263) solution. Glandular cells were filtered out from the digestion solution supernatant and resuspended in ice-cold Matrigel (Corning, 356231), plated as domes in 24-well plates (Corning, 3526), and finally overlaid with 500 µL prewarmed organoid Expansion Medium (ExM) with the same composition as previously described (Turco et al., 2017a). The growth medium was renewed every 2–3 days. Mature organoids were passaged by mechanically disruption following TrypLE Express digestion every 3–5 days. Other maintenance were similar to those as previously described (Turco et al., 2017b).
Differentiation of TOs into EVT-enriched TOs
Request a detailed protocolTo generate EVT-enriched TOs, we adopted EVT differentiation protocols developed previously (Okae et al., 2018; Sheridan et al., 2021). TOs were passaged as described above and plated directly into eight-well chamber slides (Millicell, EZslide, Millipore) pre-coated with ~15 µL Matrigel. After plating, organoids were maintained in TOM as described above for 3–4 days, then switched to EVT differentiation media 1 (EVT m1: advanced DMEM/F12 supplemented with 2 mM L-glutamine, 0.1 mM 2-mercaptoethenol, 0.5% [vol/vol] penicillin/streptomycin, 0.3% [vol/vol] BSA, 1% [vol/vol] ITS-X supplement, 100 ng/mL NRG1 [Cell Signaling Technology, 5218SC]), 7.5 µM A83-01, 2.5 µM Y27632, and 4% (vol/vol) Knockout Serum Replacement (Gibco, 10828010), for 9 days. EVT m1 was renewed every 2 days for the initial 4 days then daily for the remaining culture period. Subsequently, organoids were switched to EVT m2 with the same recipe as EVT m1, but lacking NRG1 for a further 3–4 days, with daily media changes. HCMV infection of EVT-enriched TOs in eight-well chamber slide was performed as described as described below.
Co-cultures of matched TOs and DOs
Request a detailed protocolMatched lines of TOs and DOs were passaged regularly as described above. After passaging, equivalent numbers of TOs and DOs were combined, centrifuged, then resuspended with TOM supplemented with 5 µM Y27632. Mixed TOs and DOs were plated directly into eight-well chamber slides pre-coated with ~15 μL Matrigel and cultured for ~5 days prior to HCMV infection performed as described below. The presence of TOs was confirmed using OTC rapid hCG test strips, with only DOs co-cultured with TOs testing positive (Figure 8—figure supplement 1B).
Generation of TO and DO conditioned media
Request a detailed protocolOverlaid TOM and ExM were collected every 2–4 days based on the growth status of TOs and DOs. Media from at least 10 time points for each established organoid lines were collected as TO-CM or DO-CM. For TO CM, media were collected starting at ~4 days after passaging, or when the majority of organoids were 100–200 µm in size. The levels of hCG in collected CM were measured by Luminex assays or rapid hCG test strips prior to use. CM with low levels of hCG were not included in subsequent experiments. For DO CM, media were collected ~2 days after passaging, or when most organoids had formed obvious cystic structure of at least 200 µm. In most experiments, the use of TO and DO CM from matched tissue was used, although there did not appear to be any significant differences in CM from unmatched donor tissue. Non-conditioned medium (NCM) was TOM or ExM (described above) that had not been exposed to TOs or DOs, respectively. All experiments were performed with CM from at least three independent TO and DO lines.
