Carlo Giannangelo, Matthew P Challis ... Darren J Creek
M1 alanyl aminopeptidase is chemically validated as an attractive antimalarial target by confirming the on-target activity of a novel selective inhibitor using chemoproteomics and metabolomics-based drug target deconvolution approaches.
The first structural and biochemical study of the role of the ATP-cone in anaerobic ribonucleotide reductases shows that dATP allosterically prevents transfer of a glycyl radical to the substrate.
Bacterial exonuclease III exhibits underestimated enzymatic activities on single-stranded DNA, which may redefine its roles in relevant biological processes and biosensor development.
A biochemical approach shows co-phase separation of CCL5 with heparan sulfate establishes a specific chemokine concentration gradient for chemotactic activity.
Laurie Peverini, Sophie Shi ... Pierre-Jean Corringer
Conformational motions elicited by antagonists, partial agonists, and agonists in serotonin-gated ion channels 5-HT3AR progressively spread from the extracellular to the transmembrane domain.
In addition to the exon-F-binding domain and the globular tail domain-binding motif, the actin-binding domain of melanophilin interacts with myosin-5a tail region and mediates the association of myosin-5a with melanosomes.
Roberto Efraín Díaz, Andrew K Ecker ... James S Fraser
Biochemical and structural investigations of the pH adaptability of mouse Acidic Mammalian Chitinase offer insights for designing enhanced enzyme variants that can function in both lung and gut.
Direct inhibition of pyridoxal phosphatase activity by 7,8-dihydroxyflavone indicates a pharmacological entry point into brain disorders associated with vitamin B6 deficiency.
