HNF1A, a risk factor gene for pancreatic ductal adenocarcinoma, is critical to maintaining pancreatic cancer stem cell properties through regulating POU5F1 (OCT4) expression, providing a novel role for HNF1A in maintenance of the disease.

Editors' Summary: This Replication Study has reproduced important parts of the original paper.

Insights into the roles and mechanisms of two of the miRNAs upregulated in human breast cancer stem cells in at least some breast cancers are presented.

Chromatin-based cancer stem cell heterogeneity typifies primary glioblastoma and includes an invasive subtype associated with a lower survival.

Genetic lineage tracing approach combined with live imaging visualized the functional stem cell activity of Doublecortin-like kinase 1⁺ cells within pancreatic cancer in vivo.

A novel long non-coding RNA targets microRNA miR-34a for epigenetic silencing and initiates asymmetric division of colon cancer stem cells.

A new mechanism connects the loss of TGFβ signaling with invasion and metastasis in highly malignant transition zone tumors.

Cancer stem cells that have undergone EMT are identified in the region surrounding metastatic human oral tumours in a retrospective cohort study, and these are predictive of metastatic outcome in an image-based machine learning approach.

Editors' Summary: This Replication Study did not reproduce those experiments in the original paper that it attempted to reproduce.

Analyses of genetically engineered mouse models reveal the androgen receptor-independent properties of a luminal stem/progenitor cell in the prostate epithelium, and its ability to serve as a cell of origin for castration-resistant prostate cancer.