1. Cell Biology
  2. Immunology and Inflammation

Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes

  1. Yiran Zang
  2. Ming Wan
  3. Min Liu
  4. Hongmei Ke
  5. Shuangchun Ma
  6. Lu-Ping Liu
  7. Jian-Quan Ni
  8. José Carlos Pastor-Pareja  Is a corresponding author
  1. Tsinghua University, China
https://doi.org/10.7554/eLife.07187
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Cite this article
  1. Yiran Zang
  2. Ming Wan
  3. Min Liu
  4. Hongmei Ke
  5. Shuangchun Ma
  6. Lu-Ping Liu
  7. Jian-Quan Ni
  8. José Carlos Pastor-Pareja
(2015)
Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes
eLife 4:e07187.
https://doi.org/10.7554/eLife.07187
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Abstract

Many chronic diseases are associated with fibrotic deposition of Collagen and other matrix proteins. Little is known about the factors that determine preferential onset of fibrosis in particular tissues. Here we show that plasma membrane (PM) overgrowth causes pericellular Collagen accumulation in Drosophila adipocytes. We found that loss of Dynamin and other endocytic components causes pericellular trapping of outgoing Collagen IV due to dramatic cortex expansion when endocytic removal of PM is prevented. Deposits also form in the absence of negative Toll immune regulator Cactus, excess PM being caused in this case by increased secretion. Finally, we show that trimeric Collagen accumulation, downstream of Toll or endocytic defects, activates a tissue damage response. Our work indicates that traffic imbalances and PM topology may contribute to fibrosis. It also places fibrotic deposits both downstream and upstream of immune signaling, consistent with the chronic character of fibrotic diseases.

https://doi.org/10.7554/eLife.07187.001

eLife digest

In animals, so-called ‘basement membranes’ surround organs and help to both anchor certain tissue types together and control which molecules move between them. The basement membrane is made up of various proteins, and a large protein called Collagen IV is the most abundant component.

Collagen IV is made inside cells and packaged into bubble-like compartments called vesicles. These vesicles then merge with the cell membrane, which releases the collagen into the space outside the cell. Sometimes, after it has been released from the cell, Collagen IV forms harmful aggregates that the body finds difficult to break down. This condition is known as fibrosis, and can severely damage organs and tissues.

Zang, Wan et al. have now studied how fat cells—also known as adipocytes—in the fruit fly Drosophila melanogaster release Collagen IV. This fly is widely used to study collagen production because it is relatively easy to perform genetic investigations on it, and it releases collagen from its cells in the same way as many other species. Unexpectedly, it was observed that proteins that control a process known as endocytosis—which takes substances into the cell—are also involved in releasing Collagen IV from the cell. Zang, Wan et al. found that this is because endocytosis removes part of the cell membrane: if endocytosis is blocked, then the excess cell membrane traps Collagen IV molecules after they have been released, causing aggregates like those seen during fibrosis. However, artificially decreasing the amount of cell membrane restored normal collagen release.

Zang, Wan et al. next found that a pathway called Toll, which is important for protecting flies against infections, can also affect collagen release. When a protein that inactivates the Toll pathway is absent, too much cell membrane grows and Collagen IV forms aggregates as well. In both cases, Toll activation or lack of endocytosis, the aggregates trigger a reaction that damages the adipocytes. Understanding this reaction in more detail could help to develop treatments for conditions that produce fibrosis.

https://doi.org/10.7554/eLife.07187.002

Introduction

Basement membranes are polymers of extracellular matrix (ECM) proteins that underlie epithelia and surround organs in all animals (Yurchenco, 2011; Kelley et al., 2014). Their main constituent is Collagen IV, a helical trimer consisting of three α chains, capable of forming polymeric networks that interact with other ECM proteins. The fruitfly Drosophila melanogaster has emerged in recent years as an excellent model to study production of Collagen and other ECM proteins thanks to evolutionary conservation, powerful genetic tools and little genetic redundancy (Denef et al., 2008; Martinek et al., 2008; Bunt et al., 2010; Haigo and Bilder, 2011; Drechsler et al., 2013; Lerner et al., 2013; Na et al., 2013; Devergne et al., 2014; McCall et al., 2014; Xiao et al., 2014; Zhang et al., 2014). Two Collagen IV chains exist in Drosophila, encoded by Collagen at 25C (Cg25C, α1 chain) and viking (vkg, α2 chain). Apart from Collagen IV, BMs include Laminin, Nidogen and Perlecan, which are conserved from flies to humans as well (Hynes, 2012). In the larva, the main source of Collagen IV is the adipocytes of the fat body (Pastor-Pareja and Xu, 2011), known for their role in lipid storage and metabolic regulation, but also as an active secretory tissue which produces serum proteins and clotting factors normally present in the hemolymph (insect blood). The fat body is also a key effector of innate immunity, known to produce and secrete to the hemolymph large amounts of antimicrobial peptides in response to infections (Lemaitre and Hoffmann, 2007).

Secreted proteins like Collagen reach the extracellular space through a controlled series of membrane traffic events ensuring fusion of secretory vesicles with the plasma membrane (PM). Besides its role in cargo transport, membrane trafficking is a well-recognized driver of changes in cell shape and PM amount during morphogenesis (Lecuit and Pilot, 2003). Examples of morphogenetic traffic in Drosophila include contraction of the amnioserosa during dorsal closure (Mateus et al., 2011) and widening of the lumen of tracheae (Tsarouhas et al., 2007). The best studied example of traffic-driven morphogenesis is perhaps blastoderm cellularization in the early Drosophila embryo. During blastoderm cellularization, fast directed PM growth results from membrane contributions from the secretory pathway (Lecuit and Wieschaus, 2000), endocytic membrane recycling (Pelissier et al., 2003; Sokac and Wieschaus, 2008; Fabrowski et al., 2013) and microvillar PM elaborations (Figard et al., 2013). While these examples highlight the potential of membrane traffic to elicit drastic changes in cell shape in the context of morphogenetic events, a role of in maintaining stable cortical morphology has not been addressed in detail and little is known on how cells normally regulate PM amount. Furthermore, the consequences for cell physiology of changes in this fundamental property are also unknown.

Handling of Collagen entails several challenges to secreting cells. Because of its large size, secretory transport of Collagen molecules requires carriers larger than regular COPII vesicles (Saito et al., 2009). Also, Collagen molecules undergo postranslational modification along the secretory pathway by numerous Collagen-modifying enzymes such as glycosidases, and lysyl- and prolyl-hydroxylases, required for trimer formation (Myllyharju and Kivirikko, 2004). Prolyl-hydroxylation in particular is essential for trimer formation, mediated in Drosophila by the prolyl-4-hydroxylase PH4αEFB (Pastor-Pareja and Xu, 2011). Unlike fibrilar Collagen I, which flies lack, Collagen IV is secreted in functional form and does not require extracellular cleavage of the N- and C-terminal propeptides (Khoshnoodi et al., 2008). Therefore, and given its ability to form supramolecular assemblies, it is not known how Collagen IV avoids aggregation inside secreting cells or at their PM. Finally, because Collagen is resistant to most proteases, disposing of aggregates when they occur is highly problematic. Such is the case of fibrotic diseases, characterized by aberrant and excessive deposition of ECM due to persistent immune stimulation following diverse types of injuries (Wynn and Ramalingam, 2012). In a variety of tissues such as the liver, skin, kidney or fat tissue, fibrosis disrupts tissue organization and increases matrix stiffness, affecting normal cell and tissue physiology (Hoffman et al., 2011; Friedman et al., 2013).

