Theoretical analysis of the current literature reveals the structural assembly, transport regulation, and molecular functions of the BBSome, emphasizing its roles in cilium-related processes.
Live imaging and physical perturbations of spindles with unfocused poles reveal that mammalian spindle length emerges locally from kinetochore-fibers, but that global cues from focused spindle poles are needed to coordinate k-fibers across space and time and accurately segregate chromosomes.
Eike K Mahlandt, Sebastián Palacios Martínez ... Joachim Goedhart
Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.
A genetic approach in Caenorhabditis elegans shows that the cleaved and cytosolic form of the ubiquitin-like protein LGG-1/GABARAP is sufficient for both autophagy and embryonic development.
Specific glutamylation of some α-tubulin isotypes affects kinesin-1 localization and transport processes that depend on it, but their absence can speed up transport, possibly explaining why some large cells express α-tubulin isotypes that are not glutamylated.
Courtney A Copeland, Benjamin A Olenchock ... William M Oldham
Hypoxia does not increase glycolysis in proliferating primary cells and antagonizes the increase in glycolysis caused by activation of hypoxia-inducible factor in normoxia, in part, through activation of MYC signaling pathways.
