Muscle contraction is required to maintain the pool of muscle progenitors via YAP and NOTCH during fetal myogenesis
Abstract
The importance of mechanical activity in the regulation of muscle progenitors during chick development has not been investigated. We show that immobilization decreases NOTCH activity and mimics a NOTCH loss-of-function phenotype, a reduction in the number of muscle progenitors and increased differentiation. Ligand-induced NOTCH activation prevents the reduction of muscle progenitors and the increase of differentiation upon immobilization. Inhibition of NOTCH ligand activity in muscle fibers suffices to reduce the progenitor pool. Furthermore, immobilization reduces the activity of the transcriptional co-activator YAP and the expression of the NOTCH ligand JAG2 in muscle fibers. YAP forced-activity in muscle fibers prevents the decrease of JAG2 expression and the number of PAX7+ cells in immobilization conditions. Our results identify a novel mechanism acting downstream of muscle contraction, where YAP activates JAG2 expression in muscle fibers, which in turn regulates the pool of fetal muscle progenitors via NOTCH in a non-cell-autonomous manner.
https://doi.org/10.7554/eLife.15593.001eLife digest
Skeletal muscle is attached to the skeleton and allows the body to move. Making a new muscle, or repairing an existing one, relies on stem cells that are present inside muscles. A major goal of skeletal muscle research is to understand the signals that regulate the abilities of muscle stem cells to divide and give rise to more stem cells or to become muscle cells. Molecular signals are known to regulate the numbers of stem cells in the muscle. Skeletal muscles become larger if they are exercised, but it is not clear if mechanical forces generated by muscle contractions directly affect the number of muscle stem cells.
The NOTCH signaling pathway contributes to maintaining the population of stem cells in muscles by forcing the stem cells to divide and preventing them from becoming muscle cells. Here, Esteves de Lima et al. investigated whether muscle contraction regulates NOTCH signaling during muscle formation in chick fetuses.
The experiments show that muscle contraction stimulates the activity of a protein called YAP in muscle cells, which in turn, activates a gene in the NOTCH signaling pathway known as JAG2. This increases NOTCH signaling activity in the neighboring stem cells and maintains the number of stem cells in the muscle. The next step following this work will be to establish if this mechanism also operates during muscle formation and regeneration in other animals such as mice and zebrafish.
https://doi.org/10.7554/eLife.15593.002Introduction
Skeletal muscle development, growth and regeneration rely on muscle stem cells. A major goal of muscle research is to understand the signals that regulate the ability of stem cells to self-renew or differentiate.
Skeletal muscle formation involves successive and overlapping phases of embryonic, fetal, perinatal and adult myogenesis. The paired homeobox transcription factors, PAX3 and PAX7, define the pool of muscle stem cells during developmental, postnatal and regenerative myogenesis (Gros et al., 2005; Kassar-Duchossoy, 2005; Relaix et al., 2005). Fetal myogenesis depends on PAX7-expressing muscle progenitors and is associated with muscle growth (Hutcheson et al., 2009; Kassar-Duchossoy, 2005; Relaix et al., 2005). Muscle progenitors undergo myogenic differentiation program with the activation of the bHLH Myogenic Regulatory Factors (MRFs), MYF5, MRF4, MYOD, MYOG (Tajbakhsh, 2009). By the end of fetal myogenesis, PAX7+ cells adopt a satellite cell position under the basal lamina of muscle fibers (Biressi et al., 2007; Bröhl et al., 2012). During development, mechanical forces generated by muscle contraction are essential for the correct establishment of the musculoskeletal system. Although the influence of the mechanical forces for cartilage, joint, and bone development has been previously addressed (Nowlan et al., 2010; Rolfe et al., 2014; Shwartz et al., 2013), the consequence of muscle-induced mechanical load for the development of muscle itself is largely unknown.
The NOTCH signaling pathway is a central regulator of skeletal muscle stem cells during embryonic, fetal and adult myogenesis [reviewed in Mourikis and Tajbakhsh (2014)]. Activation of the NOTCH signaling pathway requires direct cell-cell contact between a signal-sending cell that expresses the NOTCH ligand and a signal-receiving cell that expresses the NOTCH receptor. Upon ligand activation, the intracellular domain of the NOTCH receptor is cleaved, translocates into the nucleus, associates with the transcription factor RBPJ and activates the transcription of the bHLH transcriptional repressor genes, HES and HEY [reviewed in Andersson et al. (2011)]. In adult myogenesis, NOTCH is involved in satellite cell activation, proliferation and quiescence [reviewed in Mourikis and Tajbakhsh (2014)] and the absence of NOTCH signaling in muscle stem cells results in satellite cell depletion due to premature differentiation (Bjornson et al., 2012). In addition, during development, NOTCH has been described to activate embryonic myogenesis in somites (Rios et al., 2011). During developmental myogenesis, active NOTCH signaling is associated with proliferating muscle progenitors, while NOTCH ligands are expressed in differentiated muscle cells (Delfini et al., 2000; Vasyutina et al., 2007). NOTCH loss-of-function experiments in mice induce a loss of the muscle progenitor pool due to premature muscle differentiation (Bröhl et al., 2012; Czajkowski et al., 2014; Schuster-Gossler et al., 2007; Vasyutina et al., 2007), whereas NOTCH activation represses muscle differentiation in chick and mouse embryos (Delfini et al., 2000; Hirsinger et al., 2001; Mourikis et al., 2012). While studies have identified NOTCH target genes in fetal muscle progenitors (Bröhl et al., 2012; Mourikis et al., 2012), upstream regulators of NOTCH signaling during developmental myogenesis have not attracted attention.