In this work, we conducted a screening for genes involved in Collagen IV secretion by the fat body adipocytes in Drosophila. We found that loss of shibire and other endocytic genes leads to excess PM growth, which traps Collagen IV in the pericellular space. Pericellular Collagen IV trapping also results upon loss of loss of negative immune regulator cactus, which causes PM overgrowth through a Toll-dependent secretory burst. The accumulation of Collagen IV in the secreting adipocytes has two main consequences: Collagen IV deficit in the BMs of destination tissues and an immune response against adipocytes due to the abnormal ECM accumulation.

Results

Endocytic defects cause Collagen IV accumulation in Drosophila adipocytes

To gain new insights into Collagen biogenesis, we conducted a screening for genes affecting production of Collagen IV by fat body adipocytes, its main source in the Drosophila larva (Figure 1A). We used BM-40-SPARC-GAL4 (Venken et al., 2011) to drive expression in adipocytes of the RNAi transgenes in the TRiP collection (8459 lines targeting 6200 genes) (Ni et al., 2008, 2011) and analyzed the localization of Vkg-GFP, a functional GFP-trap fusion to the Collagen IV chain Vkg (Morin et al., 2001). While a majority of hits produced intracellular Collagen IV accumulation (full results to be published later), a distinct phenotypical category consisted of 60 genes causing accumulation at or near the PM (Supplementary file 1). Among the strongest hits in this category were two different RNAi transgenes targeting shibire (shi), encoding fly Dynamin, a GTPase involved in excision of endocytic vesicles (Ferguson and De Camilli, 2012). shibire knock-down (shii), as well as expression of dominant negative Dynamin (ShiK44A) (Moline et al., 1999), caused Vkg accumulation in adipocytes (Figure 1B). In validation of the phenotype, antibody staining confirmed reduced Dynamin expression in shii cells, whereas the staining increased after Shi.K44A overexpression (Figure 1C), attesting to the sensitivity of the antibody. Since Collagen IV is a heterotrimer combining the α2 chain Vkg with Cg25C α1 chains, we performed a staining with an anti-Cg25C antibody we generated for this study (see Figure 1—figure supplement 1). This staining revealed that Cg25C, same as Vkg, accumulates in shii adipocytes (Figure 1D), thus confirming Collagen IV accumulation in these cells. The accumulation of Collagen IV occurs at the cell periphery, under a basement membrane surrounding the tissue in the wild type and which still forms in shii adipocytes (Figure 1D; see also Figure 3B later). Further validating the requirement of shibire in normal Collagen IV distribution, shi1 and shi2 thermosensitive mutations (Kim and Wu, 1990) also caused Vkg accumulation in adipocytes when larvae grew at restrictive temperature (Figure 1—figure supplement 1).

Figure 1 with 2 supplements see all
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Endocytic defects cause Collagen accumulation in Drosophila adipocytes.

(A) Schematic depiction of Collagen IV production, secretion and incorporation into basement membranes. (B) shibire knock-down (BM-40-SPARC>shii) and dominant negative shibireK44A (BM-40-SPARC>shiDN) cause Vkg-GFP accumulation in third instar larva adipocytes (marked with RFP). (C) Confocal images of third instar larva adipocytes stained with anti-Dynamin antibody. Staining is absent upon shi knock-down and increased by shiK44A expression. Nuclei stained with DAPI. (D) Localization of Vkg-GFP and Cg25C (anti-Cg25C staining) in wild type and BM-40-SPARC>shii adipocytes. Collagen IV accumulates in the periphery of shii adipocytes. (E) Vkg accumulation in BM-40-SPARC>Rab5i and >Rab5DN adipocytes. (F) Vkg accumulation in BM-40-SPARC>Chci and >ChcDN adipocytes. (G) Presence of Vkg-GFP is reduced in discs from BM-40-SPARC>shii, >shiDN, >Rab5i and >Rab5DN larvae. Vkg-GFP decrease quantified in graph. n ≥ 5 for each genotype. Differences with wild type are in all cases significative (Mann–Whitney tests, **p < 0.01). (H) Elongation of the ventral nerve cord (VNC) in BM-40-SPARC>shiDN and >Rab5DN.

https://doi.org/10.7554/eLife.07187.003

In addition to Dynamin, a number of hits showing a similar peripheral Collagen IV accumulation phenotype in the screening turned out to encode components of the endocytic machinery as well, such as Rab5 (Figure 1E), Clathrin heavy chain (Figure 1F), the Rab5-GAP RN-Tre, the AP-2 components AP-2α and AP-2μ and Hrs (Figure 1—figure supplement 1), suggesting that defective endocytosis was indeed the cause for the phenotype. Concurrent with the accumulation of Collagen IV in the adipocytes producing it, we found that the amount of Collagen IV present in destination BMs (Figure 1G) and in the hemolymph (Figure 1—figure supplement 1) was reduced in these conditions. In agreement with this, we observed elongation of the ventral nerve cord (Figure 1H), a deformation associated with Collagen IV reduction (Pastor-Pareja and Xu, 2011). We therefore conclude from these data that loss of shibire and other endocytic genes causes accumulation of Collagen IV in the adipocytes that normally secrete it and set out next to ascertain the mechanism by which this accumulation occurs.

Collagen accumulation in endocytosis-defective cells is pericellular and autonomous

Accumulations of Collagen IV in shii adipocytes were found in close proximity to the PM, as revealed by the membrane marker myr-mRFP (myristoylation domain of Src fused to mRFP; Figure 2A) and by phalloidin staining of F-actin, normally enriched in the cell cortex (Figure 2B). To determine whether Collagen IV accumulations were intracellular or extracellular we stained shii adipocytes with the lipophilic, cell-impermeable dye FM4-64 (Bolte et al., 2004) to label membrane directly in contact with the extracellular space (PM). Short incubation with FM4-64 showed that the accumulations were surrounded by PM (Figure 2C) and, thus, likely extracellular. Labeling of membrane around the accumulations was equally observed when shii adipocytes were fixed and stained with fluorescently-labeled fixable dextrans of molecular weights 70,000 (Figure 2D) and 3000 (Figure 2—figure supplement 1). To confirm that the Collagen IV accumulations in shii adipocytes were extracellular, we performed antibody stainings without permeabilizing the cells (no detergent in washing or blocking solutions). As a control, we stained Tango1i adipocytes, known to retain Collagen IV intracellularly (Pastor-Pareja and Xu, 2011), and found that intracellularly retained Collagen IV could not be stained without permeabilization (Figure 2E). In contrast to this lack of staining, accumulations of Collagen IV in shii adipocytes were still labeled (Figure 2E), indicating that they were indeed extracellular. Altogether, these data show that Collagen IV accumulations in shii adipocytes are pericellular accumulations located outside of the PM (see also electron micrographs in Figure 2—figure supplement 1).

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Collagen accumulation in endocytosis-defective cells is pericellular and autonomous.