Cells and viral infections
Request a detailed protocolHuman osteosarcoma U2OS (ATCC HTB-96) and Vero (ATCC CCL-81) cells were grown in Dulbecco’s minimal essential medium (DMEM) with 10% FBS and 1% antibiotic. HEK-Blue IFN-λ reporter cells (hkb-ifnl) were purchased from InvivoGen and cultured according to the manufacturer’s instructions. Cell lines were tested for mycoplasma at least every 2–4 weeks using the MycoStrip Mycoplasma Detection Kit (Invivogen, rep-mys-50). ZIKV (Paraiba/2015, provided by David Watkins, University of Miami) was propagated in Vero cells. Viral titers were determined by fluorescent focus assay as previously described (Payne et al., 2006) using recombinant anti-double-stranded RNA monoclonal antibody J2 (provided by Abraham Brass, University of Massachusetts) in Vero cells. Infection was determined by qRT-PCR as described below. To assess antiviral activity, U2OS cells were exposed to TO or DO CM, or matched NCM, 24 hr prior to infection with ZIKV Paraiba/2015 for 72 hr. GFP-tagged AD169r (BADrUL131-Y4, a gift from T. Shenk; Wang and Shenk, 2005) and mCherry-tagged TB40/E (a gift from N. Moorman; O’Connor and Shenk, 2011) were propagated by infecting HFF cells (ATCC SCRC-1041) (MOI=0.01) followed by incubation for ~14 days until 90–95% of cells showed cytopathic effect. Cells and supernatant were harvested, filtered (0.45 μm), and then concentrated on a 20% sucrose cushion (20,000 rpm) before the virus pellet was resuspended in sterile media before titration on HFFs and retinal epithelial ARPE-19 cells (ATCC CRL-2302). HCMV infection of TOs and DOs was performed using 3–4×10^5 PFU virus per well of an 8-well chamber slide or 1x10^6 per well of a 24-well plate for 48 hr. For infections, TOs and DOs were plated directly onto 15 μL of a Matrigel coating in each well of an 8-well chamber slide (Millicell, EZslide, Millipore) or onto 20 μL of Matrigel coating in 24-well plates and cultured for 5–7 days prior to infection. After 48 hr, CM was harvested and stored at –80°C until Luminex profiling and cells were fixed with paraformaldehyde (PFA) for 15 min before staining for immunofluorescence microscopy, as detailed below.
Immunofluorescence microscopy
Request a detailed protocolTOs and DOs were released from Matrigel domes with 1 mL of cell recovery solution (Corning, 354253) per well without disrupting their 3D architecture, then were fixed in 4% PFA for 30 min at room temperature, followed by 0.5% Triton X-100/phosphate-buffered saline [PBS] to permeabilize for 30 min at 4°C. Organoids were washed and blocked in 5% (v/v) goat serum/0.1% (v/v) Tween-20 in PBS for 15 min at room temperature and then incubated with primary antibodies in the above-described blocking solution at 4oC overnight. Organoids were then washed with PBS and then incubated for 1–2 hr at room temperature with Alexa Fluor-conjugated secondary antibodies (Invitrogen). Organoids were washed again with PBS and mounted in Vectashield (Vector Laboratories) containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL tips (Thermo Fisher Scientific, 02707134). For staining performed in eight-well chamber slides, the same protocol described above was used, but the releasing of organoids from Matrigel was omitted. The following antibodies or reagents were used: cytokeratin-19 (Abcam, ab9221 and ab52625), E-cadherin (Invitrogen, PA5-85088), EpCAM (Abcam, ab223582), Mucin 5AC (Abcam, ab3649), SIGLEC6 (Abcam, ab262851), HLA-G (Abcam, ab52454 and ab283260), Ki67 (550609, BD Biosciences), SDC-1 (Abcam, ab128936), J2 double-stranded RNA antibody as described previously (Bayer et al., 2016), Alexa Fluor 594-conjugated phalloidin (Invitrogen, A12381), Alexa Fluor 633-conjugated phalloidin (Invitrogen, A22284), Alexa Fluor Plus 488 Goat anti-Mouse IgG secondary antibody (Invitrogen, A32723), Alexa Fluor 594 Goat anti-Mouse IgG secondary antibody (Invitrogen, A11032), Alexa Fluor 488 Goat anti-Rabbit IgG secondary antibody (Invitrogen, A11034), and Alexa Fluor 594 Goat anti-Rabbit IgG secondary antibody (Invitrogen, A11037). Images were captured using Zeiss 880 Airyscan Fast Inverted confocal microscope and contrast-adjusted in Photoshop or Fiji (Schindelin et al., 2012). For whole Matrigel dome images, scans were performed using a 10× objective on an inverted IX83 Olympus microscopy with a motorized XY-stage (Prior) and tiled images automatically generated by CellSens (Olympus). Image analysis and three-dimensional image reconstruction were performed using Imaris version 9.2.1 (Oxford Instruments).
