Salvador Dura-Bernal, Benjamin A Suter ... William W Lytton
The NetPyNE software tool provides a framework to efficiently develop, simulate, optimize and analyze experimentally grounded neural models spanning the molecular, cellular and circuit scales.
An effective and reversible mouse glaucoma model that replicates the secondary glaucoma in human patients caused by silicone oil after retina surgeries presents significant neurodegeneration, and is suitable for neuroprotectants selection.
Frederick Verbruggen, Adam R Aron ... C Nico Boehler
Clear guidelines and open-source software resources are provided for the stop-signal task, in order to streamline the use of this important tool, and to thereby increase its validity and reliability.
New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.
An unbiased RNAseq based strategy to identify targetable pathways and a streamlined lentiviral system provide a state of the art advance on direct fibroblast to neuron conversion.
Maria Pernemalm, AnnSofi Sandberg ... Janne Lehtiö
Comprehensive mass spectrometry analysis of human plasma proteome reveals tissue leakage proteins, describes variability between individual plasma proteomes and demonstrates protein transfer across the placenta during pregnancy.
The design and validation of an oligo-based DNA FISH library that targets the entire Caenorhabditis elegans genome at chromosome, three megabase, and 500 kb scales.
By using a specialized camera that corrects for eye blur, millions of single-blood-cells are imaged, and their speed measured, as they travel through the largest-to-smallest vessels of the retina.
Loïc Binan, François Bélanger ... Santiago Costantino
Single cells from a large heterogeneous population can be identified, isolated and clonally expanded using commonly available microscopy equipment and simple reagents, based solely on visual characteristics.
A transcriptome dataset of nearly 200 genetically identified mouse neuronal cell types revealed that short low-noise homeobox transcription factors and long neuronal effector genes best distinguish neuronal cell types.