(A) Vkg-GFP accumulation in a shii adipocyte (BM-40-SPARC>shii) expressing membrane marker myr-RFP. (B) shii adipocyte stained with phalloidin (F-actin). (C) shii adipocyte stained with cell-impermeable membrane dye FM4-64, labelling plasma membrane (PM) around accumulations. (D) shii adipocyte stained with fixable Texas-Red-coupled Dextran (70,000 MW), labelling PM around accumulations. (E) Antibody stainings of wild type and shii adipocytes performed without permeabilization (no detergent) in order to detect extracellular Collagen IV. In contrast to the accumulations in shii adipocytes, intracellular accumulations of Collagen IV in Tango1i adipocytes cannot be stained in the absence of permeabilization and are shown as a control. (F) Mosaic fat body (act-GAL4>shii flip-out clones, marked with RFP) showing Vkg-GFP accumulation in shii cells. Accumulation is suppressed by a GFP-targeting dsRNA (iGFPi). (G) Pericellular accumulation in mosaic shii fat body expressing Cg25C-RFP. Clones marked with GFP. (H) Localization of the endocytic marker Transferrin Receptor (Cg>TfR-GFP) in wild type adipocytes. (I) In shii adipocytes, TfR concentrates in PM pockets containing Collagen IV (anti-Cg25C). (J) In Rab11i adipocytes, TfR localizes to intracellular vesicles that completely fill the cytoplasm. No TfR is detected at the PM.

https://doi.org/10.7554/eLife.07187.006

In light of the accumulation of Collagen IV, we first hypothesized that Collagen was internalized from the hemolymph (blood) by wild type adipocytes, which could explain pericellular accumulation when endocytosis was prevented. Contradicting this hypothesis, however, we found in mosaic experiments that accumulation of Vkg-GFP was suppressed when Vkg-GFP expression was knocked-down in the same cell (Figure 2F). This result demonstrates that Collagen IV accumulated in a Dynamin-deficient adipocyte originates autonomously in that same cell. Supporting the autonomous origin of accumulated Collagen IV, mosaic expression of Cg25C-RFP (see Figure 2—figure supplement 1) in shii adipocytes caused autonomous Collagen IV accumulation as well (Figure 2G). These experiments indicate that Collagen IV pericellularly accumulating in a shii adipocyte is not Collagen that failed to be endocytosed. Contrary to that, our data show that pericellularly accumulated Collagen IV, instead of ingressing into the cell, is outgoing Collagen secreted by that same cell and becoming extracellularly trapped in the cell cortex.

Pericellular Collagen trapping in endocytosis-defective cells is due to PM overgrowth

In order to ascertain how endocytic defects cause pericellular trapping of outgoing Collagen, we proceeded to characterize the locus of its accumulation. The endocytic marker TfR (Transferrin Receptor, hTfR-GFP [Henthorn et al., 2011]) localized uniformly in the adipocyte PM and some intracellular vesicles (Figure 2H). Confirming that TfR is internalized and recycled back to the PM, knock-down of Rab11, required for recycling endosome trafficking (Maxfield and McGraw, 2004), caused TfR vesicles to fill the cytoplasm while no TfR was detected at the PM (Figure 2J). In Dynamin-deficient cells, in contrast, TfR concentrated heavily in deep PM pockets containing Collagen IV at their center (Figure 2I), suggesting that Collagen is pericellularly accumulated in PM pockets formed as a consequence of failed endocytosis.

To characterize in further detail the topology of the PM at the site of Collagen accumulation, we analyzed confocal and electron micrographs. Wild type adipocyte PM, which is flat during the first and second larval instars, becomes somewhat convoluted in the third instar (Figure 3—figure supplement 1), displaying multiple invaginations which often encircle surface lipid droplets (Diaconeasa et al., 2013). Compared to this, examination of endocytosis-deficient adipocytes revealed a striking increase in PM amount with respect to the wild type (Figure 3A,B). Quantification in both confocal and electron micrographs showed an increase in the depth of PM ingression into the cytoplasm and higher PM sinuosity in endocytosis-defective cells (Figure 3C; Figure 3—figure supplement 2). The opposite phenotype, PM flattening, along with a vesicle-filled cytoplasm, resulted from Rab11 knock down. These results indicate that both endocytosis and membrane recycling are critical to maintain normal PM amount and cortical morphology, suggesting that Collagen IV is trapped in an abnormally expanded cell cortex when endocytic removal of membrane from the PM is prevented.

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Pericellular Collagen trapping is due to PM overgrowth.

(A) Confocal sections of adipocyte PM (myr-RFP marker). PM expansion is observed in BM-40-SPARC>shii, >Rab5i and >Chci adipocytes, whereas PM flattening occurs in >Rab11i adipocytes along with accumulation of intracellular vesicles. (B) Electron micrographs of adipocyte PM from control, BM-40-SPARC>shii, >Rab5i, >Chci and >Rab11i larvae. Internal cell volume indicated through yellow transparency. Asterisks mark pericellular deposits. (C) Quantification of PM depth and sinuosity (see Figure 3—figure supplement 2). Depth measurements obtained from confocal (n = 12) and electron (n = 10) micrographs. PM sinuosity is the ratio between the length of PM between two points on that membrane and the linear distance separating them (n ≥ 15). Differences with controls were significative as indicated (Mann–Whitney tests, **p < 0.01, ***p < 0.001). (D) Adipocytes from wild type larvae grown on lipid-depleted food, BM-40-SPARC>shii larvae and BM-40-SPARC>shii larvae grown on lipid-depleted food. Vkg-GFP accumulation and PM excess are both suppressed by lipid-depletion.

https://doi.org/10.7554/eLife.07187.008

To confirm that an excessive amount of PM can act as a barrier to Collagen release after secretion, we aimed at artificially decreasing PM amount in adipocytes. To that end, we cultured flies in medium depleted of lipids through chloroform extraction (Palm et al., 2012). In larvae thus grown, we found that the amount of adipocyte PM was greatly reduced (Figure 3C,D; Figure 3—figure supplement 1). Furthermore, lipid-depleted food suppressed both PM excess and pericellular Collagen accumulation in Dynamin-deficient adipocytes (Figure 3C,D), indicating that PM overgrowth was indeed responsible for pericellular Collagen trapping. These data, in all, show that PM excess resulting from lack of endocytic membrane removal leads to a hyperconvoluted PM, which in turn causes pericellular trapping of Collagen IV.

Pericellular deposits in adipocytes are fibrotic

We asked next whether other proteins besides Collagen IV were pericellularly trapped due to PM overgrowth. Apart from Collagen IV, the main components of basement membranes are Perlecan, Laminin and Nidogen (Yurchenco, 2011). Whereas evidence exists of significant Laminin and Nidogen production outside the fat body (Urbano et al., 2009; Zhu et al., 2012), production of Perlecan has not been studied. Through iYFPi (in vivo YFP interference, Figure 4A), we knocked down expression of Perlecan-YFP (trolCPTI-002049 [Rees et al., 2011]), a YFP-trap insertion predicted to label all Perlecan isoforms and found that Perlecan present in imaginal discs originated entirely in the fat body (Figure 4B), same as Collagen IV. Also similar to Collagen IV, Trol-YFP was pericellularly accumulated in endocytosis-defective adipocytes (Figure 5A,B). This accumulation of Trol (terribly reduced optic lobes) depended on Collagen IV, as it was suppressed by Collagen IV knock-down (Figure 5C). Through antibody staining, we confirmed Perlecan accumulation (Figure 5—figure supplement 1) and additionally observed accumulation of Nidogen, again in a Collagen-dependent manner (Figure 5D), but not Laminin (anti-LanB1 staining, not shown).

Figure 4
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Perlecan, like Collagen IV, originates in the fat body.

(A) Schematic representation of the in vivo YFP interference strategy (iYFPi) to knock-down expression of YFP-trapped Perlecan (Trol-YFP) and ascertain its tissue of origin. Expression of a short hairpin RNA targets the YFP sequence in the YFP-trapped mRNA for degradation through RNAi. (B) Localization of Perlecan (Trol-YFP trap) in wing discs from trolCPTI-002049/Y flies. iYFPi in the fat body (BM-40-SPARC>iYFPi) eliminates expression of Trol-YFP in the wing disc and produces tissue hyperconstriction, a previously described trol loss-of-function phenotype (Pastor-Pareja and Xu, 2011). Phalloidin staining of F-actin in red to reveal disc deformation.

https://doi.org/10.7554/eLife.07187.011
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Pericellular deposits in adipocytes are fibrotic.