Immunohistochemistry
Request a detailed protocolTissue sections were deparaffinized with xylene and rehydrated with decreasing concentrations of ethanol (100%, 95%, and 80%), then washed with ddH20. Antigen retrieval was performed with slides submerged in 10 mM citrate buffer (pH 6.0) and heated in a steamer for 90°C for 20 min. Slides were cooled to room temperature and incubated with 6% H2O2 in methanol for 30 min. Following washing in 0.1% PBS-T (PBS, 0.1% Tween 20), slides were incubated in Avidin blocking solution for 15 min, followed by subsequent blocking in Biotin blocking solution for 15 min (Vector Laboratories, SP-2001). Following washing in 0.1% PBS-T, slides were then incubated with serum-free Protein Block (Abcam, ab156024) for 10 min. Sections were incubated with primary antibodies (cytokeratin-19; Abcam, ab9221) and Ki67 (550609, BD Biosciences) diluted 1:250 in PBS-T overnight in a humidified chamber at 4°C. Next, slides were washed with PBS-T and incubated with secondary antibody (Biotinylated Goat Anti-Rabbit or Mouse IgG, Vector Biolaboratories BA-1000 and BA-9200) for 30 min, washed, and then incubated with avidin/biotin-based peroxidase (Vector Laboratories, Vectastain Elite ABC HRP Kit, PK-6100) for an additional 30 min. Following washes in PBT-T, sections were incubated with DAB substrate (Vector Laboratories, SK-4100) for ~5 min. Slides were washed with ddH20 and then counterstained with hematoxylin for 1 min, thoroughly rinsed with H2O, and incubated in 0.1% sodium bicarbonate in H2O for 5 min. Slides were then dehydrated with increasing concentrations of ethanol, cleared with xylene, and mounted with Vectamount Permanent Mounting Medium (Vector Laboratories, H-5000). Images were captured on an IX83 inverted microscope (Olympus) using a UC90 color CCD camera (Olympus).
RNA extraction, cDNA synthesis, and quantitative PCR
Request a detailed protocolTotal RNA was extracted with the Sigma GenElute total mammalian RNA miniprep kit following the manufacturer’s instruction and using the supplementary Sigma DNase digestion. RNA quality and concentration were determined using a Nanodrop ND-1000 Spectrophotometer. Total RNA was reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s instructions. Quantitative PCR was performed using the iQ SYBR Green Supermix (Bio-Rad, 1708882) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Gene expression was determined based on a ΔCT method normalized to actin. The expression level of C19MC miRNAs hsa-miR-516b-5p, hsa-miR-517a-3p, hsa-miR-525-5p, and reference gene RNU6 was quantified by using the MystiCq MicroRNA Quantitation System (Millipore Sigma) which includes miRNAs Poly(A) tailing, cDNA synthesis (MIRRT), and miRNA qPCR (MIRRM00). MicroRNAs were first polyadenylated in a poly(A) polymerase reaction, then reverse transcribed using an oligo-dT adapter primer according to the manufacturer’s protocol (Sigma-Aldrich, MIRRT). Individual microRNAs (microRNA-516b-5p, microRNA-517a-3p, and microRNA-525-5p) were quantified in real-time SYBR Green RT-qPCR reactions with the specific MystiCq microRNA qPCR Assay Primer (MIRAP00475, MIRAP00477, and MIRAP00512) and the MystiCq Universal PCR Primer (MIRUP). Cq readings were normalized to the RNU6 internal reference by the 2-ΔCq method.
RNAseq
Request a detailed protocolFor RNAseq analysis, RNA was isolated from organoids as described above. Purified Total RNA was verified by Thermo Scientific NanoDrop one. All RNA samples submitted for bulk RNA-seq were further evaluated for their RIN number (>9) prior to library preparation by the Duke Center for Genomic and Computational Biology (GCB) using the TruSeq stranded total RNA prep kit (Illumina). Sequencing was performed on a NovaSeq 6000 or NextSeq500 by using 150 bp paired-end sequencing. The reads were aligned to the human reference genome (GRCh38) using QIAGEN CLC Genomics (v20). DESeq2 (Love et al., 2014) was used to normalized count data and to perform differential gene expression analysis using a significance cutoff of 0.01 and a fold change cutoff of log2 ±2. Heatmaps and hierarchical clustering were performed in R using pheatmap package in R and were based on RPKM (reads per kilobase million). Mapping to HCMV genomes were performed in CLC Genomics (v20) using either AD169r (accession FJ527563) or TB40E (accession MW439039) reference sequences. Files associated with RNA-seq studies have been deposited into Sequence Read Archive under accession number SUB11885513. Previous data sets used in this study include those from PHT cells (accession PRJNA305417 and PRJNA388219) (Bayer et al., 2016; Corry et al., 2017; Van Twisk et al., 2017), first trimester placental tissue (PRJEB38810), and full-term tissue (PRJNA721668) (Lu-Culligan et al., 2021). Principal component analyses were performed using pcaExplorer in R (Marini and Binder, 2019) or GraphPad Prism. Volcano plots were generated using the EnhancedVolcano package in R (Blighe et al., 2021) or in Graphpad Prism version 9.
