Figure 6—figure supplement 1. | A long-term epigenetic memory switch controls bacterial virulence bimodality

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A long-term epigenetic memory switch controls bacterial virulence bimodality

Figure 6—figure supplement 1.

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The Hebrew University of Jerusalem, Israel
Figure 6—figure supplement 1.
Download figureOpen in new tabFigure 6—figure supplement 1. Expression of perABC in wild-type EPEC during activation.

(A) Expression of perABC in a wild-type EPEC population grown in activating conditions was analyzed using a transcriptional GFP reporter for perABC (wt/pPerABC-GFP). Activated culture was placed on LB agar pads (non-activating conditions. i.e. LB agar at 32°C) and analyzed by time-lapse microscopy. Similar results were obtained in at least five different locations and in two independent biological replicates. (B) A wild type EPEC control strain, containing a plasmid expressing GFP from PLtetO-1 promoter (wt/ pZS11*GFP) did not display bimodal GFP expression under the same conditions. Similar results were obtained in at least five technical replicates. Scale bars: 15 μm.

DOI: http://dx.doi.org/10.7554/eLife.19599.014