(A) Localization in wild type and BM-40-SPARC>shii adipocytes of Trol-YFP (trolCPTI-002049; see Figure 4). Perlecan accumulates in >shii adipocytes. (B) Perlecan accumulation in BM-40-SPARC>shiDN and >Rab5i adipocytes. (C) Perlecan accumulation in r4>shii adipocytes is suppressed by Collagen IV knock down. (D) Pericellular Nidogen accumulation (anti-Ndg staining) in r4>shii adipocytes is suppressed by Collagen IV knock down. (E) Pericellular Vkg accumulation in BM-40-SPARC>shii adipocytes is suppressed by knocking down prolyl-hydroxylase PH4αEFB. (F) BM-40-SPARC>shii adipocytes do not accumulate secretion marker secr-GFP. Intracellular secr-GFP retention in BM-40-SPARC>Rab1i is shown as a control.

https://doi.org/10.7554/eLife.07187.012

The fact that the aggregates contained basement membrane components Collagen IV, Perlecan and Nidogen suggested that they were in essence fibrotic ECM deposits. Indeed, Vkg accumulation was suppressed by knock down of the Prolyl-4-hydroxylase PH4αEFB (Figure 5E), in the absence of which Collagen IV is still secreted to the blood as non-functional monomeric chains (Pastor-Pareja and Xu, 2011). Additionally supporting the fibrotic nature of the deposits, the secretion marker secr-GFP (GFP coupled to a signal peptide [Pfeiffer et al., 2002]) did not accumulate in the expanded cortex of shii adipocytes (Figure 5F). We also examined two other non-ECM proteins secreted by the fat body, 26-29-protease and Ferritin 1HCH, and none of them accumulated pericellularly upon shi knock down (Figure 5—figure supplement 1). These results demonstrate that not all proteins secreted by the fat body are pericellularly trapped as a result of PM overgrowth. On the contrary, our data support the notion that the aggregates consist of ECM proteins like trimeric Collagen IV, Perlecan and Nidogen, and thus are fibrotic deposits. Furthermore, our results suggest that Collagen IV is the main protein in this deposits, as accumulation of Perlecan and Nidogen depended on the presence of Collagen IV.

Fibrotic deposits and PM overgrowth upon Toll activation

Having established that extracellular deposits caused by PM overgrowth were not general protein aggregates but rather fibrotic ECM aggregates, we decided to investigate their wider biological effects. To do that, we first turned our attention to cactus, another PM accumulation hit in our screening (Figure 6A). cactus (cact) encodes a negative regulator of the Toll signaling pathway, a key mediator of innate immunity (Roth et al., 1991). We confirmed activation of Toll signaling in cacti adipocytes (Dorsal nuclear accumulation) and further validated the phenotype by observing pericellular Collagen accumulation in cact4 mutants (Roth et al., 1991) (Figure 6—figure supplement 1). Expression of the constitutively active mutant receptor Toll10B also produced pericellular Collagen accumulation (Figure 6B), as did infection with Toll-activating Gram+ bacteria Micrococcus luteus (Figure 6C; Figure 6—figure supplement 1). Examination of cacti and Toll10B adipocytes in confocal and electron micrographs revealed PM overgrowth (Figure 6D,E), similar to shii adipocytes. Also similar to shii adipocytes, PM overgrowth upon Toll activation was suppressed by lipid-depleted food (graph in Figure 6E). Moreover, pericellular Collagen IV deposits were extracellular (antibody staining without permeabilization, Figure 6—figure supplement 1), disappeared upon PH4αEFB knock-down (Figure 6F) and did not contain secr-GFP (Figure 6G), but contained Perlecan (Figure 6H), indicating accumulation of fibrotic material, same as in endocytosis-defective cells.

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Fibrotic deposits and PM overgrowth upon Toll activation.

(A) Pericellular Vkg deposits (Vkg-GFP) and PM overgrowth in BM-40-SPARC>cacti adipocytes. (B) Vkg deposits and VNC elongation in BM-40-SPARC>Tl10B larvae. (C) Vkg deposits and PM overgrowth in adipocytes 1 day after infection with Micrococcus luteus. (D) Electron micrographs of BM-40-SPARC>cacti and >Tl10B adipocytes. The arrow marks connection of the deposits to the extracellular space. (E) Measurements of PM depth and sinuosity in adipocytes of indicated genotypes. Depth measurements obtained from confocal (n ≥ 7) and electron micrographs (n ≥ 10). Sinuosity measured in electron micrographs (n ≥ 15). Differences with wild type or appropriate control as indicated were significative in all cases (Mann–Whitney tests, **p < 0.01, **p < 0.001). (F) Pericellular Vkg accumulation in BM-40-SPARC>cacti and >Tl10B adipocytes is suppressed by knocking down prolyl-hydroxylase PH4αEFB. (G) Secretion marker secr-GFP does not accumulate in BM-40-SPARC>cacti or >Tl10B adipocytes. (H) Pericellular Perlecan deposits (Trol-YFP) in BM-40-SPARC>cacti and >Tl10B adipocytes. (I) Induction of antimicrobial peptide Drosomycin (Drs-GFP) fills adipocyte cytoplasm in BM-40-SPARC>cacti, >Tl10B and Micrococcus luteus-infected larvae. (J) Rab1 knock-down causes intracellular Drosomycin retention and suppresses PM overgrowth in BM-40-SPARC>Tl10B adipocytes.

https://doi.org/10.7554/eLife.07187.014

Despite these similarities with endocytosis-defective cells, we could not find evidence of endocytosis/recycling defects when we examined TfR-GFP localization in cacti and Toll10B adipocytes (Figure 6—figure supplement 1), suggesting that PM overgrowth upon Toll activation did not stem from reduced endocytosis. Toll activation, on the other hand, is known to potently induce production of secreted antimicrobial peptides like Drosomycin, Defensin and Metchnikowin (Lemaitre et al., 1996), which led as to consider increased secretion of these Toll targets as an alternative explanation to PM overgrowth upon Toll activation. Consistent with this, knock-down of Dif, a transcription factor involved in expression of Toll target genes in the adult (Rutschmann et al., 2000) and larval fat body (see Figure 6—figure supplement 1), suppressed PM overgrowth (graph in Figure 6E) and pericellular Vkg-GFP trapping (not shown), suggesting that the transcriptional response to Toll activation contributed to Collagen IV accumulation in cacti and Toll10B adipocytes. Also consistent with a burst in secretory activity causing the PM overgrowth, the cytoplasm of cacti, Toll10B and M. luteus-infected adipocytes was filled with Drosomycin vesicles or granules (Figure 6I; Figure 6—figure supplement 1). Furthermore, knock-down of Rab1, required for ER-to-Golgi transport in the secretory pathway, caused not just abundant intracellular Drs-GFP retention in Toll10B adipocytes, but also suppression of PM excess (Figure 6J), implicating membrane input from the secretory pathway in PM overgrowth. In all, our analysis indicates that PM overgrowth and fibrotic deposits upon Toll activation result from increased secretory activity.

Immune response to fibrotic deposits

Our experiments indicated that fibrotic aggregates could form downstream of Toll immune signaling. We therefore decided to characterize further the immune effects of these deposits. In our screening, 32 out of 70 hits producing pericellular Collagen IV accumulation showed clear signs of a fat body melanization response, including cact, shi, Rab5 and Chc (Supplementary file 1). Melanization is an insect immune response characterized by blackening of the affected tissue, usually accompanied by hemocyte (blood cell) recruitment (Minakhina and Steward, 2006). Because BM-40-SPARC-GAL4 and Cg-GAL4, the strong fat body drivers we had used so far, are also expressed in blood cells, we tested weaker fat body drivers ppl-GAL4 and r4-GAL4, inactive in blood cells, and found that melanization still occurred (Figure 7A,B), ruling out that interfering with blood cell function caused the response. Furthermore, confirming the involvement of Collagen deposits in the response, both the number of r4>shii larvae displaying melanization and the extent of it decreased when we additionally knocked down Collagen IV or PH4αEFB (Figure 7C,D). Reduction of Collagen IV and PH4αEFB also reduced melanization of r4>Toll10B larvae (Figure 7D) and completely rescued their pupal lethality. These results show that Collagen deposits either trigger or significantly contribute to fat body melanization in these conditions.

