Figure 1—figure supplement 2. | Human kinetochores are swivel joints that mediate microtubule attachments

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Human kinetochores are swivel joints that mediate microtubule attachments

Figure 1—figure supplement 2.

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University of Warwick, United Kingdom
Figure 1—figure supplement 2.
Download figureOpen in new tabFigure 1—figure supplement 2. Intra-kinetochore distances with and without microtubule attachment.

Schematic of the 3D mammalian kinetochore with (top) and without (bottom) attachment to a kinetochore-microtubule (kMT). The difference between endogenous CENP-A and eGFP-CENP-A has been attributed to limitations of antibody accessibility to CENP-A that is deep in centromeric chromatin (Magidson et al., 2016). As previously found (Suzuki et al., 2015) the majority of CENP-C amino-termini are 'free' and in close proximity of the centromere, rather than directly bound to the 20 nm long MIS12 complex (Petrovic et al., 2014). The orientation of the NDC80 complex is suggested based on the 60 nm length of the complex and the flexibility in the hinge region (Wei et al., 2005; Wang et al., 2008). Bub3 binds to kinetochores through the phospho-MELT repeats in KNL1 suggesting that this protein extends beyond the NDC80 complex – at least during metaphase.

DOI: http://dx.doi.org/10.7554/eLife.16159.005