Similarly to NOTCH, the co-transcriptional activator YAP (Yes-Associated Protein) promotes satellite cell proliferation and inhibits muscle differentiation in culture (Judson et al., 2012; Watt et al., 2010). In addition to being a nuclear effector of the Hippo pathway, YAP has been identified as a sensor of mechanical activity and mediates cellular and transcriptional responses downstream of mechanical forces (Aragona et al., 2013; Dupont et al., 2011; Porazinski et al., 2015). In addition to other transcription factors, YAP binds to TEAD DNA binding proteins (Varelas, 2014). YAP and TEAD1 have been shown to occupy 80% of the same promoters in human mammary epithelial cells (Zhao et al., 2008), indicating that YAP/TEAD interaction could constitute the major molecular mechanism of YAP-mediated regulation of gene transcription. The TEAD transcription factors recognize and bind to MCAT elements (CATTCC), which are enriched in regulatory regions of muscle-related genes [reviewed in Wackerhage et al. (2014)]. In addition to being involved in muscle stem cell proliferation (Judson et al., 2012; Watt et al., 2010), YAP has been recently shown to be a critical regulator of skeletal muscle fiber size in adult mice (Goodman et al., 2015; Watt et al., 2015).
A link between mechanical forces (provided by muscle contraction) and signaling pathways that regulate fetal myogenesis has not been established. In this study, we show the importance of mechanical forces in the regulation of the number of fetal muscle progenitors. We show that immobilization induces a NOTCH loss-of-function phenotype in muscles. We further provide evidence that, downstream of mechanical forces, YAP positively regulates the expression of the NOTCH ligand JAG2 in fibers, which maintains the pool of muscle progenitors by activating NOTCH signaling.
Results
Inhibition of muscle contraction reduces the number of progenitors and increases their differentiation in fetal muscles
To study the effect of mechanical signals on muscle progenitors, we set up an unloading model during chick fetal myogenesis (Figure 1A). We used decamethonium bromide (DMB), which blocks muscle contraction and induces rigid paralysis that leads to immobilization (Nowlan et al., 2010). Two days after the inhibition of muscle contraction, we observed a reduction in the overall muscle size (Figure 1D,H), which is consistent with previous reports (Crow and Stockdale, 1986; Hall and Herring, 1990). In addition, we observed a decrease of 58.07% (±17.66) in the number of PAX7+ muscle progenitors in paralyzed compared to control muscles (Figure 1B,D-F,H-J). Consistently, the relative expression levels of PAX7 and MYF5 genes were significantly decreased in DMB-treated limbs compared to control limbs, as early as 12 hr after DMB application and with a more prominent effect at 48 hr (Figure 1C). In addition to the reduction of the muscle progenitor pool, we also observed an increase of myogenic differentiation assessed by an increase of MYOD and MYOG expression using RT-q-PCR or in situ hybridization in immobilized limbs compared to control limbs (Figure 1C,G,K). The number of MYOD-expressing cells was also increased in paralyzed muscles compared to control muscles (Figure 1M). In addition, muscle fibers were larger in limb muscles of DMB-treated fetuses compared to control muscles (Figure 1E,I). The large muscle fibers were associated with several nuclei in paralyzed muscles, while control muscle fibers displayed only one nucleus on transverse sections (Figure 1L). Injection of pancuronium bromide (PB), a drug that exerts a flaccid skeletal muscle paralysis (Nowlan et al., 2010) led to a similar but less pronounced effect, i.e. a 28.92% (±7.27) reduction in the number of PAX7+ cells and a concomitant increase of muscle differentiation (Figure 1—figure supplement 1). DMB or PB treatments of chick fetal myoblast cultures did neither affect muscle progenitors nor their differentiation and did not change the expression levels of PAX7, MYF5, MYOD and MYOG (Figure 1—figure supplement 2), indicating that DMB and PB did not have any off-target effect on myogenic cells. We conclude that the inhibition of muscle contraction leading to rigid or flaccid paralysis reduces the pool of fetal muscle progenitors and increases their propensity to differentiate.
NOTCH activity is decreased in muscles of immobilized fetuses
The concomitant decrease of the muscle progenitor pool and increase of muscle differentiation following muscle paralysis was reminiscent of a NOTCH loss-of-function phenotype. In the murine system, loss of NOTCH signaling results in a reduction of the progenitor pool due to a precocious shift toward differentiation (Bröhl et al., 2012; Vasyutina et al., 2007). To determine whether NOTCH activity was modified upon immobilization, we examined the expression of components of the NOTCH signaling pathway in paralyzed muscles. During fetal myogenesis, the NOTCH ligand JAG2 was expressed in MF20+ differentiated muscle cells (Delfini et al. 2000), while HES5 a recognized transcriptional readout of NOTCH activity (Andersson et al., 2011) was excluded from differentiated muscle fibers (Figure 2A,D). The NOTCH ligand DLL1 is expressed during chick and mouse embryonic myogenesis (Vasyutina et al. 2007, Delfini et al., 2000) but is not detected by in situ hybridization in chick limb fetal muscles (Delfini et al., 2000). JAG2 and HES5 were also expressed in blood vessels (Figure 2B,E, arrowheads). In immobilized fetuses, the expression of the JAG2 and HES5 was decreased in paralyzed muscles (Figure 2C,F,I) compared to control muscles (Figure 2B,E,H). We believe that the downregulation of JAG2 and HES5 expression in paralyzed muscles reflected a muscle-specific loss of gene expression, since JAG2 and HES5 expression was not affected in blood vessels (Figure 2B,C,E,F,H,I, arrowheads). Moreover, blood vessels are surrounding fetal muscles in normal conditions (Figure 2J,K), when muscle splitting is accomplished (Tozer et al., 2007). Consistently, the JAG2 and HES5 mRNA levels were moderately but significantly downregulated in limbs of immobilized animals (Figure 2G). We believe that the unchanged JAG2 and HES5 expression in blood vessels obscures the changes in JAG2/HES5 expression levels in muscles. HeyL is another transcriptional readout of NOTCH that responds to NOTCH activation in limb fetal muscle cells in mice (Mourikis et al., 2012; Bröhl et al., 2012). The relative mRNA levels of HEYL gene were also significantly downregulated in limbs of immobilized animals compared to controls (Figure 2G). In summary, NOTCH activity was reduced in paralyzed muscles, as assessed by reduced expression of the NOTCH ligand JAG2 in muscle fibers and of the transcriptional readout of NOTCH activity, HES5, in muscles. The downregulation of NOTCH signaling (Figure 2) and the concomitant reduction of the muscle progenitor pool and increase of differentiation (Figure 1, Figure 1—figure supplement 1) observed in unloading conditions led us to conclude that immobilization mimics a NOTCH loss-of-function phenotype in muscles.
