Fluorescent glutamate indicators distort the time course of neurotransmitter diffusion and uptake by competing with transporters, an important caveat to consider when using iGluSnFR and its analogs.

Photobleaching and recovery of a fluorescent glutamate sensor enable demonstration of the distinct spatial architectures of spontaneous versus evoked forms of glutamatergic neurotransmission within single synapses.

Two-photon glutamate imaging reveals that 'sustained' and 'transient' input are systematically arranged along single starburst dendrites, supporting the 'space-time' wiring model for direction selectivity.

The calcium-binding proteins synaptotagmin 7 and Doc2α act sequentially during asynchronous neurotransmitter release.

Synaptotagmin 7 is asymmetrically localized to the axonal plasma membrane through γ-secretase processing to promote vesicle docking during short-term synaptic plasticity in mouse hippocampal neurons.

Single bouton measurements of calcium influx shows that activating GABA_B receptors at nerve terminals can lead to complete inhibition of calcium entry when one is operating at the physiological setpoint of extracellular calcium concentration.

Targeting a genetically-encoded glutamate sensor to postsynaptic sites demonstrates the feasibility of simultaneous optical quantal analysis of many inputs to a neuron in wide-field fluorescence microscopy.

A genetically encoded fluorescent biosensor reveals temporally resolved changes to aspartate concentration during genetic, pharmacological, and nutritional manipulations.

Fluorescent glutamate sensors shed light on the microscopic organization underlining spontaneous neurotransmission.

Knockoff is a tool for researchers who wish to acutely disrupt a membrane protein.