Figure 7
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Immune response to fibrotic deposits.

(A) Melanotic fat body in a ppl>shii fly. (B) Melanized fat body from an r4>shii larva. Hemocytes (blood cells) encapsulate the tissue. (C) Knock-down of Collagen IV reduces fat body melanization in r4>shii larvae. Cultures maintained at 30°C. (D) Percentage of larvae displaying signs of melanization in indicated genotypes. n ≥ 30 per genotype. Differences with r4>shii and >Tl10B controls were significative (χ2 tests, ***p < 0.001). Cultures maintained at 30°C. (E) Induction of c-Jun N-terminal kinase (JNK) downstream puckered (puc-GFP enhancer trap) in BM-40-SPARC>shii, >Tl10B and >cacti adipocytes. (F) Induction of Matrix Metallo-Protease 1 (anti-Mmp1 staining) in BM-40-SPARC>shii, >Tl10B and >cacti adipocytes. (G) Expression of JAK/STAT activity reporter 10XSTAT-GFP in BM-40-SPARC>shii, >Tl10B and >cacti adipocytes. (H) Expression of JAK/STAT-activating ligands in wild type, BM-40-SPARC>shii and >Tl10B adipocytes assessed by real time RT-PCR. Error bars represent 95% confidence intervals. rp49 expression was used for normalization.

https://doi.org/10.7554/eLife.07187.016

Given that Toll activation is long known to cause melanization (Gerttula et al., 1988), we examined Toll activity in shii adipocytes, but found that shibire knock-down did not cause Toll pathway activation, as evidenced by lack of Drs-GFP induction and absence of nuclear Dorsal localization (not shown). In contrast, both shibire knockdown and Toll activation caused activation of the c-Jun N-terminal kinase (JNK) and Janus kinase/Signal Transducer and Activator of Transcription (JAK/STAT) pathways, known to act together in response to tissue damage in other fly tissues (Pastor-Pareja et al., 2008; Buchon et al., 2009). JNK targets puckered (puc) and Matrix metallo-protease 1 (Mmp1) (Uhlirova and Bohmann, 2006) were induced in shii, Toll10B and cacti adipocytes (Figure 7E–G). As for JAK/STAT signaling, we observed induction of the JAK/STAT reporter 10XSTAT-GFP (Ekas et al., 2006) (Figure 7G) and highly upregulated expression of unpaired 3 (upd3), encoding a JAK/STAT-activating cytokine (Figure 7H). From these data, we conclude that fibrotic deposits caused by endocytic defects or Toll provoke tissue damage in adipocytes and stimulate an innate immune response, as evidenced by tissue melanization, blood cell recruitment, activation of JAK/STAT signaling and JNK (Figure 8).

Figure 8 with 1 supplement see all
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PM overgrowth leads to adipocyte fibrosis.

Schematic representation summarizing the genesis of fibrotic deposits caused by PM excess and the ensuing reaction by the immune system. Defective endocytosis or excess secretion induced by Toll activity cause PM overgrowth in fat body adipocytes, which leads to hyperconvoluted PM morphology and pericellular trapping of Collagen IV and other extracellular matrix (ECM) proteins in the cell cortex. Fibrotic Collagen IV deposits trigger an immune response, as evidenced by tissue melanization and activation of the JAK/STAT and JNK pathways.

https://doi.org/10.7554/eLife.07187.017

Discussion

Excess PM as a barrier to Collagen release after secretion

In this study, we investigated the release of Collagen IV by the fat body adipocytes in Drosophila larvae. Through a genetic screening, we discovered that reducing endocytosis caused unexpected Collagen IV accumulation in secreting cells (Figure 1). In investigating the cause for such a paradoxical phenotype, our experiments revealed that Collagen IV was effectively secreted and reached the extracellular space, but it was then trapped in fibrotic deposits at the cell periphery (Figure 2). Further experiments led us to conclude that endocytic membrane removal and recycling are critical to maintain normal PM amount and that excess of PM when endocytic membrane removal was prevented resulted in a hyperconvoluted cell cortex where Collagen IV became entrapped (Figure 3). This hyperconvoluted cell cortex is reminiscent of the basal labyrinth found in highly secretory cells, such as enterocytes and renal tubule epithelium. Fibrotic deposits in the absence of negative Toll regulator Cactus were similarly caused by PM overgrowth as well (Figure 6). In this instance, however, PM overgrowth resulted from increased membrane input from the secretory pathway, consistent with the ability of Toll to induce massive secretion of antimicrobial peptides. Supporting this, knock down of Rab1, required for ER-to-Golgi transport, was able to suppress Toll-induced PM overgrowth. Therefore, both recycling endosome-derived and ER-derived membrane inputs may contribute to total PM amount while constant endocytic membrane removal prevents PM overgrowth.

Sudden surges or shifts in membrane traffic are well known drivers of cell shape changes during development (Lecuit and Pilot, 2003). However, the extent to which maintenance of a stable cell shape is affected by the balance of membrane outputs and inputs to the PM has been less explored. Our experiments show that endocytosis is critical for preserving cortex morphology in Drosophila adipocytes and that preventing endocytosis can cause dramatic PM overgrowth. A factor that might determine high PM turnover in these cells, and thus a tendency to imbalances, is their elevated secretory activity. Apart from Collagen IV, fat body adipocytes secrete most other proteins present in the hemolymph, including the very abundant larval serum proteins (Guedes Sde et al., 2003; Vierstraete et al., 2003), which imposes a constant input of ER membrane to the PM. A need for control of PM amount, however, does not seem exclusive to adipocytes or highly secretory cells based on reported effects in other cell types. For instance, in Garland cells, a filtering cell type with high endocytic activity, shibire loss causes deep PM tubulation (Kosaka and Ikeda, 1983), whereas loss of Rab11 flattens the PM (Satoh et al., 2005). In neurons, accumulation of membrane cisternae in synaptic boutons was observed upon Clathrin and Dynamin photoinactivation (Heerssen et al., 2008; Kasprowicz et al., 2014), similar to the PM excess we found in adipocytes. Finally, Rab5 and other endocytic genes are required to maintain epithelial polarity (Lu and Bilder, 2005; Zeigerer et al., 2012), suggesting that constant endocytosis actively keeps apical and baso-lateral PMs segregated. These examples, together with our findings, point to a requirement for endocytosis and recycling in maintaining stable cell shape wider than currently appreciated.

In addition to the finding that membrane traffic controls adipocyte PM amount, our results have implications for the understanding of fibrotic deposits. We arrived at the study of membrane traffic in adipocytes through a Collagen IV accumulation phenotype. Collagen IV, however is not the only protein in these deposits (Figure 5). Also Perlecan, which we show originates in adipocytes as well (Figure 4), accumulates pericellularly. Except for Nidogen, a third basement membrane component, no other protein we tested accumulated, including Ferritin and Catalase, two of the most abundant proteins in the blood (Handke et al., 2013). Even though a more complete view of the aggregates must await a proteomic analysis, our data suggest that Collagen IV, while not the only protein present in these deposits, it is the most important one, as knock down of Collagen IV suppressed accumulation of Perlecan and Nidogen in shii cells. Because of its abundance and ability to self-interact, Collagen IV seems particularly prone in principle to nucleate aggregates in the conditions of limited diffusion provided by a hyperconvoluted PM. That Collagen IV self–interaction is critical in triggering pericellular aggregation is indeed supported by the fact that knock-down of PH4α-EFB, required for trimer formation, suppresses aggregate formation. In contrast, our data showing that PM surrounding aggregates can be stained equally well with both low (3000) and high (70,000) molecular weight fixable dextrans (Figure 2—figure supplement 1) suggests that the size of the accumulated protein does not play a major role in the formation of the deposits. This is also supported by the finding that Perlecan, a large 400 kDa protein, does not accumulate by itself, but as a result of Collagen IV accumulation (Figure 5).