The absence of muscle contraction does not affect muscle progenitor proliferation
As the pool of muscle progenitors was decreased after muscle paralysis (Figure 1), we assessed proliferation and apoptosis of muscle progenitors by EdU incorporation and TUNEL analyses in immobilization conditions (Figure 3). EdU incorporation into PAX7+ cells was not altered, indicating that the proliferative rate of PAX7+ cells was not changed during immobilization compared to control conditions (Figure 3A–G). Despite the reduced number of PAX7+ cells in paralyzed muscles (Figure 1), their proliferation capacities were unaffected (Figure 3A–G). While apoptotic cells were very rare in control muscles (Figure 3H,J,L,N), we observed an increase of apoptotic figures in fetal muscles of immobilized animals, 48 hr after DMB treatment (Figure 3I,K,M,O). We found no increase of apoptosis 24 hr after DMB treatment (data not shown). The apoptotic figures were not observed in PAX7+ cells, 48 hr after DMB treatments (Figure 3H–K), nor in MF20+ cells or in Desmin+ cells (Figure 3J–O). The absence of apoptotic figures in myogenic cells suggests that cells undergoing apoptosis upon immobilization are possibly muscle connective tissue cells. Thus, immobilization did neither affect the proliferative capacity nor apoptosis of PAX7+ cells, which was reminiscent of the unchanged proliferation and apoptosis rate of muscle progenitors in NOTCH loss-of-function in mice (Vasuytina et al., 2007).
Ligand-mediated activation of NOTCH prevented the loss of muscle progenitors in the absence of muscle contraction
The inhibition of muscle contraction mimicked a NOTCH loss-of-function phenotype in fetal muscles (Figures 1–3). To determine whether NOTCH signaling acts downstream of mechanical signals to regulate the muscle progenitor pool, we performed NOTCH rescue experiments in immobilization conditions. DLL1-induced NOTCH activation in chick embryos inhibits muscle differentiation (Delfini et al., 2000; Hirsinger et al., 2001). We first performed ligand-dependent NOTCH activation and analyzed the consequences for PAX7+ cells in normal conditions of muscle activity. In DLL1-activated NOTCH limbs, the pool of PAX7+ cells was maintained at E9.5, despite inhibition of muscle differentiation (Figure 4—figure supplement 1), which is consistent with the maintenance of PAX7+ progenitors in NICD-expressing cells in mouse fetuses (Mourikis et al., 2012). Thus, ligand-activated NOTCH maintained the number of chick fetal muscle progenitors over time and impaired their differentiation. We then forced NOTCH activity in limbs of immobilized fetuses and analyzed the consequences for muscle using the contralateral non-grafted limbs as control (Figure 4). Under immobilization conditions, the left limbs displayed a reduction in progenitor numbers and increased differentiation (Figure 4A,C,E,G). In contrast, in DLL1-expressing regions of (right) limbs, the number of PAX7+ cells was increased by 215.81% (± 29.36) and myosin expression was decreased compared to control (left) limbs (Figure 4A–I). Since immobilization leads to a 2.3-fold (58.07%) reduction in the number of PAX7+ cells (Figure 1B), we estimated that retroviral DELTA1-induced NOTCH rescued around 93% the number of PAX7+ cells in paralyzed muscles. We conclude that NOTCH activation prevents the decrease in the number of PAX7+ cells by preventing their inappropriate differentiation under immobilization conditions.
NOTCH ligand activity in differentiated muscle cells is required to maintain the pool of muscle progenitors
NOTCH signaling depends on the direct contact between signal-sending cells that express the NOTCH ligands and signal-receiving cells that express NOTCH receptors and display active NOTCH (Andersson et al., 2011). Since we observed a concomitant loss of the expression of the NOTCH ligand JAG2 in muscle fibers and reduced expression of NOTCH target genes in paralyzed muscles (Figure 2), it was unclear which cell type, between differentiated fibers and progenitors, first sensed muscle contraction. To test whether the loss of NOTCH ligand in muscle fibers would suffice to reduce the size of the muscle progenitor pool, we blocked NOTCH ligand function specifically in muscle fibers. For this, we overexpressed a dominant-negative form of DELTA1 (DELTA1/DN) (Figure 5A), which prevents NOTCH ligand processing in signal-sending cells and therefore blocked NOTCH activation in signal-receiving cells (Chitnis, 2006; Henrique et al., 1997). We performed chick somite-electroporation at the forelimb level (Figure 5B) using a stable vector that can be integrated into the genome in the presence of a transposase (Bourgeois et al., 2015). This vector co-expresses the Tomato reporter gene and DELTA1/DN under the control of the mouse Myosin Light Chain (MLC) promoter that drives expression in chick-differentiated muscle cells and not in muscle progenitors (Wang et al., 2011). In electroporated Tomato-expressing muscles, 44.94% (±12.88) of MF20+ cells displayed Tomato expression (Figure 5D–E). We observed that the lack of ligand activity in around 45% of differentiated muscle cells significantly decreased the number of PAX7+ cells by 32.42% (±7.23) compared to contralateral limbs (Figure 5F–K). This shows that a diminution of NOTCH ligand activity in muscle fibers suffices to induce a decrease in the number of PAX7+ cells. We conclude that NOTCH ligand activity in muscle fibers is required to maintain the pool of fetal muscle progenitors.
