The articles in this special issue highlight how modern cellular, biochemical, biophysical and computational techniques are allowing deeper and more detailed studies of allosteric kinase regulation.
Carlo Giannangelo, Matthew P Challis ... Darren J Creek
M1 alanyl aminopeptidase is chemically validated as an attractive antimalarial target by confirming the on-target activity of a novel selective inhibitor using chemoproteomics and metabolomics-based drug target deconvolution approaches.
The first structural and biochemical study of the role of the ATP-cone in anaerobic ribonucleotide reductases shows that dATP allosterically prevents transfer of a glycyl radical to the substrate.
Bacterial exonuclease III exhibits underestimated enzymatic activities on single-stranded DNA, which may redefine its roles in relevant biological processes and biosensor development.
A biochemical approach shows co-phase separation of CCL5 with heparan sulfate establishes a specific chemokine concentration gradient for chemotactic activity.
Laurie Peverini, Sophie Shi ... Pierre-Jean Corringer
Conformational motions elicited by antagonists, partial agonists, and agonists in serotonin-gated ion channels 5-HT3AR progressively spread from the extracellular to the transmembrane domain.
In addition to the exon-F-binding domain and the globular tail domain-binding motif, the actin-binding domain of melanophilin interacts with myosin-5a tail region and mediates the association of myosin-5a with melanosomes.
Roberto Efraín Díaz, Andrew K Ecker ... James S Fraser
Biochemical and structural investigations of the pH adaptability of mouse Acidic Mammalian Chitinase offer insights for designing enhanced enzyme variants that can function in both lung and gut.
