Microbial lineage tracing during murine systemic infection with Salmonella uncovered the bottlenecks that restrict colonization and the hidden routes of interorgan dissemination that drive heterogeneity in infection outcomes.

Zinc transporter Slc30a1 mediates antibacterial defense in macrophages.

Bacillus velezensis has broad-spectrum antibacterial activity and can reduce the colonization of Salmonella Typhimurium in the intestinal tract of mice.

Enteric bacteria associated with bloodstream infections are attracted to human serum through L-serine and the chemoreceptor Tsr, and in an enterohemorrhagic lesion model use chemotaxis to invade damaged vasculature.

A signal for inflammatory cell death is induced by the disruption of cellular metabolism.

A response regulator acquired via lateral gene transfer has rewired an ancestral genetic circuitry and regulates alternative pathogenic lifestyles.

Real-time visualization of Salmonella enterica and polarized macrophage interaction in the living host identifies an anti-inflammatory, pro-regenerative, and motionless macrophage subset as a survival niche during persistent infection.

The application of deep learning fills a major gap in host–pathogen research providing for unbiased, multi-module high-throughput image analysis.

SIRT1 and SIRT3-mediated immunometabolic shift governs intracellular Salmonella proliferation both in vitro and in vivo and dissemination in mice, thereby influencing the infection outcomes.

A single conserved residue – histidine 12 – confers pH-sensitive DNA binding to the Salmonella Typhimurium SPI-2 transcriptional regulator SsrB, thereby regulating Salmonella virulence.