YAP activity is observed in differentiated muscle fibers and is decreased in paralyzed muscles
We next aimed to identify the signal that could sense mechanical forces and regulate JAG2 expression in muscle fibers. We focused on the transcriptional co-activator YAP that has been shown to sense mechanical signals independently of the Hippo pathway (Aragona et al., 2013; Dupont et al., 2011). YAP1 transcripts and YAP protein were expressed ubiquitously in chick limbs (Figure 6—figure supplement 1A–E). Since the subcellular localization of YAP protein reflects its transcriptional activity (Dupont et al., 2011), we examined nuclear YAP staining in MF20+ and PAX7+ cells. Unexpectedly, given the proliferative role of YAP in organ formation and tumorigenesis (Zanconato et al. 2015, Piccolo et al., 2014), we found that 89.6% (±3.65) of myonuclei of post-mitotic MF20+ cells displayed nuclear YAP protein (Figure 6A-C,G,H; Figure 6—figure supplement 1F,G). Moreover, YAP nuclear staining was stronger than YAP cytoplasmic staining in MF20+ cells; the cytoplasmic domains being delineated with MF20 staining (Figure 6G, Figure 6—figure supplement 1F). In contrast, only a subset of PAX7+ cells showed nuclear YAP staining (Figure 6—figure supplement 1H). Myoblast proliferation has been shown to be associated with increased nuclear YAP in cell cultures (Judson et al., 2012; Watt et al., 2010). However, we did not detect an obvious correlation between nuclear YAP protein and the proliferative state of PAX7+ cells in chick limb fetal muscles, in vivo (Figure 6—figure supplement 1I). ANKRD1 and CTGF are two recognized direct target genes of YAP in many cell types (Lai et al., 2011; Zhao et al., 2008). ANKRD1 is a muscle ankyrin-repeat protein that binds sarcomeric proteins (Kojic et al., 2011), and CTGF is a secreted matricellular protein involved in multiple cellular processes (Malik et al., 2015). ANKRD1 and CTGF expression was observed at the tips of MF20+ muscle fibers (Figure 6I-M,Q). In fetal limbs, ANKRD1 was exclusively expressed in muscle fibers (Figure 6I,K,L), whereas CTGF transcripts were observed also in cartilage (Figure 6M,Q). YAP staining in myonuclei and the expression of YAP target genes in muscle fibers show that YAP is active in differentiated muscle cells of chick fetal limbs. The regionalized location of the YAP target gene transcripts at muscle tips along fibers suggests that additional proteins are regionalized at muscle tips to regulate ANKRD1 and CTGF transcription. In immobilized fetuses, ANKRD1 and CTGF expression was lost in muscle fibers (Figure 6I–R), but CTGF expression was maintained in cartilage (Figure 6M,P–R). Consistently, the mRNA levels of ANKDR1 showed a drastic diminution, while those of CTGF displayed a significant but less strong reduction in immobilization conditions (Figure 6S). CYR61 is another matricellular protein of the same family as CTGF and is also a YAP target gene (Lai et al., 2011). CYR61 mRNA levels were also decreased in limbs 48 hr after DMB treatment (Figure 6S). Moreover, the number of YAP+ myonuclei was significantly decreased in muscle fibers of paralyzed muscles compared to control muscles (dropping from 89.6% ± 3.66 to 15.9% ± 4.36) (Figure 6H). We did not observe any obvious modification of nuclear YAP staining in PAX7+ cells of DMB-treated versus control animals (Figure 6—figure supplement 1J,K), indicating that the nuclear YAP staining in the subpopulation of PAX7+ cells is unrelated to mechanical signals. We conclude that YAP activity is observed in muscle fibers of contracting muscles and that this activity is lost in paralyzed muscles.
YAP forced-activity in differentiated muscle cells rescues JAG2 expression and the muscle phenotype in paralyzed muscles
The concomitant loss of YAP activity and JAG2 expression in muscle fibers of paralyzed muscles prompted us to test whether YAP controlled JAG2 expression. We overexpressed chick fetal myoblasts in a constitutively active form of mouse Yap that cannot be phosphorylated at Ser112 and is therefore preferentially translocated to the nucleus (Xin et al., 2013). Transfection of YapS112A/RCAS into fetal myoblasts increased the expression levels of YAP target genes (CTGF and CYR61) (Figure 7—figure supplement 1A). In addition, we also observed changes in muscle gene expression, previously described in C2C12 cells and satellite cell-derived myoblasts (Judson et al., 2012; Watt et al., 2010), i.e. concomitant increase of PAX7 and MYF5 and reduction of MYOD and MYOG expression. YapS112A also activated JAG2 expression and NOTCH target genes (Figure 7—figure supplement 1A). JAG2 expression was also upregulated in differentiated muscle cells after transfection with MLC-Tomato-YapS112A (Tomato+ FACS-sorted cells) (Figure 7—figure supplement 1B). Thus, YAP positively regulated JAG2 expression in cultured fetal muscle cells. In order to assess whether YAP would rescue JAG2 expression and the muscle phenotype observed in immobilization conditions, in vivo, we electroporated the MLC-Tomato-YapS112A construct in chick limb somites of embryos that were then treated with DMB. We observed that YAP forced-activity in differentiated muscle cells activated ANKRD1 expression in paralyzed muscles compared to the loss of ANKRD1 in paralyzed muscles (Figure 7A,B,D,E). This shows that YapS112A activates YAP target gene expression in paralyzed muscles. YAP forced-activity also rescued JAG2 expression in paralyzed muscles compared to the loss of JAG2 in paralyzed muscles (Figure 7A,C,D,F). Moreover, the number of PAX7+ cells was increased of 55.19% (± 10.2) in muscles displaying YapS112A expression in differentiated muscle cells (visualized with Tomato expression) compared to muscles of contralateral limbs (Figure 7G–N). We conclude that YAP forced-activity in muscle fibers prevents the loss of JAG2 expression in muscle fibers and the decrease in the number of adjacent muscle progenitors in immobilization conditions.
YAP is recruited to JAG2 promoter in chick limb muscles
To define whether YAP directly regulates JAG2 transcription, we performed in vivo ChIP experiments using chick limbs as chromatin source. ChIP sequencing data on chick limb cells analyzing promoter-associated histone marks allowed us to characterize the promoter of the chick JAG2 gene (Figure 8—figure supplement 1). We identified a putative regulatory region (−629 bp; −1023 bp) in the JAG2 promoter, which contained a MCAT element (CATTCC), the known binding motif for TEAD complexes (Davidson et al., 1988). We found that YAP was recruited to this regulatory region based on PCR (Figure 8B) and RT-q-PCR (Figure 8D) analyses. Further sequences, upstream of the JAG2 transcription initiation site, containing (−8481 −8960 bp) or not containing (−4500 bp −4894 bp) MCAT elements were not occupied by YAP (Figure 8A,B). This result showed that YAP was recruited to the JAG2 promoter region in limb fetal skeletal muscles. The YAP occupancy to this region was decreased in immobilized fetuses based on PCR (Figure 8C) and RT-q-PCR (Figure 8D) analyses, consistent with the decrease of JAG2 expression in muscle fibers in immobilization conditions (Figure 2). The YAP recruitment to JAG2 promoter (containing MCAT elements) provides a possible mechanism for the contraction-dependent control of JAG2 expression in fetal muscle fibers.