Besides endocytic genes and cactus, our screening has found additional hits causing Collagen IV accumulation at the PM (Supplementary file 1). Loss of BM-40-SPARC, one of these hits, was previously known to cause Collagen IV deposits on adipocytes (Pastor-Pareja and Xu, 2011). Furthermore, similar to the fibrotic deposits in our study, other basement membrane components accumulate upon loss of BM-40-SPARC (Shahab et al., 2015). However, the PM is not overgrown in BM-40-SPARCi adipocytes (Figure 8—figure supplement 1), suggesting that the way in which aggregates form in BM-40-SPARCi adipocytes is different from the way they arise in endocytosis-defective and Toll-activated adipocytes. We have also tested Toll pathway activation by examining expression of Toll target Drosomycin (Drs-GFP) in BM-40-SPARCi and all other PM accumulation hits, but found no Drosomycin-GFP induction in any of them. This suggests that, cactus aside, none of the hits causes Collagen IV deposits through ectopic Toll activation. Further characterization work, therefore, is needed to ascertain the particular mechanisms by which PM accumulation of Collagen IV occurs upon loss of these genes. Such work might offer additional insights for a better understanding of fibrosis.

Adipocyte fibrosis

Pericellular trapping of Collagen IV, apart from preventing its incorporation to destination tissues, induces a potent response in adipocytes. Aspects of this response include melanization and activation of the JAK/STAT and JNK pathways (Figure 7). Concomitant activation of JNK and JAK/STAT signaling makes this response reminiscent of tissue damage responses in imaginal discs and gut tissues (Pastor-Pareja et al., 2008; Buchon et al., 2009). In particular, predominance of upd3 among induced JAK/STAT cytokines is shared with the intestinal response to tissue damage (Buchon et al., 2009). It has been suggested that basement membrane disruption could be a signal that intestinal cells sense when flies are fed tissue damage-inducing Dextran Sulfate Sodium (Amcheslavsky et al., 2009). Absence of basement membrane, although unable by itself to elicit a response (Pastor-Pareja et al., 2008), has been recently linked to melanization as a permissive factor (Hauling et al., 2014; Kim and Choe, 2014). In the case of adipocyte fibrosis, however, our data indicate that it is the fibrotic deposits that cause the response rather than absence of a basement membrane, since Collagen IV knock down in shii cells suppressed the response instead of enhancing it. Also in Toll10B adipocytes, Collagen IV reduction partially suppressed melanization, showing that, even though Toll targets include melanization enzymes (De Gregorio et al., 2002), Collagen deposits contribute to Toll-induced melanization. An interesting question deserving of further investigation is how the fly immune system can react to these fibrotic deposits. One possibility is that fibrotic deposits are directly detected as altered self by the immune system, for instance through recognition of some specific molecular trait in them. A second possibility is that deposits somehow cause damage in adipocytes and this damage activates the response. In this latter case, the nature of the fibrosis-induced cell damage and the way that damage might be sensed are also worth deeper investigation.

Adipocyte fibrosis in humans and mouse models is correlated with obesity and adipose tissue dysfunction (Sun et al., 2013). Same as human adipocytes, Drosophila larval adipocytes are surrounded by a basement membrane containing Collagen IV (Chun, 2012); also similar, human adipocytes display complex PM topology, with abundant caveolae forming higher-level rosettes (Fan et al., 1983). Our study, importantly, provides a model to study fibrosis in this tissue. Fibrosis, affecting adipocytes or otherwise, is thought to result in all cases from inflammatory stimulation of excess ECM deposition. Our results show that accumulation of trimeric Collagen IV can be triggered by Toll immune activation and that deposits in turn further stimulate other arms of the fly immune system different from the Toll-mediated immune response. This raises the possibility that fibrotic accumulations, once initiated, could be self-sustained, with inflammation and fibrosis feeding each other. Interestingly, the response to fibrosis includes induction of Collagen-degrading enzyme Matrix metalloprotease 1, suggesting that the response is aimed in part at clearing the aggregates. Further research into how innate immunity is activated by these deposits may uncover therapeutically relevant mechanisms by which adipocytes and other tissues react to, and deal with, excessive matrix deposition.

Materials and methods

Drosophila strains and culture

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Standard fly husbandry techniques and genetic methodologies, including balancers and dominant genetic markers, were used to assess segregation of mutations and transgenes in the progeny of crosses, construct intermediate fly lines and obtain flies of the required genotypes for each experiment (Roote and Prokop, 2013). Flies were maintained at 25°C unless otherwise stated. In the initial screening and most experiments afterwards, the GAL4-UAS binary expression system (Brand and Perrimon, 1993) was used to drive expression of UAS transgenes in fat body adipocytes under temporal and spatial control of transgenic GAL4 drivers BM-40-SPARC-GAL4 (Venken et al., 2011) (a gift from Hugo Bellen), Cg-GAL4 (Asha et al., 2003) (BL7011), r4-GAL4 (Lee and Park, 2004) (a gift from Pierre Leopold) and ppl-GAL4 (Colombani et al., 2003) (a gift from Herve Agaisse). In flies bearing both types of transgenes (a GAL4 driver and a UAS responder), expression of the UAS-transgene is induced by the yeast transcription factor GAL4 according to the pattern of expression of GAL4 specific to the driver transgene. The strength of these drivers is BM-40-SPARC-GAL4 > Cg-GAL4 > r4-GAL4 > ppl-GAL4. While BM-40-SPARC-GAL4 and Cg-GAL4 are expressed in blood cells, r4-GAL4 and ppl-GAL4 are not. UAS-Dcr2 was included in the screening strain for the purpose of enhancing RNAi-mediated knock-down by long dsRNA hairpins (Dietzl et al., 2007). Dcr2 expression in adipocytes showed no visible effect by itself on Collagen IV localization (Figure 1—figure supplement 2). For generation of flip-out clones (Ito et al., 1997) (Figure 2F,G), vials containing L2 larvae were heat-shocked at 37°C for 7 min. For lipid depletion experiments, lipids were extracted from medium by mixing ingredients with chloroform for 2 days, allowing chloroform to evaporate for at least two more days before food preparation (Palm et al., 2012). Genotypes of animals in all experiments are detailed in Supplementary file 2. Origin of mutants and transgenes used can be found in Supplementary file 3. The following strains were used:

