Unconventional secretion of a plasma membrane purine transporter via Golgi-bypass is established at an early ER-associated secretory compartment revealing that specific cargoes define alternative trafficking routes.

Electron tomography and biochemical approaches demonstrate a direct role for WDR35, beyond integrity of the IFT-A holocomplex, in the formation and fusion of electron-dense-coated vesicles to the ciliary sheath and pocket for delivery of cargos necessary for axoneme elongation.

A human endogenous fusogen, syncytin, which facilitates placental cell-cell fusion during early embryonic development, mediates the formation of open-ended membrane tubular connections between other cell types that efficiently promotes the intercellular transfer of cytoplasmic cargos including Cas9 protein and organelles.

Oligomerization of p62/SQSTM1 generates high avidity binding regions that enable it to spatially select for concentrated ubiquitin and ATG8-family proteins.

Efficient nuclear transport of very large biomolecules, relevant for viral transport, scales non-linearly with size and its kinetics can be explained by a simple two-parameter energetic model.

A structure of the complete, membrane bound, COPII coat solved by sub-tomogram averaging reveals the arrangement of all protein subunits on the membrane and suggests a mechanism for coating heterogeneously-shaped carriers.

The soluble cargo protein albumin traverses the Golgi stack at much faster rates than procollagen aggregates, by diffusion via intercisternal continuities.

During tracheal morphogenesis, the scavenger receptor Emp senses and responds to luminal cargo levels by initiating apical membrane endocytosis along the longitudinal tube axis to restrict airway elongation.

A catabolic cascade is essential to enter a stable G1/G0 quiescent state and survive extended periods of starvation.

The same membrane trafficking cargo can change pathways mediated by different members of ARF-GEF family of vesicle formation regulators.