Discussion
Muscle contraction maintains the fetal muscle progenitor pool by preventing differentiation
The influence of muscle contraction has been extensively studied for skeleton (reviewed in Schwartz et al. (2013) and Shea et al. (2015)) but not for skeletal muscle development. We show here that mechanical signals are required to maintain the PAX7+ muscle progenitor pool during fetal myogenesis. However, muscle contraction does not change cell proliferation but rather affects the maintenance of PAX7+ cells by preventing their differentiation. Although chondrocyte proliferation is reduced in growth plates of long bone in the absence of muscle contraction (Roddy et al., 2011), skeletogenesis is also affected by proliferation-independent mechanisms after immobilization. Muscle contraction is necessary to maintain progenitors of the joint in an undifferentiated state and prevents their differentiation into chondrocytes (Kahn et al., 2009). Muscle contraction also controls chondrocyte convergence extension during cartilage development in zebrafish and mice (Shwartz et al., 2012). This indicates that progenitor cells of the musculoskeletal system are sensitive to mechanical signals generated by muscle contraction during development and respond to mechanical activity by several mechanisms. Interestingly in plants, physical forces contribute to stem cell maintenance acting on a master regulator (STM homeobox) of Arabidopsis shoot meristems, and this is achieved by a mechanism that is independent of cell proliferation (Landrein et al., 2015).
In the adult, changes in mechanical loading are known to cause variation of muscle size, but the contribution of satellite cells (adult muscle stem cells) to muscle atrophy or hypertrophy is a debated issue [reviewed in Brooks and Myburgh (2014)]. However, a reduction in the number of satellite cells has been described in adult muscle disuse/unloading animal models (Mitchell and Pavlath, 2004; Verdijk et al., 2012). Conversely, training exercises result in an increase of satellite cell number in humans (Crameri et al., 2004, 2007; Suetta et al., 2013). Moreover, physiological exercise has been shown to increases satellite cell numbers and to be beneficial for sarcopenic muscles [reviewed in Arthur and Cooley (2012)]. Thus, our data and the available information in literature indicate that muscle stem cells are sensitive to mechanical forces during muscle development, homeostasis and ageing. It remains to be determined whether similar molecular or cellular mechanisms are active in these diverse settings.
NOTCH signaling pathway acts downstream of mechanical signals during fetal myogenesis
Muscle paralysis causes a reduction of NOTCH activity and consequently muscle changes that are similar to those observed in NOTCH loss-of-function experiments. This shows that NOTCH activity is sensitive to mechanical signals during developmental myogenesis. Furthermore, ligand-induced NOTCH activation prevents the concomitant reduction of the fetal progenitor pool and increase muscle differentiation that is observed in the absence of muscle contraction. NOTCH components are also downregulated in bones of muscleless limbs of Spd mutant mice (Rolfe et al., 2014), although there is no described NOTCH loss-of-function phenotype in bones of muscleless limbs or immobilized embryos. NOTCH signaling is known to control the development of the cardiovascular system, which experiences mechanical forces. The NOTCH pathway has been proposed as an intermediary between mechanical forces and heart development [reviewed in Granados-Riveron and Brook (2012)], although the precise mechanotransduction pathway has not been identified. In adult muscle, NOTCH signaling has been extensively studied during muscle homeostasis, ageing and regeneration [reviewed in Mourikis and Tajbakhsh (2014)], and correlations between mechanical forces and NOTCH have been established (Carlson et al., 2009; Conboy et al., 2003). In summary, there is evidence for a change of NOTCH activity in different tissues during development and adult life upon muscle loading, but a functional link between mechanical signals and NOTCH has not been reported. We provide evidence that NOTCH signaling emerges as a molecular pathway that acts downstream of mechanical forces to regulate the pool of muscle progenitors during development. We believe that muscle activity acts on NOTCH ligand expression in fetal muscle fibers, since JAG2 expression is lost in fibers upon unloading and the blockade of NOTCH ligand function in muscle fibers suffices to decrease the number of adjacent muscle progenitors. In zebrafish, swimming-induced exercises lead to extensive transcriptional changes in fast muscles, including modification of the expression of NOTCH ligands, Dll1, Jag1 and Jag2 in addition to other NOTCH components (Palstra et al., 2014). Interestingly, on a molecular level, mechanical forces exerted by the signal-sending cell are required for ligand-induced NOTCH activation in a receiving cell that does not appear to sense forces directly (Gordon et al., 2015; Wang and Ha, 2013). Lastly, the NOTCH ligand Jagged1 rescues the Duchenne muscular dystrophy phenotype in zebrafish embryos and is upregulated in mildly affected dystrophin-deficient dogs (Vieira et al., 2015). All these data converge to the idea that mechanical forces provided by muscle contraction are sensed by NOTCH ligands in muscle fibers.
