A dedicated structural element facilitates chaperone and antigenic peptide selector function of TAP-associated glycoprotein, a major quality assurance auditor in adaptive immunity.
Xinru Wang, Dimitriya H Garvanska ... Rebecca Page
A novel dynamic charge-charge interaction between B56 and a subset of PP2A-B56 substrates is essential for substrate specificity, dephosphorylation and, for KIF4A, binding condensin I.
The intrinsically disordered N-terminus of Sfr1 contains two Rad51 binding sites that facilitate Rad51 filament stabilization and ATPase stimulation by the Swi5-Sfr1 complex, leading to efficient Rad51-driven strand exchange.
Andrea Graziadei, Frank Gabel ... Teresa Carlomagno
Integrative structural biology reveals a conformational equilibrium in the Box C/D methylation enzyme that regulates the extent of site-specific 2'-O-rRNA methylation in dependence of the RNA sequence.
Nilkanth Patel, Xi Ping Huang ... Vsevolod Katritch
Large scale virtual screening using recently solved structures of Melatonin receptors yield discovery of 10 new high-affinity selective agonists, also revealing novel functional features, including biased signaling at Melatonin receptors.
Fusion proteins composed of the Sleeping Beauty transposase and catalytically inactive Cas9 target transposon integration into genomic regions specified by single guide RNAs.
The mitochondrial protein unfoldase ClpX activates the first enzyme in heme biosynthesis, ALAS, by targeted unfolding that gates access of cofactor to the ALAS active site.
Lindsey RF Backman, Yolanda Y Huang ... Catherine L Drennan
The common post-translational modification trans-4-hydroxy-L-proline is reversed by gut microbes with the help of hydroxyproline dehydratase (HypD), an enzyme that performs a radical chemical mechanism.
Novel transgenic zebrafish lines allow selective ablation of neuronal mitochondria by far red light, providing a powerful tool for investigating mitochondrial homeostasis in neurons and mitochondrial mechanisms in neurological disease.