Canton-S

w1118

y w; vkgG454/CyO

w; vkgG454 UAS-myr-RFP; BM-40-SPARC-GAL4 UAS-Dcr2/SM6a-TM6B

w; vkgG454/CyO; BM-40-SPARC-GAL4 UAS-myr.RFP/TM6B

w; Cg-GAL4 (II)

w; Cg-GAL4 UAS-myr-RFP (II)

w; ppl-GAL4 (II)

w; ppl-GAL4 UAS-myr.RFP vkgG454/CyO

w; ppl-GAL4 UAS-myr.RFP vkgG454/CyO; UAS-Dcr2

w; r4-GAL4 (III)

w; UAS-myr.RFP vkgG454/CyO; r4-GAL4

y sc v; UAS-shi.RNAiTRiP.JF03133

y sc v; UAS-shi.RNAiTRiP.HMS00154

w; UAS-shi.RNAiNIG.18102R-1

w; UAS-shi.K44A.3-7 (II)

w shi1

shi2

y w hs-Flp1.22; vkgG454 act-y+-GAL4 UAS-myr.RFP/CyO

y w hs-Flp1.22; act-y+-GAL4 UAS-GFP

w; UAS-Cg25C.GFP.2.1 (II)

w; UAS-Cg25C.RFP.2.1 (II)

w; UAS-hTfR.GFP (III)

y sc v; UAS-Rab11.RNAiTRiP.JF02812

w UAS-Rab11.dsRNA.WIZ

y sc v; UAS-Rab5.RNAiTRiP.HMC03420

y sc v; UAS-Rab5.RNAiTRiP.HMS00147

w; UAS-Rab5.S43N (II) (BL42703)

w; UAS-GFP.Rab5 (III) (BL43336)

y v sc; UAS-Chc.RNAiTRIP.JF02681

y v sc; UAS-Chc.RNAiTRIP.HMS01222

w; UAS-Chc.DN (III) (BL26874)

y v sc; UAS-RN-tre.RNAiTRiP.JF03085 (III)

y sc v; UAS-AP-2α.RNAiTRiP.HMS00653 (III)

y sc v; UAS-AP-2μ.RNAiTRiP.JF0287 (III)

y sc v; UAS-Hrs.RNAiTRiP.JF02860 (III)

y sc v; UAS-Par-1.RNAiTRiP.GL00253 (III)

w trolCPTI-002049

y sc v; UAS-EGFP.shRNA.3 (II) (BL41559)

y sc v; UAS-EGFP.shRNA.3 (III) (BL41560)

y sc v; UAS-EGFP.shRNA.4 (III) (BL41553)

y sc v; UAS-EGFP.shRNA (II) (BL35782) (knocks down GFP and YFP efficiently same as three above constructs; unlike those, it causes unspecific cell death in imaginal discs)

w; UAS-vkg.RNAiNIG.16858R-3 (III)

w; UAS-vkg.RNAiVDRC.v16986 (III)

w; UAS-vkg.RNAiVDRC.v106812 (II)

w; UAS-Cg25C.RNAiVDRC.v28369 (III)

w; UAS-vkg.RNAiNIG.16858R-3 UAS- Cg25C.RNAiVDRC.v28369/TM6B

w; UAS-PH4αEFB.RNAiVDRC.v2464 (III)

w; UAS-sec23.RNAiVDRC.v24552 (III)

w; 26-29-pCA06735 (III)

w; Fer1HCHG188 (III)

y sc v; UAS-cact.RNAiTRiP.GL00627 (II)

y sc v; UAS-cact.RNAiTRiP.HMS00084 (III)

cact4/CyO, act-GFP

Df(2L)r10, cn1/CyO

w; UAS-Tl10B (II)

y sc v; UAS-Dif.RNAi TRiP.HM05191 (III)

y w Drs-GFP.JM804

y sc v; UAS-Rab1.RNAiTRiP.JF02609 (III)

w; pucG462/TM6B

w; 10XSTAT-GFP (II)

UAS-Cg25C-GFP and UAS-Cg25C-RFP transgenic lines

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To obtain UAS-Cg25C-GFP and UAS-Cg25C-RFP lines, the open reading frame (ORF) of Cg25C was cloned into vectors pTWG and pTWR (Drosophila Carnegie Vector collection) using Gateway recombination (Life Technologies, Carlsbad, California). To do this, we PCR-amplified the Cg25C ORF from cDNA clone RE33133 (Drosophila Genomics Resource center, Bloomington) using primers Cg25C-NF: 5′-GGGG ACA AGT TTG TAC AAA AAA GCA GGC TTC ATG TTG CCC TTC TGG AAG CG-3′ and Cg25C-CF: 5′-GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC CGA GGA GTT CTT CAT GCA CA-3′, thus adding att sites at the 5′ and 3′ termini of the ORF for subsequent Gateway cloning. The product of this reaction was purified by gel extraction with AxyPrep DNA Gel Extraction Kit (Axygen, Union City, California) and recombined into vector pDONR221 (Life Technologies) with Gateway BP Clonase Enzyme Mix (Life Technologies). The resulting plasmid (Entry clone), was transformed into Escherichia coli Trans5α competent cells, miniprepped and recombined with destination vectors pTWG (C-terminal GFP) and pTWR (C-terminal RFP) using Gateway LR Clonase Enzyme Mix (Life Technologies). Transgenic lines were obtained through standard P-element transgenesis (Spradling and Rubin, 1982).

Immunohistochemistry

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For generation of anti-Cg25C antibody, rabbits were immunized with epitope SVKHYNRNEPKFPIDDSY by AbMax Biotechnology (Beijing, China). The following antibodies and dyes were used: rabbit anti-Cg25C (1:1,000, this study), mouse anti-rat Dynamin (1:250, BD BioScience, Franklin Lakes, New Jersey), rabbit anti-Ndg (Wolfstetter et al., 2009) (1:2000), rabbit anti-Pcan (Friedrich et al., 2000) (1:2000), rabbit anti-LanB1 (1:500, Abcam, Cambridge, UK), mouse anti-Mmp1 (Page-McCaw et al., 2003) (1:200, DSHB, University of Iowa), mouse anti-Dorsal (Whalen and Steward, 1993) (1:100, DSHB), rabbit anti-GFP (1:500, EASYBIO, Seoul, South Korea), anti-mouse IgG conjugated to Alexa-488, Alexa-555 or Alexa-633 (1:200, Life Technologies), Texas-Red phalloidin (1:100, Life Technologies) and BODIPY 493/503 (1:500, Life Technologies). Antibody stainings of whole fat body were performed following standard procedures used for imaginal discs. Briefly, larvae were pre-dissected in PBS by turning inside out with fine tip forceps after removing their posterior end. Carcasses with fat body still attached were fixed in PBS containing 4% PFA, washed in PBS (3 × 10 min), blocked in PBT-BSA (PBS containing 0.1% Triton X-100 detergent, 1% BSA and 250 mM NaCl), incubated overnight with primary antibody in PBT-BSA, washed in PBT-BSA (3 × 20 min), incubated for 2 hr with secondary antibody in PBT-BSA and washed in PBT-BSA (3 × 20 min) and PBS (3 × 10 min). Fat body tissues were finally dissected on a slide with a drop of DAPI-Vectashield (Vector Labs, Burlingame, California) and mounted in this same medium. FM4-64 staining of PM was performed by incubating fat body in ice-cold PBS containing 5 mg/ml fixable FM4-64 (Life Technologies) for 1 min before fixing for 20 min in ice-cold PBS containing 4% PFA. Labeling with fixable 3,000 and 70,000 MW Texas-Red-coupled dextran (0.1 mg/ml, Life Technologies) was performed by fixing fat body in 4% PFA for 5 min followed by three quick washes, incubation with dextran in PBS, three quick washes and fixation again for 20 min. Results were the same when the first fixation was omitted.

Imaging

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The screening was conducted in a Leica MZ10F stereoscope (Wetzlar, Germany). For confocal microscopy (Zeiss LSM780 microscope, Oberkochen, Germany), tissues were mounted in DAPI-Vectashield (Vector Labs). Vkg-GFP intensity in basement membranes was quantified with ImageJ software as previously described (Pastor-Pareja and Xu, 2011). PM depth and sinuosity measurements were performed in electron micrographs as indicated in Figure 3—figure supplement 2. For measurements of both PM depth and sinuosity, images were analyzed with ImageJ using the segmented line tool to calculate distances. Graphs were drawn and statistical analysis performed with GraphPad Prism. For sinuosity measurements, the distance separating the points where the PM intersects the edges of the micrograph was calculated, as well as the length of the PM joining those two points. Islands of cytoplasm with no connection to the cell body were excluded from sinuosity analysis, but not for PM depth measurement. All fat body images in the manuscript correspond to mid-to-late third larval instar fat body dissected prior to the wandering larva stage, except for the first and second larval instar images in Figure 3—figure supplement 1A and sec23i second instar larvae in Figure 5—figure supplement 1B. Fibrotic aggregates can be observed at all times during the third larval instar, including the extremes of early third instar and white prepupa.