The molecular sensor of mechanical forces YAP regulates JAG2 transcription in post-mitotic muscle fibers
We show here that YAP activity is sensitive to mechanical stimuli in fetal muscles in vivo. The preferential loss of YAP protein in myonuclei and YAP target gene expression in differentiated muscle cells in immobilized fetuses indicate that muscle fibers directly sense mechanical signals. Our results do not provide evidence that muscle progenitors directly sense mechanical signals, since nuclear YAP protein, proliferation rate and apoptosis were not modified in PAX7+ cells in immobilization conditions. Moreover, within fetal muscles, we did not detect any expression of the YAP target genes, ANKRD1 and CTGF elsewhere than in MF20+ muscle fibers. YAP is, however, known to be expressed in activated satellite cells and has been shown to enhance satellite cell proliferation and to inhibit their differentiation in culture (Judson et al., 2012; Watt et al., 2010). Consistently, YAP is upregulated in alveolar rhabdomyosarcoma and is a potent modulator of rhabdomyosarcoma formation (Crose et al., 2014; Tremblay et al., 2014). However, in addition to YAP function in muscle cell proliferation, YAP was recently shown to be upregulated in muscle fibers by mechanical overload and to be critical for the size of skeletal muscle fibers in adult mice (Goodman et al., 2015; Watt et al., 2015), highlighting an additional YAP function in post-mitotic muscle fibers upon loading. YAP and NOTCH pathways have been shown to converge in many biological systems in mice (Li et al., 2012, Yimlamai et al., 2014, Manderfield et al., 2015). Oncogenic YAP variants activate the NOTCH ligand JAG1 and consequently NOTCH signaling in hepatocellular carcinoma cells (Tschaharganeh et al., 2013). Moreover, TEAD binds to regulatory sequences of the JAG1 gene in human breast cancer cells (Zhao et al., 2008). The current view is that YAP and NOTCH pathways converge to promote proliferation, cell fate and differentiation in a cell-autonomous manner in vertebrates. Using muscle unloading during fetal myogenesis, we provide evidence that YAP acts on muscle progenitors in a NOTCH–dependent and non-cell autonomous manner. This mechanism is reminiscent of that of the Drosophila YAP equivalent, Yorkie, during crystal cell differentiation in hematopoiesis, where Yorkie activates Serrate to induce responses in neighboring cells (Ferguson and Martinez-Agosto, 2014). We demonstrate a mechanistic link between YAP activity in muscle fibers and the regulation of the muscle progenitor pool and show that this is mediated by a transcriptional regulation of the NOTCH ligand JAG2 (Figure 9). It remains to be determined if a similar link exists between YAP and NOTCH ligands in adult muscle fibers that could explain changes in satellite cell number during unloading or uploading of muscles. We believe that the molecular mechanism that we identify during fetal development might be central to control muscle maintenance upon mechanical loading.
Materials and methods
Chick embryos
Request a detailed protocolFertilized chick eggs from commercial sources (White Leghorn, HAAS, Strasbourg, France and JA57 strain, Morizeau, Dangers, France) were incubated at 37.5°C. Chick embryos were staged according to days in ovo.
Drug administration
Request a detailed protocolDecamethonium bromide (DMB) (Sigma, France) and pancuronium bromide (PB) (Sigma) solutions were freshly prepared before each experiment at 12 mM or 11 mM, respectively, in Hank’s solution (Sigma) with Penicillin-Streptomycin at 1% (Gibco, France). The control solution was prepared using Hank’s solution with 1% of Penicillin-Streptomycin. 100 µl of DMB, PB or control solutions were administrated in chick embryos at E7.5 and E8.5. Embryos were fixed at E8 for the 12 hr time point, at E8.5 for the 24 hr time point and at E9.5 for the 48 hr time point.
Grafts of DELTA1-RCAS-expressing cells
Request a detailed protocolChick embryonic fibroblasts (CEFs) obtained from E10 chick embryos were transfected with DELTA1/RCAS using the Calcium Phosphate Transfection Kit (Invitrogen, France). Cell pellets of approximately 50–100 µm in diameter were grafted into limb buds of E3.5 embryos as previously described (Bonnet et al., 2010; Delfini et al., 2000). DELTA1/RCAS-grafted embryos were either harvested at E9.5 or treated with DMB or control solution and harvested at E9.5.
Construction of electroporation vectors and electroporation
Request a detailed protocolThe pT2AL-MLC-Tomato-2A-DELTA1/DN was designed as follows: the dominant negative form of DELTA1 was defined as a truncated form of DELTA1 that lacks the intracellular domain (Henrique et al., 1997) and was amplified by PCR from the DELTA1/RCAS (Delfini et al., 2000) using the following primer sequences: Fw GACTTCGAAATGGGAGGCCGCT and Rv CACGTGTTACTATCACCTGCAGGCCTCG. The DELTA1/DN PCR product was inserted in the pT2AL-MLC-Tomato-2A-GFP (Bourgeois et al., 2015) after GFP removal to obtain pT2AL-MLC-Tomato-2A-DELTA1/DN (named as pT2AL-MLC-Tomato-DELTA1/DN). The pT2AL-MLC-Tomato-2A-mYapS112A was designed as follows: mYapS112A (Xin et al. 2013) was amplified by PCR from the mYapS112A/RCAS (McKey et al., 2016) using the following primer sequences: Fw CAATTCGAAATGGAGCCCGCG and Rv GGCCACGTGCTATAACCACGTGAGAAA. The mYapS112A PCR product was inserted in the pT2AL-MLC-Tomato-2A-GFP (Bourgeois et al., 2015) after GFP removal to obtain pT2AL-MLC-Tomato-2A-mYapS112A (named as pT2AL-MLC-Tomato-YapS112A). Forelimb somite electroporation was performed as previously described (Wang et al., 2011). The DNA solution was composed of the pT2AL-MLC-Tomato-DELTA1/DN or pT2AL-MLC-Tomato-YapS112A vectors and a transient transposase-containing vector pCAGGS-T2TP, at a molar ratio of 3:1. This vector set allows the stable integration of the MLC-Tomato-DELTA1/DN or the MLC-Tomato-YapS112A cassettes into the chick genome. Embryos electroporated with pT2AL-MLC-Tomato-YapS112A were immobilized with DMB treatment.
Myoblast cultures
Request a detailed protocolPrimary myoblasts were obtained from hindlimbs of E10 chick embryos, as previously described (Havis et al., 2012). DMB and PB were applied to proliferating myoblast cultures in high-serum conditions (10%), at a final concentration of 50 µM and 5 µM, respectively, for 48 hr. Myoblasts were then analyzed by immunohistochemistry for PAX7+ muscle progenitors and MF20+ differentiated cells and for muscle gene expression by RT-q-PCR. To force YAP activity in muscle cells, myoblasts were transfected with YapS112A/RCAS (McKey et al., 2016). Muscle cells were amplified in high-serum conditions until confluence, collected and analyzed for gene expression by RT-q-PCR. To obtain differentiated muscle cells that overexpress YapS112A, chick fetal myoblasts were transfected with pT2AL-MLC-Tomato-YapS112A or pT2AL-MLC-Tomato as control and cultured for 4 days. Cells were collected into PBS 1X 10% fetal bovine serum (FBS), stained with DAPI to exclude dead cells and purified via FACS Aria gating on the Tomato+ cell fraction.