Luminex assays
Request a detailed protocolAll sample processing was performed in duplicate and each experiment was performed with at least three biological replicates. Luminex assays were performed using the following kits according to the manufacturer’s instructions: Bio-Plex Pro Human Inflammation Panel 1, 37-Plex kit (171AL001M; Bio-Rad) Bio-Plex Human Chemokine Panel, 40-plex (171AK99MR2; Bio-Rad), Bio-Plex Pro Human Cytokine Screening Panel, 48-Plex kit (12007283; Bio-Rad), hCG Human ProcartaPlex Simplex Kit (Thermo Fisher Scientific, EPX010-12388-901), and CA125 (Mucin-16) Human ProcartaPlex Simplex Kit (EPX010-12437-901). Plates were read on MAGPIX (EMD Millipore) or Bio-Plex 200 (Bio-Rad) Luminex machines and analyzed using xPONENT (EMD Millipore) or Bio-Plex (Biorad) software.
IFN-λ activity reporter assay
Request a detailed protocolFor IFN-λ activity detection from CM, HEK-Blue IFN-λ reporter cells were used according to the manufacture’s protocol. Briefly, IFN-λ reporter cells were plated into a 96-well plate, then CM was added and incubated at 37oC for 24 hr. Supernatants (20 µL) were collected and then transferred into a 96-well plate and activity measured using QUANTI-Blue Solution (InvivoGen, rep-qbs) after incubating at 37oC for 2 hr using a spectrophotometer at 655 nm. Each CM preparation was run in triplicate.
Poly (I:C) and ruxolitinib treatment
Request a detailed protocolFor Poly (I:C) treatment, TOs or DOs were incubated with 10 µg/mL Poly (I:C) (Invivogen, tlrl-pic) diluted in organoid complete growth media for ~24 hr, then supernatant was collected for Luminex-based multianalyte profiling or RNA isolated as described for qRT-PCR or RNASeq. For ruxolitinib (Invivogen, tlrl-rux) treatment, TOs or DOs plated as previously described for HCMV infections were pre-treated with 20 µM ruxolitinib in appropriate complete growth media for 1 hr at 37oC, then were infected with GFP-tagged AD169r in the presence of ruxolitinib for 48 hr.
Statistics and reproducibility
Request a detailed protocolAll experiments reported in this study have been reproduced using independent samples (tissues and organoids) from multiple donors. All statistical analyses were performed using Prism software (GraphPad Software). Data are presented as mean ± SD, unless otherwise stated. Statistical significance was determined as described in the figure legends. Parametric tests were applied when data were distributed normally based on D’Agostino-Pearson analyses; otherwise, nonparametric tests were applied. P values <0.05 were considered statistically significant, with specific p values noted in the figure legends.
Appendix 1
Data availability
Sequence data have been deposited into Sequence Read Archives, PRJNA869668, PRJNA869760, and PRJNA875665.
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NCBI BioProjectID PRJNA869668. Homo sapiens Raw sequence reads.
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NCBI BioProjectID PRJNA869760. BioProject.
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NCBI BioProjectID PRJNA875665. Bulk RNASeq from decidua and trophoblast organoids from human full-term placental tissue.
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NCBI BioProjectID PRJNA305417. Transcriptome comparisons between two-dimensional and three-dimensional cultured JEG-3 trophoblasts, primary human trophoblast cells, and human brain microvascular endothelial cells (HBMEC).
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NCBI GenBankID FJ527563. Human herpesvirus 5 strain AD169, complete genome.
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NCBI GenBankID MW439039. Human betaherpesvirus 5 strain TB40/EE, complete genome.
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NCBI BioProjectID PRJNA388219. Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.
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NCBI BioProjectID PRJEB38810. RNA-seq of human first and second trimester placenta.
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NCBI BioProjectID PRJNA721668. SARS-CoV-2 infection in pregnancy is associated with robust inflammatory response at the maternal-fetal interface (human).
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Article and author information
Author details
Funding
National Institute of Allergy and Infectious Diseases (NIHAI145828)
- Carolyn B Coyne
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
The authors thank Jon Boyle (University of Pittsburgh) for careful review of the manuscript and the Duke University School of Medicine for the use of the Sequencing and Genomic Technologies Shared Resource, which provided RNASeq services. This project was supported by NIHAI145828 (CBC).
Copyright
© 2022, Yang et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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