Transmission electron microscopy

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Ultrathin sections were obtained following standard procedures and imaged in a Hitachi H-7650B microscope (Tokyo, Japan). Briefly, larvae were turned inside-out and fixed in 2.5% gluteraldehyde, leaving fat body attached to carcasses to facilitate handling. Once fixed, fat body was postfixed in 1% osmium tetroxide before embedding. Sections were stained in 2% uranyl acetate/lead citrate. Fat body from at least three different specimens per genotype was imaged.

Bacterial infection

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Gram+ bacterium M. luteus (ATCC 4698) was cultured in LB medium at 30°C. Larvae were soaked in concentrated culture and pricked with a tungsten needle to allow infection.

Real time RT-PCR

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RNA was isolated using TransZol Up (Transgen, Beijing, China). cDNA was synthesized from 1 μg of RNA with the PrimeScriptTM RT-PCR Kit (Takara, Kyoto, Japan). Analysis was performed in a CFX96 Touch system (Bio-Rad, Hercules, California) using SYBR Green Fast kit (Applied Biosystems, Waltham, Massachusetts). rp49 expression was used for normalization. Three experiments per genotype were averaged. A biological replicate was performed with same results. Primers used were:

upd-For: 5′ TCCACACGCACAACTACAAGTTC 3′;

upd-Rev: 5′ CCAGCGCTTTAGGGCAATC 3′;

upd2-For: 5′ AGTGCGGTGAAGCTAAAGACTTG 3′;

upd2-Rev: 5′ GCCCGTCCCAGATATGAGAA 3′;

upd3-For: 5′ TGCCCCGTCTGAATCTCACT 3′;

upd3-Rev: 5′ GTGAAGGCGCCCACGTAA 3′;

rp49-For: 5′ GGCCCAAGATCGTGAAGAAG 3′;

rp49-Rev: 5′ ATTTGTGCGACAGCTTAGCATATC 3′.

Western blots of hemolymph

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Hemolymph from third instar larvae was collected by turning larvae inside-out with fine-tip forceps while immersed in 100 μl of PBS in a glass depression well. PBS contained 0.1 mg/ml of phenylthiourea to avoid melanization and 1 mg/ml of protease inhibitor. 10 μl of 2-Mercaptoethanol-reduced sample were loaded per genotype into a 4–15% Mini-PROTEAN TGX gel (Bio-Rad). The amount of sample loaded in each experiment given in figure legends as larva-equivalents, depending on the number of larvae collected. Precision Plus Protein Dual Color Standards (Bio-Rad) was used as a molecular weight marker. Gels were blotted with rabbit anti-GFP antibody (1:5000, EASYBIO) or rabbit anti-Cg25C (1:5000, this study) and revealed with anti-rabbit-HRP (1:10,000, Abmart) and an ECL Plus kit (Pierce, Rockford, Illinois).

References

    1. Asha H
    2. Nagy I
    3. Kovacs G
    4. Stetson D
    5. Ando I
    6. Dearolf CR
    (2003)
    Analysis of Ras-induced overproliferation in Drosophila hemocytes
    Genetics 163:203–215.
    1. Brand AH
    2. Perrimon N
    (1993)
    Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
    Development 118:401–415.
    1. Fan JY
    2. Carpentier JL
    3. van Obberghen E
    4. Grunfeld C
    5. Gorden P
    6. Orci L
    (1983)
    Morphological changes of the 3T3-L1 fibroblast plasma membrane upon differentiation to the adipocyte form
    Journal of Cell Science 61:219–230.
    1. Gerttula S
    2. Jin YS
    3. Anderson KV
    (1988)
    Zygotic expression and activity of the Drosophila Toll gene, a gene required maternally for embryonic dorsal-ventral pattern formation
    Genetics 119:123–133.
    1. Ito K
    2. Awano W
    3. Suzuki K
    4. Hiromi Y
    5. Yamamoto D
    (1997)
    The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells
    Development 124:761–771.
    1. Maxfield FR
    2. McGraw TE
    (2004) Endocytic recycling
    Nature Reviews. Molecular Cell Biology 5:121–132.
    https://doi.org/10.1038/nrm1315
    1. Moline MM
    2. Southern C
    3. Bejsovec A
    (1999)
    Directionality of wingless protein transport influences epidermal patterning in the Drosophila embryo
    Development 126:4375–4384.
    1. Roth S
    2. Hiromi Y
    3. Godt D
    4. Nusslein-Volhard C
    (1991)
    Cactus, a maternal gene required for proper formation of the dorsoventral morphogen gradient in Drosophila embryos
    Development 112:371–388.

Article and author information

Author details

  1. Yiran Zang

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    YZ, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Ming Wan
    Competing interests
    The authors declare that no competing interests exist.

  2. Ming Wan

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    MW, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Contributed equally with
    Yiran Zang
    Competing interests
    The authors declare that no competing interests exist.

  3. Min Liu

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    ML, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  4. Hongmei Ke

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    HK, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  5. Shuangchun Ma

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    SM, Acquisition of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  6. Lu-Ping Liu

    1. Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, China
    2. Tsinghua Fly Center, School of Medicine, Tsinghua University, Beijing, China
    Contribution
    L-PL, Conception and design, Drafting or revising the article, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  7. Jian-Quan Ni

    Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, China
    Contribution
    J-QN, Conception and design, Drafting or revising the article, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  8. José Carlos Pastor-Pareja

    School of Life Sciences, Tsinghua University, Beijing, China
    Contribution
    JCP-P, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    jose.pastor@biomed.tsinghua.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3823-4473

Funding

National Natural Science Foundation of China (NSFC) (General program grant, 31371459)

  • José Carlos Pastor-Pareja

State Administration of Foreign Experts Affairs (1000 Young Talents award)

  • José Carlos Pastor-Pareja

Tsinghua University (Tsinghua Initiative Program, 20131089281)

  • José Carlos Pastor-Pareja

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Hugo Bellen (BM-40-SPARC-GAL4), Pierre Leopold (r4-GAL4), Herve Agaisse (ppl-GAL4), Fujian Zhang (UAS-secr-GFP) and Stefan Baumgartner (anti-Trol and anti-Ndg) for sharing fly strains and antibodies; the Bloomington Stock Center, National Institute of Genetics (Kyoto) and Vienna Drosophila RNAi Center for providing strains; the Drosophila Genomics Research Center (Indiana) for Cg25C cDNA and the Drosophila Carnegie Vector collection, and the Tsinghua Electron Microscopy facility. Xingxin Liao, Huina Jia, Jiatao Li, Xiaoxue Chen and Yuxi Li assisted in screening and some experiments. Special thanks are due to Tian Xu, in whose laboratory planning for the screening took place, and to Tatsushi Igaki and Helen Rankin for comments on the manuscript.

Copyright

© 2015, Zang et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Yiran Zang
  2. Ming Wan
  3. Min Liu
  4. Hongmei Ke
  5. Shuangchun Ma
  6. Lu-Ping Liu
  7. Jian-Quan Ni
  8. José Carlos Pastor-Pareja
(2015)
Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes
eLife 4:e07187.
https://doi.org/10.7554/eLife.07187

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https://doi.org/10.7554/eLife.07187

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