RNA extraction and quantitative real-time PCR
Request a detailed protocolTotal RNAs were extracted from control limbs, experimental limbs or primary fetal myoblast cultures. 500 ng to 1 µg of RNA was reverse-transcribed using the High-Capacity Retrotranscription kit (Applied Biosystems, France). RT-q-PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems). Primer sequences used for RT-q-PCR are listed in Supplementary file 1. The relative mRNA levels were calculated using the 2^-ΔΔCt method (Livak and Schmittgen, 2001). The ΔCts were obtained from Ct normalized with GAPDH and RPS17 levels in each sample. Twelve DMB, PB or control limbs, originating from four independent experiments, were used as independent RNA samples. Six samples of cultured myoblasts originating from three independent experiments were used as independent RNA samples for DMB, PB, YapS112A/RCAS or pT2AL-MLC-Tomato-YapS112A experiments. Each RNA sample was analyzed in duplicate. Errors bars in RT-q-PCR results represent standard error of the mean.
EdU experiments
Request a detailed protocolFifty microliters of EdU (Invitrogen) solution (5 mg/ml) was injected into the circulation in E9.5 chick embryos for 1.5 hr. Embryos were then fixed and immunohistochemistry was performed on limb transverse sections.
Immunohistochemistry
Request a detailed protocolForelimbs of control or manipulated (DMB, PB, DELTA1/RCAS, DELTA1/RCAS+DMB, pT2AL-MLC-Tomato-DELTA1/DN, pT2AL-MLC-Tomato-YapS112A+DMB) chick embryos were fixed in 4% paraformaldehyde overnight at 4°C and then processed in gelatin/sucrose for 12 µm cryostat sections. The monoclonal antibodies, MF20 that recognizes sarcomeric myosin heavy chains and PAX7 that recognizes muscle progenitors, developed by D.A. Fischman and A. Kawakami, respectively, were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology Iowa City, IA 52242. The Desmin antibody was obtained from Sigma (dilution 1/100). The YAP mouse monoclonal antibody was obtained from Santa Cruz Biotechnology (dilution 1/50). The MEP21 antibody originates from (McNagny et al., 1997). Proliferation analysis (EdU) was performed using the Click-iT kit (Thermo Fisher Scientific, France). Apoptosis was detected using the ApoTag kit (Millipore, France). Secondary antibodies were conjugated with Alexa-488 or Alexa-555 (Invitrogen). Nuclei were detected with Hoechst staining (Molecular Probes).
In situ hybridization
Request a detailed protocolChick forelimbs of control or manipulated (DMB, PB, DELTA1/RCAS, DELTA1/RCAS+DMB, pT2AL-MLC-Tomato-DELTA1/DN, pT2AL-MLC-Tomato-YapS112A+DMB) embryos were fixed in Farnoy (60% ethanol, 30% formaldehyde (stock at 37%) and 10% acetic acid) overnight at 4°C, and processed for in situ hybridization on wax tissue sections, as previously described (Wang et al., 2010). The digoxigenin-labeled mRNA probes were obtained as follows: DLL1, JAG2 and MYOD (Delfini et al., 2000); HES5 (Henrique et al., 1997). CTGF (EST clones) and YAP1 (McKey et al., 2016) probes were linearized with NotI and SacII and synthetized with T3 and SP6, respectively. ANKRD1 probe was obtained by PCR from E9.5 limb tissues using the following primers: Fw: TGGCTCACGGGAAGGAGAAG; Rv: GGTGCTCGGCACAGTCG, cloned into the pCRII-TOPO vector (Invitrogen), linearized with KpnI and synthetized with T7.
Chromatin immunoprecipitation assay
Request a detailed protocolChIP assay was performed as previously described (Havis et al., 2012). Eight limbs from E9.5 chick embryos were homogenized using a mechanical disruption device (Lysing Matrix A, Fast Prep MP1, 40 s at 6 m/s). 10 µg of the YAP rabbit polyclonal antibody (Santa Cruz Biotechnology) or 10 µg of the anti-acetylated histone H4 (AcH4) antibody (Upstate Biotechnology) was used to immunoprecipitate 20 µg of sonicated chromatin. ChIP products were analyzed by PCR to amplify three regions upstream the JAG2 coding sequence or by RT-q-PCR to amplify the region 1 (Figure 8A). The primer list is displayed in Supplementary file 1. ChIP assay was performed with or without DMB treatment in two independent biological replicates.
Image capturing
Request a detailed protocolAfter immunohistochemistry or in situ hybridization experiments, images were obtained using a Nikon epifluorescence microscope, a Leica DMI600B fluorescence microscope or a Leica SP5 confocal system.
Cell number and muscle area measurements
Request a detailed protocolAll cell number and muscle area measurements were performed using the free software ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2012). To quantify the number of PAX7+ cells in limb muscles of DMB- and PB-treated fetuses, the number of PAX7+ cells was counted per unit area in dorsal and ventral muscles on three sections of each three different limbs originating from either DMB-treated, PB- treated or control embryos. The quantification of PAX7+ cells and muscle area (delineated with PAX7 and MF20 expression domains) in DELTA1/RCAS-grafted embryos was performed in three sections of each grafted (right) and contralateral (left) limbs of three immobilized and three control embryos. Quantification was performed in ectopic DLL1-expressing muscles of grafted right limbs and compared with equivalent muscles of the contralateral left limbs originating from the same embryos. The quantification of the Tomato+/MF20+ cells, MF20+ cells, PAX7+ cells and muscle area in pT2AL-MLC-Tomato-DELTA1/DN electroporated embryos was performed in five sections of each electroporated (right) and contralateral (left) of four embryos. Quantification was performed in Tomato-expressing muscles of electroporated right limbs and compared with equivalent muscles of the contralateral left limbs originating from the same embryos. The quantification of PAX7+ cells in pT2AL-MLC-Tomato-YapS112A electroporated and immobilized fetuses was performed in four sections of each electroporated (right) and contralateral (left) of four immobilized fetuses. Quantification was performed in Tomato-expressing muscles of electroporated right limbs and compared with equivalent muscles of the contralateral left limbs originating from the same embryos. Hoechst+ nuclei overlapping with MYOD expression were counted in four different muscles over four different sections and compared with the total number of Hoechst+ nuclei in paralyzed (DMB 48 hrs) and control muscles.
Statistical analyses
Request a detailed protocolData were analyzed using non-parametric two-tailed tests, Mann-Withney test for unpaired samples or Wilcoxon test for paired samples using Graphpad Prism V6. Results were shown as means ± standard deviations or standard errors of the mean, depending on the size of the samples. The p-values are indicated either with the number on the graphs or with asterisks. Asterisks indicate the different p-values *p<0.05, **p<0.01 and ***p<0.001.
References
-
Notch signaling: simplicity in design, versatility in functionDevelopment 138:3593–3612.https://doi.org/10.1242/dev.063610
-
The effect of physiological stimuli on sarcopenia; impact of Notch and Wnt signaling on impaired aged skeletal muscle repairInternational Journal of Biological Sciences 8:731–760.https://doi.org/10.7150/ijbs.4262
-
Molecular aging and rejuvenation of human muscle stem cellsEMBO Molecular Medicine 1:381–391.https://doi.org/10.1002/emmm.200900045
-
Why is delta endocytosis required for effective activation of notch?Developmental Dynamics 235:886–894.https://doi.org/10.1002/dvdy.20683
-
Alveolar rhabdomyosarcoma-associated PAX3-FOXO1 promotes tumorigenesis via Hippo pathway suppressionJournal of Clinical Investigation 124:285–296.https://doi.org/10.1172/JCI67087
-
Delta 1-activated notch inhibits muscle differentiation without affecting Myf5 and Pax3 expression in chick limb myogenesisDevelopment 127:5213–5224.
-
The impact of mechanical forces in heart morphogenesisCirculation: Cardiovascular Genetics 5:132–142.https://doi.org/10.1161/CIRCGENETICS.111.961086
-
Paralysis and growth of the musculoskeletal system in the embryonic chickJournal of Morphology 206:45–56.https://doi.org/10.1002/jmor.1052060105
-
Notch signalling acts in postmitotic avian myogenic cells to control MyoD activationDevelopment 128:107–116.
-
The Hippo pathway member Yap plays a key role in influencing fate decisions in muscle satellite cellsJournal of Cell Science 125:6009–6019.https://doi.org/10.1242/jcs.109546
-
Muscle contraction is necessary to maintain joint progenitor cell fateDevelopmental Cell 16:734–743.https://doi.org/10.1016/j.devcel.2009.04.013
-
Pax3/Pax7 mark a novel population of primitive myogenic cells during developmentGenes & Development 19:1426–1431.https://doi.org/10.1101/gad.345505
-
Muscle ankyrin repeat proteins: their role in striated muscle function in health and diseaseCritical Reviews in Clinical Laboratory Sciences 48:269–294.https://doi.org/10.3109/10408363.2011.643857
-
Matricellular proteins of the Cyr61/CTGF/NOV (CCN) family and the nervous systemFrontiers in Cellular Neuroscience 9:237.https://doi.org/10.3389/fncel.2015.00237
-
Thrombomucin, a novel cell surface protein that defines thrombocytes and multipotent hematopoietic progenitorsJournal of Cell Biology 138:1395–1407.https://doi.org/10.1083/jcb.138.6.1395
-
Skeletal muscle atrophy leads to loss and dysfunction of muscle precursor cellsAJP: Cell Physiology 287:C1753–C1762.https://doi.org/10.1152/ajpcell.00292.2004
-
Distinct contextual roles for Notch signalling in skeletal muscle stem cellsBMC Developmental Biology 14:2.https://doi.org/10.1186/1471-213X-14-2
-
Mechanobiology of embryonic skeletal development: Insights from animal modelsBirth Defects Research Part C: Embryo Today: Reviews 90:203–213.https://doi.org/10.1002/bdrc.20184
-
The biology of YAP/TAZ: hippo signaling and beyondPhysiological Reviews 94:1287–1312.https://doi.org/10.1152/physrev.00005.2014
-
Skeletal muscle stem cells in developmental versus regenerative myogenesisJournal of Internal Medicine 266:372–389.https://doi.org/10.1111/j.1365-2796.2009.02158.x
-
Reduced satellite cell numbers with spinal cord injury and aging in humansMedicine & Science in Sports & Exercise 44:2322–2330.https://doi.org/10.1249/MSS.0b013e3182667c2e
-
Yap is a novel regulator of C2C12 myogenesisBiochemical and Biophysical Research Communications 393:619–624.https://doi.org/10.1016/j.bbrc.2010.02.034
-
Genome-wide association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growthNature Cell Biology 17:1218–1227.https://doi.org/10.1038/ncb3216
-
During secondary myotube formation, primary myotubes preferentially absorb new nuclei at their endsDevelopmental Dynamics 204:168–177.https://doi.org/10.1002/aja.1002040207
-
TEAD mediates YAP-dependent gene induction and growth controlGenes & Development 22:1962–1971.https://doi.org/10.1101/gad.1664408
Article and author information
Author details
Funding
Agence Nationale de la Recherche (ANR-12-BSV1-0038)
- Delphine Duprez
AFM-Téléthon (AFM 16752/16826)
- Delphine Duprez
Fondation pour la Recherche Médicale (DEQ20140329500)
- Delphine Duprez
Centre National de la Recherche Scientifique
- Delphine Duprez
Institut National de la Santé et de la Recherche Médicale
- Delphine Duprez
Université Pierre et Marie Curie
- Delphine Duprez
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank Pascal de Santa Barbara (Montpellier, France) and Thierry Jaffredo (Paris, France) for reagents, Laurence Petit (Paris, France) for help with FACS sorting. We thank laboratory members for comments on the manuscript and Sophie Gournet for illustrations.
Copyright
© 2016, Esteves de Lima et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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